mediated by phosphofructokinase deletion Boro

University of Groningen
Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by
phosphofructokinase deletion
Borodina, Irina; Siebring, Jeroen; Zhang, Jie; Smith, Colin P.; van Keulen, Geertje;
Dijkhuizen, Lubbert; Nielsen, Jens
Published in:
The Journal of Biological Chemistry
DOI:
10.1074/jbc.M803105200
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to
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Document Version
Publisher's PDF, also known as Version of record
Publication date:
2008
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Borodina, I., Siebring, J., Zhang, J., Smith, C. P., van Keulen, G., Dijkhuizen, L., & Nielsen, J. (2008).
Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion. The
Journal of Biological Chemistry, 283(37), 25186-25199. DOI: 10.1074/jbc.M803105200
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Download date: 18-07-2017
ORF
SCO1174
SCO5515
SCO4366
SCO4984
SCO0546
RNA (SCO4729/SCO4654/SCO1478)
SCO3182
SCO7443
SCO5424
SCO5356
SCO7311
SCO5535
SCO2736
SCO1916
SCO0199
Reaction
T3 + NAD -> NADH + DGLYCERATE
3PG + NAD -> NADH + PHP
PHP + GLU -> AKG + 3PSER
AKG + TYR <-> 4HPP + GLU
PYR + ATP + CO2 -> ADP + OA + PI
0.600 ATP + 0.826 GTP + 1.031 CTP + 0.662 UTP + 1.25 ATP -> 1.25 ADP + 1.25 PI + RNA + 3.119 PPI
G1P + UTP <-> UDPG + PPI
G6P <-> G1P
AC + ATP <-> ACETYLP + ADP
HSER + ATP -> ADP + PHSER
LYS -> CADA + CO2
SMMALONYLCOA -> PROPIONYLCOA + CO2
ACCOA + OA -> COA + CIT
TETRAHYDRODIPICOLINATE + SUCCOA -> SAOPIM + COA
ACAL + NADH <-> ETH + NAD