Gene PaVE DNA Sequence Optimized DNA Sequence Protein
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Gene HPV6 E1 PaVE DNA Sequence Optimized DNA Sequence Protein Sequence ATGGCGGACGATTCAGGTACAGAAAATGAG GGGTCTGGGTGTACAGGATGGTTTATGGTAG AAGCTATAGTGCAACACCCAACAGGTACACA AATATCAGACGATGAGGATGAGGAGGTGGA GGACAGTGGGTATGACATGGTGGACTTTATT GATGACAGCAATATTACACACAATTCACTGG AAGCACAGGCATTGTTTAACAGGCAGGAGGC GGACACCCATTATGCGACTGTGCAGGACCTA AAACGAAAGTATTTAGGTAGTCCATATGTTA GTCCTATAAACACTATAGCCGAGGCAGTGGA AAGTGAAATAAGTCCACGATTGGACGCCATT AAACTTACAAGACAGCCAAAAAAGGTAAAGC GACGGCTGTTTCAAACCAGGGAACTAACGGA CAGTGGATATGGCTATTCTGAAGTGGAAGCT GGAACGGGAACGCAGGTAGAGAAACATGGC GTACCGGAAAATGGGGGAGATGGTCAGGAA AAGGACACAGGAAGGGACATAGAGGGGGA GGAACATACAGAGGCGGAAGCGCCCACAAA CAGTGTACGGGAGCATGCAGGCACAGCAGG AATATTGGAATTGTTAAAATGTAAAGATTTAC GGGCAGCATTACTTGGTAAGTTTAAAGAATG CTTTGGGCTGTCTTTTATAGATTTAATTAGGC CATTTAAAAGTGATAAAACAACATGTTTAGAT TGGGTGGTAGCAGGGTTTGGTATACATCATA GCATATCAGAGGCATTTCAAAAATTAATTGA GCCATTAAGTTTATATGCACATATACAATGGC TAACAAATGCATGGGGAATGGTATTGTTAGT ATTATTAAGATTTAAAGTAAATAAAAGTAGA AGTACCGTTGCACGTACACTTGCAACGCTATT AAATATACCTGAAAACCAAATGTTAATAGAG CCACCAAAAATACAAAGTGGTGTTGCAGCCC TGTATTGGTTTCGTACAGGTATATCAAATGCC AGTACAGTTATAGGGGAAGCACCAGAATGG ATAACACGCCAAACAGTTATTGAACACGGGT TGGCAGACAGTCAGTTTAAATTAACAGAAAT GGTGCAGTGGGCGTATGATAATGACATATGC GAGGAGAGTGAAATTGCATTTGAATATGCAC AAAGGGGAGATTTTGATTCTAATGCACGAGC ATTTTTAAATAGCAATATGCAGGCAAAATATG TGAAAGATTGTGCAACTATGTGTAGACATTAT AAACATGCAGAAATGAGGAAGATGTCTATAA AACAATGGATAAAACATAGGGGTTCTAAAAT AGAAGGCACAGGAAATTGGAAACCAATTGTA CAATTCCTACGACATCAAAATATAGAATTCAT TCCTTTTTTAACTAAATTTAAATTATGGCTGCA CGGTACGCCAAAAAAAAACTGCATAGCCATA GTAGGCCCTCCAGATACTGGGAAATCGTACT TTTGTATGAGTTTAATAAGCTTTCTAGGAGGT ACAGTTATTAGTCATGTAAATTCCAGCAGCCA TTTTTGGTTGCAACCGTTAGTAGATGCTAAGG TAGCATTGTTAGATGATGCAACACAGCCATG TTGGATATATATGGATACATATATGAGAAATT TGTTAGATGGTAATCCTATGAGTATTGACAG AAAGCATAAAGCATTGACATTAATTAAATGTC CACCTCTGCTAGTAACGTCCAACATAGATATT ACTAAAGAAGATAAATATAAGTATTTACATAC TAGAGTAACAACATTTACATTTCCAAATCCAT TCCCTTTTGACAGAAATGGGAATGCAGTGTA TGAACTGTCAAATACAAACTGGAAATGTTTTT TTGAAAGACTGTCGTCAAGCCTAGACATTCA GGATTCTGAGGACGAGGAAGATGGAAGCAA TAGCCAAGCGTTTAGATGCGTGCCAGGAACA GTTGTTAGAACTTTATGA ATGGCAGACGATTCAGGGACAGAAAATGAG GGGAGCGGGTGTACTGGGTGGTTTATGGTG GAGGCTATTGTGCAGCATCCTACTGGAACCC AGATCTCCGACGATGAGGACGAGGAAGTGG AAGATTCTGGGTACGACATGGTCGATTTTATC GACGATTCTAACATTACACACAATAGTCTGGA GGCTCAGGCACTGTTCAACAGACAGGAAGCA GACACACATTATGCCACTGTGCAGGATCTGA AGAGGAAATACCTGGGCAGTCCCTATGTGTC ACCTATCAATACCATTGCCGAGGCTGTCGAGT CTGAAATCAGTCCACGACTGGACGCCATCAA GCTGACACGGCAGCCCAAGAAAGTGAAGCG GAGACTGTTTCAGACCCGCGAGCTGACAGAT AGCGGGTACGGATATTCCGAGGTCGAAGCC GGCACAGGGACTCAGGTGGAGAAACACGGA GTCCCAGAAAACGGAGGGGACGGACAGGAG AAGGACACAGGACGCGATATCGAAGGCGAG GAACACACAGAGGCAGAAGCCCCCACTAATA GCGTGCGAGAGCATGCCGGCACTGCTGGGA TCCTGGAACTGCTGAAGTGCAAAGACCTGCG GGCCGCTCTGCTGGGCAAGTTCAAAGAGTGT TTTGGGCTGAGTTTCATCGATCTGATTAGACC TTTCAAGTCAGACAAAACCACATGCCTGGATT GGGTGGTCGCTGGATTTGGCATCCACCATTC CATTTCTGAGGCATTCCAGAAGCTGATCGAA CCACTGTCCCTGTACGCACACATTCAGTGGCT GACTAACGCCTGGGGCATGGTGCTGCTGGTC CTGCTGAGGTTTAAGGTGAACAAGAGTAGGT CAACCGTCGCTCGCACCCTGGCAACACTGCT GAACATCCCCGAGAATCAGATGCTGATCGAA CCCCCTAAGATTCAGAGCGGAGTGGCAGCCC TGTATTGGTTCCGCACAGGGATCTCAAACGC TAGCACTGTGATTGGAGAGGCACCTGAATGG ATCACTCGGCAGACCGTCATTGAGCACGGCC TGGCCGACTCTCAGTTTAAGCTGACCGAAAT GGTGCAGTGGGCTTACGACAACGATATCTGT GAGGAAAGCGAGATTGCCTTCGAATATGCTC AGAGAGGGGACTTTGATTCAAATGCTAGGGC ATTCCTGAACAGCAATATGCAGGCCAAGTAC GTGAAAGATTGCGCTACCATGTGTCGCCACT ATAAGCATGCCGAGATGCGAAAGATGAGCAT CAAACAGTGGATTAAGCATAGAGGATCCAAA ATCGAAGGGACAGGAAACTGGAAGCCTATT GTGCAGTTTCTGAGGCACCAGAATATCGAGT TCATTCCTTTTCTGACCAAGTTCAAACTGTGG CTGCATGGCACACCAAAGAAAAACTGCATCG CCATTGTGGGGCCACCCGACACAGGAAAATC TTACTTTTGTATGTCCCTGATCTCTTTCCTGGG AGGCACTGTGATTAGTCACGTCAATAGCTCCT CTCATTTTTGGCTGCAGCCCCTGGTGGACGCA AAGGTCGCCCTGCTGGACGATGCAACTCAGC CTTGCTGGATCTACATGGATACCTATATGAG GAACCTGCTGGACGGCAATCCAATGAGCATC GATCGCAAGCACAAAGCTCTGACCCTGATCA AGTGTCCTCCACTGCTGGTGACCTCCAACATC GACATTACAAAGGAGGATAAGTACAAATATC TGCATACTCGAGTGACTACCTTCACCTTTCCC AATCCTTTCCCATTTGATCGGAACGGCAATGC CGTCTACGAGCTGTCTAACACCAATTGGAAG TGCTTCTTTGAACGGCTGAGTTCAAGCCTGG ACATCCAGGATAGCGAGGACGAGGAAGATG GCAGCAACTCCCAGGCCTTCCGGTGTGTGCC AGGAACTGTGGTCAGAACCCTGTGA MADDSGTENEGSGCTGWFMVEAI VQHPTGTQISDDEDEEVEDSGYDM VDFIDDSNITHNSLEAQALFNRQEAD THYATVQDLKRKYLGSPYVSPINTIAE AVESEISPRLDAIKLTRQPKKVKRRLF QTRELTDSGYGYSEVEAGTGTQVEK HGVPENGGDGQEKDTGRDIEGEEH TEAEAPTNSVREHAGTAGILELLKCK DLRAALLGKFKECFGLSFIDLIRPFKSD KTTCLDWVVAGFGIHHSISEAFQKLI EPLSLYAHIQWLTNAWGMVLLVLLR FKVNKSRSTVARTLATLLNIPENQML IEPPKIQSGVAALYWFRTGISNASTVI GEAPEWITRQTVIEHGLADSQFKLTE MVQWAYDNDICEESEIAFEYAQRG DFDSNARAFLNSNMQAKYVKDCAT MCRHYKHAEMRKMSIKQWIKHRGS KIEGTGNWKPIVQFLRHQNIEFIPFLT KFKLWLHGTPKKNCIAIVGPPDTGKS YFCMSLISFLGGTVISHVNSSSHFWL QPLVDAKVALLDDATQPCWIYMDT YMRNLLDGNPMSIDRKHKALTLIKC PPLLVTSNIDITKEDKYKYLHTRVTTFT FPNPFPFDRNGNAVYELSNTNWKCF FERLSSSLDIQDSEDEEDGSNSQAFR CVPGTVVRTL Page 1 of 48 % Nucleotide Change 41.3% Gene HPV6 E2 HPV6 E4 HPV6 E5A HPV6 E5B HPV6 E6 PaVE DNA Sequence Optimized DNA Sequence Protein Sequence ATGGAAGCAATAGCCAAGCGTTTAGATGCGT GCCAGGAACAGTTGTTAGAACTTTATGAAGA AAACAGTACTGACCTACACAAACATGTATTGC ATTGGAAATGCATGAGACATGAAAGTGTATT ATTATATAAAGCAAAACAAATGGGCCTAAGC CACATAGGAATGCAAGTAGTGCCACCATTAA AGGTGTCCGAAGCAAAAGGACATAATGCCAT TGAAATGCAAATGCATTTAGAATCATTATTAA GGACTGAGTATAGTATGGAACCGTGGACATT ACAAGAAACAAGTTATGAAATGTGGCAAACA CCACCTAAACGCTGTTTTAAAAAACGGGGCA AAACTGTAGAAGTTAAATTTGATGGCTGTGC AAACAATACAATGGATTATGTGGTATGGACA GATGTGTATGTGCAGGACAATGACACCTGGG TAAAGGTGCATAGTATGGTAGATGCTAAGGG TATATATTACACATGTGGACAATTTAAAACAT ATTATGTAAACTTTGTAAAAGAGGCAGAAAA GTATGGGAGCACCAAACATTGGGAAGTATGT TATGGCAGCACAGTTATATGTTCTCCTGCATC TGTATCTAGCACTACACAAGAAGTATCCATTC CTGAATCTACTACATACACCCCCGCACAGACC TCCACCCTTGTGTCCTCAAGCACCAAGGAAG ACGCAGTGCAAACGCCGCCTAGGAAACGAG CACGAGGAGTCCAACAGTCCCCTTGCAACGC CTTGTGTGTGGCCCACATTGGACCCGTGGAC AGTGGAAACCACAACCTCATCACTAACAATC ACGACCAGCACCAAAGACGGAACAACAGTA ACAGTTCAGCTACGCCTATAGTGCAATTTCAA GGTGAATCCAATTGTTTAAAGTGTTTTAGATA TAGGCTAAATGACAGACACAGACATTTATTT GATTTAATATCATCAACGTGGCACTGGGCCTC CTCAAAGGCACCACATAAACATGCCATTGTA ACTGTAACATATGATAGTGAGGAACAAAGGC AACAGTTTTTAGATGTTGTAAAAATACCCCCT ACCATTAGCCACAAACTGGGATTTATGTCACT GCACCTATTGTAA ATGGGAGCACCAAACATTGGGAAGTATGTTA TGGCAGCACAGTTATATGTTCTCCTGCATCTG TATCTAGCACTACACAAGAAGTATCCATTCCT GAATCTACTACATACACCCCCGCACAGACCTC CACCCTTGTGTCCTCAAGCACCAAGGAAGAC GCAGTGCAAACGCCGCCTAGGAAACGAGCA CGAGGAGTCCAACAGTCCCCTTGCAACGCCT TGTGTGTGGCCCACATTGGACCCGTGGACAG TGGAAACCACAACCTCATCACTAACAATCACG ACCAGCACCAAAGACGGAACAACAGTAACA GTTCAGCTACGCCTATAG ATGGAAGTGGTGCCTGTACAAATAGCTGCAG GAACAACCAGCACATTCATACTGCCTGTTATA ATTGCATTTGTTGTATGTTTTGTTAGCATCATA CTTATTGTATGGATATCTGAGTTTATTGTGTA CACATCTGTGCTAGTACTAACACTGCTTTTAT ATTTACTATTGTGGCTGCTATTAACAACCCCC TTGCAATTTTTCCTACTAACTCTACTTGTGTGT TACTGTCCCGCATTGTATATACACTACTATATT GTTACCACACAGCAATGA ATGATGCTAACATGTCAATTTAATGATGGAG ATACCTGGCTGGGTTTGTGGTTGTTATGTGCC TTTATTGTAGGGATGTTGGGGTTATTATTGAT GCACTATAGAGCTGTACAAGGGGATAAACAC ACCAAATGTAAGAAGTGTAACAAACACAACT GTAATGATGATTATGTAACTATGCATTATACT ACTGATGGTGATTATATATATATGAATTAG ATGGAAAGTGCAAATGCCTCCACGTCTGCAA CGACCATAGACCAGTTGTGCAAGACGTTTAA TCTATCTATGCATACGTTGCAAATTAATTGTG TGTTTTGCAAGAATGCACTGACCACAGCAGA GATTTATTCATATGCATATAAACACCTAAAGG TCCTGTTTCGAGGCGGCTATCCATATGCAGCC TGCGCGTGCTGCCTAGAATTTCATGGAAAAA TAAACCAATATAGACACTTTGATTATGCTGGA TATGCAACAACAGTTGAAGAAGAAACTAAAC AAGACATCTTAGACGTGCTAATTCGGTGCTAC ATGGAGGCTATTGCTAAAAGACTGGACGCTT GTCAGGAACAGCTGCTGGAACTGTACGAGG AAAACTCTACCGATCTGCATAAACATGTGCTG CACTGGAAGTGCATGCGCCATGAGTCCGTGC TGCTGTACAAGGCCAAACAGATGGGCCTGTC TCACATCGGGATGCAGGTGGTCCCCCCTCTG AAGGTGAGTGAAGCTAAAGGCCACAACGCA ATTGAGATGCAGATGCATCTGGAAAGCCTGC TGCGGACCGAGTACTCCATGGAACCATGGAC TCTGCAGGAGACCTCCTATGAAATGTGGCAG ACCCCACCCAAGCGATGCTTCAAGAAAAGGG GCAAGACAGTGGAGGTCAAATTTGACGGGT GTGCCAACAATACCATGGACTACGTGGTCTG GACAGATGTGTATGTCCAGGACAACGATACA TGGGTGAAGGTCCACTCTATGGTGGATGCTA AAGGGATCTACTATACATGTGGACAGTTCAA GACTTACTATGTGAATTTTGTCAAGGAGGCA GAAAAATACGGATCAACAAAACATTGGGAG GTGTGCTATGGCAGCACTGTCATCTGTTCCCC TGCATCTGTGAGCTCCACCACACAGGAGGTC AGTATTCCAGAATCAACTACCTATACCCCCGC CCAGACAAGCACTCTGGTGTCTAGTTCAACTA AGGAAGACGCCGTGCAGACCCCTCCAAGGA AACGAGCTCGAGGAGTGCAGCAGTCCCCTTG CAACGCCCTGTGTGTGGCTCACATCGGACCA GTCGACTCTGGCAACCATAATCTGATTACTAA CAATCACGATCAGCATCAGCGGAGAAACAAT AGTAATAGCTCCGCCACCCCCATTGTGCAGTT CCAGGGAGAGTCAAACTGCCTGAAGTGTTTT CGGTACAGACTGAATGACAGGCACCGCCATC TGTTCGATCTGATCTCTAGTACATGGCACTGG GCATCAAGCAAGGCCCCTCACAAACATGCTA TTGTGACCGTCACATATGACTCCGAGGAACA GAGACAGCAGTTCCTGGATGTGGTCAAGATC CCCCCTACTATTTCTCACAAACTGGGGTTTAT GAGTCTGCATCTGCTG ATGGGGGCTCCTAATATCGGAAAGTATGTCA TGGCCGCTCAGCTGTATGTCCTGCTGCATCTG TATCTGGCACTGCACAAGAAGTATCCCTTCCT GAACCTGCTGCACACTCCCCCTCATAGGCCAC CACCTCTGTGCCCACAGGCACCACGAAAGAC CCAGTGTAAACGGAGACTGGGCAACGAGCA CGAGGAATCTAATAGTCCTCTGGCTACACCAT GCGTGTGGCCCACACTGGACCCTTGGACTGT CGAAACCACAACTAGCTCCCTGACCATCACCA CATCCACAAAGGATGGGACTACCGTGACCGT CCAGCTGCGACTG ATGGAGGTCGTCCCCGTCCAGATTGCCGCCG GAACCACTTCAACTTTCATCCTGCCCGTCATC ATTGCTTTCGTCGTGTGTTTCGTGTCTATCATT CTGATCGTGTGGATTAGCGAGTTCATCGTCTA CACATCCGTGCTGGTCCTGACTCTGCTGCTGT ATCTGCTGCTGTGGCTGCTGCTGACCACACCC CTGCAGTTCTTTCTGCTGACCCTGCTGGTGTG CTACTGTCCTGCCCTGTATATCCACTACTATAT TGTCACTACCCAGCAG ATGATGCTGACTTGTCAGTTCAACGATGGCG ATACTTGGCTGGGGCTGTGGCTGCTGTGTGC TTTTATTGTCGGAATGCTGGGGCTGCTGCTG ATGCACTACCGGGCCGTGCAGGGCGACAAG CATACTAAATGCAAGAAATGTAACAAGCACA ACTGCAATGACGATTACGTCACCATGCATTAT ACCACAGACGGGGATTACATCTATATGAAT ATGGAGTCCGCTAACGCTTCTACTTCCGCAAC TACTATCGACCAGCTGTGTAAGACCTTCAACC TGTCAATGCATACCCTGCAGATTAACTGCGTG TTCTGTAAGAATGCTCTGACCACAGCAGAAA TCTACAGCTATGCCTACAAGCACCTGAAAGTC CTGTTTAGGGGCGGGTATCCCTACGCCGCTT GCGCTTGCTGTCTGGAGTTCCACGGAAAAAT TAACCAGTATCGCCATTTTGACTATGCAGGCT ACGCCACTACCGTGGAGGAAGAGACCAAGC AGGACATCCTGGATGTCCTGATTCGATGCTA MEAIAKRLDACQEQLLELYEENSTDL HKHVLHWKCMRHESVLLYKAKQM GLSHIGMQVVPPLKVSEAKGHNAIE MQMHLESLLRTEYSMEPWTLQETS YEMWQTPPKRCFKKRGKTVEVKFD GCANNTMDYVVWTDVYVQDNDT WVKVHSMVDAKGIYYTCGQFKTYY VNFVKEAEKYGSTKHWEVCYGSTVI CSPASVSSTTQEVSIPESTTYTPAQTS TLVSSSTKEDAVQTPPRKRARGVQQ SPCNALCVAHIGPVDSGNHNLITNN HDQHQRRNNSNSSATPIVQFQGES NCLKCFRYRLNDRHRHLFDLISSTWH WASSKAPHKHAIVTVTYDSEEQRQQ FLDVVKIPPTISHKLGFMSLHLL Page 2 of 48 % Nucleotide Change 25.5% MGAPNIGKYVMAAQLYVLLHLYLAL HKKYPFLNLLHTPPHRPPPLCPQAPR KTQCKRRLGNEHEESNSPLATPCVW PTLDPWTVETTTSSLTITTSTKDGTTV TVQLRL 23.6% MEVVPVQIAAGTTSTFILPVIIAFVVC FVSIILIVWISEFIVYTSVLVLTLLLYLLL WLLLTTPLQFFLLTLLVCYCPALYIHYY IVTTQQ 27.2% MMLTCQFNDGDTWLGLWLLCAFIV GMLGLLLMHYRAVQGDKHTKCKKC NKHNCNDDYVTMHYTTDGDYIYM N 22.4% MESANASTSATTIDQLCKTFNLSMH TLQINCVFCKNALTTAEIYSYAYKHLK VLFRGGYPYAACACCLEFHGKINQYR HFDYAGYATTVEEETKQDILDVLIRCY LCHKPLCEVEKVKHILTKARFIKLNCT WKGRCLHCWTTCMEDMLP 22.5% Gene HPV6 E7 HPV6 L1 PaVE DNA Sequence Optimized DNA Sequence CTGTGTCACAAACCGCTGTGTGAAGTAGAAA AGGTAAAACATATACTAACCAAGGCGCGGTT CATAAAGCTAAATTGTACGTGGAAGGGTCGC TGCCTACACTGCTGGACAACATGCATGGAAG ACATGTTACCCTAA CCTGTGTCACAAACCCCTGTGTGAAGTGGAG AAGGTCAAACATATCCTGACCAAGGCCCGGT TCATCAAGCTGAACTGCACATGGAAGGGGAG ATGCCTGCATTGTTGGACAACTTGTATGGAA GATATGCTGCCT ATGCATGGAAGACATGTTACCCTAAAGGATA TTGTATTAGACCTGCAACCTCCAGACCCTGTA GGGTTACATTGCTATGAGCAATTAGTAGACA GCTCAGAAGATGAGGTGGACGAAGTGGACG GACAAGATTCACAACCTTTAAAACAACATTTC CAAATAGTGACCTGTTGCTGTGGATGTGACA GCAACGTTCGACTGGTTGTGCAGTGTACAGA AACAGACATCAGAGAAGTGCAACAGCTTCTG TTGGGAACACTAAACATAGTGTGTCCCATCTG CGCACCGAAGACCTAA ATGTGGCGGCCTAGCGACAGCACAGTATATG TGCCTCCTCCTAACCCTGTATCCAAAGTTGTT GCCACGGATGCTTATGTTACTCGCACCAACAT ATTTTATCATGCCAGCAGTTCTAGACTTCTTG CAGTGGGACATCCTTATTTTTCCATAAAACGG GCTAACAAAACTGTTGTGCCAAAGGTGTCAG GATATCAATACAGGGTATTTAAGGTGGTGTT ACCAGATCCTAACAAATTTGCATTGCCTGACT CGTCTCTTTTCGATCCCACAACACAACGTTTA GTATGGGCATGCACAGGCCTAGAGGTGGGC AGGGGACAGCCATTAGGTGTGGGTGTAAGT GGACATCCTTTCCTAAATAAATATGATGATGT TGAAAATTCAGGGAGTGGTGGTAACCCTGGA CAGGATAACAGGGTTAATGTAGGTATGGATT ATAAACAAACACAATTATGCATGGTTGGATG TGCCCCCCCTTTGGGCGAGCATTGGGGTAAA GGTAAACAGTGTACTAATACACCTGTACAGG CTGGTGACTGCCCGCCCTTAGAACTTATTACC AGTGTTATACAGGATGGCGATATGGTTGACA CAGGCTTTGGTGCTATGAATTTTGCTGATTTG CAGACCAATAAATCAGATGTTCCTATTGACAT ATGTGGCACTACATGTAAATATCCAGATTATT TACAAATGGCTGCAGACCCATATGGTGATAG ATTATTTTTTTTTCTACGGAAGGAACAAATGT TTGCCAGACATTTTTTTAACAGGGCTGGCGA GGTGGGGGAACCTGTGCCTGATACACTTATA ATTAAGGGTAGTGGAAATCGCACGTCTGTAG GGAGTAGTATATATGTTAACACCCCGAGCGG CTCTTTGGTGTCCTCTGAGGCACAATTGTTTA ATAAGCCATATTGGCTACAAAAAGCCCAGGG ACATAACAATGGTATTTGTTGGGGTAATCAA CTGTTTGTTACTGTGGTAGATACCACACGCAG TACCAACATGACATTATGTGCATCCGTAACTA CATCTTCCACATACACCAATTCTGATTATAAA GAGTACATGCGTCATGTGGAAGAGTATGATT TACAATTTATTTTTCAATTATGTAGCATTACAT TGTCTGCTGAAGTAATGGCCTATATTCACACA ATGAATCCCTCTGTTTTGGAAGACTGGAACTT TGGGTTATCGCCTCCCCCAAATGGTACATTAG AAGATACCTATAGGTATGTGCAGTCACAGGC CATTACCTGTCAAAAGCCCACTCCTGAAAAG GAAAAGCCAGATCCCTATAAGAACCTTAGTTT TTGGGAGGTTAATTTAAAAGAAAAGTTTTCTA GTGAATTGGATCAGTATCCTTTGGGACGCAA GTTTTTGTTACAAAGTGGATATAGGGGACGG TCCTCTATTCGTACAGGTGTTAAGCGCCCTGC TGTTTCCAAAGCCTCTGCTGCCCCTAAACGTA AGCGCGCCAAAACTAAAAGGTAA ATGCACGGAAGACACGTCACCCTGAAAGATA TTGTCCTGGACCTGCAGCCTCCCGACCCTGTG GGCCTGCATTGCTATGAACAGCTGGTGGACA GCTCCGAGGACGAAGTGGATGAGGTCGACG GCCAGGATTCTCAGCCCCTGAAGCAGCACTT CCAGATCGTGACATGCTGTTGCGGGTGTGAC AGCAACGTCCGGCTGGTGGTCCAGTGCACCG AGACAGATATTAGAGAAGTGCAGCAGCTGCT GCTGGGCACTCTGAATATCGTCTGTCCCATTT GCGCCCCTAAAACC ATGTGGCGGCCTTCAGATTCAACTGTCTATGT GCCCCCTCCAAACCCCGTGTCAAAAGTCGTCG CTACCGATGCTTATGTCACCAGAACCAATATC TTTTACCACGCTAGCTCCTCTAGGCTGCTGGC AGTGGGCCATCCATATTTCTCAATTAAGCGCG CCAACAAGACAGTGGTCCCCAAGGTGTCTGG CTACCAGTATAGGGTCTTTAAGGTGGTCCTG CCTGACCCAAACAAATTTGCTCTGCCCGACAG TTCACTGTTCGATCCTACCACACAGCGGCTGG TGTGGGCATGCACTGGCCTGGAAGTCGGAA GAGGACAGCCACTGGGAGTGGGAGTCTCCG GACACCCCTTCCTGAATAAGTACGACGATGT GGAGAACAGCGGATCCGGAGGAAATCCAGG ACAGGACAACCGAGTGAATGTCGGCATGGAT TATAAACAGACCCAGCTGTGCATGGTGGGAT GTGCACCACCTCTGGGAGAACATTGGGGCAA GGGGAAACAGTGCACTAACACCCCTGTGCAG GCTGGAGATTGTCCACCCCTGGAGCTGATCA CCTCCGTGATTCAGGACGGCGATATGGTCGA CACAGGATTTGGCGCTATGAACTTCGCAGAT CTGCAGACAAATAAGAGCGACGTGCCTATCG ATATTTGCGGGACTACCTGTAAATACCCTGAC TATCTGCAGATGGCCGCTGACCCATACGGAG ATCGCCTGTTCTTTTTCCTGCGAAAGGAACAG ATGTTCGCCCGACACTTTTTCAATCGAGCTGG AGAAGTGGGAGAACCAGTCCCTGATACCCTG ATCATCAAGGGGAGTGGAAATAGGACATCA GTGGGGAGCTCCATCTACGTCAACACTCCTTC TGGAAGTCTGGTGTCTAGTGAGGCACAGCTG TTTAACAAGCCATATTGGCTGCAGAAAGCCC AGGGGCATAACAATGGAATTTGCTGGGGCA ATCAGCTGTTCGTGACCGTGGTCGACACAAC TCGAAGCACCAACATGACACTGTGTGCCTCC GTGACCACATCAAGCACATACACTAACTCCG ACTACAAGGAGTATATGCGCCACGTGGAGGA ATATGATCTGCAGTTTATCTTCCAGCTGTGCT CCATTACTCTGTCTGCCGAAGTGATGGCTTAC ATCCATACCATGAACCCATCTGTCCTGGAGGA CTGGAATTTTGGACTGAGTCCTCCACCCAACG GCACTCTGGAGGATACCTACAGATATGTGCA GAGTCAGGCAATTACATGTCAGAAGCCAACT CCCGAGAAGGAAAAACCTGACCCATATAAAA ACCTGTCTTTTTGGGAAGTGAATCTGAAGGA AAAATTCTCCTCTGAGCTGGATCAGTACCCCC TGGGCCGGAAGTTCCTGCTGCAGAGCGGATA TCGGGGCAGAAGTTCAATCAGAACAGGGGT GAAGAGGCCCGCAGTCTCAAAAGCCAGCGC AGCCCCTAAGAGGAAACGCGCTAAGACTAAA AGA Page 3 of 48 Protein Sequence % Nucleotide Change MHGRHVTLKDIVLDLQPPDPVGLHC YEQLVDSSEDEVDEVDGQDSQPLKQ HFQIVTCCCGCDSNVRLVVQCTETDI REVQQLLLGTLNIVCPICAPKT 22.9% MWRPSDSTVYVPPPNPVSKVVATD AYVTRTNIFYHASSSRLLAVGHPYFSI KRANKTVVPKVSGYQYRVFKVVLPD PNKFALPDSSLFDPTTQRLVWACTG LEVGRGQPLGVGVSGHPFLNKYDDV ENSGSGGNPGQDNRVNVGMDYKQ TQLCMVGCAPPLGEHWGKGKQCT NTPVQAGDCPPLELITSVIQDGDMV DTGFGAMNFADLQTNKSDVPIDICG TTCKYPDYLQMAADPYGDRLFFFLR KEQMFARHFFNRAGEVGEPVPDTLII KGSGNRTSVGSSIYVNTPSGSLVSSE AQLFNKPYWLQKAQGHNNGICWG NQLFVTVVDTTRSTNMTLCASVTTS STYTNSDYKEYMRHVEEYDLQFIFQL CSITLSAEVMAYIHTMNPSVLEDWN FGLSPPPNGTLEDTYRYVQSQAITCQ KPTPEKEKPDPYKNLSFWEVNLKEKF SSELDQYPLGRKFLLQSGYRGRSSIRT GVKRPAVSKASAAPKRKRAKTKR 25.2% Gene HPV6 L2 PaVE DNA Sequence Optimized DNA Sequence Protein Sequence ATGGCACATAGTAGGGCCCGACGACGCAAG CGTGCGTCAGCTACACAGCTATATCAAACAT GTAAACTCACTGGAACATGCCCCCCAGATGT AATTCCTAAGGTGGAGCACAACACCATTGCA GATCAAATATTAAAATGGGGAAGTTTGGGGG TGTTTTTTGGAGGGTTGGGTATAGGCACGGG TTCCGGCACTGGGGGTCGTACTGGCTATGTT CCCTTACAAACTTCTGCAAAACCTTCTATTACT AGTGGGCCTATGGCTCGTCCTCCTGTGGTGG TGGAGCCTGTGGCCCCTTCGGATCCATCTATT GTGTCTTTAATTGAAGAATCGGCAATCATTAA CGCAGGGGCGCCTGAAATTGTGCCCCCTGCA CACGGTGGGTTTACAATTACATCCTCTGAAAC AACTACCCCTGCAATATTGGATGTATCAGTTA CTAGTCACACTACTACTAGTATATTTAGAAAT CCTGTCTTTACAGAACCTTCTGTAACACAACC CCAACCACCCGTGGAGGCTAATGGACATATA TTAATTTCTGCACCCACTGTAACGTCACACCC TATAGAGGAAATTCCTTTAGATACTTTTGTGG TATCATCTAGTGATAGCGGTCCTACATCCAGT ACCCCTGTTCCTGGTACTGCACCTCGGCCTCG TGTGGGCCTATATAGTCGTGCATTGCACCAG GTGCAGGTTACAGACCCTGCATTTCTTTCCAC TCCTCAACGCTTAATTACATATGATAACCCTG TATATGAAGGGGAGGATGTTAGTGTACAATT TAGTCATGATTCTATACACAATGCACCTGATG AGGCTTTTATGGACATAATTCGTTTGCACAGA CCTGCCATTGCGTCCCGACGTGGCCTTGTGC GGTACAGTCGCATTGGACAACGGGGGTCTAT GCACACTCGCAGCGGAAAGCACATAGGGGC CCGCATTCATTATTTTTATGATATTTCACCTAT TGCACAGGCTGCAGAAGAAATAGAAATGCAC CCTCTTGTGGCTGCACAGGATGATACATTTGA TATTTATGCTGAATCTTTTGAACCTGGCATTA ACCCTACCCAACACCCTGTTACAAATATATCA GATACATATTTAACTTCCACACCTAATACAGT TACACAACCGTGGGGTAACACCACAGTTCCA TTGTCACTTCCTAATGACCTGTTTTTACAATCT GGCCCTGATATAACTTTTCCTACTGCACCTAT GGGAACACCCTTTAGTCCTGTAACTCCTGCTT TACCTACAGGCCCTGTTTTCATTACAGGTTCT GGATTTTATTTGCATCCTGCATGGTATTTTGC ACGTAAACGCCGTAAACGTATTCCCTTATTTT TTTCAGATGTGGCGGCCTAG ATGGCACACTCAAGGGCACGAAGAAGAAAG AGGGCATCCGCTACCCAGCTGTACCAGACCT GTAAACTGACCGGCACCTGTCCACCTGATGTC ATCCCTAAGGTGGAGCATAACACCATCGCCG ACCAGATTCTGAAATGGGGCAGTCTGGGGGT GTTCTTTGGCGGGCTGGGCATTGGGACTGGA TCAGGAACCGGAGGACGAACAGGATACGTG CCACTGCAGACTAGCGCTAAGCCCTCTATCAC CAGTGGACCTATGGCAAGACCCCCTGTGGTC GTGGAACCTGTCGCCCCATCAGATCCCAGCA TCGTGTCCCTGATTGAGGAAAGCGCTATCATT AATGCAGGAGCTCCAGAGATCGTGCCACCAG CACATGGGGGCTTCACCATTACCAGCTCCGA AACCACAACTCCTGCTATCCTGGACGTCTCTG TGACCAGTCACACCACAACTTCCATCTTCAGG AACCCTGTCTTTACTGAGCCATCTGTGACCCA GCCCCAGCCTCCAGTCGAAGCAAATGGACAT ATCCTGATTAGTGCCCCAACAGTGACTTCACA CCCTATCGAGGAAATTCCACTGGACACCTTTG TCGTGTCTAGTTCAGATTCCGGACCAACAAGC TCCACTCCAGTCCCTGGAACAGCACCACGACC ACGAGTGGGACTGTACTCCCGAGCTCTGCAT CAGGTCCAGGTGACCGATCCAGCATTCCTGT CTACCCCCCAGCGCCTGATTACATACGATAAC CCCGTGTATGAGGGGGAAGATGTCAGCGTG CAGTTTTCACACGACAGCATCCATAATGCTCC AGACGAGGCATTCATGGATATCATTAGACTG CACAGGCCCGCAATTGCCTCTCGGAGAGGCC TGGTGCGCTATAGTCGAATCGGACAGAGGG GCTCCATGCACACACGCTCTGGGAAACATAT CGGAGCCCGCATTCACTACTTTTATGACATCA GCCCCATTGCTCAGGCCGCTGAGGAAATTGA GATGCATCCTCTGGTGGCAGCCCAGGACGAT ACCTTCGATATCTACGCCGAGAGCTTTGAACC AGGCATTAACCCCACACAGCACCCTGTGACT AATATCAGCGACACCTATCTGACCTCCACACC TAACACTGTCACCCAGCCATGGGGGAATACC ACAGTGCCACTGTCACTGCCCAACGATCTGTT CCTGCAGAGCGGACCTGACATCACCTTTCCTA CAGCACCAATGGGCACACCCTTCAGTCCTGTC ACACCAGCCCTGCCCACTGGCCCTGTGTTCAT TACTGGGTCTGGATTTTACCTGCACCCTGCCT GGTATTTCGCTCGGAAGAGGCGCAAAAGAAT CCCACTGTTCTTTTCCGATGTGGCTGCA MAHSRARRRKRASATQLYQTCKLTG TCPPDVIPKVEHNTIADQILKWGSLG VFFGGLGIGTGSGTGGRTGYVPLQT SAKPSITSGPMARPPVVVEPVAPSDP SIVSLIEESAIINAGAPEIVPPAHGGFTI TSSETTTPAILDVSVTSHTTTSIFRNPV FTEPSVTQPQPPVEANGHILISAPTV TSHPIEEIPLDTFVVSSSDSGPTSSTPV PGTAPRPRVGLYSRALHQVQVTDPA FLSTPQRLITYDNPVYEGEDVSVQFS HDSIHNAPDEAFMDIIRLHRPAIASR RGLVRYSRIGQRGSMHTRSGKHIGA RIHYFYDISPIAQAAEEIEMHPLVAAQ DDTFDIYAESFEPGINPTQHPVTNIS DTYLTSTPNTVTQPWGNTTVPLSLP NDLFLQSGPDITFPTAPMGTPFSPVT PALPTGPVFITGSGFYLHPAWYFARK RRKRIPLFFSDVAA Page 4 of 48 % Nucleotide Change 26.