Problemen bij identificeren blootstelling enzymen
advertisement
" # ! IRAS: Institute for Risk Assessment Sciences Utrecht University ! ! interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences Utrecht University $ % # 30 Leegstorten zakje additieven 20 Vullen emmer onder stortkoker 15 Persoon loopt door stofwolk bij silo na storten Leegstorten zak meel 10 Leegstorten zakjes additieven Vegen met bezem 5 Afweegmachine Afweegmachine Afweegmachine 1905/04/01 1905/01/01 1904/10/01 1904/07/01 1904/04/01 1904/01/01 1903/10/01 1903/07/01 1903/04/01 1903/01/01 1902/10/01 1902/07/01 1902/04/01 1902/01/01 1901/10/01 1901/07/01 1901/04/01 1901/01/01 1900/10/01 1900/07/01 1900/04/01 0 1900/01/01 IRAS: Institute for Risk Assessment Sciences 3 Concentratie stof (mg/m ) 25 interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences Utrecht University % & ! IRAS: Institute for Risk Assessment Sciences $ ! ! ' () interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences HALOgen immunostaining (Tovey et al.) Ontwikkeld voor amylase en tarwe Utrecht University IRAS: Institute for Risk Assessment Sciences particles collected on adhesive film in passive sampler the particles are then sandwiched between the adhesive film and the protein-binding membrane particle protein-binding membrane extracted allergen binds to membrane view HALO of stain through adhesive film immunostain allergen with IgE or m-Ab through membrane ‘halos’ around Cat particle & Alternaria spore interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences % Utrecht University ) # ' 30 Leegstorten zakje additieven 20 Vullen emmer onder stortkoker 15 Persoon loopt door stofwolk bij silo na storten Leegstorten zak meel 10 Leegstorten zakjes additieven Vegen met bezem 5 Afweegmachine Afweegmachine Afweegmachine 1905/04/01 1905/01/01 1904/10/01 1904/07/01 1904/04/01 1904/01/01 1903/10/01 1903/07/01 1903/04/01 1903/01/01 1902/10/01 1902/07/01 1902/04/01 1902/01/01 1901/10/01 1901/07/01 1901/04/01 1901/01/01 1900/10/01 1900/07/01 1900/04/01 0 1900/01/01 IRAS: Institute for Risk Assessment Sciences 3 Concentratie stof (mg/m ) 25 interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences * Utrecht University Job Tasks bijzonderheden LEV IRAS: Institute for Risk Assessment Sciences Blanco Amylase positieve deeltjes 0 Tarwe positieve deeltjes - ingredient weigher Afwegen ingredienten Laminaire flow wall 5-7 - ingredient weigher Afwegen ingredienten Laminaire flow wall 8-10 - ingredient weigher weighing ingredients ingredient weigher wieghing ingredients (incl pure enzyme) magazijn medewerker storten van bulk zakgoed in silo erg actieve schepper, veroorzaakt veel Laminaire flowe >20-60, wall overloaded zeer korte meting Ringventilatie >20-100 0- Storter zakgoed (retour) storten van zakgoed pre-mix met amylase in stortkoker 7-25 - Storter zakgoed storten van zakgoed in stortkoker (retour)poederfabriek 4-5 - 0 - 1-3 - Storter zakgoed (retour) poederfabriek Tapper storten van zakgoed in stortkoker tapper aftappen van zakgoed weinig ingrijpen 0 >100 tapper aftappen van zakgoed met sampler geknoeid overloaded positief >100 geen amylase aftappen van zakgoed interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences Kenmerken α-amylase in de bakerij omgeving Utrecht University • Amylase toegevoegd als granulaat enzyme aan tarwe meel, in een verhouding van ~ 1/100,000 w/w IRAS: Institute for Risk Assessment Sciences • Bij stofcentraties van > 1-2 mg/m3 >90% is tarwe meel stof 1 op de 100,000 stof deeltjes is een enzyme partikel Sampling concentreren van partikels van ~ 0.2 - 1 m3 lucht op filter ~ 0.1 – 5 mg tarwe ~ 0.1 – 5 ng α-amylase interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences ) ! Utrecht University # ( IRAS: Institute for Risk Assessment Sciences # ! ( interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences # # Utrecht University + , # - ! IRAS: Institute for Risk Assessment Sciences # # interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences . ) .) Utrecht University 0 ! &1 ! IRAS: Institute for Risk Assessment Sciences 2 ( & & .) ! & (3 4*/ (5 # / ' LFIA - tube interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences type .) 