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Algemene gegevens
PPS-nummer
Titel
Topsector en innovatiethema
Projectleider (onderzoek)
PPS-coördinator (namens
private partij)
Contactpersoon overheid
Status (lopend of afgerond)
Type onderzoek (F, T of V)
Werkelijke startdatum
Werkelijke einddatum
Korte omschrijving inhoud
EZ-2012-12
Improving Doubled-haploid (DH) embryo production
systems by inhibitors of histone deacetylases
Tuinbouw & Uitgangsmaterialen, Meer met Minder
Kim Boutilier
Iris Heidmann
Jan van Vliet
Running / lopend
Strategic / T
1-01-2014
1-03-2018
The goals of the project are 1) to determine how
inhibition of histone deacetylase (HDAC) activity induces
cell totipotency and enhances embryo quality in
microspore culture and 2) to optimise the practical
applications of this technology.
Highlights
 An isolated microspore culture system was developed for the model plant
arabidopsis. This system will be used to study the molecular-genetic pathways
regulating haploid embryo development from pollen, with specific emphasis on
the role of histone acetylation in this process.
 Secondary chemical genomics screens were performed to identify compounds
that enhance the positive effect of the HDAC inhibitor trichostatin A on haploid
embryo induction. A strong enhancer compound was identified.