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV11 E1 ATGGCGGACGATTCAGGTACAGAAAATGAG GGGTCGGGGTGTACAGGATGGTTTATGGTA GAAGCCATAGTAGAGCACACTACAGGTACAC AAATATCAGAAGATGAGGAAGAGGAGGTGG AGGACAGTGGGTATGACATGGTGGACTTTAT TGATGACAGGCATATTACACAAAATTCTGTG GAAGCACAGGCATTGTTTAATAGGCAGGAG GCGGATGCTCATTATGCGACTGTGCAGGACC TAAAACGAAAGTATTTAGGCAGTCCATATGT AAGTCCTATAAGCAATGTAGCTAATGCAGTA GAAAGTGAGATAAGTCCACGGTTAGACGCCA TTAAACTTACAACACAGCCAAAAAAGGTAAA GCGACGGCTGTTTGAAACACGGGAATTAACG GACAGTGGATATGGCTATTCTGAAGTGGAAG CTGCAACGCAGGTAGAGAAACATGGCGACCC GGAAAATGGGGGAGATGGTCAGGAAAGGG ACACAGGGAGGGACATAGAGGGTGAGGGG GTGGAACATAGAGAGGCGGAAGCAGTAGAC GACAGCACCCGAGAGCATGCAGACACATCAG GAATATTAGAATTACTAAAATGTAAGGATAT ACGATCTACATTACATGGTAAGTTTAAAGACT GCTTTGGGCTGTCATTTGTTGATTTAATTAGG CCATTTAAAAGTGATAGAACCACATGTGCCG ATTGGGTGGTTGCAGGATTTGGTATACATCA TAGCATAGCAGATGCATTTCAAAAGTTAATTG AGCCATTAAGTTTATATGCACATATACAATGG CTTACAAATGCATGGGGAATGGTACTATTAG TATTAATAAGGTTTAAAGTAAATAAGAGCAG ATGTACCGTGGCACGTACATTAGGTACGTTA TTAAATATACCTGAAAATCACATGTTAATTGA GCCTCCTAAAATACAAAGTGGCGTACGAGCC CTGTATTGGTTTAGGACAGGCATTTCAAATGC AAGTACAGTTATAGGGGAGGCGCCGGAATG GATAACGCGCCAGACCGTTATTGAACATAGT TTGGCTGACAGTCAATTTAAATTAACTGAAAT GGTGCAGTGGGCATATGATAATGATATTTGT GAAGAAAGTGAGATAGCATTTGAATATGCAC AGCGTGGAGACTTTGACTCCAATGCAAGGGC CTTTTTAAATAGTAATATGCAGGCTAAATATG TAAAAGATTGTGCAATTATGTGCAGACATTAT AAACATGCAGAAATGAAAAAGATGTCTATTA AACAATGGATTAAGTATAGGGGTACTAAAGT TGACAGTGTAGGTAACTGGAAGCCAATTGTG CAGTTTCTAAGACATCAAAACATAGAATTTAT TCCATTTTTAAGCAAACTAAAATTATGGCTGC ACGGAACGCCCAAAAAAAATTGTATAGCCAT TGTAGGGCCACCTGACACTGGGAAGTCGTGC TTTTGCATGAGTTTAATTAAGTTTTTGGGGGG AACAGTTATTAGTTATGTTAATTCCTGCAGCC ATTTCTGGCTACAGCCACTAACGGATGCAAA AGTGGCATTATTGGATGATGCCACACAACCA TGTTGGACATATATGGATACATATATGAGAA ACCTATTAGATGGTAATCCTATGAGCATAGAT AGAAAACATAGAGCATTAACATTAATTAAGT GTCCACCGCTACTGGTTACATCAAATATAGAC ATTAGCAAAGAGGAGAAATACAAATATTTAC ATAGTAGAGTTACCACATTTACATTTCCAAAT CCATTCCCCTTTGACAGAAATGGGAATGCAG TATATGAACTATCAGATGCAAACTGGAAATG TTTCTTTGAAAGACTGTCGTCCAGCCTAGACA TTGAGGATTCAGAGGACGAGGAAGATGGAA GCAATAGCCAAGCGTTTAGATGCGTGCCAGG ATCAGTTGTTAGAACTTTATGA ATGGCAGATGACAGCGGGACCGAGAATGAG GGGAGCGGATGCACTGGGTGGTTTATGGTG GAGGCAATCGTGGAACACACTACTGGCACCC AGATCAGCGAGGACGAGGAAGAGGAAGTG GAAGATTCCGGATACGACATGGTCGATTTTA TCGACGATCGGCACATTACTCAGAACAGCGT GGAGGCACAGGCCCTGTTCAATAGACAGGA AGCTGACGCACATTATGCCACCGTGCAGGAT CTGAAGAGGAAATACCTGGGCAGCCCCTATG TCTCTCCTATCAGTAACGTGGCCAATGCTGTC GAGTCAGAAATCAGCCCAAGACTGGACGCCA TTAAGCTGACCACACAGCCCAAGAAAGTGAA ACGGAGACTGTTTGAGACAAGGGAACTGACT GATAGCGGGTACGGATATTCCGAGGTGGAA GCCGCTACCCAGGTCGAGAAGCACGGCGACC CAGAAAACGGAGGGGATGGACAGGAGCGA GACACAGGGCGGGATATCGAGGGCGAAGGG GTGGAGCACAGAGAGGCAGAAGCCGTCGAC GATTCCACTAGGGAGCATGCCGACACCTCTG GGATCCTGGAACTGCTGAAGTGCAAAGATAT TCGCTCCACCCTGCATGGAAAGTTCAAAGACT GTTTTGGCCTGTCTTTCGTGGATCTGATCCGC CCATTCAAGAGTGACCGAACTACCTGCGCCG ATTGGGTGGTCGCTGGATTTGGCATCCACCA TTCAATTGCTGACGCATTCCAGAAACTGATCG AGCCCCTGAGCCTGTACGCACACATTCAGTG GCTGACAAACGCCTGGGGCATGGTGCTGCTG GTCCTGATCCGCTTTAAGGTGAACAAGTCTA GATGTACTGTCGCCAGGACCCTGGGGACACT GCTGAACATTCCTGAGAATCATATGCTGATCG AACCCCCTAAGATTCAGAGTGGAGTGCGGGC TCTGTATTGGTTCAGAACAGGCATCTCCAACG CATCTACTGTGATTGGGGAGGCCCCAGAATG GATCACTCGGCAGACCGTCATTGAGCACAGT CTGGCTGACTCACAGTTTAAGCTGACCGAGA TGGTGCAGTGGGCATACGACAACGATATCTG CGAGGAAAGCGAGATTGCTTTCGAATATGCA CAGAGGGGCGACTTTGATAGTAATGCCCGCG CTTTCCTGAACTCAAATATGCAGGCTAAGTAC GTGAAAGACTGCGCAATCATGTGTAGGCACT ATAAGCATGCCGAGATGAAGAAAATGTCCAT CAAGCAGTGGATCAAGTACCGCGGGACTAA GGTGGATTCTGTCGGAAACTGGAAACCCATT GTGCAGTTTCTGCGGCACCAGAATATCGAGT TCATTCCTTTTCTGTCCAAGCTGAAACTGTGG CTGCATGGCACACCAAAGAAAAACTGCATCG CCATTGTGGGGCCACCCGACACTGGAAAGTC TTGCTTTTGTATGAGTCTGATCAAATTCCTGG GAGGCACAGTGATTTCTTATGTCAATAGTTGC TCACACTTCTGGCTGCAGCCCCTGACTGACGC AAAGGTGGCCCTGCTGGACGATGCAACCCAG CCTTGTTGGACCTACATGGATACATATATGAG AAACCTGCTGGACGGGAATCCCATGAGCATC GATAGGAAGCACCGCGCTCTGACCCTGATCA AGTGTCCTCCACTGCTGGTGACATCAAACATC GATATTAGCAAGGAGGAAAAGTACAAATATC TGCATAGCCGCGTGACAACTTTCACCTTTCCC AACCCTTTCCCATTTGACCGAAACGGCAATGC CGTCTACGAGCTGTCCGATGCTAATTGGAAA TGCTTCTTTGAAAGGCTGAGCTCCTCTCTGGA CATCGAGGATAGTGAAGACGAGGAAGATGG AAGCAATTCCCAGGCCTTCCGATGTGTGCCT GGCTCAGTGGTCCGGACACTG MADDSGTENEGSGCTGWFMVEAI VEHTTGTQISEDEEEEVEDSGYDMV DFIDDRHITQNSVEAQALFNRQEAD AHYATVQDLKRKYLGSPYVSPISNVA NAVESEISPRLDAIKLTTQPKKVKRRL FETRELTDSGYGYSEVEAATQVEKHG DPENGGDGQERDTGRDIEGEGVEH REAEAVDDSTREHADTSGILELLKCK DIRSTLHGKFKDCFGLSFVDLIRPFKS DRTTCADWVVAGFGIHHSIADAFQK LIEPLSLYAHIQWLTNAWGMVLLVLI RFKVNKSRCTVARTLGTLLNIPENHM LIEPPKIQSGVRALYWFRTGISNASTV IGEAPEWITRQTVIEHSLADSQFKLTE MVQWAYDNDICEESEIAFEYAQRG DFDSNARAFLNSNMQAKYVKDCAI MCRHYKHAEMKKMSIKQWIKYRGT KVDSVGNWKPIVQFLRHQNIEFIPFL SKLKLWLHGTPKKNCIAIVGPPDTGK SCFCMSLIKFLGGTVISYVNSCSHFW LQPLTDAKVALLDDATQPCWTYMD TYMRNLLDGNPMSIDRKHRALTLIK CPPLLVTSNIDISKEEKYKYLHSRVTTF TFPNPFPFDRNGNAVYELSDANWK CFFERLSSSLDIEDSEDEEDGSNSQAF RCVPGSVVRTL Page 5 of 48 % Nucleotide Change 25.7% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV11 E2 ATGGAAGCAATAGCCAAGCGTTTAGATGCGT GCCAGGATCAGTTGTTAGAACTTTATGAAGA AAACAGTATTGATATACACAAACACATTATGC ATTGGAAATGCATACGATTGGAAAGTGTATT ACTACACAAAGCAAAACAAATGGGCCTGAGC CACATCGGGTTACAAGTAGTACCACCATTAAC TGTGTCAGAGACTAAAGGACATAATGCTATT GAAATGCAAATGCATTTAGAATCCTTAGCAA AAACTCAGTATGGTGTGGAACCTTGGACATT ACAGGACACCAGTTATGAAATGTGGCTAACA CCACCCAAACGGTGCTTTAAAAAACAGGGAA ATACTGTGGAGGTAAAATTTGATGGCTGTGA AGACAATGTAATGGAGTATGTGGTATGGACA CATATATACCTGCAGGACAACGACTCATGGG TAAAAGTAACTAGTTCCGTAGATGCCAAGGG CATATATTATACATGTGGACAATTTAAAACAT ATTATGTAAATTTTAATAAAGAGGCACAAAA GTATGGTAGTACCAATCATTGGGAAGTATGT TATGGCAGCACAGTTATATGTTCTCCTGCATC TGTATCTAGCACTGTACGAGAAGTATCCATTG CTGAACCTACTACATACACCCCCGCACAGACC ACCGCCCCTACAGTGTCCGCCTGCACCACGG AAGACGGCGTGTCGGCGCCGCCTAGGAAGC GAGCACGTGGACCGTCCACTAACAACACCCT GTGTGTGGCCAACATCAGATCCGTGGACAGT ACAATCAACAACATCGTCACTGACAATTACAA CAAGCACCAAAGAAGGAACAACTGTCACAGT GCAGCTACGCCTATAGTGCAACTGCAAGGTG ATTCCAATTGTTTAAAATGTTTTAGATATAGA CTGAATGACAAATATAAACATTTGTTTGAATT AGCATCTTCAACGTGGCATTGGGCCTCACCT GAGGCACCACATAAAAATGCAATTGTAACAT TAACATATAGCAGTGAGGAACAACGTCAGCA ATTTTTAAACAGTGTAAAAATACCACCCACCA TTAGGCATAAGGTGGGGTTTATGTCATTACA TTTATTGTAA ATGGTAGTACCAATCATTGGGAAGTATGTTA TGGCAGCACAGTTATATGTTCTCCTGCATCTG TATCTAGCACTGTACGAGAAGTATCCATTGCT GAACCTACTACATACACCCCCGCACAGACCAC CGCCCCTACAGTGTCCGCCTGCACCACGGAA GACGGCGTGTCGGCGCCGCCTAGGAAGCGA GCACGTGGACCGTCCACTAACAACACCCTGT GTGTGGCCAACATCAGATCCGTGGACAGTAC AATCAACAACATCGTCACTGACAATTACAACA AGCACCAAAGAAGGAACAACTGTCACAGTGC AGCTACGCCTTAG ATGGAGGTAGTGCCTGTACAAATTGCTGCAG CAACAACTACAACATTGATATTGCCTGTTGTT ATTGCATTTGCAGTATGTATTCTTAGTATTGT ACTTATAATATTAATATCTGATTTTGTAGTATA TACATCTGTGCTGGTACTAACACTTCTTTTATA TTTGCTTTTGTGGCTTTTATTAACAACCCCTTT GCAATTCTTTTTACTAACACTGTGTGTGTGCT ATTTTCCTGCCTTTTATATACACATATACATTG TGCAAACGCAACAATAA ATGGTGATGTTAACCTGTCACTTAAATGATG GTGATACATGGTTGTTTCTGTGGTTGTTTACT GCATTTGTTGTAGCTGTACTTGGATTGTTGTT ACTACATTACAGGGCTGTACATGGTACTGAA AAAACTAAATGTGCTAAGTGTAAATCAAACC GCAATACTACTGTGGATTATGTGTATATGTCA CATGGTGATAATGGAGATTATGTGTACATGA ACTAG ATGGAAAGTAAAGATGCCTCCACGTCTGCAA CATCTATAGACCAGTTGTGCAAGACGTTTAAT CTTTCTTTGCACACTCTGCAAATTCAGTGCGT GTTTTGCAGGAATGCACTGACCACCGCAGAG ATATATGCATATGCCTATAAGAACCTAAAGGT TGTGTGGCGAGACAACTTTCCCTTTGCAGCGT GTGCCTGTTGCTTAGAACTGCAAGGGAAAAT TAACCAATATAGACACTTTAATTATGCTGCAT ATGCACCTACAGTAGAAGAAGAAACCAATGA ATGGAAGCAATCGCAAAGAGACTGGATGCCT GTCAGGATCAGCTGCTGGAACTGTATGAGGA AAATAGTATCGACATTCACAAACATATCATGC ACTGGAAGTGCATTCGGCTGGAGTCCGTGCT GCTGCACAAGGCCAAACAGATGGGCCTGTCT CATATCGGGCTGCAGGTGGTCCCCCCTCTGA CAGTGTCTGAAACTAAGGGGCACAACGCAAT TGAGATGCAGATGCATCTGGAAAGTCTGGCC AAAACCCAGTACGGAGTGGAGCCTTGGACCC TGCAGGACACATCTTATGAAATGTGGCTGAC ACCACCCAAGCGGTGCTTCAAGAAACAGGGG AACACTGTGGAGGTCAAATTTGACGGATGTG AGGATAATGTGATGGAATACGTGGTCTGGAC CCACATCTATCTGCAGGACAACGATAGCTGG GTGAAGGTCACAAGCTCCGTGGATGCAAAAG GAATCTACTATACTTGCGGCCAGTTCAAGACC TACTATGTCAACTTTAATAAGGAGGCTCAGA AATACGGCTCAACTAATCATTGGGAAGTGTG CTATGGGAGCACCGTCATCTGTAGTCCAGCTT CAGTGTCTAGTACCGTGCGAGAGGTCAGCAT TGCAGAACCTACCACATACACCCCAGCACAG ACTACCGCCCCCACAGTGTCTGCCTGCACAAC TGAGGACGGAGTCAGTGCCCCTCCAAGGAAA CGCGCTCGAGGCCCTTCCACTAACAATACCCT GTGTGTGGCCAATATCAGGAGCGTCGACTCC ACAATCAACAACATCGTGACTGATAACTACA ATAAGCACCAGCGGAGAAACAATTGTCATAG TGCCGCTACTCCCATTGTGCAGCTGCAGGGC GACTCAAACTGCCTGAAATGTTTCCGGTACA GACTGAATGATAAGTATAAACACCTGTTTGA GCTGGCCTCAAGCACATGGCACTGGGCTTCC CCAGAAGCACCCCATAAGAACGCTATCGTGA CCCTGACATACTCCTCTGAGGAACAGAGGCA GCAGTTCCTGAATAGCGTGAAGATCCCCCCT ACCATTCGCCACAAAGTCGGGTTTATGTCCCT GCATCTGCTG ATGGTCGTCCCTATTATTGGAAAGTATGTGAT GGCCGCTCAGCTGTATGTCCTGCTGCACCTGT ATCTGGCTCTGTATGAGAAGTATCCACTGCTG AACCTGCTGCACACCCCACCTCATCGACCACC ACCTCTGCAGTGCCCACCAGCACCACGAAAG ACAGCTTGTCGGAGAAGGCTGGGCAGCGAG CACGTGGACAGACCTCTGACCACACCATGCG TCTGGCCCACTTCTGATCCTTGGACCGTGCAG AGTACTACCAGCTCCCTGACAATCACAACTTC TACTAAAGAAGGGACCACAGTGACCGTCCAG CTGCGGCTG ATGGAGGTCGTGCCAGTCCAGATTGCTGCCG CCACCACCACTACCCTGATCCTGCCAGTCGTC ATTGCCTTCGCTGTGTGTATCCTGTCTATTGT GCTGATCATTCTGATCAGCGACTTCGTGGTCT ACACTTCCGTGCTGGTCCTGACCCTGCTGCTG TATCTGCTGCTGTGGCTGCTGCTGACCACACC CCTGCAGTTCTTTCTGCTGACACTGTGCGTGT GTTACTTCCCTGCCTTTTACATCCACATCTACA TCGTCCAGACCCAGCAG ATGGTCATGCTGACTTGTCACCTGAACGATG GAGATACCTGGCTGTTTCTGTGGCTGTTTACC GCCTTTGTGGTCGCAGTGCTGGGCCTGCTGC TGCTGCACTACCGGGCCGTGCATGGCACTGA GAAGACCAAATGCGCTAAGTGTAAAAGCAAC AGAAATACCACAGTGGACTACGTCTATATGT CCCACGGCGACAACGGGGATTACGTCTATAT GAAT ATGGAGAGCAAGGACGCCTCAACATCCGCAA CAAGCATCGACCAGCTGTGTAAAACTTTCAAC CTGTCACTGCATACCCTGCAGATTCAGTGCGT GTTCTGTAGGAACGCCCTGACCACAGCTGAA ATCTACGCTTATGCATACAAGAACCTGAAAGT GGTCTGGCGCGACAATTTCCCATTTGCCGCTT GCGCATGCTGTCTGGAACTGCAGGGCAAGAT TAACCAGTATCGACACTTCAATTATGCAGCCT ACGCTCCCACAGTGGAGGAAGAGACCAATG MEAIAKRLDACQDQLLELYEENSIDI HKHIMHWKCIRLESVLLHKAKQMGL SHIGLQVVPPLTVSETKGHNAIEMQ MHLESLAKTQYGVEPWTLQDTSYE MWLTPPKRCFKKQGNTVEVKFDGC EDNVMEYVVWTHIYLQDNDSWVK VTSSVDAKGIYYTCGQFKTYYVNFNK EAQKYGSTNHWEVCYGSTVICSPAS VSSTVREVSIAEPTTYTPAQTTAPTVS ACTTEDGVSAPPRKRARGPSTNNTL CVANIRSVDSTINNIVTDNYNKHQRR NNCHSAATPIVQLQGDSNCLKCFRY RLNDKYKHLFELASSTWHWASPEAP HKNAIVTLTYSSEEQRQQFLNSVKIPP TIRHKVGFMSLHLL HPV11 E4 HPV11 E5A HPV11 E5B HPV11 E6 Page 6 of 48 % Nucleotide Change 24.1% MVVPIIGKYVMAAQLYVLLHLYLALY EKYPLLNLLHTPPHRPPPLQCPPAPR KTACRRRLGSEHVDRPLTTPCVWPT SDPWTVQSTTSSLTITTSTKEGTTVTV QLRL 26.1% MEVVPVQIAAATTTTLILPVVIAFAVC ILSIVLIILISDFVVYTSVLVLTLLLYLLL WLLLTTPLQFFLLTLCVCYFPAFYIHIY IVQTQQ 30.4% MVMLTCHLNDGDTWLFLWLFTAFV VAVLGLLLLHYRAVHGTEKTKCAKCK SNRNTTVDYVYMSHGDNGDYVYM N 24.9% MESKDASTSATSIDQLCKTFNLSLHTL QIQCVFCRNALTTAEIYAYAYKNLKV VWRDNFPFAACACCLELQGKINQYR HFNYAAYAPTVEEETNEDILKVLIRCY LCHKPLCEIEKLKHILGKARFIKLNNQ WKGRCLHCWTTCMEDLLP 24.8% Gene HPV11 E7 HPV11 L1 PaVE DNA Sequence Optimized DNA Sequence AGATATTTTAAAAGTGTTAATTCGTTGTTACC TGTGTCACAAGCCGTTGTGTGAAATAGAAAA ACTAAAGCACATATTGGGAAAGGCACGCTTC ATAAAACTAAATAACCAGTGGAAGGGTCGTT GCTTACACTGCTGGACAACATGCATGGAAGA CTTGTTACCCTAA AGGATATCCTGAAGGTCCTGATTAGATGCTA CCTGTGTCACAAACCTCTGTGCGAAATCGAG AAGCTGAAACATATTCTGGGCAAGGCCCGGT TTATCAAACTGAACAATCAGTGGAAAGGGAG ATGCCTGCATTGTTGGACTACCTGTATGGAG GACCTGCTGCCC ATGCATGGAAGACTTGTTACCCTAAAGGATA TAGTACTAGACCTGCAGCCTCCTGACCCTGTA GGGTTACATTGCTATGAGCAATTAGAAGACA GCTCAGAAGATGAGGTGGACAAGGTGGACA AACAAGACGCACAACCTTTAACACAACATTAC CAAATACTGACCTGTTGCTGTGGATGTGACA GCAACGTCCGACTGGTTGTGGAGTGCACAGA CGGAGACATCAGACAACTACAAGACCTTTTG CTGGGCACACTAAATATTGTGTGTCCCATCTG CGCACCAAAACCATAA ATGTGGCGGCCTAGCGACAGCACAGTATATG TGCCTCCTCCCAACCCTGTATCCAAGGTTGTT GCCACGGATGCGTATGTTAAACGCACCAACA TATTTTATCATGCCAGCAGTTCTAGACTCCTT GCTGTGGGACATCCATATTACTCTATCAAAAA AGTTAACAAAACAGTTGTACCAAAGGTGTCT GGATATCAATATAGAGTGTTTAAGGTAGTGT TGCCAGATCCTAACAAGTTTGCATTACCTGAT TCATCCCTGTTTGACCCCACTACACAGCGTTT AGTATGGGCGTGCACAGGGTTGGAGGTAGG CAGGGGTCAACCTTTAGGCGTTGGTGTTAGT GGGCATCCATTGCTAAACAAATATGATGATG TAGAAAATAGTGGTGGGTATGGTGGTAATCC TGGTCAGGATAATAGGGTTAATGTAGGTATG GATTATAAACAAACCCAGCTATGTATGGTGG GCTGTGCTCCACCGTTAGGTGAACATTGGGG TAAGGGTACACAATGTTCAAATACCTCTGTAC AAAATGGTGACTGCCCCCCGTTGGAACTTATT ACCAGTGTTATACAGGATGGGGACATGGTTG ATACAGGCTTTGGTGCTATGAATTTTGCAGAC TTACAAACCAATAAATCGGATGTTCCCCTTGA TATTTGTGGAACTGTCTGCAAATATCCTGATT ATTTGCAAATGGCTGCAGACCCTTATGGTGA TAGGTTGTTTTTTTATTTGCGAAAGGAACAAA TGTTTGCTAGACACTTTTTTAATAGGGCCGGT ACTGTGGGGGAACCTGTGCCTGATGACCTGT TGGTAAAAGGGGGTAATAACAGATCATCTGT AGCTAGTAGTATTTATGTACATACACCTAGTG GCTCATTGGTGTCTTCAGAGGCTCAATTATTT AATAAACCATATTGGCTTCAAAAGGCTCAGG GACATAACAATGGTATTTGCTGGGGAAACCA CTTGTTTGTTACTGTGGTAGATACCACACGCA GTACAAATATGACACTATGTGCATCTGTGTCT AAATCTGCTACATACACTAATTCAGATTATAA GGAATACATGCGCCATGTGGAGGAGTTTGAT TTACAGTTTATTTTTCAATTGTGTAGCATTACA TTATCTGCAGAAGTCATGGCCTATATACACAC AATGAATCCTTCTGTTTTGGAGGACTGGAACT TTGGTTTATCGCCTCCACCAAATGGTACACTG GAGGATACTTATAGATATGTACAGTCACAGG CCATTACCTGTCAGAAACCCACACCTGAAAAA GAAAAACAGGATCCCTATAAGGATATGAGTT TTTGGGAGGTTAACTTAAAAGAAAAGTTTTC AAGTGAATTAGATCAGTTTCCCCTTGGACGTA AGTTTTTATTGCAAAGTGGATATCGAGGACG GACGTCTGCTCGTACAGGTATAAAGCGCCCA GCTGTGTCTAAGCCCTCTACAGCCCCCAAACG AAAACGTACCAAAACCAAAAAGTAA ATGCACGGAAGACTGGTGACACTGAAAGAC ATCGTCCTGGATCTGCAGCCCCCTGACCCCGT CGGACTGCACTGCTATGAACAGCTGGAGGAC AGCTCCGAGGACGAAGTGGATAAGGTCGAC AAACAGGATGCCCAGCCACTGACCCAGCACT ACCAGATCCTGACATGCTGTTGCGGCTGTGA CTCTAACGTGCGGCTGGTGGTCGAATGCACT GACGGCGATATTAGACAGCTGCAGGATCTGC TGCTGGGGACCCTGAATATCGTCTGTCCCATT TGCGCTCCCAAGCCT ATGTGGCGGCCTTCTGATTCTACAGTCTATGT GCCTCCTCCAAACCCCGTGAGCAAAGTCGTC GCTACCGATGCCTACGTGAAAAGAACCAACA TCTTCTACCACGCAAGCTCCTCTCGACTGCTG GCCGTGGGCCATCCCTACTACAGTATTAAGA AAGTCAACAAGACAGTGGTCCCTAAAGTGTC AGGCTACCAGTATCGCGTCTTTAAGGTGGTC CTGCCTGACCCAAACAAGTTCGCTCTGCCCGA CAGTTCACTGTTTGATCCTACCACACAGCGAC TGGTGTGGGCATGCACCGGACTGGAAGTCG GAAGAGGACAGCCACTGGGAGTGGGCGTCT CTGGACACCCACTGCTGAACAAGTACGACGA TGTGGAGAATAGTGGAGGATATGGAGGAAA CCCAGGACAGGACAACAGGGTGAATGTCGG AATGGATTACAAGCAGACACAGCTGTGCATG GTGGGATGTGCACCACCTCTGGGAGAACATT GGGGGAAAGGAACTCAGTGCAGTAACACCT CAGTGCAGAATGGAGACTGTCCACCCCTGGA GCTGATCACCAGCGTGATTCAGGACGGCGAT ATGGTCGACACAGGCTTCGGGGCAATGAATT TTGCCGATCTGCAGACAAACAAGTCCGACGT GCCTCTGGATATCTGCGGGACTGTCTGTAAA TACCCTGATTATCTGCAGATGGCCGCTGACCC ATACGGAGATCGCCTGTTCTTTTATCTGCGAA AGGAACAGATGTTCGCTCGACACTTCTTTAAC CGAGCAGGAACTGTGGGAGAGCCAGTCCCT GACGATCTGCTGGTGAAAGGGGGAAACAAT CGCAGCTCCGTGGCCTCTAGTATCTACGTCCA TACACCAAGTGGCTCACTGGTGTCAAGCGAG GCACAGCTGTTCAATAAGCCCTATTGGCTGCA GAAAGCCCAGGGCCACAACAATGGGATTTGC TGGGGAAACCATCTGTTTGTGACCGTGGTCG ACACTACCAGGTCTACTAATATGACCCTGTGT GCCAGCGTGTCCAAGTCTGCTACATACACTAA CTCCGACTACAAAGAATATATGCGCCACGTG GAGGAATTCGATCTGCAGTTCATCTTTCAGCT GTGCTCTATTACTCTGAGTGCTGAAGTGATG GCATATATCCATACCATGAATCCCTCCGTCCT GGAGGACTGGAACTTTGGACTGTCTCCTCCA CCCAATGGCACACTGGAGGATACTTACAGAT ATGTGCAGAGCCAGGCCATTACATGTCAGAA GCCAACTCCCGAGAAGGAAAAACAGGACCCT TACAAAGATATGTCCTTCTGGGAAGTGAATCT GAAGGAAAAATTTTCCTCTGAGCTGGATCAG TTCCCACTGGGCAGAAAGTTTCTGCTGCAGTC AGGGTATCGGGGAAGAACCAGCGCTAGAAC AGGGATTAAGAGGCCCGCCGTGAGCAAACCT TCCACCGCTCCAAAGAGGAAACGCACCAAGA CAAAGAAA Page 7 of 48 Protein Sequence % Nucleotide Change MHGRLVTLKDIVLDLQPPDPVGLHC YEQLEDSSEDEVDKVDKQDAQPLTQ HYQILTCCCGCDSNVRLVVECTDGDI RQLQDLLLGTLNIVCPICAPKP 25.9% MWRPSDSTVYVPPPNPVSKVVATD AYVKRTNIFYHASSSRLLAVGHPYYSI KKVNKTVVPKVSGYQYRVFKVVLPD PNKFALPDSSLFDPTTQRLVWACTG LEVGRGQPLGVGVSGHPLLNKYDDV ENSGGYGGNPGQDNRVNVGMDYK QTQLCMVGCAPPLGEHWGKGTQC SNTSVQNGDCPPLELITSVIQDGDM VDTGFGAMNFADLQTNKSDVPLDIC GTVCKYPDYLQMAADPYGDRLFFYL RKEQMFARHFFNRAGTVGEPVPDD LLVKGGNNRSSVASSIYVHTPSGSLV SSEAQLFNKPYWLQKAQGHNNGIC WGNHLFVTVVDTTRSTNMTLCASV SKSATYTNSDYKEYMRHVEEFDLQFI FQLCSITLSAEVMAYIHTMNPSVLED WNFGLSPPPNGTLEDTYRYVQSQAI TCQKPTPEKEKQDPYKDMSFWEVN LKEKFSSELDQFPLGRKFLLQSGYRGR TSARTGIKRPAVSKPSTAPKRKRTKTK K 25.7% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV11 L2 ATGAAACCTAGGGCACGCAGACGTAAACGTG CGTCAGCCACACAACTATATCAAACATGCAA GGCCACTGGTACATGTCCCCCAGATGTAATTC CTAAAGTTGAACATACTACTATTGCAGATCAA ATATTAAAATGGGGAAGCTTAGGGGTTTTTT TTGGTGGGTTAGGTATTGGTACAGGGGCTGG TAGTGGCGGTCGTGCAGGGTATATACCCTTG GGAAGCTCTCCCAAGCCTGCTATTACTGGGG GGCCAGCAGCACGTCCGCCAGTGCTTGTGGA GCCTGTTGCCCCTTCCGATCCCTCCATTGTGT CCTTAATTGAGGAGTCTGCTATTATTAATGCT GGTGCACCTGAGGTGGTACCCCCTACACAGG GTGGCTTTACTATAACATCATCTGAATCGACT ACACCTGCTATTTTAGATGTGTCTGTTACCAA TCACACTACCACTAGTGTGTTTCAAAATCCCC TGTTTACAGAACCGTCTGTAATACAGCCCCAA CCACCTGTGGAGGCCAGTGGTCACATACTTA TATCTGCCCCAACAATAACATCCCAACATGTA GAAGACATTCCACTAGACACTTTTGTTGTATC CTCTAGTGATAGTGGACCTACATCCAGTACTC CTCTTCCTCGTGCTTTTCCTCGGCCTCGGGTG GGTTTGTATAGTCGTGCCTTACAGCAGGTAC AGGTTACGGACCCCGCGTTTTTGTCCACGCCA CAGCGATTGGTAACTTATGACAACCCTGTCTA TGAAGGAGAAGATGTAAGTTTACAATTTACC CATGAGTCTATCCACAATGCACCTGATGAAG CATTTATGGATATTATTAGACTACATAGACCA GCTATAACGTCCAGACGGGGTCTTGTGCGTT TTAGTCGCATTGGGCAACGGGGGTCCATGTA CACACGCAGTGGACAACATATAGGTGCCCGC ATACATTATTTTCAGGACATTTCACCAGTTAC ACAAGCTGCAGAGGAAATAGAACTGCACCCT CTAGTGGCTGCAGAAAATGACACGTTTGATA TTTATGCTGAACCATTTGACCCTATCCCTGAC CCTGTCCAACATTCTGTTACACAGTCTTATCTT ACCTCCACACCTAATACCCTTTCACAATCGTG GGGTAATACCACAGTCCCATTGTCAATCCCTA GTGACTGGTTTGTGCAGTCTGGGCCTGACAT AACTTTTCCTACTGCATCTATGGGAACACCCT TTAGTCCTGTAACTCCTGCTTTACCTACAGGC CCTGTTTTTATTACAGGTTCTGACTTCTATTTG CATCCTACATGGTACTTTGCACGCAGACGCCG TAAACGTATTCCCTTATTTTTTACAGATGTGG CGGCCTAG ATGAAACCAAGAGCAAGAAGAAGAAAAAGA GCATCCGCAACTCAGCTGTATCAGACCTGTAA AGCCACTGGAACCTGTCCCCCCGACGTGATC CCCAAGGTCGAGCACACCACAATCGCCGACC AGATTCTGAAATGGGGCTCTCTGGGGGTGTT CTTTGGCGGGCTGGGAATCGGAACCGGAGC AGGAAGCGGAGGACGAGCTGGATACATCCC ACTGGGAAGCTCCCCAAAGCCTGCTATTACA GGAGGACCTGCAGCTAGACCACCTGTGCTGG TCGAACCCGTCGCACCTTCCGACCCATCTATC GTGAGTCTGATTGAGGAAAGCGCCATCATTA ACGCAGGAGCACCAGAGGTGGTCCCACCAAC CCAGGGCGGGTTTACCATCACATCTAGTGAA TCCACTACCCCTGCCATTCTGGATGTCAGTGT CACCAACCATACAACTACCTCAGTGTTCCAGA ATCCTCTGTTTACAGAGCCATCCGTCATCCAG CCCCAGCCTCCAGTGGAAGCATCCGGCCACA TCCTGATTTCTGCCCCAACTATCACCAGTCAG CATGTGGAGGACATTCCCCTGGATACCTTTGT GGTCTCAAGCTCCGACAGCGGCCCTACCTCT AGTACACCACTGCCCCGAGCCTTCCCTAGACC AAGGGTGGGGCTGTATTCTCGCGCTCTGCAG CAGGTGCAGGTCACTGATCCCGCATTCCTGA GTACCCCTCAGAGGCTGGTGACATACGACAA CCCCGTCTATGAGGGAGAAGATGTGTCCCTG CAGTTTACCCACGAGTCTATCCATAATGCTCC AGACGAAGCATTCATGGATATCATTCGCCTG CACCGACCCGCTATCACAAGCCGGAGAGGCC TGGTGCGGTTTTCCAGAATTGGACAGAGGGG CTCAATGTACACTCGCAGCGGGCAGCACATC GGAGCACGCATTCATTATTTCCAGGATATCAG CCCTGTGACTCAGGCAGCCGAGGAAATTGAG CTGCACCCACTGGTGGCTGCAGAAAATGACA CCTTCGATATCTACGCCGAGCCATTTGACCCC ATTCCTGATCCAGTGCAGCATTCCGTCACACA GTCTTATCTGACAAGTACTCCCAACACTCTGT CACAGAGCTGGGGCAATACAACTGTCCCACT GTCAATCCCCAGCGACTGGTTCGTGCAGTCT GGGCCTGATATTACTTTTCCAACCGCCTCAAT GGGAACACCCTTCAGCCCTGTCACACCAGCTC TGCCCACTGGACCTGTGTTCATCACTGGCAGC GACTTTTACCTGCACCCTACCTGGTATTTCGC CAGGCGCCGACGGAAAAGGATTCCACTGTTC TTTACCGATGTGGCCGCT MKPRARRRKRASATQLYQTCKATGT CPPDVIPKVEHTTIADQILKWGSLGV FFGGLGIGTGAGSGGRAGYIPLGSSP KPAITGGPAARPPVLVEPVAPSDPSI VSLIEESAIINAGAPEVVPPTQGGFTI TSSESTTPAILDVSVTNHTTTSVFQNP LFTEPSVIQPQPPVEASGHILISAPTIT SQHVEDIPLDTFVVSSSDSGPTSSTPL PRAFPRPRVGLYSRALQQVQVTDPA FLSTPQRLVTYDNPVYEGEDVSLQFT HESIHNAPDEAFMDIIRLHRPAITSRR GLVRFSRIGQRGSMYTRSGQHIGARI HYFQDISPVTQAAEEIELHPLVAAEN DTFDIYAEPFDPIPDPVQHSVTQSYL TSTPNTLSQSWGNTTVPLSIPSDWF VQSGPDITFPTASMGTPFSPVTPALP TGPVFITGSDFYLHPTWYFARRRRKR IPLFFTDVAA Page 8 of 48 % Nucleotide Change 26.