6 Utrecht University 7 ' ! ! 7 8 IRAS: Institute for Risk Assessment Sciences & # 9 a) b) c) d) e) 7 0 7 7 / 8 ! ! interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences #: # 8 ! .) Utrecht University . ; <6 =7 IRAS: Institute for Risk Assessment Sciences & & 9 ) >7 6 ! "#$ %&' ( )* +! "&' ( )* +! "&' ( , ? *( @7 & / ! %% !! interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences / !. / IRAS: Institute for Risk Assessment Sciences Utrecht University . ! & & & 6 A ! !" ' ! =B 9 9 2 ! .) interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences 8 ! .) 6 IRAS: Institute for Risk Assessment Sciences Utrecht University ;C 2 ! =; "' @BBB 9 ! ( AB ! ( 2 .) interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences .) ! ! Utrecht University Control: IRAS: Institute for Risk Assessment Sciences goat antirabbit IgG Test line: Rabbit IgG antiamylase 6 & & & & => @H ! ! " 0&A D => =@ =A E 0I0 " 0&A F G =B =H =; E =F =G E ; >B&>H E A == =D E HB interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences 2 >; 8 ! .) Utrecht University IRAS: Institute for Risk Assessment Sciences 0 # * " 0&A 1 FHC * >AC BC 0I0 BC 9 () 2 interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences .) J; 4 I$ .) Utrecht University IRAS: Institute for Risk Assessment Sciences Pakket voor het verzamelen van veegmonsters - PBT (PBS+0.05% Tween20) voor extrahatie (in Greiner 15 ml buisje) - Greiner 15 ml buisjes (art. no. 18 8271) - PBT (PBS+0.05% Tween20) voor bevochtiging filter: (in Sarstedt 2 ml buisje) - Papieren filter (55 mm, Schleicher & Schuell, Dassel, Germany) - Sarstedt 3.5 ml pipet (art.no. 86 1171) - Sarstedt 3.5 ml pipet (art.no. 86 1172) Pakket voor het verzamelen van grondstofmon - PBT (PBS+0.05% Tween20) - Greiner container met lepel (art. no. 44 - Sarstedt 3.5 ml pipet (art.no. 86 1171) Pakket voor veegmonster Pakket voor grondstofmonster interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences 3 .) # Utrecht University 4 # IRAS: Institute for Risk Assessment Sciences + $ 4 ) ? 7 7 # ! 6 B@B&>;@GHHG K 7 7 interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences 0 Utrecht University 8 4 ! # IRAS: Institute for Risk Assessment Sciences # .) # - interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences α-amylase allergeen blootstelling Implicaties van de bevindingen Utrecht University Amylase zit in ‘relatief grote partikels’: die bevatten ~0.04 – 2.0 ng amylase IRAS: Institute for Risk Assessment Sciences Verhoogd risico op sensitisatie aangetoond bij lage nivo’s (70% < LOD van de ELISA) ± 5 ng/m3 Dit kan dus Veroorzaakt door inhalatie van gemiddeld 1-10 partikels/dag Dit heeft een inherent grote variatie in de blootstellingsmetingen tot gevolg interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences ) Utrecht University 0 # IRAS: Institute for Risk Assessment Sciences 4 , - # # $ interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences 3 Utrecht University IRAS: Institute for Risk Assessment Sciences Tim Meijster Jose Jacobs Jelena Bogdanovic Gert Doekes Dick Heederik MOCALEX partners: A&F Wageningen Marjo Koets Aart van Amerongen BGFA Bochum Ingrid Sander Liesbeth Preller Evelyn Tjoe-Nij Erik Tielemans Karolinska Institute Anne Renstrom interfacultary institute within the faculties Medicine, Veterinary Medicine and within the faculty of Science the departments Biology and Pharmaceutical Sciences