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV16 E1 ATGGCTGATCCTGCAGGTACCAATGGGGAAG AGGGTACGGGATGTAATGGATGGTTTTATGT AGAGGCTGTAGTGGAAAAAAAAACAGGGGA TGCTATATCAGATGACGAGAACGAAAATGAC AGTGATACAGGTGAAGATTTGGTAGATTTTA TAGTAAATGATAATGATTATTTAACACAGGCA GAAACAGAGACAGCACATGCGTTGTTTACTG CACAGGAAGCAAAACAACATAGAGATGCAG TACAGGTTCTAAAACGAAAGTATTTGGGTAG TCCACTTAGTGATATTAGTGGATGTGTAGAC AATAATATTAGTCCTAGATTAAAAGCTATATG TATAGAAAAACAAAGTAGAGCTGCAAAAAG GAGATTATTTGAAAGCGAAGACAGCGGGTAT GGCAATACTGAAGTGGAAACTCAGCAGATGT TACAGGTAGAAGGGCGCCATGAGACTGAAA CACCATGTAGTCAGTATAGTGGTGGAAGTGG GGGTGGTTGCAGTCAGTACAGTAGTGGAAG TGGGGGAGAGGGTGTTAGTGAAAGACACAC TATATGCCAAACACCACTTACAAATATTTTAA ATGTACTAAAAACTAGTAATGCAAAGGCAGC AATGTTAGCAAAATTTAAAGAGTTATACGGG GTGAGTTTTTCAGAATTAGTAAGACCATTTAA AAGTAATAAATCAACGTGTTGCGATTGGTGT ATTGCTGCATTTGGACTTACACCCAGTATAGC TGACAGTATAAAAACACTATTACAACAATATT GTTTATATTTACACATTCAAAGTTTAGCATGT TCATGGGGAATGGTTGTGTTACTATTAGTAA GATATAAATGTGGAAAAAATAGAGAAACAAT TGAAAAATTGCTGTCTAAACTATTATGTGTGT CTCCAATGTGTATGATGATAGAGCCTCCAAA ATTGCGTAGTACAGCAGCAGCATTATATTGG TATAAAACAGGTATATCAAATATTAGTGAAG TGTATGGAGACACGCCAGAATGGATACAAAG ACAAACAGTATTACAACATAGTTTTAATGATT GTACATTTGAATTATCACAGATGGTACAATG GGCCTACGATAATGACATAGTAGACGATAGT GAAATTGCATATAAATATGCACAATTGGCAG ACACTAATAGTAATGCAAGTGCCTTTCTAAAA AGTAATTCACAGGCAAAAATTGTAAAGGATT GTGCAACAATGTGTAGACATTATAAACGAGC AGAAAAAAAACAAATGAGTATGAGTCAATG GATAAAATATAGATGTGATAGGGTAGATGAT GGAGGTGATTGGAAGCAAATTGTTATGTTTT TAAGGTATCAAGGTGTAGAGTTTATGTCATTT TTAACTGCATTAAAAAGATTTTTGCAAGGCAT ACCTAAAAAAAATTGCATATTACTATATGGTG CAGCTAACACAGGTAAATCATTATTTGGTATG AGTTTAATGAAATTTCTGCAAGGGTCTGTAAT ATGTTTTGTAAATTCTAAAAGCCATTTTTGGT TACAACCATTAGCAGATGCCAAAATAGGTAT GTTAGATGATGCTACAGTGCCCTGTTGGAAC TACATAGATGACAATTTAAGAAATGCATTGG ATGGAAATTTAGTTTCTATGGATGTAAAGCAT AGACCATTGGTACAACTAAAATGCCCTCCATT ATTAATTACATCTAACATTAATGCTGGTACAG ATTCTAGGTGGCCTTATTTACATAATAGATTG GTGGTGTTTACATTTCCTAATGAGTTTCCATTT GACGAAAACGGAAATCCAGTGTATGAGCTTA ATGATAAGAACTGGAAATCCTTTTTCTCAAGG ACGTGGTCCAGATTAAGTTTGCACGAGGACG AGGACAAGGAAAACGATGGAGACTCTTTGCC AACGTTTAAATGTGTGTCAGGACAAAATACT AACACATTATGA ATGGCAGACCCCGCTGGGACTAACGGAGAA GAAGGCACTGGCTGTAATGGCTGGTTTTATG TGGAGGCTGTGGTGGAGAAGAAGACAGGCG ACGCCATCTCAGACGATGAGAACGAAAATGA CAGCGATACCGGGGAGGACCTGGTGGATTTC ATTGTCAACGACAATGATTACCTGACCCAGG CAGAGACCGAAACAGCACACGCCCTGTTTAC AGCACAGGAAGCCAAGCAGCATAGGGATGC CGTGCAGGTCCTGAAGCGCAAATATCTGGGG AGCCCCCTGTCCGACATCTCTGGATGCGTGG ATAACAATATTAGCCCTCGACTGAAGGCCATC TGTATTGAGAAACAGAGCCGGGCCGCTAAGC GGAGACTGTTCGAGAGTGAAGACTCAGGCTA CGGGAACACTGAGGTGGAAACCCAGCAGAT GCTCCAGGTCGAGGGCAGGCACGAGACTGA AACCCCATGCAGCCAGTACTCCGGAGGGTCT GGAGGAGGGTGTTCACAGTATAGCTCCGGA AGCGGAGGAGAGGGCGTGTCCGAACGCCAT ACTATCTGCCAGACCCCCCTGACAAACATTCT GAATGTCCTGAAGACCAGCAACGCCAAAGCA GCCATGCTGGCTAAGTTCAAAGAGCTGTACG GGGTGTCTTTCAGTGAACTGGTCCGGCCTTTT AAGAGTAACAAGAGCACCTGCTGTGACTGGT GTATCGCTGCATTTGGCCTGACTCCAAGTATC GCTGATTCAATTAAGACCCTGCTCCAGCAGTA CTGCCTGTATCTGCACATTCAGAGCCTGGCCT GTTCCTGGGGGATGGTGGTCCTGCTGCTGGT GCGCTATAAGTGCGGAAAAAACCGAGAGAC TATCGAAAAGCTGCTGTCTAAACTGCTGTGC GTGAGTCCTATGTGTATGATGATTGAGCCCC CTAAACTGCGGAGCACAGCCGCTGCACTGTA CTGGTATAAGACTGGCATCAGCAATATTTCCG AGGTGTACGGGGACACCCCAGAATGGATTCA GAGACAGACAGTCCTCCAGCACTCCTTCAAC GATTGTACCTTTGAGCTGTCTCAGATGGTGCA GTGGGCTTATGACAATGATATCGTGGACGAT TCCGAAATTGCATACAAATATGCTCAGCTGGC AGACACCAACTCTAATGCTAGTGCATTCCTGA AGTCAAACAGCCAGGCAAAGATCGTGAAAG ATTGCGCCACAATGTGCCGGCACTACAAGCG GGCTGAGAAGAAACAGATGTCCATGTCTCAG TGGATCAAATATAGGTGCGACCGCGTGGACG ATGGGGGAGATTGGAAGCAGATTGTGATGT TCCTGAGATACCAGGGAGTCGAGTTCATGTC CTTTCTGACTGCCCTGAAGCGGTTCCTCCAGG GCATCCCCAAGAAAAACTGCATTCTGCTGTAT GGGGCCGCTAATACCGGAAAATCTCTGTTCG GCATGAGTCTGATGAAGTTTCTCCAGGGGTC TGTGATCTGTTTCGTCAATAGTAAATCACACT TTTGGCTCCAGCCACTGGCCGACGCTAAGAT CGGAATGCTGGACGATGCCACCGTGCCCTGC TGGAACTACATTGACGATAACCTGCGCAATG CTCTGGACGGCAATCTGGTGAGCATGGATGT CAAACACCGACCCCTGGTGCAGCTGAAGTGT CCACCCCTGCTGATCACATCCAACATTAATGC CGGCACTGACTCTCGGTGGCCCTACCTGCAT AACAGACTGGTGGTCTTCACATTTCCTAATGA GTTCCCATTTGACGAAAACGGCAATCCTGTGT ATGAGCTGAACGATAAGAACTGGAAATCATT CTTTAGCAGAACATGGTCCAGGCTGTCTCTGC ATGAGGACGAAGATAAAGAAAACGACGGAG ATAGTCTGCCTACTTTTAAGTGCGTGAGCGG CCAGAACACAAATACTCTG MADPAGTNGEEGTGCNGWFYVEA VVEKKTGDAISDDENENDSDTGEDL VDFIVNDNDYLTQAETETAHALFTA QEAKQHRDAVQVLKRKYLGSPLSDIS GCVDNNISPRLKAICIEKQSRAAKRRL FESEDSGYGNTEVETQQMLQVEGR HETETPCSQYSGGSGGGCSQYSSGS GGEGVSERHTICQTPLTNILNVLKTS NAKAAMLAKFKELYGVSFSELVRPFK SNKSTCCDWCIAAFGLTPSIADSIKTL LQQYCLYLHIQSLACSWGMVVLLLV RYKCGKNRETIEKLLSKLLCVSPMCM MIEPPKLRSTAAALYWYKTGISNISEV YGDTPEWIQRQTVLQHSFNDCTFEL SQMVQWAYDNDIVDDSEIAYKYAQ LADTNSNASAFLKSNSQAKIVKDCAT MCRHYKRAEKKQMSMSQWIKYRC DRVDDGGDWKQIVMFLRYQGVEF MSFLTALKRFLQGIPKKNCILLYGAA NTGKSLFGMSLMKFLQGSVICFVNS KSHFWLQPLADAKIGMLDDATVPC WNYIDDNLRNALDGNLVSMDVKHR PLVQLKCPPLLITSNINAGTDSRWPY LHNRLVVFTFPNEFPFDENGNPVYEL NDKNWKSFFSRTWSRLSLHEDEDKE NDGDSLPTFKCVSGQNTNTL Page 9 of 48 % Nucleotide Change 26.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV16 E2 ATGGAGACTCTTTGCCAACGTTTAAATGTGTG TCAGGACAAAATACTAACACATTATGAAAAT GATAGTACAGACCTACGTGACCATATAGACT ATTGGAAACACATGCGCCTAGAATGTGCTAT TTATTACAAGGCCAGAGAAATGGGATTTAAA CATATTAACCACCAGGTGGTGCCAACACTGG CTGTATCAAAGAATAAAGCATTACAAGCAAT TGAACTGCAACTAACGTTAGAAACAATATAT AACTCACAATATAGTAATGAAAAGTGGACAT TACAAGACGTTAGCCTTGAAGTGTATTTAACT GCACCAACAGGATGTATAAAAAAACATGGAT ATACAGTGGAAGTGCAGTTTGATGGAGACAT ATGCAATACAATGCATTATACAAACTGGACA CATATATATATTTGTGAAGAAGCATCAGTAAC TGTGGTAGAGGGTCAAGTTGACTATTATGGT TTATATTATGTTCATGAAGGAATACGAACATA TTTTGTGCAGTTTAAAGATGATGCAGAAAAA TATAGTAAAAATAAAGTATGGGAAGTTCATG CGGGTGGTCAGGTAATATTATGTCCTACATCT GTGTTTAGCAGCAACGAAGTATCCTCTCCTGA AATTATTAGGCAGCACTTGGCCAACCACCCC GCCGCGACCCATACCAAAGCCGTCGCCTTGG GCACCGAAGAAACACAGACGACTATCCAGCG ACCAAGATCAGAGCCAGACACCGGAAACCCC TGCCACACCACTAAGTTGTTGCACAGAGACTC AGTGGACAGTGCTCCAATCCTCACTGCATTTA ACAGCTCACACAAAGGACGGATTAACTGTAA TAGTAACACTACACCCATAGTACATTTAAAAG GTGATGCTAATACTTTAAAATGTTTAAGATAT AGATTTAAAAAGCATTGTACATTGTATACTGC AGTGTCGTCTACATGGCATTGGACAGGACAT AATGTAAAACATAAAAGTGCAATTGTTACACT TACATATGATAGTGAATGGCAACGTGACCAA TTTTTGTCTCAAGTTAAAATACCAAAAACTAT TACAGTGTCTACTGGATTTATGTCTATATGA METLCQRLNVCQDKILTHYENDSTD LRDHIDYWKHMRLECAIYYKAREMG FKHINHQVVPTLAVSKNKALQAIELQ LTLETIYNSQYSNEKWTLQDVSLEVY LTAPTGCIKKHGYTVEVQFDGDICNT MHYTNWTHIYICEEASVTVVEGQVD YYGLYYVHEGIRTYFVQFKDDAEKYS KNKVWEVHAGGQVILCPTSVFSSNE VSSPEIIRQHLANHPAATHTKAVALG TEETQTTIQRPRSEPDTGNPCHTTKL LHRDSVDSAPILTAFNSSHKGRINCN SNTTPIVHLKGDANTLKCLRYRFKKH CTLYTAVSSTWHWTGHNVKHKSAIV TLTYDSEWQRDQFLSQVKIPKTITVS TGFMSI HPV16 E4 TATTATGTCCTACATCTGTGTTTAGCAGCAAC GAAGTATCCTCTCCTGAAATTATTAGGCAGCA CTTGGCCAACCACCCCGCCGCGACCCATACCA AAGCCGTCGCCTTGGGCACCGAAGAAACACA GACGACTATCCAGCGACCAAGATCAGAGCCA GACACCGGAAACCCCTGCCACACCACTAAGT TGTTGCACAGAGACTCAGTGGACAGTGCTCC AATCCTCACTGCATTTAACAGCTCACACAAAG GACGGATTAACTGTAATAGTAACACTACACC CATAG ATGACAAATCTTGATACTGCATCCACAACATT ACTGGCGTGCTTTTTGCTTTGCTTTTGTGTGCT TTTGTGTGTCTGCCTATTAATACGTCCGCTGC TTTTGTCTGTGTCTACATACACATCATTAATAA TATTGGTATTACTATTGTGGATAACAGCAGCC TCTGCGTTTAGGTGTTTTATTGTATATATTATA TTTGTTTATATACCATTATTTTTAATACATACA CATGCACGCTTTTTAATTACATAA ATGCACCAAAAGAGAACTGCAATGTTTCAGG ACCCACAGGAGCGACCCAGAAAGTTACCACA GTTATGCACAGAGCTGCAAACAACTATACAT GATATAATATTAGAATGTGTGTACTGCAAGC AACAGTTACTGCGACGTGAGGTATATGACTT TGCTTTTCGGGATTTATGCATAGTATATAGAG ATGGGAATCCATATGCTGTATGTGATAAATG TTTAAAGTTTTATTCTAAAATTAGTGAGTATA GACATTATTGTTATAGTTTGTATGGAACAACA TTAGAACAGCAATACAACAAACCGTTGTGTG ATTTGTTAATTAGGTGTATTAACTGTCAAAAG CCACTGTGTCCTGAAGAAAAGCAAAGACATC TGGACAAAAAGCAAAGATTCCATAATATAAG GGGTCGGTGGACCGGTCGATGTATGTCTTGT TGCAGATCATCAAGAACACGTAGAGAAACCC AGCTGTAA ATGGAGACTCTGTGCCAGCGGCTGAACGTGT GCCAGGATAAGATTCTGACTCACTACGAAAA TGACTCAACCGACCTGCGGGACCACATCGAC TACTGGAAGCACATGCGACTGGAGTGCGCCA TCTACTATAAGGCTCGGGAAATGGGCTTCAA ACACATCAATCATCAGGTGGTCCCCACCCTGG CCGTGAGCAAGAACAAGGCCCTCCAGGCAAT CGAGCTGCAACTGACCCTGGAAACAATCTAC AATAGTCAGTATTCAAACGAGAAGTGGACAC TCCAGGACGTGAGCCTGGAAGTCTACCTGAC TGCACCTACCGGATGTATTAAGAAACACGGC TATACCGTGGAGGTCCAGTTTGACGGCGATA TCTGCAATACAATGCATTACACAAACTGGACT CACATCTATATTTGTGAGGAAGCTAGCGTGA CTGTGGTCGAGGGGCAGGTCGATTACTATGG ACTGTACTATGTGCATGAAGGGATTCGCACC TACTTCGTGCAGTTTAAGGACGATGCTGAGA AATATTCTAAGAACAAGGTCTGGGAAGTCCA CGCAGGAGGACAGGTCATCCTGTGCCCTACC AGTGTGTTCAGCTCCAATGAGGTCTCTAGTCC AGAAATCATTCGACAGCACCTGGCCAACCAT CCCGCCGCTACCCACACAAAGGCAGTGGCCC TGGGAACCGAGGAAACACAGACCACAATTCA GCGGCCCAGATCCGAGCCTGACACAGGCAAT CCTTGCCATACTACCAAGCTGCTGCACAGAG ACAGCGTGGATTCCGCACCAATCCTGACTGC CTTCAACTCAAGCCATAAAGGCAGGATCAAC TGTAATTCTAACACAACTCCAATTGTCCACCT GAAGGGGGATGCCAATACCCTGAAATGCCTG CGGTACAGATTCAAGAAACACTGTACTCTGT ATACCGCCGTGTCCTCTACATGGCACTGGACT GGGCATAACGTGAAGCACAAATCAGCTATCG TCACTCTGACCTACGACAGCGAGTGGCAGAG GGATCAGTTCCTGTCCCAGGTGAAGATCCCC AAAACAATTACTGTCTCTACAGGCTTCATGAG TATC ATGTATGTGCTGCATCTGTGCCTGGCTGCTAC CAAGTACCCCCTGCTGAAACTGCTGGGATCA ACCTGGCCTACCACCCCCCCTCGGCCCATCCC TAAGCCATCTCCCTGGGCCCCTAAGAAACACC GGCGGCTGAGCAGCGACCAGGATCAGTCAC AGACTCCTGAGACCCCAGCTACACCCCTGAG CTGCTGTACCGAAACACAGTGGACAGTGCTC CAGAGCAGCCTGCACCTGACTGCCCATACCA AAGACGGCCTGACAGTGATTGTCACTCTGCA TCCC ATGACCAACCTGGATACTGCTTCTACTACCCT GCTGGCTTGTTTCCTGCTGTGTTTCTGTGTCCT GCTGTGCGTGTGCCTGCTGATTAGGCCCCTG CTGCTGAGCGTGTCCACCTACACATCTCTGAT CATTCTGGTCCTGCTGCTGTGGATCACAGCCG CTAGCGCATTCCGGTGCTTCATCGTGTACATC ATCTTCGTCTACATCCCTCTGTTTCTGATTCAC ACTCATGCCAGATTCCTGATCACC ATGCACCAGAAGAGAACCGCCATGTTTCAGG ACCCTCAGGAACGACCTCGCAAACTGCCCCA GCTGTGTACCGAACTGCAGACAACTATCCAC GACATCATTCTGGAGTGCGTGTACTGTAAGC AGCAGCTGCTGCGGAGAGAAGTCTATGACTT CGCCTTTCGCGATCTGTGCATCGTGTACCGAG ACGGAAACCCCTACGCCGTCTGCGATAAGTG TCTGAAGTTCTACTCTAAGATTAGTGAGTATC GGCATTACTGTTATAGCCTGTACGGCACCACA CTGGAACAGCAGTATAACAAACCCCTGTGCG ACCTGCTGATCAGATGCATTAATTGTCAGAA GCCCCTGTGTCCTGAGGAAAAACAGAGGCAC CTGGATAAGAAACAGCGCTTTCATAATATTCG AGGCCGGTGGACAGGGAGGTGCATGTCTTG CTGTAGAAGCTCCAGGACTAGGCGCGAGACC CAGCTG HPV16 E5 HPV16 E6 Page 10 of 48 % Nucleotide Change 25.9% YYVLHLCLAATKYPLLKLLGSTWPTTP PRPIPKPSPWAPKKHRRLSSDQDQS QTPETPATPLSCCTETQWTVLQSSLH LTAHTKDGLTVIVTLHP 29.4% MTNLDTASTTLLACFLLCFCVLLCVCL LIRPLLLSVSTYTSLIILVLLLWITAASAF RCFIVYIIFVYIPLFLIHTHARFLIT 35.0% MHQKRTAMFQDPQERPRKLPQLCT ELQTTIHDIILECVYCKQQLLRREVYD FAFRDLCIVYRDGNPYAVCDKCLKFY SKISEYRHYCYSLYGTTLEQQYNKPLC DLLIRCINCQKPLCPEEKQRHLDKKQ RFHNIRGRWTGRCMSCCRSSRTRRE TQL 25.4% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV16 E7 ATGCATGGAGATACACCTACATTGCATGAAT ATATGTTAGATTTGCAACCAGAGACAACTGA TCTCTACTGTTATGAGCAATTAAATGACAGCT CAGAGGAGGAGGATGAAATAGATGGTCCAG CTGGACAAGCAGAACCGGACAGAGCCCATTA CAATATTGTAACCTTTTGTTGCAAGTGTGACT CTACGCTTCGGTTGTGCGTACAAAGCACACA CGTAGACATTCGTACTTTGGAAGACCTGTTAA TGGGCACACTAGGAATTGTGTGCCCCATCTG TTCTCAGAAACCATAA ATGTCTCTTTGGCTGCCTAGTGAGGCCACTGT CTACTTGCCTCCTGTCCCAGTATCTAAGGTTG TAAGCACGGATGAATATGTTGCACGCACAAA CATATATTATCATGCAGGAACATCCAGACTAC TTGCAGTTGGACATCCCTATTTTCCTATTAAA AAACCTAACAATAACAAAATATTAGTTCCTAA AGTATCAGGATTACAATACAGGGTATTTAGA ATACATTTACCTGACCCCAATAAGTTTGGTTT TCCTGACACCTCATTTTATAATCCAGATACAC AGCGGCTGGTTTGGGCCTGTGTAGGTGTTGA GGTAGGTCGTGGTCAGCCATTAGGTGTGGGC ATTAGTGGCCATCCTTTATTAAATAAATTGGA TGACACAGAAAATGCTAGTGCTTATGCAGCA AATGCAGGTGTGGATAATAGAGAATGTATAT CTATGGATTACAAACAAACACAATTGTGTTTA ATTGGTTGCAAACCACCTATAGGGGAACACT GGGGCAAAGGATCCCCATGTACCAATGTTGC AGTAAATCCAGGTGATTGTCCACCATTAGAG TTAATAAACACAGTTATTCAGGATGGTGATAT GGTTGATACTGGCTTTGGTGCTATGGACTTTA CTACATTACAGGCTAACAAAAGTGAAGTTCC ACTGGATATTTGTACATCTATTTGCAAATATC CAGATTATATTAAAATGGTGTCAGAACCATAT GGCGACAGCTTATTTTTTTATTTACGAAGGGA ACAAATGTTTGTTAGACATTTATTTAATAGGG CTGGTACTGTTGGTGAAAATGTACCAGACGA TTTATACATTAAAGGCTCTGGGTCTACTGCAA ATTTAGCCAGTTCAAATTATTTTCCTACACCTA GTGGTTCTATGGTTACCTCTGATGCCCAAATA TTCAATAAACCTTATTGGTTACAACGAGCACA GGGCCACAATAATGGCATTTGTTGGGGTAAC CAACTATTTGTTACTGTTGTTGATACTACACG CAGTACAAATATGTCATTATGTGCTGCCATAT CTACTTCAGAAACTACATATAAAAATACTAAC TTTAAGGAGTACCTACGACATGGGGAGGAAT ATGATTTACAGTTTATTTTTCAACTGTGCAAA ATAACCTTAACTGCAGACGTTATGACATACAT ACATTCTATGAATTCCACTATTTTGGAGGACT GGAATTTTGGTCTACAACCTCCCCCAGGAGG CACACTAGAAGATACTTATAGGTTTGTAACAT CCCAGGCAATTGCTTGTCAAAAACATACACCT CCAGCACCTAAAGAAGATCCCCTTAAAAAAT ACACTTTTTGGGAAGTAAATTTAAAGGAAAA GTTTTCTGCAGACCTAGATCAGTTTCCTTTAG GACGCAAATTTTTACTACAAGCAGGATTGAA GGCCAAACCAAAATTTACATTAGGAAAACGA AAAGCTACACCCACCACCTCATCTACCTCTAC AACTGCTAAACGCAAAAAACGTAAGCTGTAA ATGCACGGCGACACTCCTACTCTGCACGAAT ACATGCTGGACCTGCAGCCCGAAACTACTGA CCTGTACTGCTACGAACAGCTGAATGACAGC TCCGAGGAAGAGGACGAAATCGATGGACCT GCCGGCCAGGCTGAGCCTGACAGGGCCCACT ACAACATTGTGACTTTCTGCTGTAAGTGCGAT TCTACCCTGCGGCTGTGTGTGCAGAGTACCC ATGTGGACATCAGAACCCTGGAGGACCTGCT GATGGGAACACTGGGCATCGTCTGCCCAATT TGTTCCCAGAAACCC ATGCAGGTCACTTTTATCTATATCCTGGTCAT TACTTGCTACGAGAACGATGTCAACGTCTATC ATATTTTCTTTCAGATGTCCCTGTGGCTGCCA TCCGAGGCAACCGTCTACCTGCCCCCTGTGCC CGTCTCTAAAGTGGTCAGTACAGATGAATAT GTGGCCCGCACTAACATCTACTATCACGCCG GGACATCTCGACTGCTGGCTGTCGGACATCC CTACTTCCCTATCAAGAAACCCAACAACAACA AAATTCTGGTCCCTAAGGTGAGTGGCCTCCA GTATAGGGTGTTCCGCATTCACCTGCCAGATC CCAATAAGTTCGGGTTTCCTGACACCAGCTTT TACAACCCAGATACACAGCGACTGGTCTGGG CATGCGTGGGAGTCGAAGTGGGAAGAGGAC AGCCTCTGGGAGTGGGAATCAGCGGACATCC ACTGCTGAACAAGCTGGACGATACCGAGAAC GCTTCCGCATACGCCGCTAATGCTGGCGTGG ACAACCGGGAATGTATTTCTATGGATTATAA GCAGACACAGCTGTGCCTGATCGGATGTAAA CCACCCATTGGAGAGCACTGGGGCAAGGGG TCCCCATGCACTAATGTCGCCGTGAACCCCG GCGACTGTCCTCCACTGGAACTGATCAATACC GTCATTCAGGACGGAGATATGGTGCATACAG GATTCGGCGCAATGGATTTTACCACACTCCAG GCCAACAAGAGTGAGGTGCCCCTGGACATCT GCACCTCAATTTGTAAGTACCCCGATTACATC AAGATGGTGTCCGAGCCTTACGGGGACTCTC TGTTCTTTTATCTGCGGAGAGAACAGATGTTC GTGAGACACCTGTTTAATAGGGCAGGCACTG TCGGAGAAAACGTGCCAGACGATCTGTACAT CAAGGGGTCAGGAAGCACCGCAAATCTGGC CAGCTCCAACTATTTCCCTACTCCATCCGGCT CTATGGTGACCTCTGACGCCCAGATTTTCAAC AAGCCTTACTGGCTCCAGCGGGCCCAGGGAC ATAATAACGGCATTTGCTGGGGGAATCAGCT GTTCGTGACAGTGGTCGATACTACCCGCTCA ACTAACATGAGCCTGTGTGCAGCCATCAGTA CCTCAGAGACAACTTACAAGAACACAAACTT CAAGGAATACCTGAGACACGGAGAGGAATA TGACCTCCAGTTCATCTTTCAGCTGTGCAAGA TTACACTGACTGCCGATGTGATGACTTACATC CATAGCATGAACAGCACCATTCTGGAGGACT GGAACTTCGGACTCCAGCCACCTCCAGGCGG GACCCTGGAAGATACATATAGGTTTGTGACA CAGGCCATCGCTTGTCAGAAACACACTCCCCC TGCTCCAAAGGAGGACGATCCCCTGAAGAAA TACACCTTCTGGGAGGTGAACCTGAAGGAAA AGTTCAGCGCCGACCTGGACCAGTTCCCACT GGGCAGGAAGTTTCTGCTCCAGGCTGGGCTG AAGGCAAAACCTAAGTTCACACTGGGCAAAC GCAAGGCTACTCCAACCACATCTAGTACCAG CACTACCGCAAAACGAAAGAAACGGAAGCT G MHGDTPTLHEYMLDLQPETTDLYCY EQLNDSSEEEDEIDGPAGQAEPDRA HYNIVTFCCKCDSTLRLCVQSTHVDI RTLEDLLMGTLGIVCPICSQKP HPV16 L1 Page 11 of 48 MSLWLPSEATVYLPPVPVSKVVSTD EYVARTNIYYHAGTSRLLAVGHPYFPI KKPNNNKILVPKVSGLQYRVFRIHLP DPNKFGFPDTSFYNPDTQRLVWAC VGVEVGRGQPLGVGISGHPLLNKLD DTENASAYAANAGVDNRECISMDY KQTQLCLIGCKPPIGEHWGKGSPCT NVAVNPGDCPPLELINTVIQDGDMV DTGFGAMDFTTLQANKSEVPLDICT SICKYPDYIKMVSEPYGDSLFFYLRRE QMFVRHLFNRAGTVGENVPDDLYIK GSGSTANLASSNYFPTPSGSMVTSD AQIFNKPYWLQRAQGHNNGICWG NQLFVTVVDTTRSTNMSLCAAISTSE TTYKNTNFKEYLRHGEEYDLQFIFQL CKITLTADVMTYIHSMNSTILEDWNF GLQPPPGGTLEDTYRFVTSQAIACQK HTPPAPKEDPLKKYTFWEVNLKEKFS ADLDQFPLGRKFLLQAGLKAKPKFTL GKRKATPTTSSTSTTAKRKKRKL % Nucleotide Change 25.6% 30.0% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV16 L2 ATGCGACACAAACGTTCTGCAAAACGCACAA AACGTGCATCGGCTACCCAACTTTATAAAACA TGCAAACAGGCAGGTACATGTCCACCTGACA TTATACCTAAGGTTGAAGGCAAAACTATTGCT GATCAAATATTACAATATGGAAGTATGGGTG TATTTTTTGGTGGGTTAGGAATTGGAACAGG GTCGGGTACAGGCGGACGCACTGGGTATATT CCATTGGGAACAAGGCCTCCCACAGCTACAG ATACACTTGCTCCTGTAAGACCCCCTTTAACA GTAGATCCTGTGGGCCCTTCTGATCCTTCTAT AGTTTCTTTAGTGGAAGAAACTAGTTTTATTG ATGCTGGTGCACCAACATCTGTACCTTCCATT CCCCCAGATGTATCAGGATTTAGTATTACTAC TTCAACTGATACCACACCTGCTATATTAGATA TTAATAATACTGTTACTACTGTTACTACACATA ATAATCCCACTTTCACTGACCCATCTGTATTG CAGCCTCCAACACCTGCAGAAACTGGAGGGC ATTTTACACTTTCATCATCCACTATTAGTACAC ATAATTATGAAGAAATTCCTATGGATACATTT ATTGTTAGCACAAACCCTAACACAGTAACTAG TAGCACACCCATACCAGGGTCTCGCCCAGTG GCACGCCTAGGATTATATAGTCGCACAACAC AACAGGTTAAAGTTGTAGACCCTGCTTTTGTA ACCACTCCCACTAAACTTATTACATATGATAA TCCTGCATATGAAGGTATAGATGTGGATAAT ACATTATATTTTTCTAGTAATGATAATAGTATT AATATAGCTCCAGATCCTGACTTTTTGGATAT AGTTGCTTTACATAGGCCAGCATTAACCTCTA GGCGTACTGGCATTAGGTACAGTAGAATTGG TAATAAACAAACACTACGTACTCGTAGTGGA AAATCTATAGGTGCTAAGGTACATTATTATTA TGATTTAAGTACTATTGATCCTGCAGAAGAAA TAGAATTACAAACTATAACACCTTCTACATAT ACTACCACTTCACATGCAGCCTCACCTACTTCT ATTAATAATGGATTATATGATATTTATGCAGA TGACTTTATTACAGATACTTCTACAACCCCGG TACCATCTGTACCCTCTACATCTTTATCAGGTT ATATTCCTGCAAATACAACAATTCCTTTTGGT GGTGCATACAATATTCCTTTAGTATCAGGTCC TGATATACCCATTAATATAACTGACCAAGCTC CTTCATTAATTCCTATAGTTCCAGGGTCTCCA CAATATACAATTATTGCTGATGCAGGTGACTT TTATTTACATCCTAGTTATTACATGTTACGAA AACGACGTAAACGTTTACCATATTTTTTTTCA GATGTCTCTTTGGCTGCCTAG ATGAGACATAAGCGGAGTGCTAAGAGGACT AAAAGAGCCAGCGCCACCCAGCTGTATAAGA CTTGTAAACAGGCCGGAACTTGCCCTCCTGAC ATCATTCCAAAGGTGGAGGGGAAAACCATTG CCGAACAGATCCTCCAGTATGGCAGTATGGG GGTCTTCTTTGGCGGGCTGGGAATTGGCACA GGGTCTGGAACTGGAGGCAGGACCGGGTAC ATCCCACTGGGAACACGCCCCCCTACAGCAA CTGATACCCTGGCACCCGTGAGACCACCCCT GACCGTGGACCCAGTCGGACCAAGCGATCCT TCCATTGTGTCTCTGGTCGAGGAAACCTCCTT CATCGACGCTGGCGCACCCACAAGTGTGCCT TCAATTCCTCCAGATGTCAGCGGGTTTTCCAT CACCACATCTACAGACACTACCCCAGCCATTC TGGATATCAACAATACTGTGACAACTGTCACC ACACACAACAATCCAACATTCACTGACCCCTC CGTGCTCCAGCCACCTACACCCGCTGAGACT GGAGGACACTTTACACTGAGCAGCAGCACCA TCAGCACACATAACTATGAGGAAATTCCAAT GGATACATTCATCGTGAGCACTAACCCCAATA CCGTCACAAGTTCAACCCCAATCCCCGGCAGC CGGCCCGTGGCACGACTGGGCCTGTACTCTC GAACTACCCAGCAGGTGAAGGTGGTGGACC CCGCTTTTGTCACAACTCCAACCAAACTGATT ACCTACGACAACCCCGCATACGAAGGCATCG ACGTGGATAATACCCTGTATTTCAGCTCCAAC GACAATAGCATCAACATTGCCCCTGACCCAG ATTTTCTGGATATCGTGGCTCTGCATCGCCCC GCACTGACTTCTCGGAGAACCGGCATTAGAT ACAGTAGGATCGGGAATAAGCAGACTCTGCG AACCCGGTCTGGAAAGAGTATTGGCGCAAAA GTGCACTACTATTACGACCTGAGCACCATCGA TCCTGCCGAGGAAATTGAGCTGCAAACTATC ACCCCAAGTACTTATACCACAACTTCACATGC CGCTTCACCCACCAGCATCAACAATGGCCTGT ATGACATCTACGCAGACGATTTCATCACAGAT ACTAGCACCACACCCGTGCCTTCCGTCCCTTC TACCAGTCTGTCAGGATATATTCCCGCCAACA CTACCATCCCTTTTGGCGGGGCTTACAATATC CCTCTGGTGAGCGGCCCAGACATCCCCATTA ATATCACAGATCAGGCTCCATCACTGATTCCT ATCGTCCCAGGGAGCCCCCAGTATACCATCAT TGCCGACGCTGGAGATTTCTACCTGCACCCCT CCTATTACATGCTGCGGAAGAGGCGCAAAAG ACTGCCTTACTTCTTTTCCGACGTGTCTCTGGC AGCC MRHKRSAKRTKRASATQLYKTCKQA GTCPPDIIPKVEGKTIADQILQYGSM GVFFGGLGIGTGSGTGGRTGYIPLGT RPPTATDTLAPVRPPLTVDPVGPSDP SIVSLVEETSFIDAGAPTSVPSIPPDVS GFSITTSTDTTPAILDINNTVTTVTTH NNPTFTDPSVLQPPTPAETGGHFTLS SSTISTHNYEEIPMDTFIVSTNPNTVT SSTPIPGSRPVARLGLYSRTTQQVKV VDPAFVTTPTKLITYDNPAYEGIDVD NTLYFSSNDNSINIAPDPDFLDIVALH RPALTSRRTGIRYSRIGNKQTLRTRSG KSIGAKVHYYYDLSTIDPAEEIELQTIT PSTYTTTSHAASPTSINNGLYDIYADD FITDTSTTPVPSVPSTSLSGYIPANTTI PFGGAYNIPLVSGPDIPINITDQAPSLI PIVPGSPQYTIIADAGDFYLHPSYYML RKRRKRLPYFFSDVSLAA Page 12 of 48 % Nucleotide Change 28.3% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV18 E1 ATGGCTGATCCAGAAGGTACAGACGGGGAG GGCACGGGTTGTAACGGCTGGTTTTATGTAC AAGCTATTGTAGACAAAAAAACAGGAGATGT AATATCAGATGACGAGGACGAAAATGCAACA GACACAGGGTCGGATATGGTAGATTTTATTG ATACACAAGGAACATTTTGTGAACAGGCAGA GCTAGAGACAGCACAGGCATTGTTCCATGCG CAGGAGGTCCACAATGATGCACAAGTGTTGC ATGTTTTAAAACGAAAGTTTGCAGGAGGCAG CACAGAAAACAGTCCATTAGGGGAGCGGCT GGAGGTGGATACAGAGTTAAGTCCACGGTTA CAAGAAATATCTTTAAATAGTGGGCAGAAAA AGGCAAAAAGGCGGCTGTTTACAATATCAGA TAGTGGCTATGGCTGTTCTGAAGTGGAAGCA ACACAGATTCAGGTAACTACAAATGGCGAAC ATGGCGGCAATGTATGTAGTGGCGGCAGTAC GGAGGCTATAGACAACGGGGGCACAGAGGG CAACAACAGCAGTGTAGACGGTACAAGTGAC AATAGCAATATAGAAAATGTAAATCCACAAT GTACCATAGCACAATTAAAAGACTTGTTAAA AGTAAACAATAAACAAGGAGCTATGTTAGCA GTATTTAAAGACACATATGGGCTATCATTTAC AGATTTAGTTAGAAATTTTAAAAGTGATAAA ACCACGTGTACAGATTGGGTTACAGCTATATT TGGAGTAAACCCAACAATAGCAGAAGGATTT AAAACACTAATACAGCCATTTATATTATATGC CCATATTCAATGTCTAGACTGTAAATGGGGA GTATTAATATTAGCCCTGTTGCGTTACAAATG TGGTAAGAGTAGACTAACAGTTGCTAAAGGT TTAAGTACGTTGTTACACGTACCTGAAACTTG TATGTTAATTCAACCACCAAAATTGCGAAGTA GTGTTGCAGCACTATATTGGTATAGAACAGG AATATCAAATATTAGTGAAGTAATGGGAGAC ACACCTGAGTGGATACAAAGACTTACTATTAT ACAACATGGAATAGATGATAGCAATTTTGAT TTGTCAGAAATGGTACAATGGGCATTTGATA ATGAGCTGACAGATGAAAGCGATATGGCATT TGAATATGCCTTATTAGCAGACAGCAACAGC AATGCAGCTGCCTTTTTAAAAAGCAATTGCCA AGCTAAATATTTAAAAGATTGTGCCACAATGT GCAAACATTATAGGCGAGCCCAAAAACGACA AATGAATATGTCACAGTGGATACGATTTAGA TGTTCAAAAATAGATGAAGGGGGAGATTGG AGACCAATAGTGCAATTCCTGCGATACCAAC AAATAGAGTTTATAACATTTTTAGGAGCCTTA AAATCATTTTTAAAAGGAACCCCCAAAAAAA ATTGTTTAGTATTTTGTGGACCAGCAAATACA GGAAAATCATATTTTGGAATGAGTTTTATACA CTTTATACAAGGAGCAGTAATATCATTTGTGA ATTCCACTAGTCATTTTTGGTTGGAACCGTTA ACAGATACTAAGGTGGCCATGTTAGATGATG CAACGACCACGTGTTGGACATACTTTGATACC TATATGAGAAATGCGTTAGATGGCAATCCAA TAAGTATTGATAGAAAGCACAAACCATTAAT ACAACTAAAATGTCCTCCAATACTACTAACCA CAAATATACATCCAGCAAAGGATAATAGATG GCCATATTTAGAAAGTAGAATAACAGTATTT GAATTTCCAAATGCATTTCCATTTGATAAAAA TGGCAATCCAGTATATGAAATAAATGACAAA AATTGGAAATGTTTTTTTGAAAGGACATGGT CCAGATTAGATTTGCACGAGGAAGAGGAAG ATGCAGACACCGAAGGAAACCCTTTCGGAAC GTTTAAGTGCGTTGCAGGACAAAATCATAGA CCACTATGA ATGGCAGACCCCGAAGGGACTGACGGCGAA GGGACTGGATGTAACGGATGGTTTTATGTGC AGGCTATTGTGGATAAGAAGACTGGCGACGT GATCAGCGACGATGAGGATGAAAACGCTACC GACACAGGCTCCGACATGGTCGATTTCATTG ACACACAGGGGACTTTTTGCGAGCAGGCAGA GCTGGAAACCGCACAGGCCCTGTTCCACGCT CAGGAAGTGCATAACGATGCACAGGTGCTGC ACGTCCTGAAGCGGAAATTTGCCGGCGGGA GTACAGAGAACAGCCCACTGGGGGAGAGAC TGGAAGTGGACACTGAGCTGTCTCCCAGGCT CCAGGAAATCAGCCTGAACTCCGGACAGAAG AAAGCCAAGCGGAGACTGTTCACCATCTCAG ATAGCGGGTACGGATGCAGCGAGGTGGAAG CTACACAGATTCAGGTCACCACAAACGGCGA GCACGGAGGCAATGTGTGTTCTGGGGGAAG TACAGAGGCTATTGACAATGGCGGGACTGAA GGAAACAATAGCTCCGTGGATGGCACTTCAG ACAACAGCAATATCGAAAACGTCAATCCTCA GTGCACCATTGCCCAGCTGAAGGATCTGCTG AAAGTGAACAATAAGCAGGGAGCTATGCTG GCAGTCTTCAAGGATACCTACGGCCTGAGTT TCACTGACCTGGTGAGAAACTTTAAGTCAGA TAAAACTACCTGTACCGACTGGGTGACAGCA ATCTTTGGGGTCAATCCCACCATTGCCGAGG GATTCAAAACACTGATCCAGCCTTTTATTCTG TACGCCCACATCCAGTGCCTGGACTGTAAGT GGGGCGTGCTGATTCTGGCCCTGCTGCGGTA TAAGTGCGGAAAATCCAGACTGACTGTGGCT AAAGGCCTGTCTACCCTGCTGCATGTCCCCGA GACATGTATGCTGATCCAGCCCCCTAAGCTGC GCTCTAGTGTGGCCGCTCTGTACTGGTATCG AACCGGGATCTCCAACATTTCTGAGGTCATG GGAGACACCCCTGAATGGATTCAGAGGCTGA CAATCATTCAGCACGGCATTGACGATAGCAA CTTCGATCTGTCCGAGATGGTGCAGTGGGCT TTTGACAATGAGCTGACCGATGAATCTGACA TGGCATTCGAATACGCCCTGCTGGCTGATTCC AACTCTAATGCAGCCGCTTTTCTGAAAAGTAA CTGCCAGGCCAAGTACCTGAAAGACTGCGCT ACAATGTGTAAACATTATAGGCGCGCCCAGA AGCGCCAGATGAATATGTCACAGTGGATCAG ATTCCGGTGCAGCAAGATTGATGAGGGAGG CGACTGGAGGCCAATCGTGCAGTTTCTGCGC TACCAGCAGATCGAGTTCATCACTTTTCTGGG CGCCCTGAAGAGCTTCCTGAAGGGGACCCCT AAGAAAAACTGCCTGGTGTTCTGTGGACCAG CTAATACAGGCAAATCTTATTTTGGGATGAGT TTCATCCACTTTATTCAGGGCGCAGTGATCAG CTTCGTCAACAGTACTTCACATTTTTGGCTGG AGCCCCTGACTGATACCAAGGTGGCAATGCT GGACGATGCCACAACTACCTGCTGGACTTAC TTCGACACCTATATGCGGAACGCCCTGGATG GGAATCCAATCAGCATTGACAGAAAGCACAA ACCCCTGATCCAGCTGAAATGTCCACCCATCC TGCTGACAACTAACATTCATCCCGCAAAGGA CAATCGATGGCCTTACCTGGAGTCCCGGATC ACCGTGTTCGAATTTCCTAATGCCTTCCCATTT GATAAGAACGGCAATCCCGTCTATGAGATTA ACGACAAGAACTGGAAGTGTTTCTTTGAAAG AACATGGTCCAGGCTGGATCTGCACGAGGAA GAGGAAGACGCAGATACTGAGGGCAACCCT TTCGGGACCTTTAAGCTGCGCGCCGGCCAGA ATCATCGACCACTG MADPEGTDGEGTGCNGWFYVQAI VDKKTGDVISDDEDENATDTGSDM VDFIDTQGTFCEQAELETAQALFHA QEVHNDAQVLHVLKRKFAGGSTENS PLGERLEVDTELSPRLQEISLNSGQKK AKRRLFTISDSGYGCSEVEATQIQVTT NGEHGGNVCSGGSTEAIDNGGTEG NNSSVDGTSDNSNIENVNPQCTIAQ LKDLLKVNNKQGAMLAVFKDTYGLS FTDLVRNFKSDKTTCTDWVTAIFGV NPTIAEGFKTLIQPFILYAHIQCLDCK WGVLILALLRYKCGKSRLTVAKGLSTL LHVPETCMLIQPPKLRSSVAALYWYR TGISNISEVMGDTPEWIQRLTIIQHGI DDSNFDLSEMVQWAFDNELTDESD MAFEYALLADSNSNAAAFLKSNCQA KYLKDCATMCKHYRRAQKRQMNM SQWIRFRCSKIDEGGDWRPIVQFLR YQQIEFITFLGALKSFLKGTPKKNCLV FCGPANTGKSYFGMSFIHFIQGAVIS FVNSTSHFWLEPLTDTKVAMLDDAT TTCWTYFDTYMRNALDGNPISIDRK HKPLIQLKCPPILLTTNIHPAKDNRW PYLESRITVFEFPNAFPFDKNGNPVY EINDKNWKCFFERTWSRLDLHEEEE DADTEGNPFGTFKCVAGQNHRPL Page 13 of 48 % Nucleotide Change 25.5% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV18 E2 ATGCAGACACCGAAGGAAACCCTTTCGGAAC GTTTAAGTGCGTTGCAGGACAAAATCATAGA CCACTATGAAAATGACAGTAAAGACATAGAC AGCCAAATACAGTATTGGCAACTAATACGTT GGGAAAATGCAATATTCTTTGCAGCAAGGGA ACATGGCATACAGACATTAAACCACCAGGTG GTGCCAGCCTATAACATTTCAAAAAGTAAAG CACATAAAGCTATTGAACTGCAAATGGCCCT ACAAGGCCTTGCACAAAGTGCATACAAAACC GAGGATTGGACACTGCAAGACACATGCGAG GAACTATGGAATACAGAACCTACTCACTGCTT TAAAAAAGGTGGCCAAACAGTACAAGTATAT TTTGATGGCAACAAAGACAATTGTATGACCT ATGTAGCATGGGACAGTGTGTATTATATGAC TGATGCAGGAACATGGGACAAAACGGCTACC TGTGTAAGTCACAGGGGATTGTATTATGTAA AGGAAGGGTACAACACGTTTTATATAGAATT TAAAAGTGAATGTGAAAAATATGGGAACACA GGTACGTGGGAAGTACATTTTGGGAATAATG TAATTGATTGTAATGACTCTATGTGCAGTACC AGTGACGACACGGTATCCGCTACTCAGCTTG TTAAACAGCTACAGCACACCCCCTCACCGTAT TCCAGCACCGTGTCCGTGGGCACCGCAAAGA CCTACGGCCAGACGTCGGCTGCTACACGACC TGGACACTGTGGACTCGCGGAGAAGCAGCAT TGTGGACCTGTCAACCCACTTCTCGGTGCAGC TACACCTACAGGCAACAACAAAAGACGGAAA CTCTGTAGTGGTAACACTACGCCTATAATACA TTTAAAAGGTGACAGAAACAGTTTAAAATGT TTACGGTACAGATTGCGAAAACATAGCGACC ACTATAGAGATATATCATCCACCTGGCATTGG ACAGGTGCAGGCAATGAAAAAACAGGAATA CTGACTGTAACATACCATAGTGAAACACAAA GAACAAAATTTTTAAATACTGTTGCAATTCCA GATAGTGTACAAATATTGGTGGGATACATGA CAATGTAA ATGACTCTATGTGCAGTACCAGTGACGACAC GGTATCCGCTACTCAGCTTGTTAAACAGCTAC AGCACACCCCCTCACCGTATTCCAGCACCGTG TCCGTGGGCACCGCAAAGACCTACGGCCAGA CGTCGGCTGCTACACGACCTGGACACTGTGG ACTCGCGGAGAAGCAGCATTGTGGACCTGTC AACCCACTTCTCGGTGCAGCTACACCTACAGG CAACAACAAAAGACGGAAACTCTGTAGTGGT AACACTACGCCTATAA ATGTTATCACTTATTTTTTTATTTTGCTTTTGTG TATGCATGTATGTGTGCTGCCATGTCCCGCTT TTGCCATCTGTCTGTATGTGTGCGTATGCATG GGTATTGGTATTTGTGTATATTGTGGTAATAA CGTCCCCTGCCACAGCATTCACAGTATATGTA TTTTGTTTTTTATTGCCCATGTTACTATTGCAT ATACATGCTATATTGTCTTTACAGTAA ATGGCGCGCTTTGAGGATCCAACACGGCGAC CCTACAAGCTACCTGATCTGTGCACGGAACT GAACACTTCACTGCAAGACATAGAAATAACC TGTGTATATTGCAAGACAGTATTGGAACTTAC AGAGGTATTTGAATTTGCATTTAAAGATTTAT TTGTGGTGTATAGAGACAGTATACCGCATGC TGCATGCCATAAATGTATAGATTTTTATTCTA GAATTAGAGAATTAAGACATTATTCAGACTCT GTGTATGGAGACACATTGGAAAAACTAACTA ACACTGGGTTATACAATTTATTAATAAGGTGC CTGCGGTGCCAGAAACCGTTGAATCCAGCAG AAAAACTTAGACACCTTAATGAAAAACGACG ATTTCACAACATAGCTGGGCACTATAGAGGC CAGTGCCATTCGTGCTGCAACCGAGCACGAC AGGAACGACTCCAACGACGCAGAGAAACAC AAGTATAA ATGCAGACCCCTAAGGAAACACTGAGCGAAC GACTGTCATGCGTCCAGGATAAAATCATCGA CCATTACGAAAACGACTCCAAAGATATCGAC AGCCAGATTCAGTACTGGCAGCTGATCCGGT GGGAGAACGCAATTTTCTTTGCCGCTAGAGA ACACGGAATCCAGACCCTGAACCATCAGGTG GTCCCCGCCTACAATATCTCAAAGAGCAAAG CCCACAAGGCTATTGAGCTGCAAATGGCACT CCAGGGCCTGGCCCAGTCCCGATATAAAACA GAGGACTGGACTCTCCAGGATACCTGCGAGG AACTGTGGAATACAGAACCTACTCATTGTTTC AAGAAAGGCGGGCAGACCGTGCAGGTCTAC TTTGACGGGAACAAGGATAATTGCATGACCT ACGTGGCCTGGGATTCCGTCTACTATATGAC AGACGCTGGAACTTGGGATAAGACTGCAACC TGTGTGTCTCACAGGGGCCTGTACTATGTGA AAGAGGGGTACAACACCTTCTATATCGAGTT CAAGTCTGAGTGCGAAAAATACGGGAATACA GGAACTTGGGAGGTGCACTTCGGGAACAAT GTCATTGACTGCAACGATAGCATGTGTTCCAC CTCTGACGATACAGTGTCCGCCACTCAGCTG GTCAAGCAGCTCCAGCATACACCCAGTCCTTA CAGCTCCACTGTGTCAGTCGGAACCGCCAAA ACCTACGGCCAGACCAGTGCAGCCACACGGC CAGGCCACTGCGGACTGGCTGAAAAGCAGC ATTGTGGCCCAGTGAATCCCCTGCTGGGGGC TGCAACCCCTACAGGAAACAATAAGCGGAGA AAACTGTGCAGCGGAAACACCACACCAATCA TTCACCTGAAGGGCGACCGGAACAGCCTGAA GTGTCTGCGGTACAGACTGCGAAAGCACAGT GACCATTATCGCGATATCTCTAGTACTTGGCA CTGGACCGGAGCTGGCAACGAGAAGACCGG CATTCTGACTGTGACCTACCATTCAGAAACTC AGCGGACCAAATTTCTGAATACTGTGGCCAT CCCCGATAGCGTGCAGATTCTGGTCGGGTAT ATGACAATG ATGACCCTGTGTGCTGTCCCTGTGACTACAAG ATACCCCCTGCTGTCCCTGCTGAACTCCTACT CCACCCCTCCCCATAGAATCCCCGCACCATGC CCTTGGGCTCCACAGAGACCAACTGCACGGA GAAGGCTGCTGCACGACCTGGATACCGTGGA CAGCCGGCGGAGCAGCATCGTGGATCTGTCT ACACACTTCAGTGTCCAGCTGCACCTCCAGGC CACCACAAAGGACGGCAACTCTGTGGTCGTG ACCCTGCGGCTG ATGCTGAGTCTGATTTTCCTGTTTTGTTTCTGC GTGTGTATGTATGTCTGCTGTCATGTCCCACT GCTGCCTTCTGTCTGTATGTGTGCCTACGCTT GGGTGCTGGTCTTCGTGTATATCGTGGTCATT ACCAGCCCCGCAACCGCCTTTACAGTCTACGT GTTCTGCTTTCTGCTGCCTATGCTGCTGCTGC ACATCCATGCTATTCTGAGCCTCCAG ATGGCTAGATTTGAGGACCCAACAAGACGCC CCTATAAACTGCCCGACCTGTGTACCGAACTG AATACTTCTCTGCAGGACATTGAGATCACATG CGTGTACTGTAAAACTGTCCTGGAGCTGACC GAAGTGTTCGAGTTTGCTTTCAAGGACCTGTT TGTGGTCTACAGAGATTCTATTCCCCACGCCG CTTGCCATAAATGTATCGACTTCTATAGTCGG ATTAGAGAACTGAGGCACTACAGCGACTCCG TCTATGGAGATACTCTGGAGAAGCTGACCAA CACAGGCCTGTACAATCTGCTGATCCGATGCC TGAGGTGTCAGAAGCCCCTGAACCCTGCCGA AAAACTGCGGCACCTGAACGAGAAGCGGAG ATTTCACAATATTGCAGGCCATTATCGCGGGC AGTGCCATTCCTGCTGTAATAGGGCCCGCCA GGAACGACTCCAGAGGCGCCGAGAGACCCA GGTG MQTPKETLSERLSALQDKIIDHYEND SKDIDSQIQYWQLIRWENAIFFAARE HGIQTLNHQVVPAYNISKSKAHKAIE LQMALQGLAQSAYKTEDWTLQDTC EELWNTEPTHCFKKGGQTVQVYFD GNKDNCMTYVAWDSVYYMTDAGT WDKTATCVSHRGLYYVKEGYNTFYIE FKSECEKYGNTGTWEVHFGNNVIDC NDSMCSTSDDTVSATQLVKQLQHT PSPYSSTVSVGTAKTYGQTSAATRPG HCGLAEKQHCGPVNPLLGAATPTG NNKRRKLCSGNTTPIIHLKGDRNSLK CLRYRLRKHSDHYRDISSTWHWTGA GNEKTGILTVTYHSETQRTKFLNTVAI PDSVQILVGYMTM HPV18 E4 HPV18 E5 HPV18 E6 Page 14 of 48 % Nucleotide Change 25.2% MTLCAVPVTTRYPLLSLLNSYSTPPH RIPAPCPWAPQRPTARRRLLHDLDT VDSRRSSIVDLSTHFSVQLHLQATTK DGNSVVVTLRL 27.6% MLSLIFLFCFCVCMYVCCHVPLLPSV CMCAYAWVLVFVYIVVITSPATAFTV YVFCFLLPMLLLHIHAILSLQ 29.2% MARFEDPTRRPYKLPDLCTELNTSLQ DIEITCVYCKTVLELTEVFEFAFKDLFV VYRDSIPHAACHKCIDFYSRIRELRHY SDSVYGDTLEKLTNTGLYNLLIRCLRC QKPLNPAEKLRHLNEKRRFHNIAGH YRGQCHSCCNRARQERLQRRRETQ V 27.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV18 E7 ATGCATGGACCTAAGGCAACATTGCAAGACA TTGTATTGCATTTAGAGCCCCAAAATGAAATT CCGGTTGACCTTCTATGTCACGAGCAATTAAG CGACTCAGAGGAAGAAAACGATGAAATAGA TGGAGTTAATCATCAACATTTACCAGCCCGAC GAGCCGAACCACAACGTCACACAATGTTGTG TATGTGTTGTAAGTGTGAAGCCAGAATTGAG CTAGTAGTAGAAAGCTCAGCAGACGACCTTC GAGCATTCCAGCAGCTGTTTCTGAACACCCTG TCCTTTGTGTGTCCGTGGTGTGCATCCCAGCA GTAA ATGGCTTTGTGGCGGCCTAGTGACAATACCG TATATCTTCCACCTCCTTCTGTGGCAAGAGTT GTAAATACCGATGATTATGTGACTCGCACAA GCATATTTTATCATGCTGGCAGCTCTAGATTA TTAACTGTTGGTAATCCATATTTTAGGGTTCC TGCAGGTGGTGGCAATAAGCAGGATATTCCT AAGGTTTCTGCATACCAATATAGAGTATTTAG GGTGCAGTTACCTGACCCAAATAAATTTGGTT TACCTGATACTAGTATTTATAATCCTGAAACA CAACGTTTAGTGTGGGCCTGTGCTGGAGTGG AAATTGGCCGTGGTCAGCCTTTAGGTGTTGG CCTTAGTGGGCATCCATTTTATAATAAATTAG ATGACACTGAAAGTTCCCATGCCGCCACGTCT AATGTTTCTGAGGACGTTAGGGACAATGTGT CTGTAGATTATAAGCAGACACAGTTATGTATT TTGGGCTGTGCCCCTGCTATTGGGGAACACT GGGCTAAAGGCACTGCTTGTAAATCGCGTCC TTTATCACAGGGCGATTGCCCCCCTTTAGAAC TTAAAAACACAGTTTTGGAAGATGGTGATAT GGTAGATACTGGATATGGTGCCATGGACTTT AGTACATTGCAAGATACTAAATGTGAGGTAC CATTGGATATTTGTCAGTCTATTTGTAAATAT CCTGATTATTTACAAATGTCTGCAGATCCTTA TGGGGATTCCATGTTTTTTTGCTTACGGCGTG AGCAGCTTTTTGCTAGGCATTTTTGGAATAGA GCAGGTACTATGGGTGACACTGTGCCTCAAT CCTTATATATTAAAGGCACAGGTATGCGTGCT TCACCTGGCAGCTGTGTGTATTCTCCCTCTCC AAGTGGCTCTATTGTTACCTCTGACTCCCAGT TGTTTAATAAACCATATTGGTTACATAAGGCA CAGGGTCATAACAATGGTGTTTGCTGGCATA ATCAATTATTTGTTACTGTGGTAGATACCACT CGCAGTACCAATTTAACAATATGTGCTTCTAC ACAGTCTCCTGTACCTGGGCAATATGATGCTA CCAAATTTAAGCAGTATAGCAGACATGTTGA GGAATATGATTTGCAGTTTATTTTTCAGTTGT GTACTATTACTTTAACTGCAGATGTTATGTCC TATATTCATAGTATGAATAGCAGTATTTTAGA GGATTGGAACTTTGGTGTTCCCCCCCCGCCAA CTACTAGTTTGGTGGATACATATCGTTTTGTA CAATCTGTTGCTATTACCTGTCAAAAGGATGC TGCACCGGCTGAAAATAAGGATCCCTATGAT AAGTTAAAGTTTTGGAATGTGGATTTAAAGG AAAAGTTTTCTTTAGACTTAGATCAATATCCC CTTGGACGTAAATTTTTGGTTCAGGCTGGATT GCGTCGCAAGCCCACCATAGGCCCTCGCAAA CGTTCTGCTCCATCTGCCACTACGTCTTCTAA ACCTGCCAAGCGTGTGCGTGTACGTGCCAGG AAGTAA ATGCATGGACCCAAGGCTACTCTGCAGGATA TTGTGCTGCACCTGGAACCTCAGAATGAAAT CCCCGTGGACCTGCTGTGTCACGAACAGCTG TCTGACAGTGAGGAAGAGAACGACGAGATC GATGGCGTGAATCACCAGCATCTGCCCGCCC GGAGAGCTGAACCTCAGAGGCACACCATGCT GTGCATGTGCTGTAAGTGTGAAGCTCGCATT GAGCTGGTGGTCGAAAGCTCCGCAGACGATC TGCGAGCCTTCCAGCAGCTGTTTCTGAACACA CTGAGCTTCGTCTGCCCCTGGTGTGCCTCTCA GCAG ATGTGCCTGTATACCCGCGTGCTGATTCTGCA TTACCATCTGCTGCCCCTGTACGGACCCCTGT ACCATCCAAGACCTCTGCCTCTGCACTCCATT CTGGTGTACATGGTCCACATCATTATCTGCGG GCATTATATTATCCTGTTCCTGCGCAACGTGA ATGTCTTCCCTATCTTCCTCCAGATGGCTCTGT GGCGACCAAGTGACAACACCGTGTACCTGCC CCCTCCATCAGTCGCACGGGTGGTCAATACA GACGATTACGTGACCCCTACAAGCATTTTCTA TCATGCAGGCAGCTCCCGACTGCTGACCGTG GGAAACCCTTATTTTCGGGTCCCAGCAGGCG GGGGAAATAAGCAGGATATCCCAAAAGTGTC TGCCTACCAGTATCGGGTGTTCAGAGTCCAG CTGCCCGACCCTAACAAGTTTGGCCTGCCAG ATACTAGCATCTACAATCCCGAGACCCAGAG ACTGGTGTGGGCTTGTGCAGGAGTCGAAATC GGAAGGGGACAGCCACTGGGAGTGGGACTG TCTGGACACCCTTTCTACAACAAGCTGGACGA TACAGAGTCTAGTCATGCCGCTACTTCAAACG TGAGCGAAGACGTCAGAGATAATGTGAGCG TGGACTATAAACAGACTCAGCTGTGCATTCTG GGATGTGCACCAGCTATCGGAGAGCACTGG GCTAAGGGAACCGCATGCAAATCTAGGCCTC TGAGTCAGGGCGACTGTCCCCCTCTGGAGCT GAAGAATACCGTGCTGGAAGACGGGGATAT GGTCGATACAGGGTACGGAGCTATGGACTTT TCTACACTCCAGGATACTAAGTGCGAGGTGC CTCTGGACATTTGCCAGAGTATCTGTAAATAC CCAGATTACCTCCAGATGTCCGCTGACCCCTA TGGCGATTCTATGTTCTTTTGTCTGCGGAGAG AACAGCTGTTCGCCAGGCACTTTTGGAACCG CGCTGGCACAATGGGCGACACAGTGCCCCAG AGCCTGTACATTAAGGGCACAGGAATGCCAG CATCCCCTGGATCTTGCGTGTATAGTCCATCA CCCAGCGGCTCCATCGTGACCTCTGACAGTC AGCTGTTCAATAAGCCATACTGGCTGCACAA AGCTCAGGGGCATAACAATGGAGTGTGCTG GCATAACCAGCTGTTTGTCACAGTGGTCGAT ACCACACCCAGCACTAATCTGACCATCTGTGC CAGTACACAGTCACCTGTGCCAGGACAGTAC GACGCTACTAAGTTCAAACAGTACTCCAGAC ACGTGGAGGAATATGACCTCCAGTTCATCTTT CAGCTGTGCACTATCACCCTGACAGCCGACG TGATGTCATACATTCATAGCATGAACTCAAGC ATCCTGGAGGATTGGAATTTCGGCGTGCCAC CCCCTCCAACTACCAGCCTGGTGGACACTTAT CGCTTTGTGCAGTCCGTCGCTATTACCTGTCA GAAGGATGCAGCCCCCGCAGAGAACAAAGA CCCTTACGATAAGCTGAAATTCTGGAATGTG GACCTGAAGGAAAAATTTTCCCTGGACCTGG ATCAGTATCCCCTGGGACGGAAGTTTCTGGT GCAGGCAGGACTGAGGCGAAAGCCAACCAT CGGACCACGAAAACGGAGCGCACCTTCCGCC ACAACTTCCTCTAAGCCAGCAAAAAGAGTGA GGGTCCGCGCCCGAAAA MHGPKATLQDIVLHLEPQNEIPVDLL CHEQLSDSEEENDEIDGVNHQHLPA RRAEPQRHTMLCMCCKCEARIELVV ESSADDLRAFQQLFLNTLSFVCPWC ASQQ HPV18 L1 Page 15 of 48 MALWRPSDNTVYLPPPSVARVVNT DDYVTRTSIFYHAGSSRLLTVGNPYF RVPAGGGNKQDIPKVSAYQYRVFRV QLPDPNKFGLPDTSIYNPETQRLVW ACAGVEIGRGQPLGVGLSGHPFYNK LDDTESSHAATSNVSEDVRDNVSVD YKQTQLCILGCAPAIGEHWAKGTAC KSRPLSQGDCPPLELKNTVLEDGDM VDTGYGAMDFSTLQDTKCEVPLDIC QSICKYPDYLQMSADPYGDSMFFCL RREQLFARHFWNRAGTMGDTVPQ SLYIKGTGMRASPGSCVYSPSPSGSIV TSDSQLFNKPYWLHKAQGHNNGVC WHNQLFVTVVDTTRSTNLTICASTQ SPVPGQYDATKFKQYSRHVEEYDLQ FIFQLCTITLTADVMSYIHSMNSSILE DWNFGVPPPPTTSLVDTYRFVQSVA ITCQKDAAPAENKDPYDKLKFWNVD LKEKFSLDLDQYPLGRKFLVQAGLRR KPTIGPRKRSAPSATTSSKPAKRVRV RARK % Nucleotide Change 23.8% 34.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV18 L2 ATGGTATCCCACCGTGCCGCACGACGCAAAC GGGCTTCGGTAACTGACTTATATAAAACATGT AAACAATCTGGTACATGTCCACCTGATGTTGT TCCTAAGGTGGAGGGCACCACGTTAGCAGAT AAAATATTGCAATGGTCAAGCCTTGGTATATT TTTGGGTGGACTTGGCATAGGTACTGGCAGT GGTACAGGGGGTCGTACAGGGTACATTCCAT TGGGTGGGCGTTCCAATACAGTGGTGGATGT TGGTCCTACACGTCCCCCAGTGGTTATTGAAC CTGTGGGCCCCACAGACCCATCTATTGTTACA TTAATAGAGGACTCCAGTGTGGTTACATCAG GTGCACCTAGGCCTACGTTTACTGGCACGTCT GGGTTTGATATAACATCTGCGGGTACAACTA CACCTGCGGTTTTGGATATCACACCTTCGTCT ACCTCTGTGTCTATTTCCACAACCAATTTTACC AATCCTGCATTTTCTGATCCGTCCATTATTGA AGTTCCACAAACTGGGGAGGTGGCAGGTAAT GTATTTGTTGGTACCCCTACATCTGGAACACA TGGGTATGAGGAAATACCTTTACAAACATTT GCTTCTTCTGGTACGGGGGAGGAACCCATTA GTAGTACCCCATTGCCTACTGTGCGGCGTGT AGCAGGTCCCCGCCTTTACAGTAGGGCCTAC CAACAAGTGTCAGTGGCTAACCCTGAGTTTCT TACACGTCCATCCTCTTTAATTACATATGACA ACCCGGCCTTTGAGCCTGTGGACACTACATTA ACATTTGATCCTCGTAGTGATGTTCCTGATTC AGATTTTATGGATATTATCCGTCTACATAGGC CTGCTTTAACATCCAGGCGTGGGACTGTTCG CTTTAGTAGATTAGGTCAACGGGCAACTATG TTTACCCGCAGCGGTACACAAATAGGTGCTA GGGTTCACTTTTATCATGATATAAGTCCTATT GCACCTTCCCCAGAATATATTGAACTGCAGCC TTTAGTATCTGCCACGGAGGACAATGACTTG TTTGATATATATGCAGATGACATGGACCCTGC AGTGCCTGTACCATCGCGTTCTACTACCTCCT TTGCATTTTTTAAATATTCGCCCACTATATCTT CTGCCTCTTCCTATAGTAATGTAACGGTCCCT TTAACCTCCTCTTGGGATGTGCCTGTATACAC GGGTCCTGATATTACATTACCATCTACTACCT CTGTATGGCCCATTGTATCACCCACGGCCCCT GCCTCTACACAGTATATTGGTATACATGGTAC ACATTATTATTTGTGGCCATTATATTATTTTAT TCCTAAGAAACGTAAACGTGTTCCCTATTTTT TTGCAGATGGCTTTGTGGCGGCCTAG ATGGTGTCTCATCGCGCAGCACGACGGAAAA GAGCCAGTGTGACCGACCTGTATAAAACCTG TAAGCAGAGCGGAACTTGCCCTCCTGACGTG GTCCCCAAGGTGGAGGGAACCACACTGGCTG ATAAGATCCTCCAGTGGAGCAGCCTGGGAAT CTTCCTGGGAGGACTGGGAATTGGGACTGG AAGCGGCACCGGAGGCCGAACAGGCTACAT CCCTCTGGGGGGACGCAGCAACACCGTGGT GGACGTGGGACCAACAAGGCCCCCTGTGGTC ATTGAGCCTGTGGGGCCAACTGACCCCTCCA TCGTCACCCTGATTGAAGATTCTAGTGTGGTC ACATCTGGGGCCCCACGACCAACATTCACTG GCACCTCCGGGTTTGACATCACCTCTGCTGGA ACTACCACACCCGCCGTGCTGGACATCACTCC ATCAAGCACCAGTGTGTCAATTAGCACTACCA ACTTCACAAATCCAGCCTTTAGTGATCCCTCA ATCATTGAGGTGCCCCAGACTGGCGAAGTCG CTGGGAATGTGTTCGTCGGCACACCCACTAG CGGAACCCACGGCTACGAGGAAATCCCTCTC CAGACATTTGCATCCTCTGGGACTGGAGAGG AACCAATTAGTTCAACACCTCTGCCAACTGTG CGGAGAGTCGCAGGACCACGACTGTACAGC AGAGCATATCAGCAGGTGTCCGTCGCCAACC CCGAGTTCCTGACTCGGCCTAGCTCCCTGATC ACCTATGACAATCCCGCTTTCGAACCTGTGGA TACAACTCTGACCTTTGACCCTCGGAGCGATG TGCCAGACAGTGATTTTATGGACATCATTAGA CTGCATAGGCCAGCACTGACTAGCAGGCGCG GGACCGTGCGCTTCAGCCGACTGGGACAGA GGGCCACCATGTTTACACGCTCCGGAACACA GATTGGCGCTAGGGTGCACTTCTACCATGAT ATCTCACCAATTGCACCCAGCCCTGAGTATAT CGAGCTGCAACCCCTGGTGTCTGCCACCGAG GACAACGATCTGTTCGACATCTACGCAGACG ATATGGACCCCGCCGTGCCCGTGCCCAGCCG GAGCACCACATCTTTTGCTTTCTTTAAGTACA GTCCCACCATCTCTAGTGCATCAAGCTATAGC AATGTGACCGTCCCTCTGACATCCTCTTGGGA CGTGCCCGTCTATACAGGCCCTGATATCACTC TGCCAAGCACTACCTCCGTGTGGCCTATTGTC AGTCCTACCGCACCAGCCTCAACACAGTACAT CGGGATTCACGGAACCCATTACTATCTGTGG CCACTGTACTATTTCATCCCCAAGAAACGAAA ACGGGTGCCATATTTCTTTGCCGACGGCTTTG TGGCCGCT MVSHRAARRKRASVTDLYKTCKQSG TCPPDVVPKVEGTTLADKILQWSSLG IFLGGLGIGTGSGTGGRTGYIPLGGR SNTVVDVGPTRPPVVIEPVGPTDPSI VTLIEDSSVVTSGAPRPTFTGTSGFDI TSAGTTTPAVLDITPSSTSVSISTTNFT NPAFSDPSIIEVPQTGEVAGNVFVGT PTSGTHGYEEIPLQTFASSGTGEEPIS STPLPTVRRVAGPRLYSRAYQQVSVA NPEFLTRPSSLITYDNPAFEPVDTTLT FDPRSDVPDSDFMDIIRLHRPALTSR RGTVRFSRLGQRATMFTRSGTQIGA RVHFYHDISPIAPSPEYIELQPLVSATE DNDLFDIYADDMDPAVPVPSRSTTS FAFFKYSPTISSASSYSNVTVPLTSSW DVPVYTGPDITLPSTTSVWPIVSPTA PASTQYIGIHGTHYYLWPLYYFIPKKR KRVPYFFADGFVAA Page 16 of 48 % Nucleotide Change 27.2% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV31 E1 ATGGCTGATCCAGCAGGTACAGATGGGGAG GGGACGGGATGCAATGGTTGGTTTTATGTAG AAGCAGTAATTGACAGACAGACAGGGGACA ACATTTCAGAGGACGAAAATGAAGACAGTAG TGATACTGGGGAGGATATGGTTGACTTTATT GACAATTGTAATGTATACAACAATCAGGCAG AAGCAGAGACAGCACAGGCATTGTTTCATGC ACAGGAAGCGGAGGAACATGCAGAGGCTGT GCAGGTTCTAAAACGAAAGTATGTAGGTAGT CCTTTAAGTGATATTAGTAGTTGTGTGGATTA TAATATTAGTCCACGGTTAAAAGCTATATGCA TAGAAAATAACAGTAAAACAGCAAAACGAAG ACTCTTTGAACTTCCAGACAGCGGGTATGGC AATACTGAAGTGGAAACGCAGCAGATGGTAC AGGTAGAGGAGCAACAAACAACATTAAGTTG TAATGGTAGTGACGGGACACATAGTGAACGA GAGAATGAAACTCCAACACGTAATATATTGC AAGTGTTAAAAACTAGCAATGGTAAAGCTGC TATGTTAGGTAAATTTAAAGAATTATATGGTG TAAGTTTTATGGAACTAATTAGGCCATTTCAA AGCAATAAAAGCACATGTACTGATTGGTGTG TAGCTGCGTTTGGAGTTACAGGTACAGTTGC AGAAGGATTTAAAACCCTATTGCAACCATATT GTTTGTATTGCCATTTACAAAGTTTAGCATGT TCCTGGGGCATGGTTATGTTAATGCTTGTGA GATTTAAATGTGCAAAAAATAGAATAACAAT TGAAAAATTATTAGAAAAATTATTGTGTATAT CTACAAATTGTATGTTAATTCAGCCACCCAAA TTACGTAGCACAGCTGCAGCATTATATTGGTA CAGAACAGGAATGTCAAACATTAGCGATGTA TATGGTGAAACACCAGAATGGATAGAAAGAC AAACAGTATTACAGCATAGTTTTAATGACACA ACATTTGATTTGTCCCAAATGGTACAATGGGC ATATGACAATGATGTTATGGATGATAGTGAA ATTGCCTATAAATATGCACAATTAGCTGACAG TGATAGTAATGCATGTGCATTTTTAAAAAGTA ATTCGCAGGCAAAAATAGTTAAAGATTGTGG AACAATGTGTAGACATTATAAACGAGCAGAA AAACGACAAATGTCCATGGGACAGTGGATTA AAAGTAGATGTGACAAAGTTAGTGACGAAG GTGACTGGAGGGACATAGTAAAGTTTTTAAG ATATCAACAAATAGAATTTGTGTCATTTTTAT CTGCATTAAAGCTGTTTTTAAAAGGAGTGCCA AAGAAAAACTGTATTTTAATACATGGTGCACC TAATACAGGTAAATCATATTTTGGAATGAGCC TTATTAGCTTTTTACAAGGATGTATAATATCA TATGCAAATTCAAAAAGTCATTTTTGGTTACA ACCACTGGCTGATGCTAAAATAGGCATGTTA GATGATGCTACAACGCCATGTTGGCATTATAT AGACAATTACCTACGAAATGCACTAGATGGC AACCCTGTATCTATAGATGTAAAGCATAAAG CTTTAATGCAGTTAAAATGTCCTCCTTTATTG ATTACATCTAATATAAATGCAGGTAAGGATG ACAGATGGCCATACCTACATAGCAGACTGGT GGTTTTTACATTTCCAAATCCATTTCCATTTGA CAAAAACGGAAATCCAGTATATGAATTAAGT GATAAAAACTGGAAATCCTTTTTCTCAAGGAC GTGGTGCAGATTAAATTTGCACGAGGAAGAG GACAAAGAAAACGATGGAGACTCTTTCTCAA CGTTTAAATGTGTGTCAGGACAAAATATTAG AACATTATGA ATGGCAGACCCAGCAGGAACAGACGGAGAG GGGACAGGATGTAATGGATGGTTTTATGTGG AGGCAGTGATTGACAGGCAGACCGGCGACA ACATCAGCGAAGATGAGAATGAAGACAGCTC CGATACCGGCGAGGACATGGTGGATTTCATT GACAACTGCAATGTCTACAACAATCAGGCTG AGGCAGAAACAGCCCAGGCTCTGTTTCACGC ACAGGAGGCCGAGGAACATGCAGAAGCCGT GCAGGTCCTGAAGAGAAAATACGTGGGGTCT CCCCTGAGTGACATCTCTAGTTGCGTCGATTA TAACATTTCTCCTAGGCTGAAGGCCATCTGTA TTGAGAACAATAGTAAGACCGCTAAACGGAG ACTGTTCGAACTGCCAGACTCTGGCTACGGG AACACCGAGGTGGAAACACAGCAGATGGTG CAGGTCGAGGAACAGCAGACCACACTGTCAT GCAATGGAAGCGATGGCACACACAGCGAGA GGGAAAACGAGACCCCCACACGCAATATCCT GCAGGTGCTGAAGACTAGCAACGGCAAAGC CGCTATGCTGGGGAAGTTTAAAGAGCTGTAT GGCGTGTCCTTCATGGAACTGATTCGCCCCTT TCAGTCAAATAAGAGCACTTGCACCGACTGG TGTGTGGCAGCCTTCGGGGTGACAGGAACTG TCGCCGAGGGCTTCAAGACACTGCTGCAGCC TTACTGCCTGTATTGTCACCTGCAGTCCCTGG CCTGCTCTTGGGGCATGGTCATGCTGATGCT GGTCCGATTCAAGTGTGCTAAAAACCGGATC ACTATTGAGAAGCTGCTGGAAAAACTGCTGT GCATCTCAACCAATTGTATGCTGATTCAGCCC CCTAAGCTGCGCAGCACAGCTGCAGCCCTGT ACTGGTATCGGACTGGAATGTCCAATATCTCT GATGTGTACGGCGAAACTCCTGAGTGGATTG AACGCCAGACCGTCCTGCAGCACAGCTTCAA CGACACTACCTTTGATCTGTCCCAGATGGTGC AGTGGGCATATGACAATGATGTCATGGACGA TTCTGAGATCGCCTACAAGTATGCTCAGCTG GCAGACTCAGATAGCAACGCTTGCGCATTTCT GAAATCCAATTCTCAGGCAAAGATCGTGAAA GACTGCGGCACAATGTGTAGGCATTACAAGC GCGCCGAGAAACGACAGATGAGCATGGGCC AGTGGATTAAGAGTCGGTGTGATAAAGTGTC AGATGAGGGGGACTGGAGAGATATCGTCAA GTTCCTGAGGTATCAGCAGATTGAATTCGTG AGTTTTCTGTCAGCTCTGAAGCTGTTTCTGAA AGGCGTGCCTAAGAAAAACTGCATCCTGATT CACGGCGCCCCAAATACTGGGAAGAGTTACT TCGGAATGAGCCTGATCTCCTTTCTGCAGGG GTGTATCATTAGCTATGCCAACAGTAAGTCAC ATTTCTGGCTGCAGCCACTGGCCGACGCTAA AATCGGAATGCTGGACGATGCCACAACTCCC TGCTGGCACTACATTGATAACTATCTGAGAAA TGCTCTGGACGGCAATCCCGTGTCCATCGAT GTCAAGCATAAAGCACTGATGCAGCTGAAGT GTCCACCCCTGCTGATCACTTCCAACATTAAT GCCGGGAAAGACGATCGCTGGCCTTACCTGC ACTCTCGACTGGTGGTCTTCACCTTTCCTAAC CCATTCCCCTTTGACAAGAACGGAAATCCAGT GTATGAGCTGAGCGATAAGAATTGGAAATCT TTCTTTAGTCGGACCTGGTGCAGACTGAACCT GCATGAGGAAGAGGACAAGGAGAATGACGG GGATAGCTTCTCCACCTTTAAATGTGTGTCCG GACAGAACATCAGGACACTG MADPAGTDGEGTGCNGWFYVEAVI DRQTGDNISEDENEDSSDTGEDMV DFIDNCNVYNNQAEAETAQALFHA QEAEEHAEAVQVLKRKYVGSPLSDIS SCVDYNISPRLKAICIENNSKTAKRRL FELPDSGYGNTEVETQQMVQVEEQ QTTLSCNGSDGTHSERENETPTRNIL QVLKTSNGKAAMLGKFKELYGVSFM ELIRPFQSNKSTCTDWCVAAFGVTG TVAEGFKTLLQPYCLYCHLQSLACSW GMVMLMLVRFKCAKNRITIEKLLEKL LCISTNCMLIQPPKLRSTAAALYWYR TGMSNISDVYGETPEWIERQTVLQH SFNDTTFDLSQMVQWAYDNDVMD DSEIAYKYAQLADSDSNACAFLKSNS QAKIVKDCGTMCRHYKRAEKRQMS MGQWIKSRCDKVSDEGDWRDIVKF LRYQQIEFVSFLSALKLFLKGVPKKNCI LIHGAPNTGKSYFGMSLISFLQGCIIS YANSKSHFWLQPLADAKIGMLDDA TTPCWHYIDNYLRNALDGNPVSIDV KHKALMQLKCPPLLITSNINAGKDDR WPYLHSRLVVFTFPNPFPFDKNGNP VYELSDKNWKSFFSRTWCRLNLHEE EDKENDGDSFSTFKCVSGQNIRTL Page 17 of 48 % Nucleotide Change 26.2% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV31 E2 ATGGAGACTCTTTCTCAACGTTTAAATGTGTG TCAGGACAAAATATTAGAACATTATGAAAAT GATAGTAAACGACTTTGTGATCATATAGACTA TTGGAAACATATTCGACTTGAATGTGTATTAA TGTATAAAGCAAGAGAAATGGGAATACACA GTATTAACCACCAGGTGGTGCCAGCGTTGTC AGTATCAAAGGCCAAAGCCTTACAAGCTATT GAACTACAAATGATGTTGGAAACATTAAATA ACACTGAATACAAAAATGAGGACTGGACAAT GCAGCAAACAAGTCTTGAACTGTATTTAACTG CACCTACAGGGTGTTTAAAAAAACATGGATA TACTGTAGAGGTGCAATTTGATGGTGATGTA CACAACACCATGCATTATACTAACTGGAAATT TATATACCTATGTATAGATGGCCAATGTACTG TTGTGGAAGGGCAAGTTAATTGTAAGGGCAT TTATTATGTACATGAAGGACATATAACATATT TTGTAAATTTTACAGAAGAGGCAAAAAAATA TGGGACTGGTAAAAAATGGGAAGTGCATGC GGGTGGTCAGGTAATTGTTTTTCCTGAATCTG TATTTAGCAGTGACGAAATATCCTTTGCTGGG ATTGTTACAAAGCTACCAACAGCCAACAACA CCACCACATCGAATTCCAAAACCTGCGCCTTG GGCACCAGTGAAGGTGTGCGGCGGGCGACG ACGTCTACTAAGCGACCAAGAACAGAGCCAG AGCACAGAAACACCCACCACCCCAACAAGTT GTTGCGAGGCGACTCCGTGGACAGTGTCAAC TGTGGGGTTATCAGTGCAGCTGCATGCACAA ACCAAACAAGGGCTGTCAGTTGTCCTGCAAC TACACCTATAATACACTTAAAAGGTGATGCAA ATATATTAAAATGTTTAAGATATAGGCTGTCA AAATATAAACAATTGTATGAACAAGTGTCATC TACATGGCATTGGACATGTACAGATGGAAAA CATAAAAATGCTATTGTAACCTTAACATATAT AAGTACATCACAAAGAGACGATTTTTTAAATA CTGTAAAAATACCTAACACAGTATCAGTGTCA ACAGGATATATGACTATTTAG TTGTTTTTCCTGAATCTGTATTTAGCAGTGAC GAAATATCCTTTGCTGGGATTGTTACAAAGCT ACCAACAGCCAACAACACCACCACATCGAAT TCCAAAACCTGCGCCTTGGGCACCAGTGAAG GTGTGCGGCGGGCGACGACGTCTACTAAGC GACCAAGAACAGAGCCAGAGCACAGAAACA CCCACCACCCCAACAAGTTGTTGCGAGGCGA CTCCGTGGACAGTGTCAACTGTGGGGTTATC AGTGCAGCTGCATGCACAAACCAAACAAGGG CTGTCAGTTGTCCTGCAACTACACCTATAA ATGATTGAACTAAATATTTCTACAGTAAGCAT TGTGCTATGCTTTTTGCTTTGCTTTTGTGTGCT ACTATTTGTGTGTCTTGTCATACGTCCACTTGT GCTGTCTGTGTCGGTATATGCAACACTACTAT TATTAATTGTGATTTTATGGGTTATTGCAACC TCTCCATTACGTTGTTTTTGTATATATGTTGTG TTTATATATATTCCATTATTTGTAATTCATACA CATGCATCTTTTTTAAGTCAACAGTAA ATGTTCAAAAATCCTGCAGAAAGACCTCGGA AATTGCATGAACTAAGCTCGGCATTGGAAAT ACCCTACGATGAACTAAGATTGAATTGTGTCT ACTGCAAAGGTCAGTTAACAGAAACAGAGGT ATTAGATTTTGCATTTACAGATTTAACAATAG TATATAGGGACGACACACCACACGGAGTGTG TACAAAATGTTTAAGATTTTATTCAAAAGTAA GTGAATTTAGATGGTATAGATATAGTGTGTA TGGAACAACATTAGAAAAATTGACAAACAAA GGTATATGTGATTTGTTAATTAGGTGTATAAC GTGTCAAAGACCGTTGTGTCCAGAAGAAAAA CAAAGACATTTGGATAAAAAGAAACGATTCC ACAACATAGGAGGAAGGTGGACAGGACGTT GCATAGCATGTTGGAGAAGACCTCGTACTGA AACCCAAGTGTAA ATGGAAACTCTGTCCCAGCGACTGAACGTCT GCCAGGATAAGATTCTGGAACACTACGAAAA TGATTCTAAAAGACTGTGCGACCACATCGACT ACTGGAAGCACATTAGACTGGAGTGCGTGCT GATGTATAAAGCCAGGGAAATGGGCATCCAC AGCATTAACCATCAGGTGGTCCCCGCACTGTC AGTGAGCAAGGCCAAAGCTCTGCAGGCCATC GAGCTGCAGATGATGCTGGAAACCCTGAACA ATACAGAGTACAAGAATGAAGACTGGACTAT GCAGCAGACCTCCCTGGAGCTGTACCTGACT GCCCCTACCGGCTGCCTGAAGAAACACGGGT ATACAGTGGAAGTCCAGTTCGACGGCGATGT GCACAACACAATGCATTACACTAACTGGAAG TTTATCTATCTGTGCATTGATGGGCAGTGTAC CGTGGTCGAGGGACAGGTGAACTGTAAAGG CATCTACTATGTCCACGAAGGACATATCACTT ACTTCGTGAACTTCACCGAGGAAGCTAAGAA ATATGGAACCGGCAAGAAATGGGAGGTCCA TGCAGGCGGGCAGGTCATCGTCTTCCCTGAG TCAGTGTTTAGCTCCGATGAAATCAGCTTCGC TGGCATTGTCACCAAGCTGCCAACAGCAAAC AATACCACAACTTCCAACTCTAAAACATGCGC ACTGGGAACTTCCGAGGGAGTGCGGAGAGC TACCACATCTACCAAGAGGCCCCGCACAGAG CCTGAACACCGCAACACCCACCATCCAAACA AGCTGCTGCGAGGGGACTCTGTGGATAGTGT CAACTGCGGAGTGATCAGTGCCGCTGCATGT ACAAATCAGACTAGGGCAGTCAGCTGCCCAG CCACTACCCCCATCATTCATCTGAAGGGCGAC GCTAACATTCTGAAATGTCTGCGATACCGGCT GTCTAAGTACAAACAGCTGTATGAGCAGGTG TCTAGTACATGGCACTGGACATGTACTGATG GGAAGCATAAAAATGCCATCGTGACCCTGAC ATACATTAGTACCTCACAGCGGGACGATTTTC TGAACACAGTGAAGATCCCCAATACTGTGAG CGTCTCCACTGGCTATATGACCATT ATGTTTTTCCTGAACCTGTATCTGGCCGTGAC AAAGTATCCTCTGCTGGGCCTGCTGCAGTCTT ATCAGCAGCCTACCACCCCCCCTCACCGAATC CCAAAGCCTGCACCATGGGCTCCAGTGAAAG TCTGCGGAGGACGAAGAAGGCTGCTGTCAG ACCAGGAGCAGAGCCAGTCCACTGAAACCCC CACCACACCTACAAGCTGCTGTGAGGCAACA CCCTGGACTGTGTCTACCGTCGGACTGAGTG TGCAGCTGCACGCCCAGACCAAGCAGGGCCT GTCTGTGGTCCTGCAGCTGCATCTG ATGATTGAACTGAATATCTCTACCGTGTCCAT TGTCCTGTGTTTTCTGCTGTGTTTCTGCGTCCT GCTGTTTGTCTGCCTGGTCATCCGGCCCCTGG TGCTGAGCGTGTCCGTCTACGCCACCCTGCTG CTGCTGATCGTGATTCTGTGGGTCATCGCTAC ATCCCCCCTGAGATGCTTCTGTATCTACGTGG TCTTTATCTATATTCCTCTGTTCGTGATCCACA CCCATGCCTCTTTTCTGAGTCAGCAG ATGTTTAAGAACCCCGCCGAGAGACCAAGAA AGCTGCACGAACTGTCATCCGCCCTGGAAAT CCCCTACGACGAACTGAGGCTGAACTGCGTG TACTGTAAAGGGCAGCTGACTGAGACCGAAG TCCTGGACTTCGCCTTTACCGATCTGACAATC GTGTATAGGGACGATACTCCACACGGAGTCT GCACCAAATGTCTGCGGTTCTACAGCAAGGT GTCCGAGTTTAGGTGGTACCGCTATTCTGTCT ATGGAACCACACTGGAAAAACTGACAAACAA GGGCATTTGCGACCTGCTGATCAGATGCATT ACTTGTCAGAGGCCCCTGTGTCCTGAGGAAA AGCAGCGCCACCTGGATAAGAAAAAGCGATT CCATAATATCGGAGGACGATGGACCGGACG ATGCATTGCTTGTTGGCGGAGACCCCGGACA GAGACTCAGGTG METLSQRLNVCQDKILEHYENDSKRL CDHIDYWKHIRLECVLMYKAREMGI HSINHQVVPALSVSKAKALQAIELQ MMLETLNNTEYKNEDWTMQQTSL ELYLTAPTGCLKKHGYTVEVQFDGD VHNTMHYTNWKFIYLCIDGQCTVVE GQVNCKGIYYVHEGHITYFVNFTEEA KKYGTGKKWEVHAGGQVIVFPESVF SSDEISFAGIVTKLPTANNTTTSNSKT CALGTSEGVRRATTSTKRPRTEPEHR NTHHPNKLLRGDSVDSVNCGVISAA ACTNQTRAVSCPATTPIIHLKGDANIL KCLRYRLSKYKQLYEQVSSTWHWTC TDGKHKNAIVTLTYISTSQRDDFLNT VKIPNTVSVSTGYMTI HPV31 E4 HPV31 E5 HPV31 E6 Page 18 of 48 % Nucleotide Change 24.7% LFFLNLYLAVTKYPLLGLLQSYQQPTT PPHRIPKPAPWAPVKVCGGRRRLLS DQEQSQSTETPTTPTSCCEATPWTV STVGLSVQLHAQTKQGLSVVLQLHL 26.8% MIELNISTVSIVLCFLLCFCVLLFVCLVI RPLVLSVSVYATLLLLIVILWVIATSPL RCFCIYVVFIYIPLFVIHTHASFLSQQ 28.6% MFKNPAERPRKLHELSSALEIPYDELR LNCVYCKGQLTETEVLDFAFTDLTIVY RDDTPHGVCTKCLRFYSKVSEFRWY RYSVYGTTLEKLTNKGICDLLIRCITCQ RPLCPEEKQRHLDKKKRFHNIGGRW TGRCIACWRRPRTETQV 27.5% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV31 E7 ATGCGTGGAGAAACACCTACGTTGCAAGACT ATGTGTTAGATTTGCAACCTGAGGCAACTGA CCTCCACTGTTATGAGCAATTACCCGACAGCT CAGATGAGGAGGATGTCATAGACAGTCCAGC TGGACAAGCAGAACCGGACACATCCAATTAC AATATCGTTACCTTTTGTTGTCAGTGTAAGTC TACACTTCGTTTGTGTGTACAGAGCACACAAG TAGATATTCGCATATTGCAAGAGCTGTTAATG GGCTCATTTGGAATCGTGTGCCCCAACTGTTC TACTAGACTGTAA ATGTCTCTGTGGCGGCCTAGCGAGGCTACTG TCTACTTACCACCTGTCCCAGTGTCTAAAGTT GTAAGCACGGATGAATATGTAACACGAACCA ACATATATTATCACGCAGGCAGTGCTAGGCT GCTTACAGTAGGCCATCCATATTATTCCATAC CTAAATCTGACAATCCTAAAAAAATAGTTGTA CCAAAGGTGTCAGGATTACAATATAGGGTAT TTAGGGTTCGTTTACCAGATCCAAACAAATTT GGATTTCCTGATACATCTTTTTATAATCCTGA AACTCAACGCTTAGTTTGGGCCTGTGTTGGTT TAGAGGTAGGTCGCGGGCAGCCATTAGGTG TAGGTATTAGTGGTCATCCATTATTAAATAAA TTTGATGACACTGAAAACTCTAATAGATATGC CGGTGGTCCTGGCACTGATAATAGGGAATGT ATATCAATGGATTATAAACAAACACAACTGT GTTTACTTGGTTGCAAACCACCTATTGGAGA GCATTGGGGTAAAGGTAGTCCTTGTAGTAAC AATGCTATTACCCCTGGTGATTGTCCTCCATT AGAATTAAAAAATTCAGTTATACAAGATGGG GATATGGTTGATACAGGCTTTGGAGCTATGG ATTTTACTGCTTTACAAGACACTAAAAGTAAT GTTCCTTTGGACATTTGTAATTCTATTTGTAAA TATCCAGATTATCTTAAAATGGTTGCTGAGCC ATATGGCGATACATTATTTTTTTATTTACGTA GGGAACAAATGTTTGTAAGGCATTTTTTTAAT AGATCAGGCACGGTTGGTGAATCGGTCCCTA CTGACTTATATATTAAAGGCTCCGGTTCAACA GCTACTTTAGCTAACAGTACATACTTTCCTAC ACCTAGCGGCTCCATGGTTACTTCAGATGCAC AAATTTTTAATAAACCATATTGGATGCAACGT GCTCAGGGACACAATAATGGTATTTGTTGGG GCAATCAGTTATTTGTTACTGTGGTAGATACC ACACGTAGTACCAATATGTCTGTTTGTGCTGC AATTGCAAACAGTGATACTACATTTAAAAGTA GTAATTTTAAAGAGTATTTAAGACATGGTGA GGAATTTGATTTACAATTTATATTTCAGTTAT GCAAAATAACATTATCTGCAGACATAATGAC ATATATTCACAGTATGAATCCTGCTATTTTGG AAGATTGGAATTTTGGATTGACCACACCTCCC TCAGGTTCTTTGGAGGATACCTATAGGTTTGT CACCTCACAGGCCATTACATGTCAAAAAACTG CCCCCCAAAAGCCCAAGGAAGATCCATTTAA AGATTATGTATTTTGGGAGGTTAATTTAAAA GAAAAGTTTTCTGCAGATTTAGATCAGTTTCC ACTGGGTCGCAAATTTTTATTACAGGCAGGA TATAGGGCACGTCCTAAATTTAAAGCAGGTA AACGTAGTGCACCCTCAGCATCTACCACTACA CCAGCAAAACGTAAAAAAACTAAAAAGTAA ATGAGAGGAGAAACCCCAACACTGCAGGACT ATGTGCTGGACCTGCAGCCCGAAGCCACTGA TCTGCATTGCTACGAACAGCTGCCAGACAGC TCCGATGAGGAAGACGTGATCGATTCCCCAG CAGGACAGGCTGAGCCTGACACCAGTAACTA CAATATTGTCACATTCTGCTGTCAGTGCAAGT CTACTCTGCGGCTGTGTGTGCAGAGTACCCA GGTCGATATCAGAATTCTGCAGGAACTGCTG ATGGGCTCATTTGGGATCGTGTGCCCCAACT GTAGCACAAGGCTG ATGTCTCTGTGGCGACCTAGTGAAGCAACTG TCTACCTGCCTCCTGTCCCTGTGTCCAAAGTG GTGTCTACCGACGAGTATGTGACTCGGACTA ATATCTACTATCACGCAGGATCCGCAAGACT GCTGACCGTGGGGCATCCCTACTATTCTATCC CTAAGAGTGACAACCCAAAGAAAATTGTGGT CCCTAAAGTGTCCGGACTGCAGTACAGGGTG TTCAGGGTCCGCCTGCCAGACCCTAATAAGTT CGGCTTTCCCGATACATCTTTTTATAACCCTG AGACTCAGAGGCTGGTGTGGGCATGCGTCG GACTGGAAGTGGGACGAGGACAGCCACTGG GAGTGGGAATTTCAGGACACCCTCTGCTGAA TAAGTTCGACGATACCGAGAACAGCAATCGA TACGCTGGAGGACCAGGAACAGACAACCGA GAATGTATCTCTATGGATTATAAACAGACCCA GCTGTGCCTGCTGGGCTGTAAGCCCCCTATC GGCGAGCATTGGGGCAAAGGGAGCCCTTGC TCCAACAATGCCATTACACCAGGCGACTGTCC ACCCCTGGAACTGAAGAATTCCGTCATCCAG GACGGGGATATGGTGGATACTGGATTCGGC GCTATGGACTTTACCGCACTGCAGGATACAA AAAGTAACGTCCCCCTGGACATCTGCAATTCA ATCTGTAAGTACCCAGATTATCTGAAGATGGT GGCCGAGCCCTACGGGGACACACTGTTCTTT TATCTGCGGAGAGAACAGATGTTCGTGAGAC ACTTCTTTAATAGGTCCGGAACCGTCGGAGA GTCTGTGCCAACAGACCTGTACATTAAGGGG TCTGGAAGTACCGCTACACTGGCAAACTCTAC TTATTTCCCAACCCCCTCAGGCAGCATGGTGA CCAGTGATGCACAGATTTTTAATAAGCCTTAC TGGATGCAGCGGGCCCAGGGACATAACAAT GGCATCTGCTGGGGGAACCAGCTGTTCGTCA CAGTGGTCGACACCACAAGATCAACTAACAT GAGCGTGTGTGCCGCTATCGCCAATAGCGAT ACTACCTTCAAGAGCTCCAACTTTAAAGAGTA CCTGAGACACGGCGAGGAATTTGACCTGCAG TTCATCTTTCAGCTGTGCAAGATTACTCTGAG CGCTGATATCATGACCTATATTCATTCCATGA ACCCAGCAATTCTGGAGGACTGGAATTTCGG GCTGACAACTCCTCCATCCGGATCTCTGGAAG ATACTTACAGGTTTGTGACCAGCCAGGCCATC ACATGTCAGAAGACTGCTCCTCAGAAGCCAA AAGAAGACCCCTTCAAAGATTATGTCTTTTGG GAGGTGAACCTGAAGGAAAAATTCAGTGCC GACCTGGATCAGTTCCCTCTGGGACGCAAGT TTCTGCTGCAGGCAGGATACCGAGCACGACC AAAGTTTAAAGCCGGCAAGCGATCCGCCCCA AGTGCTTCAACCACAACTCCCGCTAAACGCAA GAAAACCAAGAAA MRGETPTLQDYVLDLQPEATDLHCY EQLPDSSDEEDVIDSPAGQAEPDTS NYNIVTFCCQCKSTLRLCVQSTQVDI RILQELLMGSFGIVCPNCSTRL HPV31 L1 Page 19 of 48 MSLWRPSEATVYLPPVPVSKVVSTD EYVTRTNIYYHAGSARLLTVGHPYYSI PKSDNPKKIVVPKVSGLQYRVFRVRL PDPNKFGFPDTSFYNPETQRLVWAC VGLEVGRGQPLGVGISGHPLLNKFD DTENSNRYAGGPGTDNRECISMDYK QTQLCLLGCKPPIGEHWGKGSPCSN NAITPGDCPPLELKNSVIQDGDMVD TGFGAMDFTALQDTKSNVPLDICNSI CKYPDYLKMVAEPYGDTLFFYLRREQ MFVRHFFNRSGTVGESVPTDLYIKGS GSTATLANSTYFPTPSGSMVTSDAQI FNKPYWMQRAQGHNNGICWGNQ LFVTVVDTTRSTNMSVCAAIANSDT TFKSSNFKEYLRHGEEFDLQFIFQLCK ITLSADIMTYIHSMNPAILEDWNFGL TTPPSGSLEDTYRFVTSQAITCQKTAP QKPKEDPFKDYVFWEVNLKEKFSAD LDQFPLGRKFLLQAGYRARPKFKAGK RSAPSASTTTPAKRKKTKK % Nucleotide Change 25.9% 27.0% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV31 L2 ATGCGGTCCAAACGCTCTACAAAACGCACTA AACGTGCGTCTGCTACACAATTATATCAAACA TGTAAAGCAGCAGGTACTTGTCCATCAGACG TTATACCTAAAATAGAACATACTACCATTGCA GACCAAATATTAAGGTATGGTAGTATGGGTG TTTTTTTTGGTGGGTTGGGTATTGGGTCCGGC TCTGGTACTGGGGGTCGCACTGGATATGTCC CTCTTAGTACACGTCCTTCTACAGTATCTGAG GCAAGTATACCTATTAGACCACCAGTTAGCAT TGACCCTGTAGGTCCCTTGGACCCCTCTATAG TAAGTCTTGTTGAAGAATCTGGAATTGTTGAT GTTGGTGCCCCTGCTCCTATACCACACCCTCC TACAACATCTGGGTTTGACATTGCTACAACTG CAGACACAACACCTGCAATTTTAGATGTAACA AGTGTTAGCACACATGAAAATCCTACTTTTAC TGATCCATCTGTATTGCAGCCTCCTACACCTG CAGAAACATCAGGTCATTTACTACTTTCATCA TCATCTATTAGCACACATAATTATGAGGAAAT ACCTATGGATACATTTATTGTTTCTACTAATA ATGAAAACATAACAAGTAGCACACCCATTCC AGGGGTGCGCCGTCCTGCACGTTTAGGGTTA TATAGTAAGGCTACACAACAAGTAAAAGTTA TTGATCCAACGTTTCTTAGTGCTCCAAAACAG CTAATTACATATGAAAACCCTGCCTATGAAAC TGTAAATGCTGAAGAATCTTTATACTTTTCCA ATACATCGCATAATATAGCCCCTGATCCCGAC TTTCTAGATATTATAGCATTACATAGGCCTGC CCTTACCTCACGTAGGAACACTGTTAGATATA GTAGACTAGGTAATAAACAAACTTTGCGCAC TCGTAGTGGTGCTACTATTGGTGCAAGGGTG CATTATTATTATGATATTAGTAGTATTAATCCT GCAGGTGAAAGTATTGAAATGCAACCTTTAG GGGCGTCTGCAACTACTACTTCTACTTTAAAT GATGGCTTATATGACATTTATGCAGACACTGA TTTTACTGTGGATACACCTGCCACACATAATG TTTCCCCTTCTACTGCTGTACAGTCCACATCTG CTGTGTCTGCCTATGTACCTACAAATACCACT GTGCCACTAAGTACAGGTTTTGACATTCCCAT ATTTTCTGGGCCTGATGTACCTATAGAGCATG CACCTACACAGGTTTTCCCATTTCCTTTGGCCC CTACAACGCCACAAGTGTCTATTTTTGTTGAT GGGGGTGATTTTTATTTGCACCCTAGTTATTA TATGTTAAAACGTCGACGTAAACGTGTATCAT ATTTTTTTACAGATGTCTCTGTGGCGGCCTAG ATGAGAAGCAAAAGAAGCACTAAAAGAACT AAAAGAGCCTCCGCAACCCAGCTGTATCAGA CCTGTAAAGCCGCAGGAACTTGTCCATCTGA CGTGATCCCTAAGATTGAGCACACCACAATC GCCGATCAGATTCTGCGCTATGGGAGCATGG GAGTGTTCTTTGGCGGGCTGGGCATTGGGAG TGGATCAGGCACAGGAGGCAGAACTGGCTA CGTGCCTCTGAGTACCAGGCCATCCACAGTCT CTGAAGCCAGTATCCCAATTAGACCCCCTGTG AGCATCGACCCCGTCGGACCTCTGGATCCAT CAATCGTGAGCCTGGTCGAGGAAAGCGGAA TTGTGGACGTCGGAGCACCAGCACCTATCCC ACACCCACCCACTACCTCCGGCTTCGACATTG CCACAACTGCTGATACCACACCCGCTATCCTG GACGTGACTAGCGTCTCCACCCATGAGAACC CCACCTTTACAGATCCTTCCGTGCTGCAGCCT CCAACACCCGCAGAAACTTCTGGGCACCTGC TGCTGAGCTCCTCTAGTATCAGTACCCATAAC TATGAGGAAATCCCTATGGACACCTTCATTGT GTCTACAAACAATGAGAATATCACTTCAAGC ACCCCCATTCCTGGGGTCCGGAGACCAGCTA GGCTGGGACTGTACTCCAAGGCAACACAGCA GGTGAAAGTCATTGATCCAACCTTTCTGTCTG CCCCCAAGCAGCTGATCACCTATGAGAACCC CGCATACGAAACAGTGAATGCCGAGGAAAG CCTGTATTTCTCCAACACCTCTCACAATATCGC CCCAGACCCCGATTTTCTGGATATCATTGCCC TGCATCGCCCTGCTCTGACTTCTAGGCGCAAC ACCGTGCGATACAGTCGGCTGGGCAACAAGC AGACACTGAGGACTCGCAGCGGCGCTACAAT TGGGGCACGAGTGCACTACTATTACGACATC TCCTCTATTAACCCAGCCGGAGAGTCCATCGA AATGCAGCCCCTGGGCGCTAGTGCAACTACC ACATCAACCCTGAATGACGGCCTGTATGATAT CTACGCTGACACCGATTTCACAGTGGATACTC CCGCCACCCATAACGTGTCTCCTAGTACAGCT GTCCAGTCAACTAGCGCAGTGAGCGCCTACG TCCCTACTAATACTACCGTGCCACTGTCAACC GGCTTCGACATCCCTATTTTTAGCGGGCCCGA TGTGCCTATTGAGCACGCACCAACACAGGTC TTCCCTTTTCCACTGGCCCCAACAACTCCCCA GGTGTCAATCTTCGTCGACGGGGGAGATTTT TATCTGCATCCCAGCTATTACATGCTGAAGCG ACGGAGAAAACGGGTGAGCTACTTCTTTACC GACGTGTCCGTCGCCGCT MRSKRSTKRTKRASATQLYQTCKAA GTCPSDVIPKIEHTTIADQILRYGSMG VFFGGLGIGSGSGTGGRTGYVPLSTR PSTVSEASIPIRPPVSIDPVGPLDPSIV SLVEESGIVDVGAPAPIPHPPTTSGF DIATTADTTPAILDVTSVSTHENPTFT DPSVLQPPTPAETSGHLLLSSSSISTH NYEEIPMDTFIVSTNNENITSSTPIPG VRRPARLGLYSKATQQVKVIDPTFLS APKQLITYENPAYETVNAEESLYFSNT SHNIAPDPDFLDIIALHRPALTSRRNT VRYSRLGNKQTLRTRSGATIGARVHY YYDISSINPAGESIEMQPLGASATTTS TLNDGLYDIYADTDFTVDTPATHNVS PSTAVQSTSAVSAYVPTNTTVPLSTG FDIPIFSGPDVPIEHAPTQVFPFPLAP TTPQVSIFVDGGDFYLHPSYYMLKRR RKRVSYFFTDVSVAA Page 20 of 48 % Nucleotide Change 28.3% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV33 E1 ATGGCCGATCCTGAAGGTACAAATGGGGCTG GGATGGGGTGTACTGGTTGGTTTGAGGTAG AAGCAGTCATAGAGAGAAGAACAGGAGATA ATATTTCAGAAGATGAGGATGAAACAGCAGA TGACAGTGGCACGGATTTACTAGAGTTTATA GATGATTCTATGGAAAATAGTATACAGGCAG ACACAGAGGCAGCCCGGGCATTGTTTAATAT ACAGGAAGGGGAGGATGATTTAAATGCTGT GTGTGCACTAAAACGAAAGTTTGCCGCATGT TCACAAAGTGCTGCGGAGGACGTTGTTGATC GTGCTGCAAACCCGTGTAGAACGTCTATTAAT AAAAATAAAGAATGCACATACAGAAAACGAA AAATAGATGAGCTAGAAGACAGCGGATATG GCAATACTGAAGTGGAAACTCAGCAGATGGT ACAACAGGTAGAAAGTCAAAATGGCGACAC AAACTTAAATGACTTAGAATCTAGTGGGGTG GGGGATGATTCAGAAGTAAGCTGTGAGACA AATGTAGATAGCTGTGAAAATGTTACGTTGC AGGAAATTAGTAATGTTCTACATAGTAGTAAT ACAAAAGCAAATATATTATATAAATTTAAAGA GGCCTATGGAATAAGTTTTATGGAATTAGTA AGACCATTTAAAAGTGATAAAACAAGCTGTA CAGATTGGTGTATAACAGGATATGGAATTAG TCCATCAGTAGCAGAAAGTTTAAAAGTATTA ATTAAACAGCATAGTTTGTATACTCATTTACA ATGTTTAACTTGCGATAGAGGAATAATAATAT TATTGTTAATTAGATTTAGGTGTAGCAAAAAC AGGTTAACAGTAGCAAAACTAATGAGTAATT TATTATCAATACCTGAAACATGTATGGTTATA GAGCCACCAAAATTACGGAGCCAAACATGTG CATTGTATTGGTTTAGAACAGCAATGTCAAAC ATTAGTGATGTACAAGGTACAACACCTGAAT GGATAGATAGACTAACTGTTTTACAACATAG CTTTAATGATAATATATTTGATTTAAGTGAAA TGGTACAGTGGGCATATGATAACGAGTTAAC GGACGATAGTGACATTGCATATTATTATGCAC AACTTGCAGATTCAAATAGTAATGCTGCTGCA TTTTTAAAAAGTAACTCACAAGCAAAAATAGT AAAGGACTGTGGAATAATGTGTAGACATTAT AAAAAAGCAGAAAAACGTAAAATGTCAATAG GACAATGGATACAAAGTAGATGTGAAAAAAC AAATGATGGAGGAAATTGGAGACCAATAGT ACAGTTGTTAAGATATCAAAACATTGAATTTA CAGCATTTTTAGGTGCATTTAAAAAGTTTTTA AAAGGTATACCAAAAAAAAGCTGTATGCTAA TTTGTGGACCAGCAAATACAGGAAAGTCATA TTTTGGAATGAGTTTAATACAGTTTTTAAAAG GGTGTGTTATATCATGTGTAAATTCTAAAAGT CACTTTTGGTTGCAGCCATTATCAGATGCAAA AATAGGAATGATAGATGATGTAACGCCAATA AGTTGGACATATATAGATGATTACATGAGAA ATGCGTTAGATGGAAATGAAATTTCAATAGA TGTGAAACATAGGGCATTAGTGCAATTAAAA TGTCCACCACTGCTTCTTACCTCAAATACAAA TGCAGGCACAGACTCTAGATGGCCATATTTA CATAGTAGATTAACAGTATTTGAATTTAAAAA TCCATTCCCATTTGATGAAAATGGTAACCCAG TGTATGCAATAAATGATGAAAATTGGAAATC CTTTTTCTCAAGGACGTGGTGCAAATTAGATT TAATAGAGGAAGAGGACAAGGAAAACCATG GAGGAAATATCAGCACGTTTAAATGCAGTGC AGGAGAAAATACTAGATCTTTACGAAGCTGA ATGGCTGACCCTGAAGGCACTAACGGGGCTG GGATGGGCTGTACTGGCTGGTTTGAGGTGG AGGCTGTGATTGAAAGACGGACTGGGGACA ACATCAGCGAAGACGAGGATGAAACAGCCG ACGATTCCGGGACTGATCTGCTGGAGTTCAT TGACGATTCTATGGAAAATAGTATCCAGGCA GACACCGAGGCAGCTCGAGCACTGTTCAACA TCCAGGAGGGAGAAGACGATCTGAATGCAG TGTGCGCCCTGAAGAGAAAATTTGCAGCCTG TTCACAGAGCGCTGCAGAGGACGTGGTCGAT AGAGCCGCTAACCCTTGCAGGACATCCATTA ACAAGAACAAGGAATGTACTTATCGGAAGAG AAAAATCGACGAGCTGGAAGATAGTGGGTA CGGAAACACCGAGGTGGAAACACAGCAGAT GGTGCAGCAGGTCGAGAGCCAGAATGGCGA CACAAACCTGAATGATCTGGAAAGCTCCGGC GTGGGGGACGATTCAGAGGTCAGCTGCGAA ACCAACGTGGATTCTTGTGAGAATGTCACACT GCAGGAAATCAGTAATGTGCTGCACTCTAGT AACACCAAGGCCAACATCCTGTACAAGTTTA AAGAGGCTTACGGCATCAGCTTCATGGAACT GGTGCGGCCTTTTAAGTCAGACAAAACTAGC TGCACCGATTGGTGTATTACAGGATATGGCA TCTCCCCATCTGTGGCCGAGTCCCTGAAGGTC CTGATCAAGCAGCACTCTCTGTACACCCATCT GCAGTGCCTGACATGTGACCGCGGGATCATT ATCCTGCTGCTGATCAGGTTCCGCTGCAGCA AGAACCGACTGACCGTGGCCAAACTGATGTC CAATCTGCTGTCTATTCCAGAGACATGCATGG TCATCGAACCCCCTAAGCTGCGATCCCAGACT TGTGCTCTGTATTGGTTTCGGACCGCAATGTC CAACATTTCTGACGTGCAGGGCACCACACCC GAGTGGATCGATAGGCTGACAGTCCTGCAGC ACAGTTTCAACGACAATATTTTTGATCTGTCA GAGATGGTGCAGTGGGCATACGACAACGAA CTGACTGACGATTCTGATATCGCCTACTATTA CGCTCAGCTGGCAGATAGTAACTCAAATGCA GCCGCTTTCCTGAAAAGCAATTCCCAGGCCA AGATTGTGAAAGACTGCGGCATCATGTGTAG GCATTATAAGAAAGCTGAGAAGCGCAAAATG TCTATTGGGCAGTGGATCCAGAGTCGCTGCG AAAAGACTAACGACGGCGGGAATTGGCGCC CCATTGTGCAGCTGCTGCGATACCAGAACAT CGAGTTCACCGCCTTTCTGGGGGCTTTCAAG AAATTTCTGAAAGGAATTCCCAAGAAAAGCT GCATGCTGATCTGTGGGCCTGCTAACACCGG AAAGAGTTACTTCGGCATGTCACTGATTCAGT TTCTGAAAGGATGCGTGATCTCATGTGTCAAT TCTAAGAGTCACTTTTGGCTGCAGCCACTGTC CGATGCCAAGATTGGCATGATCGACGATGTG ACTCCCATTTCTTGGACCTATATCGACGATTA CATGAGAAACGCTCTGGACGGGAATGAGATT TCCATCGATGTGAAGCACAGGGCACTGGTCC AGCTGAAATGTCCACCCCTGCTGCTGACATCT AACACTAATGCAGGAACAGACTCACGGTGGC CCTATCTGCATAGCAGACTGACTGTGTTCGA GTTTAAGAACCCTTTCCCATTTGACGAAAACG GCAATCCTGTCTACGCCATCAACGATGAGAA TTGGAAGAGTTTCTTTTCAAGAACCTGGTGCA AACTGGACCTGATTGAGGAAGAGGATAAGG AGAACCATGGAGGCAATATCAGCACTTTCAA GTGTTCCGCCGGCGAAAATACCCGAAGCCTG CGGTCC MADPEGTNGAGMGCTGWFEVEAV IERRTGDNISEDEDETADDSGTDLLE FIDDSMENSIQADTEAARALFNIQEG EDDLNAVCALKRKFAACSQSAAEDV VDRAANPCRTSINKNKECTYRKRKID ELEDSGYGNTEVETQQMVQQVESQ NGDTNLNDLESSGVGDDSEVSCETN VDSCENVTLQEISNVLHSSNTKANILY KFKEAYGISFMELVRPFKSDKTSCTD WCITGYGISPSVAESLKVLIKQHSLYT HLQCLTCDRGIIILLLIRFRCSKNRLTV AKLMSNLLSIPETCMVIEPPKLRSQT CALYWFRTAMSNISDVQGTTPEWID RLTVLQHSFNDNIFDLSEMVQWAY DNELTDDSDIAYYYAQLADSNSNAA AFLKSNSQAKIVKDCGIMCRHYKKAE KRKMSIGQWIQSRCEKTNDGGNW RPIVQLLRYQNIEFTAFLGAFKKFLKGI PKKSCMLICGPANTGKSYFGMSLIQF LKGCVISCVNSKSHFWLQPLSDAKIG MIDDVTPISWTYIDDYMRNALDGNE ISIDVKHRALVQLKCPPLLLTSNTNAG TDSRWPYLHSRLTVFEFKNPFPFDEN GNPVYAINDENWKSFFSRTWCKLDL IEEEDKENHGGNISTFKCSAGENTRS LRS Page 21 of 48 % Nucleotide Change 27.0% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV33 E2 ATGGAGGAAATATCAGCACGTTTAAATGCAG TGCAGGAGAAAATACTAGATCTTTACGAAGC TGATAAAACTGATTTACCATCACAAATTGAAC ATTGGAAACTGATACGCATGGAGTGTGCTTT ATTGTATACAGCCAAACAAATGGGATTTTCAC ATTTATGCCACCAGGTGGTGCCTTCTTTGTTA GCATCAAAGACCAAAGCATTTCAAGTAATTG AACTACAAATGGCATTAGAGACATTAAGTAA ATCACAGTATAGTACAAGCCAATGGACATTG CAACAAACAAGCTTAGAGGTGTGGCTTTGTG AACCACCAAAATGTTTTAAAAAACAAGGAGA AACAGTAACTGTGCAATATGACAATGACAAA AAAAATACAATGGATTATACAAACTGGGGTG AAATATATATTATAGAGGAAGATACATGTAC TATGGTTACAGGGAAAGTAGATTATATAGGT ATGTATTATATACATAACTGTGAAAAGGTATA TTTTAAATATTTTAAAGAGGATGCTGCAAAGT ATTCTAAAACACAAATGTGGGAAGTACATGT GGGTGGTCAGGTAATTGTTTGTCCTACGTCTA TATCTAGCAACCAAATATCCACTACTGAAACT GCTGACATACAGACAGACAACGATAACCGAC CACCACAAGCAGCGGCCAAACGACGACGACC TGCAGACACCACAGACACCGCCCAGCCCCTT ACAAAGCTGTTCTGTGCAGACCCCGCCTTGG ACAATAGAACAGCACGTACTGCAACTAACTG CACAAACAAGCAGCGGACTGTGTGTAGTTCT AACGTTGCACCTATAGTGCATTTAAAAGGTG AATCAAATAGTTTAAAATGTTTAAGATACAGA TTAAAACCTTATAAAGAGTTGTATAGTTCTAT GTCATCCACCTGGCATTGGACCAGTGACAAC AAAAATAGTAAAAATGGAATTGTAACTGTAA CATTTGTAACTGAACAGCAACAACAAATGTTT TTAGGTACCGTAAAAATACCACCTACTGTGCA AATAAGTACTGGATTTATGACATTATAA MEEISARLNAVQEKILDLYEADKTDL PSQIEHWKLIRMECALLYTAKQMGF SHLCHQVVPSLLASKTKAFQVIELQM ALETLSKSQYSTSQWTLQQTSLEVW LCEPPKCFKKQGETVTVQYDNDKKN TMDYTNWGEIYIIEEDTCTMVTGKV DYIGMYYIHNCEKVYFKYFKEDAAKY SKTQMWEVHVGGQVIVCPTSISSN QISTTETADIQTDNDNRPPQAAAKR RRPADTTDTAQPLTKLFCADPALDN RTARTATNCTNKQRTVCSSNVAPIV HLKGESNSLKCLRYRLKPYKELYSSMS STWHWTSDNKNSKNGIVTVTFVTE QQQQMFLGTVKIPPTVQISTGFMTL HPV33 E4 TTGTTTGTCCTACGTCTATATCTAGCAACCAA ATATCCACTACTGAAACTGCTGACATACAGAC AGACAACGATAACCGACCACCACAAGCAGCG GCCAAACGACGACGACCTGCAGACACCACAG ACACCGCCCAGCCCCTTACAAAGCTGTTCTGT GCAGACCCCGCCTTGGACAATAGAACAGCAC GTACTGCAACTAACTGCACAAACAAGCAGCG GACTGTGTGTAGTTCTAACGTTGCACCTATAG ATGATATTTGTTTTTGTATTATGTTTTATATTG TTTTTATGCTTATCCTTATTATTACGTCCTTTA ATACTTTCCATTTCTACCTATGCTTGGTTGCTG GTGTTGGTATTGCTGCTTTGGGTGTTTGTGG GATCTCCTTTAAAAATTTTTTTTTGCTATTTGT TGTTTTTATATTTACCAATGATGTGTATTAATT TTCATGCACAGCATATGACACAACAAGAGTA A ATGTTTCAAGACACTGAGGAAAAACCACGAA CATTGCATGATTTGTGCCAAGCATTGGAGAC AACTATACACAACATTGAACTACAGTGCGTG GAATGCAAAAAACCTTTGCAACGATCTGAGG TATATGATTTTGCATTTGCAGATTTAACAGTT GTATATAGAGAGGGAAATCCATTTGGAATAT GTAAACTGTGTTTGCGGTTCTTATCTAAAATT AGTGAATATAGACATTATAATTATTCTGTATA TGGAAATACATTAGAACAAACAGTTAAAAAA CCTTTAAATGAAATATTAATTAGGTGTATTAT ATGTCAAAGACCTTTGTGTCCTCAAGAAAAA AAACGACATGTGGATTTAAACAAACGATTTC ATAATATTTCGGGTCGTTGGGCAGGGCGCTG TGCGGCGTGTTGGAGGTCCCGACGTAGAGA AACTGCACTGTGA ATGAGAGGACACAAGCCAACGTTAAAGGAA TATGTTTTAGATTTATATCCTGAACCAACTGA CCTATACTGCTATGAGCAATTAAGTGACAGCT CAGATGAGGATGAAGGCTTGGACCGGCCAG ATGGACAAGCACAACCAGCCACAGCTGATTA CTACATTGTAACCTGTTGTCACACTTGTAACA CCACAGTTCGTTTATGTGTCAACAGTACAGCA ATGGAGGAGATTAGCGCAAGACTGAACGCC GTGCAGGAAAAGATTCTGGACCTGTATGAAG CCGACAAGACCGACCTGCCAAGCCAGATCGA GCACTGGAAGCTGATTCGAATGGAATGCGCC CTGCTGTACACCGCTAAACAGATGGGGTTCA GCCACCTGTGTCATCAGGTGGTCCCATCACTG CTGGCAAGCAAGACTAAAGCCTTTCAGGTCA TCGAGCTGCAGATGGCCCTGGAAACCCTGAG TAAGTCACAGTACAGCACCTCCCAGTGGACA CTGCAGCAGACTTCCCTGGAAGTGTGGCTGT GCGAACCCCCTAAGTGTTTCAAGAAACAGGG CGAGACAGTGACTGTCCAGTATGACAACGAT AAGAAAAATACCATGGACTACACAAACTGGG GGGAAATCTATATCATTGAGGAAGACACCTG CACAATGGTGACCGGAAAGGTCGATTACATC GGCATGTACTATATTCACAACTGTGAGAAGG TGTACTTCAAGTACTTCAAGGAAGACGCCGC TAAGTATTCTAAAACACAGATGTGGGAGGTG CATGTCGGCGGGCAGGTCATCGTCTGCCCCA CTAGTATCAGCTCCAACCAGATTAGCACCACA GAAACCGCTGATATTCAGACAGACAACGATA ATAGGCCACCACAGGCAGCAGCTAAGCGAA GAAGGCCAGCTGACACTACCGATACTGCACA GCCTCTGACCAAACTGTTCTGCGCAGACCCA GCCCTGGATAATCGGACAGCTAGAACTGCAA CCAACTGCACAAATAAGCAGCGCACTGTGTG TTCTAGTAACGTGGCCCCCATCGTCCACCTGA AGGGAGAGTCTAATAGTCTGAAATGTCTGCG CTATCGACTGAAGCCTTACAAAGAACTGTATT CAAGCATGTCCTCTACTTGGCATTGGACCTCC GATAACAAGAATTCTAAAAACGGCATTGTGA CAGTCACTTTCGTGACCGAGCAGCAGCAGCA GATGTTTCTGGGGACCGTGAAGATCCCTCCA ACAGTCCAGATTAGCACAGGCTTCATGACTCT G ATGTTCGTGCTGAGGCTGTACCTGGCAACTA AGTATCCCCTGCTGAAACTGCTGACCTACCGA CAGACCACCATCACTGACCATCACAAGCAGC GGCCCAACGACGATGACCTGCAGACCCCTCA GACACCCCCTTCCCCACTGCAGTCTTGCAGTG TGCAGACACCACCCTGGACTATCGAGCAGCA CGTCCTGCAGCTGACTGCCCAGACCAGCTCC GGCCTGTGTGTGGTCCTGACCCTGCATCTG ATGATTTTTGTGTTTGTCCTGTGTTTTATCCTG TTTCTGTGCCTGAGCCTGCTGCTGAGACCACT GATTCTGTCCATTTCTACTTATGCCTGGCTGCT GGTGCTGGTCCTGCTGCTGTGGGTGTTCGTC GGCAGCCCCCTGAAGATCTTCTTTTGCTACCT GCTGTTCCTGTATCTGCCTATGATGTGTATTA ACTTTCACGCTCAGCATATGACCCAGCAGGA G ATGTTTCAGGACACCGAGGAGAAGCCAAGA ACTCTGCATGATCTGTGCCAGGCTCTGGAGA CCACCATTCACAATATCGAACTGCAGTGCGTG GAGTGTAAGAAACCACTGCAGCGCAGCGAA GTCTACGACTTCGCATTTGCCGATCTGACTGT GGTCTATCGGGAGGGCAACCCCTTCGGGATC TGCAAGCTGTGTCTGCGATTTCTGAGCAAAA TTTCCGAATACAGGCACTACAACTATTCTGTG TATGGGAATACCCTGGAGCAGACAGTCAAGA AACCCCTGAATGAAATCCTGATTCGGTGCATC ATTTGTCAGAGACCCCTGTGCCCTCAGGAGA AGAAAAGGCACGTGGACCTGAACAAGCGCTT CCATAATATCTCTGGACGATGGGCTGGACGA TGCGCAGCTTGTTGGAGAAGTCGGAGAAGG GAAACCGCCCTG ATGCGAGGCCACAAGCCCACCCTGAAAGAGT ATGTCCTGGACCTGTATCCCGAGCCCACCGA CCTGTATTGTTATGAGCAGCTGTCAGACAGCT CCGACGAGGATGAAGGACTGGACAGGCCAG ATGGACAGGCTCAGCCTGCAACCGCTGATTA CTATATCGTGACTTGCTGTCACACCTGCAACA CCACAGTGCGGCTGTGTGTCAATTCTACAGC HPV33 E5 HPV33 E6 HPV33 E7 Page 22 of 48 % Nucleotide Change 26.8% LFVLRLYLATKYPLLKLLTYRQTTITDH HKQRPNDDDLQTPQTPPSPLQSCSV QTPPWTIEQHVLQLTAQTSSGLCVV LTLHL 27.2% MIFVFVLCFILFLCLSLLLRPLILSISTYA WLLVLVLLLWVFVGSPLKIFFCYLLFL YLPMMCINFHAQHMTQQE 26.1% MFQDTEEKPRTLHDLCQALETTIHNI ELQCVECKKPLQRSEVYDFAFADLTV VYREGNPFGICKLCLRFLSKISEYRHY NYSVYGNTLEQTVKKPLNEILIRCIICQ RPLCPQEKKRHVDLNKRFHNISGRW AGRCAACWRSRRRETAL 26.9% MRGHKPTLKEYVLDLYPEPTDLYCYE QLSDSSDEDEGLDRPDGQAQPATA DYYIVTCCHTCNTTVRLCVNSTASDL RTIQQLLMGTVNIVCPTCAQQ 24.0% Gene HPV33 L1 PaVE DNA Sequence Optimized DNA Sequence AGTGACCTACGAACCATACAGCAACTACTTAT GGGCACAGTGAATATTGTGTGCCCTACCTGT GCACAACAATAA AAGCGACCTGAGAACTATCCAGCAGCTGCTG ATGGGCACCGTGAACATTGTCTGCCCCACAT GTGCCCAGCAG ATGTCCGTGTGGCGGCCTAGTGAGGCCACAG TGTACCTGCCTCCTGTACCTGTATCTAAAGTT GTCAGCACTGATGAATATGTGTCTCGCACAA GCATTTATTATTATGCTGGTAGTTCCAGACTT CTTGCTGTTGGCCATCCATATTTTTCTATTAAA AATCCTACTAACGCTAAAAAATTATTGGTACC CAAAGTATCAGGCTTGCAATATAGGGTTTTTA GGGTCCGTTTACCAGATCCTAATAAATTTGGA TTTCCTGACACCTCCTTTTATAACCCTGATACA CAACGATTAGTATGGGCATGTGTAGGCCTTG AAATAGGTAGAGGGCAGCCATTAGGCGTTG GCATAAGTGGTCATCCTTTATTAAACAAATTT GATGACACTGAAACCGGTAACAAGTATCCTG GACAACCGGGTGCTGATAATAGGGAATGTTT ATCCATGGATTATAAACAAACACAGTTATGTT TACTTGGATGTAAGCCTCCAACAGGGGAACA TTGGGGTAAAGGTGTTGCTTGTACTAATGCA GCACCTGCCAATGATTGTCCACCTTTAGAACT TATAAATACTATTATTGAGGATGGTGATATG GTGGACACAGGATTTGGTTGCATGGATTTTA AAACATTGCAGGCTAATAAAAGTGATGTTCC TATTGATATTTGTGGCAGTACATGCAAATATC CAGATTATTTAAAAATGACTAGTGAGCCTTAT GGTGATAGTTTATTTTTCTTTCTTCGACGTGA ACAAATGTTTGTAAGACACTTTTTTAATAGGG CTGGTACATTAGGAGAGGCTGTTCCCGATGA CCTGTACATTAAAGGTTCAGGAACTACTGCCT CTATTCAAAGCAGTGCTTTTTTTCCCACTCCTA GTGGATCAATGGTTACTTCCGAATCTCAGTTA TTTAATAAGCCATATTGGCTACAACGTGCACA AGGTCATAATAATGGTATTTGTTGGGGCAAT CAGGTATTTGTTACTGTGGTAGATACCACTCG CAGTACTAATATGACTTTATGCACACAAGTAA CTAGTGACAGTACATATAAAAATGAAAATTTT AAAGAATATATAAGACATGTTGAAGAATATG ATCTACAGTTTGTTTTTCAACTATGCAAAGTT ACCTTAACTGCAGAAGTTATGACATATATTCA TGCTATGAATCCAGATATTTTAGAAGATTGGC AATTTGGTTTAACACCTCCTCCATCTGCTAGTT TACAGGATACCTATAGGTTTGTTACCTCTCAG GCTATTACGTGTCAAAAAACAGTACCTCCAAA GGAAAAGGAAGACCCCTTAGGTAAATATACA TTTTGGGAAGTGGATTTAAAGGAAAAATTTT CAGCAGATTTAGATCAGTTTCCTTTGGGACGC AAGTTTTTATTACAGGCAGGTCTTAAAGCAAA ACCTAAACTTAAACGTGCAGCCCCCACATCCA CCCGCACATCGTCTGCAAAACGCAAAAAGGT TAAAAAATAA ATGTCAGTGTGGAGACCCAGCGAGGCTACCG TGTATCTGCCCCCAGTCCCCGTGAGCAAAGT GGTGTCAACCGATGAGTATGTGAGCCGCACC TCCATCTACTATTACGCTGGAAGCTCCCGACT GCTGGCAGTGGGACACCCCTATTTTAGCATT AAGAACCCTACAAATGCCAAGAAACTGCTGG TGCCTAAAGTCTCCGGGCTGCAGTATAGGGT GTTTAGGGTCCGCCTGCCCGACCCTAACAAG TTTGGATTCCCAGACACATCTTTCTACAATCC CGATACTCAGCGACTGGTGTGGGCATGCGTC GGACTGGAGATCGGAAGAGGACAGCCACTG GGAGTGGGCATTAGTGGACACCCTCTGCTGA ACAAGTTCGACGATACAGAGACTGGCAACAA GTATCCTGGGCAGCCAGGAGCTGACAACCGC GAATGTCTGAGCATGGATTACAAGCAGACCC AGCTGTGCCTGCTGGGCTGTAAGCCCCCTAC AGGCGAGCATTGGGGGAAAGGAGTGGCCTG CACTAACGCCGCTCCAGCTAATGACTGTCCAC CCCTGGAGCTGATCAACACCATCATTGAAGA CGGCGATATGGTCGACACTGGCTTTGGGTGC ATGGATTTCAAGACCCTGCAGGCCAACAAGA GTGACGTGCCCATCGATATTTGCGGCTCAAC CTGTAAGTATCCAGACTACCTGAAAATGACTT CCGAGCCCTATGGGGATTCTCTGTTCTTTTTC CTGCGGAGAGAACAGATGTTTGTCCGACACT TTTTCAACCGAGCAGGAACCCTGGGAGAGGC TGTGCCCGACGATCTGTACATCAAGGGATCA GGCACCACAGCAAGCATTCAGTCTAGTGCCT TTTTCCCAACCCCCTCCGGCTCTATGGTGACA AGTGAATCACAGCTGTTTAATAAGCCTTACTG GCTGCAGCGAGCCCAGGGACATAACAATGG CATCTGCTGGGGGAACCAGGTGTTCGTCACT GTGGTCGACACTACCCGCTCTACTAATATGAC CCTGTGTACACAGGTCACTAGCGATTCCACAT ACAAGAACGAGAACTTCAAGGAATACATTCG GCACGTGGAGGAATACGACCTGCAGTTTGTG TTCCAGCTGTGCAAGGTCACCCTGACAGCAG AAGTGATGACCTACATCCATGCCATGAATCCC GACATTCTGGAAGATTGGCAGTTTGGACTGA CACCTCCACCCTCTGCTAGTCTGCAGGATACT TATAGATTCGTCACCAGCCAGGCAATCACCTG TCAGAAGACAGTGCCTCCAAAGGAGAAAGA AGACCCTCTGGGCAAATACACCTTTTGGGAG GTGGATCTGAAGGAAAAATTCAGCGCCGACC TGGATCAGTTTCCACTGGGCAGGAAGTTCCT GCTGCAGGCTGGGCTGAAGGCAAAACCTAA GCTGAAACGCGCAGCCCCAACTTCCACCAGA ACATCAAGCGCTAAAAGGAAGAAAGTGAAG AAA Page 23 of 48 Protein Sequence MSVWRPSEATVYLPPVPVSKVVSTD EYVSRTSIYYYAGSSRLLAVGHPYFSIK NPTNAKKLLVPKVSGLQYRVFRVRLP DPNKFGFPDTSFYNPDTQRLVWAC VGLEIGRGQPLGVGISGHPLLNKFDD TETGNKYPGQPGADNRECLSMDYK QTQLCLLGCKPPTGEHWGKGVACT NAAPANDCPPLELINTIIEDGDMVDT GFGCMDFKTLQANKSDVPIDICGST CKYPDYLKMTSEPYGDSLFFFLRREQ MFVRHFFNRAGTLGEAVPDDLYIKG SGTTASIQSSAFFPTPSGSMVTSESQ LFNKPYWLQRAQGHNNGICWGNQ VFVTVVDTTRSTNMTLCTQVTSDST YKNENFKEYIRHVEEYDLQFVFQLCK VTLTAEVMTYIHAMNPDILEDWQF GLTPPPSASLQDTYRFVTSQAITCQK TVPPKEKEDPLGKYTFWEVDLKEKFS ADLDQFPLGRKFLLQAGLKAKPKLKR AAPTSTRTSSAKRKKVKK % Nucleotide Change 26.4% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV33 L2 ATGAGACACAAACGATCTACAAGGCGCAAGC GTGCATCTGCAACACAACTATACCAAACATGC AAGGCCACAGGCACCTGCCCACCCGATGTTA TTCCTAAAGTGGAAGGAAGTACCATAGCAGA TCAAATTCTTAAATATGGCAGTTTAGGGGTTT TTTTTGGTGGTTTAGGTATTGGCACAGGCTCT GGTTCAGGTGGAAGGACTGGCTATGTACCTA TTGGTACTGACCCACCTACAGCTGCAATCCCC TTGCAGCCTATACGTCCTCCGGTTACTGTAGA CACTGTTGGACCTTTAGACTCGTCTATAGTGT CATTAATAGAAGAAACAAGTTTTATAGAGGC AGGTGCACCAGCCCCATCTATTCCTACACCAT CAGGTTTTGATGTTACTACATCTGCAGATACT ACACCTGCAATTATTAATGTTTCATCTGTTGG GGAGTCATCTATTCAAACTATTTCTACACATT TAAATCCCACATTTACTGAACCATCTGTACTA CACCCTCCAGCGCCTGCAGAAGCCTCTGGAC ATTTTATATTTTCTTCCCCTACTGTTAGCACAC AAAGTTATGAAAACATACCAATGGATACCTTT GTTGTTTCCACAGACAGTAGTAATGTAACATC AAGCACGCCCATTCCAGGGTCTCGCCCTGTG GCACGCCTTGGTTTATATAGTCGCAATACCCA ACAGGTTAAGGTTGTTGACCCTGCTTTTTTAA CATCGCCTCATAAACTTATAACATATGATAAT CCTGCATTTGAAAGCTTTGACCCTGAAGACAC ATTACAATTTCAACATAGTGATATATCACCTG CTCCTGATCCTGACTTTCTAGATATTATTGCAT TACATAGGCCTGCTATTACATCTCGTAGACAT ACTGTGCGTTTTAGTAGAGTAGGTCAAAAAG CCACACTTAAAACTCGCAGTGGTAAACAAATT GGAGCTAGAATACATTATTATCAGGATTTAA GTCCTATTGTGCCTTTAGACCACACCGTGCCA AATGAACAATATGAATTACAGCCTTTACATGA TACTTCTACATCGTCTTATAGTATTAATGATG GTTTGTATGATGTTTATGCTGACGATGTGGAT AATGTACACACCCCAATGCAACACTCATACAG TACGTTTGCAACAACACGTACCAGCAATGTGT CTATACCTTTAAATACAGGATTTGATACTCCT GTTATGTCTGGCCCTGATATACCTTCCCCTTTA TTTCCCACATCTAGCCCATTTGTTCCTATTTCG CCTTTTTTTCCTTTTGACACCATTGTTGTAGAC GGTGCTGACTTTGTTTTACATCCTAGTTATTTT ATTTTACGTCGCAGGCGTAAACGTTTTCCATA TTTTTTTACAGATGTCCGTGTGGCGGCCTAG ATGCGACACAAGAGAAGCACCAGAAGGAAG AGAGCAAGCGCCACCCAGCTGTATCAGACCT GTAAAGCCACCGGGACCTGCCCTCCAGACGT GATCCCCAAGGTCGAGGGCAGTACCATCGCC GATCAGATTCTGAAATACGGATCACTGGGCG TGTTCTTTGGAGGACTGGGAATCGGAACTGG ATCCGGATCTGGAGGACGAACCGGATATGTG CCAATTGGGACTGACCCACCTACCGCAGCTAT CCCACTGCAGCCCATTCGCCCACCCGTGACAG TCGACACTGTGGGCCCCCTGGATAGCTCCAT CGTCAGCCTGATTGAGGAAACATCCTTCATCG AGGCTGGAGCACCAGCACCTTCCATTCCAACT CCCTCTGGGTTTGACGTGACCACATCCGCTGA TACTACCCCTGCAATCATTAACGTGTCTAGTG TCGGAGAATCAAGCATCCAGACCATTAGCAC ACATCTGAATCCTACCTTCACAGAGCCATCCG TGCTGCACCCTCCAGCTCCAGCAGAAGCCTCT GGCCATTTCATCTTTTCCTCTCCAACTGTGAG CACCCAGTCCTACGAGAACATTCCCATGGAC ACCTTTGTGGTCAGCACAGATAGTTCAAATGT GACAAGCTCCACTCCTATCCCAGGATCCCGAC CAGTCGCACGACTGGGACTGTACTCTAGAAA CACTCAGCAGGTGAAGGTGGTCGACCCAGCT TTCCTGACCAGTCCCCATAAACTGATCACATA TGATAATCCTGCATTCGAGTCTTTTGACCCAG AAGATACACTGCAGTTCCAGCACTCTGACATC AGTCCCGCTCCTGACCCAGATTTTCTGGATAT CATTGCCCTGCACAGGCCTGCTATTACTTCAC GGAGACATACCGTGAGATTCAGCAGGGTCG GGCAGAAGGCAACCCTGAAAACACGGTCCG GGAAGCAGATCGGAGCCAGAATTCACTACTA TCAGGACCTGAGCCCTATCGTGCCACTGGAT CATACCGTCCCCAACGAGCAGTACGAACTGC AGCCTCTGCACGACACTAGTACCTCTAGTTAT TCAATTAACGACGGCCTGTACGATGTGTATG CCGACGATGTGGATAATGTCCACACCCCCAT GCAGCATAGTTATTCAACATTCGCTACAACTC GGACTTCAAACGTGAGCATCCCTCTGAATAC AGGGTTTGACACTCCCGTGATGTCTGGACCT GATATTCCCAGTCCTCTGTTCCCCACCTCAAG CCCCTTTGTGCCTATCTCCCCATTCTTTCCCTT CGACACAATTGTGGTCGACGGCGCCGATTTC GTGCTGCACCCTAGCTACTTTATCCTGAGGCG CCGACGGAAAAGGTTTCCATATTTCTTTACCG ATGTGCGCGTCGCAGCC MRHKRSTRRKRASATQLYQTCKATG TCPPDVIPKVEGSTIADQILKYGSLGV FFGGLGIGTGSGSGGRTGYVPIGTDP PTAAIPLQPIRPPVTVDTVGPLDSSIV SLIEETSFIEAGAPAPSIPTPSGFDVTT SADTTPAIINVSSVGESSIQTISTHLNP TFTEPSVLHPPAPAEASGHFIFSSPTV STQSYENIPMDTFVVSTDSSNVTSST PIPGSRPVARLGLYSRNTQQVKVVD PAFLTSPHKLITYDNPAFESFDPEDTL QFQHSDISPAPDPDFLDIIALHRPAIT SRRHTVRFSRVGQKATLKTRSGKQIG ARIHYYQDLSPIVPLDHTVPNEQYEL QPLHDTSTSSYSINDGLYDVYADDVD NVHTPMQHSYSTFATTRTSNVSIPL NTGFDTPVMSGPDIPSPLFPTSSPFV PISPFFPFDTIVVDGADFVLHPSYFILR RRRKRFPYFFTDVRVAA Page 24 of 48 % Nucleotide Change 28.4% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV35 E1 ATGGCTGATCCTGCAGGTACAGATGAAGGG GAGGGGACGGGATGTAATGGATGGTTTTTTG TAGAAGCAGTAGTTAGTAGACGTACGGGGG ATCCAGTGTCAGAGGACGAAAATGAAGATG ACTGTGACAGGGGGGAGGATATGGTGGACT TTATAAATGATACAGATATATTAAACATACAG GCAGAAACAGAGACAGCACAAGCATTATTTC ATGCACAGGAGGAGCAAACACACAAAGAGG CTGTACAGGTCCTAAAACGAAAGTATGCTAG TAGTCCACTTAGCAGCGTGAGCTTATGTGTTA ATAATAACATAAGTCCACGTTTAAAAGCTATT TGCATTGAAAATAAAAATACAGCAGCAAAGC GACGATTATTTGAACTACCAGACAGCGGTTA TGGCAATTCTGAAGTGGAAATACAGCAGATA CAACAGGTAGAGGGGCATGATACAGTTGAA CAATGTAGTATGGGCAGTGGGGATAGTATAA CCTCTAGTAGCGATGAAAGACATGATGAGAC TCCAACGCGAGACATAATACAAATACTAAAA TGTAGTAATGCAAACGCAGCTATGTTGGCTA AATTTAAAGAACTATTTGGTATTAGTTTTACA GAACTTATTAGACCATTTAAGAGTGATAAATC CACATGTACAGATTGGTGTGTGGCCGCATTT GGAATAGCCCCAAGTGTGGCGGAAAGTTTAA AAACATTAATTAAACCATATTGTTTATATATA CATATACAATGTTTATCGTGTTCATGGGGTAT GGTAATTCTAGCATTATTACGATTTAAATGTG CAAAAAACAGAACAACAATTGAAAAACTATT ATCAAAATTGCTATGTATTTCAGCTGCAAGTA TGCTAATACAACCACCAAAATTACGTAGTACC CCAGCTGCGTTATATTGGTTTAAAACAGCAAT GTCAAATATTAGTGAGGTTGATGGAGAAACA CCAGAATGGATTCAAAGACAAACAGTATTAC AGCATAGTTTTAATGATGCAATATTTGACCTA TCTGAAATGGTACAATGGGCATATGACAATG ATTTTATAGATGATAGTGATATAGCATATAAA TATGCACAATTGGCAGAAACTAATAGTAATG CATGTGCTTTTTTAAAAAGTAATTCGCAAGCT AAAATTGTAAAAGATTGTGCAACAATGTGTA GACATTATAAACGAGCTGAAAAAAGAGAAAT GACAATGTCACAGTGGATTAAAAGGCGATGT GAAAAGGTGGACGATGACGGTGACTGGAGG GACATAGTACGATTTTTAAGATATCAACAAGT AGATTTTGTGGCATTTTTATCTGCACTAAAAA ATTTTTTACATGGTGTGCCTAAAAAAAATTGC ATACTTATATATGGAGCACCAAACACAGGTA AATCATTATTTGGAATGAGTCTAATGCATTTC TTACAAGGAGCTATTATATCCTATGTAAATTC TAAAAGCCATTTTTGGTTGCAGCCATTATATG ATGCCAAAATAGCTATGTTAGATGATGCTAC ATCGCCATGTTGGGCATATATAGACCAATATT TAAGAAATGCACTAGATGGAAATCCTATTTCA TTAGATGTAAAGCATAAAGCATTAGTGCAAT TAAAATGCCCACCTTTACTTATTACATCAAAT ATAAATGCAGGCAAAGATGACAGGTGGCCAT ACTTACATAGCAGGGTAGTGGTCTTTACATTT CACAATGAATTCCCATTTGATAAAAATGGAA ACCCAGTGTATGGGCTTAATGATAAAAACTG GAAATCCTTTTTCTCAAGGACGTGGTGCAGA TTAAATTTGCACGAGGAAGAGGACAAAGAA AATGATGGAGACGCTTTCCCAGCGTTTAAGT GTGTGTCAGGACAAAATACTAGAACATTACG AGACTGA ATGGCTGACCCCGCAGGGACCGATGAGGGA GAAGGCACAGGATGTAATGGATGGTTTTTCG TGGAGGCAGTCGTGAGCAGGAGAACAGGAA GCTCCGTGGAAGATGAGAACGAAGACGATT GCGACCGGGGCGAGGATATGGTGGACTTTA TCAATGATACTGACATCCTGAACATTCAGGCC GAGACCGAAACAGCTCAGGCACTGTTCCACG CCCAGGAGGAACAGACCCATAAGGAGGCTG TCCAGGTGCTGAAGAGGAAATATGCATCTAG TCCACTGTCAAGCGTCAGCCTGTGCGTGAAC AATAACATCTCCCCCCGCCTGAAGGCCATCTG TATTGAGAATAAGAACACTGCCGCTAAACGG AGACTGTTCGAACTGCCTGATTCCGGCTACG GGAATTCTGAGGTGGAAATCCACGAGATTCA GCAGGTCGAAGGGCATGATACTGTGGAGCA GTGCAGTATGGGATCAGGCGACAGCATTACC TCCTCTAGTGATGAGCGGCACGACGAAACTC CAACCAGAGACATCATTCAGATCCTGAAGTG TTCCAATGCAAACGCAGCCATGCTGGCCAAG TTCAAAGAGCTGTTTGGCATCTCTTTCACAGA ACTGATTAGGCCCTTCAAGTCCGATAAATCTA CATGCACTGACTGGTGTGTCGCTGCATTTGG GATTGCACCTAGCGTGGCCAACTTCAAGCAC ATCACCTACGTCTATATCTACAATGTCTATCG CGTGCATGGAGCCATGGTCATCCTGGCTCTG CTGCGCTTTAAGGTCGAGAAACGAGAACAGC AGCTGAAGACAATCGATGCCAAACTGCTGTG TATTAGTGCCGCTTCAATGCTGATCCAGCCAC CTAAGCTGCGATCCACCCCAGCAGCCCTGTAT TGGTTCAAAACAGCCATGAGCAACATTTCCG AGGTGGACGGCGAGACACCCGAATGGATCC AGAGACAGACTGTCCTGCAGCACTCTTTTAAC GATGCCATCTTCGACCTGAGTGAGATGGTGC AGTGGGCTTACGATAATGACTTTATCGACGA TTCAGATATTGCCTATAAGTACGCACAGCTGG CCGAAACCAATAGCAACGCCTGCGCTTTCCTG AAATCAAATAGCCAGGCTAAGATCGTGAAAG ACTGCGCAACAATGTGTCGCCACTACAAGCG AGCAGAGAAACGGGAAATGACTATGTCTCAG TGGATTAAGAGGCGATGTGCACAGGTGGAC GATGACGGCGATTGGAGGGACATCGTCCGA TTTCTGCGGTATCAGCAGGTCGATTTCGTGGC TTTTCTGAGCGCACTGAAGAATTTCCTGCATG GGGTGCCCAAGAAAAACTGCATCCTGATCTA CGGGGCTCCTAATACCGGAAAAAGTCTGTTT GGCATGTCACTGATGCACTTCCTGCAGGGAG CCATCATTAGTTATGTGAACTCCAAGTCTCAT TTTTGGCTGCAGCCCCTGTACGACGCTAAAAT TGCAATGCTGGATGACGCCACCAGCCCATGC GGCATCTATCGACCCATTTTTAAGAAATGTAC ACGGTGGAAGTCTTACATCAGTTTCAGATGT AAAGCTCTGAGCATCGTGCACATTATGCCTAC CTTCACATACTATATCAATATTAACGCCGGCA AGGATGACAGGTGGCCATATCTGCACTCCCG CGTGGTCGTGTTCACATTCCATAACGAGTTCC CTTTCGATAAGAATGGGAACCCAGAATACGG ACTGAATGACAAGAACTGGAAATCATTCTTTA GCAGAACTTGGTGCAGGCTGAACCTGCATGA GGAAGAGGTGAAGGAGAATGATGGCGACGC CTTCCCTGCTTTTAAATGTGTGTCTGGGCAGA ATACTAGAACCCTGAGGGAC MADPAGTDEGEGTGCNGWFFVEA VVSRRTGDPVSEDENEDDCDRGED MVDFINDTDILNIQAETETAQALFHA QEEQTHKEAVQVLKRKYASSPLSSVS LCVNNNISPRLKAICIENKNTAAKRRL FELPDSGYGNSEVEIQQIQQVEGHD TVEQCSMGSGDSITSSSDERHDETPT RDIIQILKCSNANAAMLAKFKELFGIS FTELIRPFKSDKSTCTDWCVAAFGIA PSVAESLKTLIKPYCLYIHIQCLSCSWG MVILALLRFKCAKNRTTIEKLLSKLLCI SAASMLIQPPKLRSTPAALYWFKTA MSNISEVDGETPEWIQRQTVLQHSF NDAIFDLSEMVQWAYDNDFIDDSDI AYKYAQLAETNSNACAFLKSNSQAKI VKDCATMCRHYKRAEKREMTMSQ WIKRRCEKVDDDGDWRDIVRFLRY QQVDFVAFLSALKNFLHGVPKKNCIL IYGAPNTGKSLFGMSLMHFLQGAIIS YVNSKSHFWLQPLYDAKIAMLDDAT SPCWAYIDQYLRNALDGNPISLDVK HKALVQLKCPPLLITSNINAGKDDRW PYLHSRVVVFTFHNEFPFDKNGNPV YGLNDKNWKSFFSRTWCRLNLHEEE DKENDGDAFPAFKCVSGQNTRTLRD Page 25 of 48 % Nucleotide Change 27.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV35 E2 ATGATGGAGACGCTTTCCCAGCGTTTAAGTG TGTGTCAGGACAAAATACTAGAACATTACGA GACTGATAGCACATGTTTGTCTGATCACATAC AGTATTGGAAACTGATTCGTCTTGAATGTGC AGTATTTTATAAAGCAAGAGAAATGGGAATT AAAACTCTTAACCACCAAGTGGTTCCAACGCA GGCCATTTCAAAAGCCAAAGCAATGCAAGCA ATTGAACTGCAATTAATGTTAGAGACATTAAA TACAACTGAGTATAGCACAGAAACATGGACA CTGCAAGAAACAAGTATTGAATTATATACAA CAGTTCCACAAGGATGTTTTAAAAAACATGG GGTTACAGTGGAAGTACAATTTGATGGTGAT AAACAAAATACTATGCATTATACTAATTGGAC ACATATATATATATTAGAGGACAGTATATGTA CTGTTGTAAAGGGACTGGTAAATTATAAAGG TATTTATTATGTGCATCAGGGTGTAGAAACAT ATTATGTTACTTTTAGGGAAGAGGCTAAAAA GTATGGAAAAAAAAATATATGGGAAGTGCAT GTGGGTGGTCAGGTAATTGTTTGTCCTGAAT CTGTATTTAGCAGCACAGAACTATCCACTGCT GAAATTGCTACACAGCTACACGCCTACAACA CCACCGAGACCCATACCAAAGCCTGCTCCGT GGGCACCACAGAAACCCAGAAGACAAATCAC AAACGACTTCGAGGGGGTACCGAGCTCCCCT ACAACCCCACCAAGCGAGTGCGACTCAGTGC CGTGGACAGTGTTGACAGAGGGGTCTACTCT ACATCTGACTGCACAAACAAAGACCGGTGTG GTAGTTGTAGTACAACTACACCTATAGTACAT TTAAAAGGTGATGCAAATACATTAAAGTGTTT AAGATATAGATTGGGTAAATATAAAGCATTG TATCAAGATGCTTCATCTACATGGAGATGGA CATGTACAAACGATAAAAAACAAATAGCAAT TGTAACATTAACTTACACAACAGAATATCAAA GGGATAAATTTTTAACTACAGTAAAAATACCT AACACAGTTACAGTGTCTAAAGGATATATGT CTATATGA TTGTTTGTCCTGAATCTGTATTTAGCAGCACA GAACTATCCACTGCTGAAATTGCTACACAGCT ACACGCCTACAACACCACCGAGACCCATACC AAAGCCTGCTCCGTGGGCACCACAGAAACCC AGAAGACAAATCACAAACGACTTCGAGGGG GTACCGAGCTCCCCTACAACCCCACCAAGCG AGTGCGACTCAGTGCCGTGGACAGTGTTGAC AGAGGGGTCTACTCTACATCTGACTGCACAA ACAAAGACCGGTGTGGTAGTTGTAGTACAAC TACACCTATAG ATGATAGACCTTACAGCTTCCAGTACTGTGTT GCTGTGCTTTTTGTTGTGCTTTTGTGTGCTTTT GTGCTTGTGTCTGCTTGTACGTTCGCTATTGC TATCTGTGTCATTATACTCAGCATTAATATTAC TGGTTTTAATACTGTGGGTTACTGTAGCAACA CCACTACGTTGCTTTTGTTGTTTTCTTTGCTTT TTGTATATACCTATGGGAATGATTAACGCTCA TGCACAATATTTGGCAGTACAGTAA ATGTTTCAGGACCCAGCTGAACGACCTTACA AACTGCATGATTTGTGCAACGAGGTAGAAGA AAGCATCCATGAAATTTGTTTGAATTGTGTAT ACTGCAAACAAGAATTACAGCGGAGTGAGGT ATATGACTTTGCATGCTATGATTTGTGTATAG TATATAGAGAAGGCCAGCCATATGGAGTATG CATGAAATGTTTAAAATTTTATTCAAAAATAA GTGAATATAGATGGTATAGATATAGTGTGTA TGGAGAAACGTTAGAAAAACAATGCAACAAA CAGTTATGTCATTTATTAATTAGGTGTATTAC ATGTCAAAAACCGCTGTGTCCAGTTGAAAAG CAAAGACATTTAGAAGAAAAAAAACGATTCC ATAACATCGGTGGACGGTGGACAGGTCGGT GTATGTCCTGTTGGAAACCAACACGTAGAGA AACCGAGGTGTAA ATGATGGAAACTCTGTCACAGCGACTGAGCG TCTGCCAGGATAAGATTCTGGAGCATTACGA AACCGATAGCACTTGTCTGAGCGACCACATC CAGTACTGGAAGCTGATTAGACTGGAGTGCG CTGTGTTCTATAAGGCAAGGGAAATGGGGAT CAAAACTCTGAACCATCAGGTGGTCCCAACC CAGGCAATCAGCAAGGCCAAAGCTATGCAG GCCATTGAGCTGCAGCTGATGCTGGAAACCC TGAATACCACAGAGTACTCAACCGAAGACTG GACACTGCAGGAGACTAGCATTGAACTGTAC ACTACCGTGCCCACACGGTGCCTGAAGAAAG ATGTGTATACTGTCGAGGCTCAGTTTGACGG CGATAAGCAGAACACCATGCACTACACTAAT TGGACCCATATCTATATTCTGGAAGACAGTAT CTGTACAGTGGTCAAGGGGCTGGTGAACTAC AAAGGAATCTACTATGTGCACCAGGGCGTCG AGACCTACTATGTCACATTCAGAGAGGAAGC CAAGAAATATGGGAAGAAAAATATCTGGGA GGTGCATGTCGGCGGGCAGGTCATCGTCTGC CCCGAATCTGTGTTTAGCTCCACTGAGCTGAG TACCGCAGAAATCGCCACCCAGCTGCACGCT TACAACACAACTGAGACCCATACAAAGGCAT GTTCCGTGGGAACCACAGAAACACAGAAGAC TAACCACAAACGGCTGAGAGGAGGCACCGA GCTGCCCTACAATCCTACAAAGAGGGTGCGC CTGAGTGCCGTGGACTCAGTCGATCGCGGCG TCTATTCAACAAGCGACTGTACTAACAAAGAT CGATGCGGCTCCTGTTCTACTACCACACCTAT CGTGCATCTGAAGGGGGACGCTAATACCCTG AAATGCTCTCGATACCGGCTGGGAAAGTACA AAGCCCTGTATCAGGACGCTTCTAGTACATG GAGGTGGACTTGTACCAACGATAAGAAACAG ATCGCAATTGTGACACTGACTTACACTACCGA GTATCAGCGCGATAAGTTCCTGACAACTGTG AAAATCCCAAATACCGTGACAGTCAGCAAGG GCTATATGTCCATT ATGTTTGTCCTGAACCTGTACCTGGCTGCCCA GAACTATCCCCTGCTGAAGCTGCTGCATTCCT ATACCCCTACCACTCCTCCACGGCCAATCCCC AAGCCTGCCCCATGGGCTCCCCAGAAACCTC GGAGACAGATTACCAACGACTTCGAGGGAGT GCCAAGCTCCCCAACCACACCACCTTCTGAGT GCGATAGTGTCCCTTGGACAGTGCTGACTGA AGGCTCCACCCTGCACCTGACAGCCCAGACT AAGACCGGGGTGGTCGTGGTCGTGCAGCTG CATCTG ATGATTGACCTGACTGCTTCCTCCACTGTGCT GCTGTGTTTTCTGCTGTGTTTCTGCGTCCTGCT GTGCCTGTGTCTGCTGGTGCGGTCTCTGCTGC TGAGCGTGTCCCTGTACAGTGCTCTGATCCTG CTGGTCCTGATTCTGTGGGTGACCGTCGCAA CACCCCTGCTGGCCTTCGTGGTCTCCTGCTTT TGTATCTACCTGTGGATGATTAACGCCCACGC TCAGTATCTGGCCGTGCAG ATGTTCCAGGACCCCGCCGAAAGGCCCTATA AACTGCACGATCTGTGTAACGAAGTCGAAGA GAGCATCCACGAAATCTGTCTGAATTGCGTG TACTGTAAGCAGGAGCTGCAGCGCTCTGAAG TCTACGACTTCGCCTGCTATGATCTGTGTATC GTGTACCGAGAGGGACAGCCATATGGCGTCT GCATGAAGTGTCTGAAGTTCTACAGCAAGAT CTCCGAATACAGGTGGTACCGCTATAGTGTG TATGGGGAGACTCTGGAAAAGCAGTGCAAC AAACAGCTGTGTCACCTGCTGATCAGGTGCA TTACCTGTCAGAAGCCCCTGTGCCCTGTCGAG AAACAGAGACACCTGGAGGAAAAGAAAAGG TTCCATAATATCGGAGGACGATGGACAGGAC GATGCATGTCCTGTTGGAAGCCCACCCGGAG AGAGACAGAAGTG MMETLSQRLSVCQDKILEHYETDST CLSDHIQYWKLIRLECAVFYKAREMG IKTLNHQVVPTQAISKAKAMQAIELQ LMLETLNTTEYSTETWTLQETSIELYT TVPQGCFKKHGVTVEVQFDGDKQN TMHYTNWTHIYILEDSICTVVKGLVN YKGIYYVHQGVETYYVTFREEAKKYG KKNIWEVHVGGQVIVCPESVFSSTEL STAEIATQLHAYNTTETHTKACSVGT TETQKTNHKRLRGGTELPYNPTKRV RLSAVDSVDRGVYSTSDCTNKDRCG SCSTTTPIVHLKGDANTLKCLRYRLGK YKALYQDASSTWRWTCTNDKKQIAI VTLTYTTEYQRDKFLTTVKIPNTVTVS KGYMSI HPV35 E4 HPV35 E5 HPV35 E6 Page 26 of 48 % Nucleotide Change 25.7% LFVLNLYLAAQNYPLLKLLHSYTPTTP PRPIPKPAPWAPQKPRRQITNDFEG VPSSPTTPPSECDSVPWTVLTEGSTL HLTAQTKTGVVVVVQLHL 25.6% MIDLTASSTVLLCFLLCFCVLLCLCLLV RSLLLSVSLYSALILLVLILWVTVATPL RCFCCFLCFLYIPMGMINAHAQYLA VQ 28.1% MFQDPAERPYKLHDLCNEVEESIHEI CLNCVYCKQELQRSEVYDFACYDLCI VYREGQPYGVCMKCLKFYSKISEYR WYRYSVYGETLEKQCNKQLCHLLIRC ITCQKPLCPVEKQRHLEEKKRFHNIG GRWTGRCMSCWKPTRRETEV 24.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV35 E7 ATGCATGGAGAAATAACTACATTGCAAGACT ATGTTTTAGATTTGGAACCCGAGGCAACTGA CCTATACTGTTATGAGCAATTGTGTGACAGCT CAGAGGAGGAGGAAGATACTATTGACGGTC CAGCTGGACAAGCAAAACCAGACACCTCCAA TTATAATATTGTAACGTCCTGTTGTAAATGTG AGGCGACACTACGTCTGTGTGTACAGAGCAC ACACATTGACATACGTAAATTGGAAGATTTAT TAATGGGCACATTTGGAATAGTGTGCCCCGG CTGTTCACAGAGAGCATAA ATGTCTCTGTGGCGGTCTAACGAAGCCACTG TCTACCTGCCTCCAGTGTCAGTGTCTAAGGTT GTTAGCACTGATGAATATGTAACACGCACAA ACATCTACTATCATGCAGGCAGTTCTAGGCTA TTAGCTGTGGGTCACCCATACTATGCTATTAA AAAACAAGATTCTAATAAAATAGCAGTACCC AAGGTATCTGGTTTGCAATACAGAGTATTTA GAGTAAAATTACCAGATCCTAATAAGTTTGG ATTTCCAGACACATCATTTTATGATCCTGCCTC CCAGCGTTTGGTTTGGGCCTGTACAGGAGTT GAAGTAGGTCGTGGTCAGCCATTGGGTGTAG GTATTAGTGGTCATCCTTTATTAAATAAATTG GATGATACTGAAAATTCTAATAAATATGTTGG TAACTCTGGTACAGATAACAGGGAATGCATT TCTATGGATTATAAACAAACACAATTGTGTTT AATAGGTTGTAGGCCTCCTATAGGTGAACAT TGGGGAAAAGGCACACCTTGTAATGCTAACC AGGTAAAAGCAGGAGAATGTCCTCCTTTGGA GTTACTAAACACTGTACTACAAGACGGGGAC ATGGTAGACACAGGATTTGGTGCAATGGATT TTACTACATTACAAGCTAATAAAAGTGATGTT CCCCTAGATATATGCAGTTCCATTTGCAAATA TCCTGATTATCTAAAAATGGTTTCTGAGCCAT ATGGAGATATGTTATTTTTTTATTTACGTAGG GAGCAAATGTTTGTTAGACATTTATTTAATAG GGCTGGAACTGTAGGTGAAACAGTACCTGCA GACCTATATATTAAGGGTACCACTGGCACATT GCCTAGTACTAGTTATTTTCCTACTCCTAGTG GCTCTATGGTAACCTCCGATGCACAAATATTT AATAAACCATATTGGTTGCAACGTGCACAAG GCCATAATAATGGTATTTGTTGGAGTAACCA ATTGTTTGTTACTGTAGTTGATACAACCCGTA GTACAAATATGTCTGTGTGTTCTGCTGTGTCT TCTAGTGACAGTACATATAAAAATGACAATTT TAAGGAATATTTAAGGCATGGTGAAGAATAT GATTTACAGTTTATTTTTCAGTTATGTAAAAT AACACTAACAGCAGATGTTATGACATATATTC ATAGTATGAACCCGTCCATTTTAGAGGATTG GAATTTTGGCCTTACACCACCGCCTTCTGGTA CCTTAGAGGACACATATCGCTATGTAACATCA CAGGCTGTAACTTGTCAAAAACCCAGTGCAC CAAAACCTAAAGATGATCCATTAAAAAATTAT ACTTTTTGGGAGGTTGATTTAAAGGAAAAGT TTTCTGCAGACTTAGATCAATTTCCGTTGGGC CGTAAATTTTTGTTACAAGCAGGACTAAAGG CCAGGCCTAATTTTAGATTAGGCAAGCGTGC AGCTCCAGCATCTACATCTAAAAAATCTTCTA CTAAACGTAGAAAAGTAAAAAGTTAA ATGCACGGCGAAATTACCACTCTGCAGGATT ATGTCCTGGATCTGGAGCCCGAAGCCACTGA CCTGTATTGTTATGAGCAGCTGTGTGACAGCT CCGAGGAAGAGGAAGACACAATCGATGGAC CAGCAGGACAGGCTAAGCCTGATACCTCTAA CTACAATATTGTGACAAGTTGCTGTAAATGCG AGGCAACTCTGCGGCTGTGTGTCCAGAGCAC CCACATCGACATTAGAAAGCTGGAAGATCTG CTGATGGGAACCTTCGGCATCGTGTGCCCCG GGTGTTCTCAGAGGGCC ATGAGCCTGTGGAGGAGCAATGAAGCAACC GTCTATCTGCCCCCTGTGAGCGTGAGCAAAG TCGTGAGCACTGATGAATACGTGACAAGGAC CAACATCTACTATCACGCAGGAAGCTCCCGA CTGCTGGCTGTGGGGCATCCTTACTATGCAAT CAAGAAACAGGACTCCAACAAGATTGCCGTG CCAAAAGTCTCTGGACTGCAGTACAGAGTGT TCAGGGTCAAGCTGCCTGATCCAAACAAGTT CGGCTTTCCTGACACTTCCTTTTATGATCCATG CCTGCAGCGACTGGTGTGGGCCTGTACCGGA GTGGAGGTCGGACGAGGACAGCCACTGGGA GTCGGCATCTCTGGACACCCTCTGCTGAACAA GCTGGACGATACCGAGAACCTGAACAAGTAC GTGGGAAACAGCGGCAATTCCGGGACCGAC AATCGGGAATGCATTAGCATGGATTATAAGC AGACACAGCTGTGCCTGATCGGATGTAGACC CCCTATTGGCGAACATTGGGGGAAGGGAAC ACCCTGCAACGCTAATCAGGTGAAAGCAGGC GAGTGTCCACCCCTGGAACTGCTGAACACAG TGCTGCAGGACGGGGATATGGTCGACACTG GCTTCGGGGCCATGGATTTTACCACACTGCA GGCTAATAAGTCTGACGTGCCTCTGGATATCT GCTCTAGTATTTGTAAGTACCCAGACTATCTG AAAATGGTCAGTGAGCCCTACGGGGATATGC TGTTCTTTTATCTGCGGAGAGAACAGATGTTC GTGCGGCACCTGTTTAACAGAGCTGGAACTG TGGGCGAGACCGTCCCAGCAGACCTGTACAT CAAGGGGACTACCGGAACACTGCCCTCAACT AGCTATTTCCCCACCCCTTCCGGCTCTATGGT GACATCCGATGCCCAGATCTTCAACAAGCCTT ACTGGCTGCAGAGGGCTCAGGGCCATAACAA TGGGATTTGCTGGAGCAACCAGCTGTTCGTG ACTGTGGTCGACACAACTCGCTCCACCAATAT GTCTGTGTGTAGTGCTGTCTCAAGCTCCGACT CTACCTACAAGAACGATAACTTCAAGGAGTA CCTGAGACACGGCGAGGAATATGACCTGCAG TTCATCTTTCAGCTGTGCAAGATTACCCTGAC AGCCGATGTGATGACATATATCCATTCAATGA ACCCAAGCATTCTGGAGGACTGGAATTTCGG GCTGACTCCTCCACCCAGCGGAACCCTGGAA GATACATACAGATATGTGACTAGTCAGGCAG TCACCTGTCAGAAGCCTTCAGCCCCAAAGCCC AAAGACGATCCACTGAAAAACTACACATTCT GGGAGGTGGACCTGAAGGAAAAGTTCAGCG CAGACCTGGATCAGTTCCCCCTGGGACGGAA GTTTCTGCTGCAGGCAGGCCTGAAAGCACGA CCAAATTTCCGACTGGGAAGGCGAGCAGCTC CTGCAAGTACATCAAAGAAATCTAGTACTAA GCGACGGAAGGTGAAAAGC MHGEITTLQDYVLDLEPEATDLYCYE QLCDSSEEEEDTIDGPAGQAKPDTS NYNIVTSCCKCEATLRLCVQSTHIDIR KLEDLLMGTFGIVCPGCSQRA HPV35 L1 Page 27 of 48 MSLWRSNEATVYLPPVSVSKVVSTD EYVTRTNIYYHAGSSRLLAVGHPYYAI KKQDSNKIAVPKVSGLQYRVFRVKLP DPNKFGFPDTSFYDPASQRLVWACT GVEVGRGQPLGVGISGHPLLNKLDD TENSNKYVGNSGTDNRECISMDYKQ TQLCLIGCRPPIGEHWGKGTPCNAN QVKAGECPPLELLNTVLQDGDMVD TGFGAMDFTTLQANKSDVPLDICSSI CKYPDYLKMVSEPYGDMLFFYLRRE QMFVRHLFNRAGTVGETVPADLYIK GTTGTLPSTSYFPTPSGSMVTSDAQI FNKPYWLQRAQGHNNGICWSNQL FVTVVDTTRSTNMSVCSAVSSSDSTY KNDNFKEYLRHGEEYDLQFIFQLCKIT LTADVMTYIHSMNPSILEDWNFGLT PPPSGTLEDTYRYVTSQAVTCQKPSA PKPKDDPLKNYTFWEVDLKEKFSADL DQFPLGRKFLLQAGLKARPNFRLGK RAAPASTSKKSSTKRRKVKS % Nucleotide Change 23.2% 27.7% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV35 L2 ATGCGACACAAAAGGTCTACAAAACGTGTTA AACGTGCATCTGCAACACAACTATATCGTACT TGCAAAGCTGCAGGAACTTGTCCACCAGATG TTATACCTAAGGTTGAGGGTAATACTGTTGCT GATCAAATTTTAAAATATGGCAGCATGGCTG TGTTTTTTGGGGGGTTAGGAATTGGTTCTGG ATCTGGCACAGGTGGAAGATCTGGATATGTT CCACTGGGTACAACACCTCCAACGGCTGCCA CAAACATTCCTATACGACCCCCTGTAACTGTG GAAAGTATACCATTAGACACAATTGGCCCTTT AGATTCTTCTATAGTGTCATTAGTAGAGGAAA CTAGTTTTATTGAGTCTGGTGCCCCTGTTGTT ACACCAAGGGTCCCACCTACAACAGGTTTTAC AATAACCACATCTACAGATACCACACCTGCTA TTTTAGATGTGACATCCATAAGTACACATGAT AATCCTACTTTCACTGATCCTTCTGTTTTACAC CCACCCACGCCTGCAGAAACTTCAGGTCATTT TGTACTTTCATCATCTTCTATTAGTACACATAA TTATGAAGAAATCCCTATGGATACTTTTATTG TTTCCACAGACAGCAATAATATAACTAATAGC ACGCCTATTCCAGGGTCTCGCCCTACGACAC GCCTAGGATTATATAGTAAAGGTACCCAGCA GGTTAAGGTTGTTGACCCTGCCTTTATGACTT CTCCTGCAAAACTTATTACATATGATAATCCT GCATATGAAGGCCTTAACCCTGATACAACCTT ACAATTTGAGCATGAGGATATTAGCTTAGCTC CGGATCCTGACTTTATGGACATTATAGCTTTA CATAGGCCTGCACTAACATCTAGGAAAGGCA CTATTAGATATAGTAGAGTAGGTAATAAACG TACTATGCATACACGAAGTGGAAAAGCTATA GGGGCACGGGTACATTATTATCAGGATTTAA GTAGTATTACTGAAGATATAGAATTACAACCC TTACAACATGTACCATCCTCTTTACCACATACC ACTGTTTCAACATCATTAAATGATGGTATGTT TGATATTTATGCTCCTATAGATACTGAGGAAG ATATTATATTTTCAGCATCTTCTAACAATACTT TATATACTACATCTAACACTGCATATGTTCCTA GCAATACTACTATACCATTAAGTAGTGGCTAT GATATTCCTATAACAGCAGGGCCAGACATTG TATTTAACTCTAATACTATTACTAACACTGTAC TACCGGTACCCACAGGTCCTATATATTCTATT ATTGCAGATGGGGGTGACTTTTATTTACACCC TAGTTATTATTTATTAAAACGACGTCGTAAAC GTATCCCATATTTTTTTGCAGATGTCTCTGTG GCGGTCTAA ATGAGACATAAAAGAAGCACAAAGAGAGTC AAGAGAGCAAGCGCAACACAGCTGTACCGA ACCTGCAAAGCCGCCGGAACATGCCCTCCAG ACGTCATCCCCAAGGTGGAGGGAAACACCGT CGCTGATCAGATTCTGAAATACGGCTCCATG GCAGTGTTCTTTGGAGGACTGGGAATCGGAT CAGGAAGCGGAACAGGAGGACGATCTGGCT ATGTGCCACTGGGAACCACACCACCTACAGC AGCTACTAATATCCCCATTCGGCCACCCGTGA CCGTCGAGTCTATCCCCCTGGACACAATTGGC CCTCTGGATAGCTCCATCGTCAGTCTGGTGG AGGAAACTTCTTTCATTGAAAGTGGGGCCCC TGTGGTCACCCCAAGAGTGCCTCCAACTACC GGCTTCACCATCACAACTAGCACCGACACCAC ACCCGCCATCCTGGATGTGACATCCATTTCTA CTCACGACAACCCAACCTTCACAGATCCATCT GTCCTGCACCCACCTACCCCAGCAGAGACAA GTGGCCATTTTGTGCTGTCTAGTTCAAGCATC TCAACCCATAACTACGAGGAAATCCCTATGG ACACATTCATTGTGAGCACTGATTCCAACAAT ATCACCAATTCAACACCAATTCCCGGGAGCC GGCCTACTACCAGACTGGGACTGTATAGCAA GGGCACCCAGCAGGTGAAAGTGGTCGACCC AGCCTTCATGACTAGCCCCGCCAAGCTGATCA CCTACGATAACCCCGCATATGAAGGCCTGAA TCCTGACACAACTCTGCAGTTCGAGCACGAA GATATTAGCCTGGCCCCTGACCCAGATTTTAT GGACATCATTGCTCTGCATCGACCAGCACTG ACCAGCAGGAAAGGGACAATCCGCTACTCCC GAGTCGGAAACAAGAGGACTATGCACACCC GCAGCGGGAAAGCAATTGGAGCCAGGGTGC ATTACTATCAGGACCTGTCCTCTATCACCGAG GATATTGAACTGCAGCCACTGCAGCACGTCC CCAGTTCACTGCCTCATACCACAGTGAGTACA TCACTGAATGACGGCATGTTCGATATCTACGC CCCCATTGACACTGAGGAAGATATCATCTTCA GCGCTAGCTCCAACAATACACTGTACACTACC AGTAACACTGCTTATGTGCCTTCAAATACAAC TATCCCACTGTCTAGTGGCTATGACATCCCTA TTACCGCAGGGCCAGATATCGTGTTCAACTCC AATACTATTACCAATTCTGTCCTGCCCGTGCC TACAGGCCCTATCTACAGCATCATTGCCGACG GGGGAGATTTTTATCTGCACCCTTCCTACTAT CTGCTGAAGCGGAGAAGGAAAGCTATTCCAT ACTTCTTTGCCGACGTGTCTGTCGCTGTG MRHKRSTKRVKRASATQLYRTCKAA GTCPPDVIPKVEGNTVADQILKYGS MAVFFGGLGIGSGSGTGGRSGYVPL GTTPPTAATNIPIRPPVTVESIPLDTIG PLDSSIVSLVEETSFIESGAPVVTPRVP PTTGFTITTSTDTTPAILDVTSISTHDN PTFTDPSVLHPPTPAETSGHFVLSSSS ISTHNYEEIPMDTFIVSTDSNNITNST PIPGSRPTTRLGLYSKGTQQVKVVDP AFMTSPAKLITYDNPAYEGLNPDTTL QFEHEDISLAPDPDFMDIIALHRPAL TSRKGTIRYSRVGNKRTMHTRSGKAI GARVHYYQDLSSITEDIELQPLQHVP SSLPHTTVSTSLNDGMFDIYAPIDTEE DIIFSASSNNTLYTTSNTAYVPSNTTIP LSSGYDIPITAGPDIVFNSNTITNTVLP VPTGPIYSIIADGGDFYLHPSYYLLKRR RKRIPYFFADVSVAV Page 28 of 48 % Nucleotide Change 28.9% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV39 E1 ATGGCCAATCGTGAAGGTACAGACGGGGAT GGGTCGGGATGTAACGGATGGTTTCTAGTAC AGGCAATAGTAGATAAACAAACAGGCGACA CAGTGTCGGAGGATGAGGATGAAAATGCAA CAGATACAGGTTCAGACCTGGCAGACTTTATT GATGATTCCACAGATATTTGTGTACAGGCAG AGCGTGAGACAGCACAGGTACTTTTACATAT GCAAGAGGCCCAAAGGGATGCACAAGCAGT GCGTGCCTTAAAACGAAAGTATACAGACAGC AGTGGCGACACTAGACCGTATGGAAAAAAA GTAGGCAGGAATACCAGGGGAACACTACAG GAAATTTCATTAAATGTAAGCAGTACGCAGG CAACACAAACGGTGTATTCCGTGCCAGACAG CGGATATGGCAATATGGAAGTGGAAACAGCT GAAGTGGAGGAGGTAACTGTAGCAACTAAT ACAAATGGGGATGCTGAAGGGGAACATGGC GGCAGTGTACGGGAGGAGTGCAGTAGTGTG GATAGTGCTATAGATAGTGAAAACCAGGATC CCAAATCTCCAACTGCACAAATTAAATTATTG TTACAATCCAATAACAAAAAGGCTGCAATGC TAACACAATTTAAAGAAACATATGGACTATCC TTTACTGACCTGGTACGTACGTTTAAAAGTGA TAAAACAACATGTACAGACTGGGTGGCAGCC ATATTTGGAGTACATCCAACTATTGCAGAAG GATTTAAAACATTAATCAACAAATATGCCTTA TATACACATATACAAAGCTTAGACACAAAAC AAGGAGTACTAATTTTAATGCTAATAAGATAT ACATGTGGAAAAAATAGGGTTACTGTAGGAA AGGGATTAAGTACATTGTTACATGTTCCAGA AAGTTGTATGCTTCTGGAGCCTCCTAAACTGC GCAGCCCTGTAGCAGCACTATATTGGTATCG CACAGGTATATCCAATATTAGTGTGGTAACA GGGGATACGCCAGAATGGATACAACGATTAA CTGTTATACAACATGGAATAGATGATAGTGT ATTTGACCTATCGGACATGGTACAATGGGCA TTTGACAATGAATATACTGATGAAAGTGACA TAGCATTTAATTATGCAATGTTAGCAGATTGT AACAGTAATGCTGCAGCCTTTTTAAAAAGTAA CTGCCAGGCAAAATATGTAAAAGATTGTGCA ACAATGTGTAAACATTACAAGCGAGCACAAA AAAGGCAAATGTCCATGTCTCAATGGATAAA ATTTAGGTGTAGTAAATGTGATGAAGGCGGG GACTGGAGACCCATAGTACAATTCTTAAGAT ATCAAGGAATAGAATTTATATCCTTTTTATGT GCATTAAAGGAATTTTTAAAGGGTACTCCCA AAAAAAACTGTATAGTTATATATGGACCTGC GAATACAGGAAAGTCACATTTTTGTATGAGC CTTATGCATTTTTTACAGGGCACAGTTATTTC ATATGTAAACTCCACCAGCCACTTTTGGCTAG AACCACTTGCAGATGCAAAACTAGCAATGTT AGATGATGCAACCGGTACCTGCTGGTCATAT TTCGATAATTATATGAGAAATGCATTAGATG GGTATGCAATAAGTTTAGATAGGAAATATAA AAGTTTACTACAAATGAAATGTCCACCATTAT TAATAACCTCCAATACCAATCCTGTGGAAGAC GATAGGTGGCCATATTTACGTAGTAGGCTAA CAGTGTTTAAATTTCCTAATGCATTTCCATTTG ACCAAAACAGGAATCCAGTGTACACAATCAA TGATAAAAACTGGAAATGTTTTTTTGAAAAG ACTTGGTGCAGATTAGACTTGCAGCAGGACG AGGATGAAGGAGACAATGATGAAAACACTTT CACAACGTTTAAATGTGTTACAGGACAAAAT ACTAGAATACTATGA ATGGCTAATCGGGAGGGGACTGATGGGGAT GGGAGCGGCTGTAATGGCTGGTTCCTGGTGC AGGCAATCGTGGATAAGCAGACTGGCGACA CCGTGAGCGAGGACGAAGATGAGAACGCTA CAGATACTGGCTCCGACCTGGCAGATTTCATC GACGATTCTACAGACATTTGCGTGCAGGCCG AAAGAGAGACTGCTCAGGTCCTGCTGCACAT GCAGGAGGCACAGAGGGATGCACAGGCTGT GCGAGCTCTGAAGCGGAAATACACCGACAGC TCCGGAGATACAAGGCCATATGGAAAGAAA GTGGGCAGGAACACCCGCGGCACACTGCAG GAAATCTCCCTGAATGTCTCTAGTACCCAGGC TACCCAGACAGTGTACTCTGTCCCCGACAGTG GGTATGGAAACATGGAAGTGGAGACTGCCG AGGTCGAGGAAGTGACCGTCGCCACTAACAC CAATGGGGATGCTGAAGGAGAGCATGGCGG GAGCGTGCGGGAGGAATGCTCAAGCGTCGA CTCAGCTATCGATAGCGAAAATCAGGACCCT AAGAGCCCAACAGCCCAGATCAAGCTGCTGC TGCAGTCCAACAATAAGAAAGCCGCTATGCT GACTCAGTTTAAGGAGACCTACGGGCTGAGT TTCACAGATCTGGTGAGAACTTTTAAGTCAGA CAAAACCACATGTACCGATTGGGTGGCAGCC ATCTTCGGAGTCCACCCCACAATTGCAGAGG GCTTTAAGACTCTGATCAACAAATACGCCCTG TATACCCATATTCAGTCTCTGGACACAAAGCA GGGCGTGCTGATCCTGATGCTGATTCGCTAC ACTTGCGGGAAGAATCGAGTGACCGTCGGCA AAGGGCTGTCTACACTGCTGCACGTGCCCGA AAGTTGTATGCTGCTGGAGCCCCCTAAGCTG AGGAGCCCTGTCGCTGCACTGTACTGGTATC GCACAGGGATCAGCAACATTTCCGTGGTCAC TGGAGATACCCCTGAGTGGATCCAGCGGCTG ACTGTGATCCAGCATGGCATTGACGATTCCG TGTTCGACCTGTCTGATATGGTCCAGTGGGCT TTTGACAACGAATACACAGACGAGTCCGATA TTGCATTCAATTATGCTATGCTGGCAGATTGC AACTCAAATGCCGCTGCATTTCTGAAGAGCA ATTGTCAGGCAAAGTACGTGAAAGACTGCGC CACCATGTGTAAGCACTATAAAAGAGCCCAG AAAAGGCAGATGTCCATGTCTCAGTGGATCA AGTTCAGATGCTCTAAATGTGACGAGGGAGG CGATTGGCGGCCTATTGTGCAGTTTCTGAGA TACCAGGGCATCGAATTCATTAGCTTTCTGTG CGCCCTGAAGGAGTTCCTGAAAGGGACTCCT AAGAAAAACTGTATCGTGATCTACGGCCCAG CCAATACCGGGAAGAGTCACTTCTGCATGTC ACTGATGCATTTTCTGCAGGGCACTGTGATCA GCTATGTCAACAGTACCTCACATTTCTGGCTG GAACCACTGGCAGACGCCAAGCTGGCAATGC TGGACGATGCCACAGGAACTTGCTGGTCCTA CTTTGATAACTATATGCGAAATGCTCTGGACG GCTACGCAATCAGCCTGGATCGGAAGTATAA ATCCCTGCTGCAGATGAAGTGTCCACCCCTGC TGATTACATCTAACACTAATCCAGTGGAGGA CGATAGGTGGCCCTACCTGCGGAGTAGACTG ACCGTCTTCAAATTTCCCAACGCCTTCCCTTTT GACCAGAACCGCAATCCCGTGTATACCATCA ACGATAAGAACTGGAAGTGCTTCTTTGAAAA GACATGGTGTCGACTGGACCTGCAGCAGGAC GAAGATGAGGGGGACAACGATGAGAATACC TTCACTACCTTTAAATGTGTGACCGGACAGAA TACACGCATCCTG MANREGTDGDGSGCNGWFLVQAI VDKQTGDTVSEDEDENATDTGSDLA DFIDDSTDICVQAERETAQVLLHMQ EAQRDAQAVRALKRKYTDSSGDTRP YGKKVGRNTRGTLQEISLNVSSTQAT QTVYSVPDSGYGNMEVETAEVEEVT VATNTNGDAEGEHGGSVREECSSV DSAIDSENQDPKSPTAQIKLLLQSNN KKAAMLTQFKETYGLSFTDLVRTFKS DKTTCTDWVAAIFGVHPTIAEGFKTL INKYALYTHIQSLDTKQGVLILMLIRY TCGKNRVTVGKGLSTLLHVPESCMLL EPPKLRSPVAALYWYRTGISNISVVT GDTPEWIQRLTVIQHGIDDSVFDLSD MVQWAFDNEYTDESDIAFNYAMLA DCNSNAAAFLKSNCQAKYVKDCAT MCKHYKRAQKRQMSMSQWIKFRC SKCDEGGDWRPIVQFLRYQGIEFISF LCALKEFLKGTPKKNCIVIYGPANTGK SHFCMSLMHFLQGTVISYVNSTSHF WLEPLADAKLAMLDDATGTCWSYF DNYMRNALDGYAISLDRKYKSLLQM KCPPLLITSNTNPVEDDRWPYLRSRL TVFKFPNAFPFDQNRNPVYTINDKN WKCFFEKTWCRLDLQQDEDEGDND ENTFTTFKCVTGQNTRIL Page 29 of 48 % Nucleotide Change 25.8% % Nucleotide Change 35.4% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV39 E2 ATGAAGGAGACAATGATGAAAACACTTTCAC AACGTTTAAATGTGTTACAGGACAAAATACT AGAATACTATGAACAAGACAGTAAATCAATA TATGATCAAATTAATTATTGGAAATGTGTGCG AATGGAAAATGCAATATTTTATGCAGCACGA GAACGTGGCATGCATACTATTGACCACCAGG TGGTGCCAACCATAAACATTTCAAAATGTAAA GCATATCAAGCTATTGAACTGCAGATGGCAC TAGAAAGTGTTGCACAAACTGAATACAATAC AGAGGAGTGGACATTAAAAGACACTAGTAAT GAACTGTGGCATACACAGCCAAAACAATGTT TTAAAAAACAAGGAACTACAGTGGAGGTGTG GTATGATGGGGACAAATGTAATGCTATGAAC TATGTATTATGGGGTGCTATATATTATAAAAA TAATATAGACATATGGTGTAAAACAGAAGGG TGTGTGGACTATTGGGGTATATATTATATGA ACGAGCACCTAAAAGTATACTATGAAGTGTT TATTCAAGATGCGGAAAGGTATGGGACTAGT GGCAAATGGGAAGTGCATTATAATGGCAACA TAATTCATTGTCCTGACTCTATGTGCAGTACC AGTGACGGATCGGTACCCACTACTGAACTTA CTACCGAATTATCAAACACCACCGCGACCCAT TCCACCGCAACAACCCCATGCACCCAAAAAA CAATCCCGCCGCCGTCTCGAAAGCGACCTCG ACAGTGTGCAGTCACAGAGCCCACTGAGCCC GACGGAGTGTCCCTGGACCATCTTAACAACC CACTCCACAGTAACAGTACAGGCCACAACAC AAGACGGTACCTCAGTTGTGGTAACACTACG CCTATAATACATTTAAAAGGTGACAAAAATG GTTTAAAATGTTTAAGATATAGACTACAAAAA TATGACACATTGTTTGAAAATATTTCATGTAC CTGGCATTGGATACGGGGTAAGGGAACCAA AAACGCTGGCATATTAACTGTTACATATGCCA CAGAGTCACAACGCCAAAAATTTTTGGACAC TGTTAAAATACCTTCTAGTGTACATGTTTCATT GGGTTACATGACATTGTAA TTCATTGTCCTGACTCTATGTGCAGTACCAGT GACGGATCGGTACCCACTACTGAACTTACTAC CGAATTATCAAACACCACCGCGACCCATTCCA CCGCAACAACCCCATGCACCCAAAAAACAAT CCCGCCGCCGTCTCGAAAGCGACCTCGACAG TGTGCAGTCACAGAGCCCACTGAGCCCGACG GAGTGTCCCTGGACCATCTTAACAACCCACTC CACAGTAACAGTACAGGCCACAACACAAGAC GGTACCTCAGTTGTGGTAACACTACGCCTATA A ATGATATTATTGGTATTTTTGGTGTGGTTTGG TGTGTGTATATATATATGTTGCAATGTCCCGC TTTTGCCGTCTGTGCATGTGTGTGCGTATGTG TGGATAATTGTGTTTGTGTTTATTCTTATACGT ACCACACCATTGGAGGTGTTTTTTGTATATTT ACTATTTTTTGTATTGCCCATGTGGTTGTTGC ATAGATGGCAATGGATATGATATAG ATGGCGCGATTTCACAATCCTGCAGAACGGC CATACAAATTGCCAGACCTGTGCACAACGCT GGACACCACCTTGCAGGACATTACAATAGCC TGTGTCTATTGCAGACGACCACTACAGCAAA CCGAGGTATATGAATTTGCATTTAGTGATTTA TATGTAGTATATAGGGACGGGGAACCACTAG CTGCATGCCAATCATGTATAAAATTTTATGCT AAAATACGGGAGCTACGATATTACTCGGACT CGGTGTATGCAACTACATTAGAAAATATAACT AATACAAAGTTATATAATTTATTAATAAGGTG CATGTGTTGTCTGAAACCGCTGTGTCCAGCA GAAAAATTAAGACACCTAAATAGCAAACGAA GATTTCATAAAATAGCAGGAAGCTATACAGG ACAGTGTCGACGGTGCTGGACCACAAAACGG GAGGACCGCAGACTAACACGAAGAGAAACC CAAGTATAA ATGAAGGAAACTATGATGAAAACACTGAGCC AGAGACTGAACGTGCTGCAGGATAAGATTCT GGAGTATTACGAACAGGATTCTAAAAGTATC TACGACCAGATTAACTATTGGAAGTGCGTGC GGATGGAGAATGCCATCTTCTACGCCGCTAG GGAACGCGGGATGCACACTATTGATCATCAG GTGGTCCCAACTTCAAGAAACAGGGAACCAC AGTGGAAGTCTGGTACGACGGCGATAAGTG TAACGCAATGAATTATGTGCTGTGGGGCGCC ATCTACTATAAGAACAATATCGACATTTGGTG CAAAACCGAGGGCTGTGTCGATTATTGGGGG ATCTACTATATGAACGAACATCTGAAGGTGT ACTATGAGGTCTTTATTCAGGATGCCGAACG GTACGGAACTAGCGGCAAATGGGAGGTGCA TTATAACGGCAATATCATTCACTGCCCAGACA GTATGTGTTCTACAAGTGATGGCTCAGTGCC CACTACCGAGCTGACAACTGAACTGTCCAAT ACCACAGCCACTCACTCTACCGCTACTACCCC TTGCACACAGAAGACTATCCCCCCTCCATCAC GAAAACGGCCAAGACAGTGTGCTGTGACCG AGCCCACAGAACCTGACGGCGTCAGCCTGGA TCACCTGAACAATCCCCTGCATTCAAACAGCA CCGGGCACAATACACGGAGATACCTGTCCTG CGGAAACACAACTCCTATCATTCATCTGAAGG GGGACAAAAATGGACTGAAGTGCCTGAGGT ACCGCCTGCAGAAATATGATACACTGTTCGA GAACATCTCTTGTACTTGGCACTGGATTCGAG GCAAGGGGACCAAAAATGCTGGAATCCTGAC CGTGACATACGCAACCGAATCTCAGAGGCAG AAGTTTCTGGACACAGTCAAAATTCCTAGCTC CGTGCACGTCAGTCTGGGCTATATGACACTG MKETMMKTLSQRLNVLQDKILEYYE QDSKSIYDQINYWKCVRMENAIFYA ARERGMHTIDHQVVPTINISKCKAY QAIELQMALESVAQTEYNTEEWTLK DTSNELWHTQPKQCFKKQGTTVEV WYDGDKCNAMNYVLWGAIYYKNNI DIWCKTEGCVDYWGIYYMNEHLKV YYEVFIQDAERYGTSGKWEVHYNGN IIHCPDSMCSTSDGSVPTTELTTELSN TTATHSTATTPCTQKTIPPPSRKRPR QCAVTEPTEPDGVSLDHLNNPLHSN STGHNTRRYLSCGNTTPIIHLKGDKN GLKCLRYRLQKYDTLFENISCTWHWI RGKGTKNAGILTVTYATESQRQKFLD TVKIPSSVHVSLGYMTL ATGATTGTGCTGACACTGTGCGCCGTGCCTG TGACCGACCGCTACCCCCTGCTGAACCTGCTG CCCAACTACCAGACCCCTCCCCGCCCAATCCC ACCTCAGCAGCCACACGCACCCAAGAAACAG AGTCGGAGAAGGCTGGAGTCTGACCTGGAT AGTGTCCAGAGCCAGTCCCCTCTGTCACCAAC CGAATGCCCTTGGACAATTCTGACCACACATA GCACAGTCACTGTGCAGGCTACTACCCAGGA CGGCACTTCCGTGGTCGTGACACTGCGGCTG FIVLTLCAVPVTDRYPLLNLLPNYQTP PRPIPPQQPHAPKKQSRRRLESDLDS VQSQSPLSPTECPWTILTTHSTVTVQ ATTQDGTSVVVTLRL 34.8% ATGATTCTGCTGGTCTTTCTGGTCTGGTTCGG CGTGTGTATCTATATCTGTTGTAATGTCCCTCT GCTGCCATCCGTCCATGTGTGTGCCTACGTGT GGATCATTGTGTTCGTCTTTATCCTGATTCGG ACCACACCCCTGGAGGTGTTCTTTGTCTATCT GCTGTTCTTTGTCCTGCCTATGTGGCTGCTGC ACAGACTGGCTATGGACATGATC ATGGCTAGATTTCATAACCCCGCCGAACGAC CTTACAAACTGCCCGACCTGTGCACTACACTG GATACAACTCTGCAGGACATCACCATCGCCT GCGTGTACTGTCGGAGACCACTGCAGCAGAC TGAGGTCTATGAATTCGCTTTTAGCGACCTGT ACGTGGTCTATAGGGATGGAGAGCCACTGG CAGCTTGCCAGTCTTGTATCAAGTTCTACGCA AAAATTAGGGAGCTGCGCTACTATAGCGACT CCGTGTACGCCACCACACTGGAAAACATCAC AAATACTAAGCTGTATAACCTGCTGATTCGGT GCATGTGCTGTCTGAAGCCCCTGTGTCCTGCT GAAAAACTGAGACACCTGAATTCTAAGAGGC GCTTTCATAAAATTGCAGGCAGTTATACAGG GCAGTGCCGACGGTGTTGGACTACCAAACGG GAGGATAGAAGGCTGACCCGCCGAGAAACA CAGGTC MILLVFLVWFGVCIYICCNVPLLPSVH VCAYVWIIVFVFILIRTTPLEVFFVYLL FFVLPMWLLHRLAMDMI 21.9% MARFHNPAERPYKLPDLCTTLDTTL QDITIACVYCRRPLQQTEVYEFAFSDL YVVYRDGEPLAACQSCIKFYAKIRELR YYSDSVYATTLENITNTKLYNLLIRCM CCLKPLCPAEKLRHLNSKRRFHKIAGS YTGQCRRCWTTKREDRRLTRRETQV 24.8% HPV39 E4 HPV39 E5 HPV39 E6 Page 30 of 48 Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV39 E7 ATGCGTGGACCAAAGCCCACCTTGCAGGAAA TTGTATTAGATTTATGTCCTTACAATGAAATA CAGCCGGTTGACCTTGTATGTCACGAGCAAT TAGGAGAGTCAGAGGATGAAATAGATGAAC CCGACCATGCAGTTAATCACCAACATCAACTA CTAGCCAGACGGGATGAACCACAGCGTCACA CAATACAGTGTTCGTGTTGTAAGTGTAACAAC ACACTGCAGCTGGTAGTAGAAGCCTCACGGG ATACTCTGCGACAACTACAGCAGCTGTTTATG GACTCACTAGGATTTGTGTGTCCGTGTGTGC AACTGCAAACCAGTAA ATGGCTATGTGGCGGTCTAGTGACAGCATGG TGTATTTGCCTCCACCTTCTGTGGCGAAGGTT GTCAATACTGATGATTATGTTACACGCACAG GCATATATTATTATGCTGGCAGCTCTAGATTA TTAACAGTAGGACATCCATATTTTAAAGTGG GTATGAATGGTGGTCGCAAGCAGGACATTCC AAAGGTGTCTGCATATCAATATAGGGTATTTC GCGTGACATTGCCCGATCCTAATAAATTCAGT ATTCCAGATGCATCCTTATATAATCCAGAAAC ACAACGTTTAGTATGGGCTTGTGTAGGGGTG GAGGTGGGCAGGGGCCAGCCATTGGGTGTT GGTATTAGTGGACACCCATTATATAATAGAC AGGATGATACTGAAAACTCACCATTTTCATCA ACCACCAATAAGGACAGTAGGGATAATGTGT CTGTGGATTATAAACAGACACAGTTGTGCATT ATAGGCTGTGTTCCCGCCATTGGGGAGCACT GGGGTAAGGGAAAGGCATGCAAGCCCAATA ATGTATCTACGGGGGACTGTCCTCCTTTGGA ACTAGTAAACACCCCTATTGAGGATGGTGAT ATGATTGATACTGGCTATGGAGCTATGGACT TTGGTGCATTGCAGGAAACCAAAAGTGAGGT GCCTTTAGATATTTGTCAATCCATTTGTAAAT ATCCTGATTATTTGCAAATGTCTGCAGATGTG TATGGGGACAGTATGTTCTTCTGTTTACGTAG GGAACAACTGTTTGCAAGACATTTTTGGAATC GTGGTGGTATGGTGGGTGACGCCATTCCTGC CCAATTGTATATTAAGGGCACAGATATACGT GCAAACCCCGGTAGTTCTGTATACTGCCCCTC TCCCAGCGGTTCCATGGTAACCTCTGATTCCC AGTTATTTAATAAGCCTTATTGGCTACATAAG GCCCAGGGCCACAACAATGGTATATGTTGGC ATAATCAATTATTTCTTACTGTTGTGGACACT ACCCGTAGTACCAACTTTACATTATCTACCTCT ATAGAGTCTTCCATACCTTCTACATATGATCC TTCTAAGTTTAAGGAATATACCAGGCACGTG GAGGAGTATGATTTACAATTTATATTTCAACT GTGTACTGTCACATTAACAACTGATGTTATGT CTTATATTCACACTATGAATTCCTCTATATTGG ACAATTGGAATTTTGCTGTAGCTCCTCCACCA TCTGCCAGTTTGGTAGACACTTACAGATACCT ACAGTCTGCAGCCATTACATGTCAAAAGGAT GCTCCAGCACCTGAAAAGAAAGATCCATATG ACGGTCTAAAGTTTTGGAATGTTGACTTAAG GGAAAAGTTTAGTTTGGAACTTGATCAATTCC CTTTGGGACGTAAATTTTTGTTGCAGGCCAG GGTCCGCAGGCGCCCTACTATAGGTCCCCGA AAGCGGCCTGCTGCATCCACTTCCTCGTCCTC AGCTACTAAACACAAACGTAAACGTGTGTCT AAATAA ATGCGAGGCCCAAAGCCAACTCTGCAGGAAA TTGTGCTGGACCTGTGCCCCTATAATGAGATT CAGCCCGTGGACCTGGTGTGTCACGAACAGC TGGGCGAGTCTGAAGACGAGATCGATGAGC CCGACCACGCAGTGAACCACCAGCATCAGCT GCTGGCCCGGAGAGATGAACCTCAGCGACAT ACCATTCAGTGCAGCTGCTGTAAGTGTAACA ATACACTGCAGCTGGTGGTCGAGGCTAGCAG GGATACTCTGCGCCAGCTGCAGCAGCTGTTC ATGGACTCCCTGGGGTTTGTCTGCCCCTGGT GTGCCACCGCTAATCAG ATGGCTATGTGGCGAAGCTCCGATTCTATGG TCTATCTGCCCCCCCCCTCCGTGGCTAAAGTC GTGAACACCGATGATTATGTGACACGGACAG GAATCTACTATTACGCAGGCAGCTCCAGACT GCTGACTGTCGGCCACCCATATTTCAAAGTG GGGATGAATGGCGGGAGAAAACAGGACATC CCCAAGGTGAGCGCTTATCAGTACCGAGTCT TCCGGGTGACCCTGCCAGACCCCAACAAGTT TAGTATTCCTGATGCATCACTGTACAATCCAG AGACACAGAGGCTGGTCTGGGCATGCGTGG GAGTCGAAGTGGGACGAGGACAGCCACTGG GAGTGGGAATCAGTGGACACCCTCTGTATAA CCGACAGGACGATACAGAGAATTCTCCCTTCT CTAGTACCACAAACAAAGACAGCCGGGATAA TGTCTCCGTGGACTACAAGCAGACTCAGCTG TGCATCATTGGGTGTGTGCCTGCCATTGGAG AACATTGGGGAAAGGGCAAAGCTTGCAAGC CAAACAATGTCAGCACAGGCGATTGTCCCCC TCTGGAGCTGGTGAACACACCTATCGAAGAC GGGGATATGATTGACACTGGGTATGGAGCTA TGGATTTTGGAGCACTGCAGGAGACCAAATC CGAAGTCCCACTGGACATCTGCCAGTCTATTT GTAAGTATCCCGATTACCTGCAGATGTCAGCT GACGTGTACGGGGATAGCATGTTCTTTTGTCT GCGGAGAGAGCAGCTGTTCGCAAGACACTTT TGGAACAGGGGAGGAATGGTGGGCGACGCA ATCCCAGCACAGCTGTATATCAAGGGAACTG ATATTAGGGCCAATCCTGGCTCAAGCGTCTAC TGCCCTTCACCAAGCGGCTCCATGGTGACCTC TGACAGTCAGCTGTTTAACAAACCCTACTGGC TGCACAAGGCCCAGGGCCATAACAATGGGAT TTGTTGGCATAACCAGCTGTTCCTGACAGTG GTCGATACTACCAGAAGCACTAATTTTACCCT GTCAACAAGCATCGAGTCCTCTATTCCATCTA CCTATGACCCCAGTAAGTTCAAAGAATATACA AGGCACGTGGAGGAATACGATCTGCAGTTCA TCTTTCAGCTGTGCACCGTCACACTGACAACT GACGTGATGTCCTACATCCATACCATGAACA GTTCAATCCTGGATAACTGGAACTTCGCTGTC GCACCACCCCCTTCCGCCTCTCTGGTGGACAC TTATCGCTACCTGCAGTCCGCCGCTATTACCT GTCAGAAAGATGCCCCCGCTCCTGAGAAGAA AGACCCTTACGATGGCCTGAAATTCTGGAAC GTGGACCTGCGGGAGAAGTTTTCTCTGGAAC TGGATCAGTTCCCACTGGGACGCAAATTTCT GCTGCAGGCACGAGTGAGGCGACGACCAAC CATCGGACCACGAAAGAGACCTGCAGCAAGC ACTAGCTCCTCTAGTGCTACCAAGCATAAAAG GAAGCGCGTGAGCAAG MRGPKPTLQEIVLDLCPYNEIQPVDL VCHEQLGESEDEIDEPDHAVNHQH QLLARRDEPQRHTIQCSCCKCNNTL QLVVEASRDTLRQLQQLFMDSLGFV CPWCATANQ HPV39 L1 Page 31 of 48 MAMWRSSDSMVYLPPPSVAKVVN TDDYVTRTGIYYYAGSSRLLTVGHPY FKVGMNGGRKQDIPKVSAYQYRVF RVTLPDPNKFSIPDASLYNPETQRLV WACVGVEVGRGQPLGVGISGHPLY NRQDDTENSPFSSTTNKDSRDNVSV DYKQTQLCIIGCVPAIGEHWGKGKA CKPNNVSTGDCPPLELVNTPIEDGD MIDTGYGAMDFGALQETKSEVPLDI CQSICKYPDYLQMSADVYGDSMFFC LRREQLFARHFWNRGGMVGDAIPA QLYIKGTDIRANPGSSVYCPSPSGSM VTSDSQLFNKPYWLHKAQGHNNGI CWHNQLFLTVVDTTRSTNFTLSTSIE SSIPSTYDPSKFKEYTRHVEEYDLQFIF QLCTVTLTTDVMSYIHTMNSSILDN WNFAVAPPPSASLVDTYRYLQSAAIT CQKDAPAPEKKDPYDGLKFWNVDL REKFSLELDQFPLGRKFLLQARVRRR PTIGPRKRPAASTSSSSATKHKRKRVS K % Nucleotide Change 23.5% 26.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV39 L2 ATGGTTTCCCACCGTGCTGCCAGGCGTAAGC GTGCATCTGCAACTGACCTATATAGAACCTGT AAACAATCGGGTACCTGTCCACCAGACGTTG TTGATAAAGTTGAGGGTACTACACTTGCTGA CAAAATTTTACAGTGGACTAGTTTAGGTATAT TTTTGGGTGGGTTAGGCATAGGCACAGGTAC TGGTACTGGGGGACGCACAGGATATATACCC CTGGGGGGTAGGCCTAATACTGTTGTAGATG TGTCTCCTGCACGTCCACCTGTAGTTATTGAA CCTGTTGGTCCTTCTGAGCCATCTATTGTGCA ATTGGTGGAGGACTCAAGTGTTATAACCTCT GGAACACCAGTACCAACATTTACAGGCACCT CTGGATTTGAAATTACTTCTTCTTCTACTACTA CGCCTGCGGTATTGGATATTACACCCTCCTCT GGGTCTGTACAAATAACCTCTACTAGTTATAC TAACCCTGCCTTTACGGATCCTTCCTTAATTG AGGTTCCCCAAACAGGTGAAACCTCGGGTAA TATATTTGTCAGTACCCCTACATCAGGTACAC ATGGCTATGAGGAAATACCTATGGAAGTGTT TGCCACACATGGCACAGGTACCGAACCTATT AGCAGCACACCTACACCTGGAATCAGTCGTG TGGCAGGACCACGTTTATATAGTAGAGCACA TCAGCAGGTTCGTGTTAGTAATTTTGATTTTG TAACTCACCCTTCATCATTTGTAACATTTGATA ATCCTGCTTTTGAGCCTGTTGATACTACATTA ACATATGAAGCTGCTGACATAGCTCCAGATC CGGATTTTCTGGACATTGTTCGTTTACATAGG CCTGCCTTAACCTCGCGTAAAGGAACAGTAA GGTTTAGTAGGCTTGGCAAAAAGGCTACCAT GGTTACCCGGCGTGGCACACAAATTGGAGCG CAAGTACATTATTACCATGACATTAGTAGTAT TGCTCCTGCTGAAAGCATTGAATTACAGCCCC TAGTTCACGCTGAGCCCTCTGATGCTTCAGAT GCATTATTTGATATATATGCTGATGTGGACAA TAACACATATTTAGATACTGCATTTAATAATA CAAGGGATTCGGGCACTACATATAACACAGG CTCACTACCTTCTGTGGCTTCTTCAGCATCTAC TAAATATGCCAATACAACTATTCCTTTTAGTA CCTCATGGAATATGCCTGTAAATACTGGTCCT GATATTGCTTTACCAAGTACTACTCCACAGTT GCCATTGGTGCCTTCTGGACCAATAGACACA ACATATGCAATAACCATTCAGGGTTCCAATTA TTATTTGTTGCCATTATTGTATTTTTTCCTAAA AAAACGTAAACGTATTCCCTATTTTTTTTCAG ATGGCTATGTGGCGGTCTAG ATGGTCTCCCACCGGGCAGCAAGAAGGAAAC GGGCATCAGCAACCGACCTGTATCGCACCTG TAAGCAGAGCGGAACCTGTCCCCCCGACGTG GTCGATAAGGTGGAGGGGACCACACTGGCT GACAAAATTCTGCAGTGGACCTCTCTGGGAA TCTTCCTGGGAGGACTGGGAATTGGAACCGG AACAGGCACTGGAGGCCGGACTGGCTATATC CCACTGGGGGGAAGACCCAACACCGTGGTC GACGTGTCCCCAGCAAGACCACCTGTGGTCA TCGAGCCAGTCGGACCATCAGAACCTAGCAT CGTGCAGCTGGTCGAGGATAGCTCCGTGATC ACTTCAGGGACCCCAGTCCCCACCTTCACAGG CACTAGCGGGTTTGAAATCACATCTAGTTCAA CTACCACACCAGCTGTGCTGGACATCACCCCC AGCTCCGGCAGCGTCCAGATTACCAGCACAT CCTACACAAACCCTGCATTCACTGATCCATCC CTGATTGAGGTGCCTCAGACAGGGGAAACTT CTGGAAATATCTTTGTCAGCACTCCTACCTCC GGAACACACGGCTATGAGGAAATCCCAATGG AGGTGTTCGCCACCCATGGGACAGGAACTGA ACCAATCTCTAGTACCCCTACACCAGGAATTT CAAGGGTGGCAGGACCACGACTGTACAGCC GAGCACACCAGCAGGTGCGCGTCTCCAACTT CGATTTTGTGACTCATCCCTCAAGCTTCGTCA CCTTTGACAATCCCGCCTTTGAGCCTGTGGAT ACTACCCTGACATACGAAGCCGCTGACATCG CACCCGACCCTGATTTCCTGGATATTGTGCGA CTGCACCGACCTGCACTGACCTCTCGAAAGG GCACCGTGCGGTTCAGCAGGCTGGGGAAGA AAGCCACCATGGTGACACGGAGAGGCACCC AGATTGGGGCTCAGGTCCACTACTATCATGA CATCTCCTCTATTGCACCCGCCGAGAGCATCG AACTGCAGCCACTGGTGCATGCAGAGCCTTC CGACGCTTCTGATGCACTGTTCGATATCTACG CTGACGTGGATAACAATACTTATCTGGACACC GCCTTCAACAATACCCGCGATAGTGGGACAA CTTACAACACAGGAAGTCTGCCTTCAGTGGC CAGTTCAGCTAGCACCAAGTATGCTAATACCA CAATTCCATTTTCTACAAGTTGGAACATGCCC GTGAATACTGGCCCTGACATCGCACTGCCATC TACTACCCCACAGCTGCCCCTGGTGCCTAGTG GACCAATTGATACAACTTACGCCATCACCATT CAGGGCAGCAATTACTATCTGCTGCCCCTGCT GTATTTCTTTCTGAAGAAAAGGAAACGCATCC CTTACTTCTTTTCCGACGGCTATGTGGCCGTC MVSHRAARRKRASATDLYRTCKQSG TCPPDVVDKVEGTTLADKILQWTSL GIFLGGLGIGTGTGTGGRTGYIPLGG RPNTVVDVSPARPPVVIEPVGPSEPS IVQLVEDSSVITSGTPVPTFTGTSGFEI TSSSTTTPAVLDITPSSGSVQITSTSYT NPAFTDPSLIEVPQTGETSGNIFVSTP TSGTHGYEEIPMEVFATHGTGTEPIS STPTPGISRVAGPRLYSRAHQQVRVS NFDFVTHPSSFVTFDNPAFEPVDTTL TYEAADIAPDPDFLDIVRLHRPALTSR KGTVRFSRLGKKATMVTRRGTQIGA QVHYYHDISSIAPAESIELQPLVHAEP SDASDALFDIYADVDNNTYLDTAFN NTRDSGTTYNTGSLPSVASSASTKYA NTTIPFSTSWNMPVNTGPDIALPSTT PQLPLVPSGPIDTTYAITIQGSNYYLLP LLYFFLKKRKRIPYFFSDGYVAV Page 32 of 48 % Nucleotide Change 27.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV45 E1 ATGGCGGATCCAGAAGGTACCGACGGGGAG GGAACGGGGTGTAATGGCTGGTTCTTTGTAG AAACAATTGTAGAGAAAAAAACAGGGGATG TAATATCAGATGATGAGGATGAAACTGCAAC AGATACAGGGTCGGATATGGTAGATTTTATT GACACACAATTATCCATTTGTGAACAGGCAG AGCAAGAGACAGCACAGGCATTGTTCCATGC GCAGGAAGTTCAGAATGATGCACAGGTGTTG CATCTTTTAAAACGAAAGTTTGCAGGAGGCA GCAAGGAAAACAGTCCATTAGGGGAGCAGC TAAGTGTGGATACGGATCTAAGTCCACGGTT ACAAGAAATTTCATTAAATAGTGGGCACAAA AAAGCAAAACGACGGTTGTTTACAATATCAG ATAGTGGCTATGGCTGTTCTGAAGTGGAAGC TGCAGAGACTCAGGTAACTGTAAACACTAAT GCGGAAAATGGCGGCAGTGTACATAGTACAC AAAGTAGTGGTGGGGATAGTAGTGACAATG CAGAAAATGTAGATCCGCATTGCAGTATTAC AGAACTAAAGGAGCTATTACAAGCAAGTAAC AAAAAGGCTGCAATGCTGGCAGTATTTAAAG ACATATATGGGCTGTCATTTACGGATTTGGTT AGAAATTTTAAAAGTGATAAAACAACATGTA CAGATTGGGTAATGGCTATATTTGGAGTTAA TCCAACGGTAGCAGAAGGCTTTAAAACATTA ATTAAACCAGCAACGTTATACGCCCATATCCA ATGTTTAGATTGTAAATGGGGAGTATTAATA TTAGCTTTATTAAGATATAAATGTGGCAAAAA TAGACTAACTGTTGCAAAAGGCTTAAGCACA TTGTTGCACGTACCTGAAACATGTATGTTAAT TGAACCACCAAAATTGCGAAGTAGTGTTGCA GCATTATACTGGTATAGAACAGGTATATCCA ATATTAGTGAAGTAAGTGGAGACACACCTGA GTGGATACAAAGACTGACAATTATTCAACAT GGTATTGACGATAGTAATTTTGATTTGTCAGA CATGGTGCAATGGGCATTTGATAATGACCTT ACAGATGAAAGTGATATGGCATTTCAATATG CCCAATTAGCAGACTGCAACAGTAATGCAGC TGCATTTTTAAAAAGTAACTGCCAAGCCAAAT ATTTAAAAGATTGTGCTGTAATGTGTAGACAT TATAAAAGAGCACAAAAACGCCAAATGAATA TGTCTCAATGGATTAAATATAGATGTTCCAAA ATAGATGAAGGTGGGGATTGGAGACCCATA GTACAATTCCTAAGATATCAGGGAGTAGAAT TTATTAGCTTTTTAAGGGCACTAAAGGAATTT CTTAAAGGAACACCAAAAAAAAATTGTATAC TGTTATATGGACCTGCAAATACAGGAAAATC GTATTTTGGAATGAGTTTTATACATTTCCTAC AAGGTGCAATAATATCATTTGTAAATTCAAAC AGCCATTTTTGGTTAGAACCGTTAGCAGATAC TAAGGTAGCCATGTTGGATGATGCCACACAC ACGTGTTGGACATATTTTGATAATTATATGAG AAATGCATTAGATGGTAATCCTATAAGTATA GACAGAAAGCATAAACCATTATTACAGCTAA AATGTCCTCCAATCCTATTAACATCCAATATT GATCCAGCAAAAGATAATAAATGGCCATATT TAGAAAGTAGGGTGACGGTATTTACATTTCC ACATGCATTTCCATTTGATAAAAATGGTAATC CAGTATATGAAATAAATGATAAAAATTGGAA ATGTTTTTTTGAAAGGACATGGTCCAGATTAG ATTTGCACGAGGACGATGAAGATGCAGACAC CGAAGGAATCCCTTTCGGAACGTTTAAGTGC GTTACAGGACAAAATACTAGACCACTATGA ATGGCAGATCCTGAGGGGACTGACGGGGAG GGGACTGGGTGTAATGGGTGGTTTTTCGTGG AGACTATCGTGGAGAAGAAGACTGGGGATG TGATCTCCGACGATGAGGATGAAACTGCCAC CGACACAGGCTCCGACATGGTCGATTTCATC GACACTCAGCTGTCTATTTGCGAGCAGGCTG AGCAGGAAACCGCCCAGGCTCTGTTCCACGC CCAGGAAGTGCAGAACGACGCTCAGGTCCTG CATCTGCTGAAGCGCAAATTTGCCGGCGGGT CTAAGGAGAATAGTCCACTGGGAGAACAGCT GAGTGTGGACACAGATCTGTCACCCCGGCTG CAGGAGATCAGCCTGAACTCCGGCCACAAGA AAGCCAAACGGAGACTGTTCACCATTTCCGA TTCTGGATACGGCTGCAGCGAGGTGGAAGCC GCTGAGACACAAGTGACTGTCAACACCAATG CAGAAAATGGAGGCTCTGTGCACAGTACACA GAGCTCCGGGGGAGATTCTAGTGACAACGCC GAAAATGTCGACCCTCATTGTAGTATCACTGA GCTGAAGGAACTGCTGCAGGCTTCAAACAAG AAAGCAGCCATGCTGGCAGTGTTTAAAGATA TCTACGGCCTGTCATTCACCGACCTGGTCCGG AACTTTAAGAGCGATAAAACCACATGTACAG ACTGGGTCATGGCCATCTTCGGCGTGAATCC CACTGTCGCTGAGGGGTTTAAGACTCTGATC AAACCTGCTACCCTGTACGCACACATTCAGTG CCTGGATTGTAAGTGGGGCGTGCTGATCCTG GCTCTGCTGAGGTATAAGTGCGGAAAAAATC GCCTGACAGTGGCAAAGGGCCTGTCCACTCT GCTGCATGTCCCCGAGACCTGTATGCTGATC GAACCCCCTAAACTGAGATCAAGCGTGGCTG CACTGTACTGGTATAGGACAGGGATCTCAAA CATTAGCGAGGTCTCCGGAGACACCCCTGAA TGGATCCAGAGACTGACAATCATTCAGCACG GCATTGACGATTCAAACTTCGATCTGAGCGA CATGGTGCAGTGGGCATTTGACAATGATCTG ACCGATGAGTCTGACATGGCCTTCCAGTACG CACAGCTGGCCGATTGCAACAGCAATGCCGC TGCATTTCTGAAGTCCAACTGTCAGGCAAAGT ACCTGAAAGACTGCGCCGTGATGTGTAGGCA TTATAAGCGCGCCCAGAAACGACAGATGAAT ATGTCTCAGTGGATCAAGTACAGATGCAGTA AAATTGATGAGGGCGGGGACTGGCGACCAA TCGTGCAGTTCCTGCGGTATCAGGGCGTCGA GTTCATTTCTTTTCTGAGGGCCCTGAAGGAAT TTCTGAAAGGGACCCCTAAGAAAAACTGTAT CCTGCTGTACGGCCCAGCCAATACCGGGAAG TCCTATTTCGGAATGTCTTTCATTCACTTTCTG CAGGGGGCTATCATCAGTTTCGTGAACAGTA ACTCACATTTCTGGCTGGAGCCCCTGGCCGAT ACTAAAGTCGCTATGCTGGACGATGCAACTC ACACCTGCTGGACCTACTTTGACAACTATATG CGCAATGCCCTGGATGGAAATCCAATCAGCA TTGACCGAAAGCATAAACCCCTGCTGCAGCT GAAGTGTCCACCCATCCTGCTGACCAGCAAC ATTGATCCCGCTAAGGACAACAAGTGGCCTT ACCTGGAGTCCCGCGTGACAGTCTTCACTTTT CCTCACGCATTCCCATTTGATAAGAACGGCAA TCCCGTGTATGAGATCAACGACAAGAATTGG AAATGCTTCTTTGAACGGACCTGGAGCAGAC TGGACCTGCATGAGGACGATGAAGACGCCG ATACAGAAGGGATTCCTTTCGGAACTTTTAAG TGTGTGACCGGGCAGAACACAAGGCCACTG MADPEGTDGEGTGCNGWFFVETIV EKKTGDVISDDEDETATDTGSDMVD FIDTQLSICEQAEQETAQALFHAQEV QNDAQVLHLLKRKFAGGSKENSPLG EQLSVDTDLSPRLQEISLNSGHKKAK RRLFTISDSGYGCSEVEAAETQVTVN TNAENGGSVHSTQSSGGDSSDNAE NVDPHCSITELKELLQASNKKAAMLA VFKDIYGLSFTDLVRNFKSDKTTCTD WVMAIFGVNPTVAEGFKTLIKPATLY AHIQCLDCKWGVLILALLRYKCGKNR LTVAKGLSTLLHVPETCMLIEPPKLRS SVAALYWYRTGISNISEVSGDTPEWI QRLTIIQHGIDDSNFDLSDMVQWAF DNDLTDESDMAFQYAQLADCNSNA AAFLKSNCQAKYLKDCAVMCRHYKR AQKRQMNMSQWIKYRCSKIDEGG DWRPIVQFLRYQGVEFISFLRALKEFL KGTPKKNCILLYGPANTGKSYFGMSF IHFLQGAIISFVNSNSHFWLEPLADTK VAMLDDATHTCWTYFDNYMRNAL DGNPISIDRKHKPLLQLKCPPILLTSNI DPAKDNKWPYLESRVTVFTFPHAFP FDKNGNPVYEINDKNWKCFFERTW SRLDLHEDDEDADTEGIPFGTFKCVT GQNTRPL Page 33 of 48 % Nucleotide Change 26.4% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV45 E2 ATGAAGATGCAGACACCGAAGGAATCCCTTT CGGAACGTTTAAGTGCGTTACAGGACAAAAT ACTAGACCACTATGAAAATGACAGTAAAGAC ATAAACAGCCAAATAAGTTATTGGCAACTTAT ACGTTTGGAAAATGCAATACTATTTACAGCAA GGGAACATGGTATTACCAAACTAAACCACCA GGTGGTGCCTCCTATTAACATTTCAAAAAGCA AAGCACATAAAGCTATTGAACTGCAAATGGC CTTAAAGGGCCTTGCACAAAGCAAGTATAAC AATGAGGAATGGACACTGCAAGATACATGCG AGGAACTATGGAATACAGAACCGTCGCAGTG TTTTAAAAAAGGCGGTAAAACCGTGCACGTA TACTTTGATGGCAACAAGGACAACTGTATGA ACTATGTAGTATGGGACAGTATATATTATATA ACTGAGACAGGGATATGGGACAAAACAGCA GCATGTGTTAGCTATTGGGGTGTATATTATAT AAAAGATGGAGATACCACATATTATGTACAA TTTAAAAGCGAATGTGAGAAATATGGAAATA GTAATACGTGGGAAGTACAATATGGGGGCA ATGTAATTGATTGTAATGACTCTATGTGCAGT ACCAGTGACGACACGGTATCCGCTACTCAGA TTGTTAGACAGCTACAACACGCCTCCACGTCG ACCCCCAAAACCGCATCCGTGGGCACCCCAA AACCCCACATCCAGACGCCGGCTACTAAGCG ACCTAGACAGTGTGGACTCACAGAGCAGCAC CACGGACGTGTCAACACCCACGTGCACAACC CGCTCCTGTGTTCAAGTACAAGTAACAACAA AAGAAGGAAAGTGTGTAGTGGTAACACTAC GCCTATAATACACTTAAAAGGTGACAAAAAC AGTTTGAAATGTTTAAGATATAGGCTACGCA AATATGCAGACCATTACTCAGAAATATCCTCC ACCTGGCATTGGACAGGTTGTAATAAAAACA CTGGTATATTAACTGTAACATATAATAGTGAG GTACAAAGAAATACCTTTTTGGATGTAGTTAC TATTCCTAACAGTGTACAAATCTCGGTGGGAT ACATGACTATATGA ATGACTCTATGTGCAGTACCAGTGACGACAC GGTATCCGCTACTCAGATTGTTAGACAGCTAC AACACGCCTCCACGTCGACCCCCAAAACCGC ATCCGTGGGCACCCCAAAACCCCACATCCAG ACGCCGGCTACTAAGCGACCTAGACAGTGTG GACTCACAGAGCAGCACCACGGACGTGTCAA CACCCACGTGCACAACCCGCTCCTGTGTTCAA GTACAAGTAACAACAAAAGAAGGAAAGTGT GTAGTGGTAACACTACGCCTATAA ATGCTATCTTTAGTGTTTTTATTGTGCTTTTCT GTGTGCCTTTATGTGTGCTGCAATGTCCCGCT TGTGCAGTCTGTCTATGTGTGTGCTTTTGCTT GGTTGTTGGTGTTTCTTTTTATAGTTGTTATTA CATCCCCATTAACAGCATTTGCTGTATACATT TGTTGCTATTTACTACCTATGTTTGTATTACAT ATGCATGCTTTACACACCATACAATAA ATGGCGCGCTTTGACGATCCAAAGCAACGAC CCTACAAGCTACCAGATTTGTGCACAGAATTG AATACATCACTACAAGACGTATCTATTGCCTG TGTATATTGCAAAGCAACATTGGAACGCACA GAGGTATATCAATTTGCTTTTAAAGATTTATG TATAGTGTATAGAGACTGTATAGCATATGCT GCATGCCATAAATGTATAGACTTTTATTCCAG AATTAGAGAATTAAGATATTATTCAAACTCTG TATATGGAGAGACACTGGAAAAAATAACTAA TACAGAGTTGTATAATTTGTTAATAAGGTGCC TGCGGTGCCAGAAACCATTGAACCCAGCAGA AAAACGTAGACACCTTAAGGACAAACGAAGA TTTCACAGCATAGCTGGACAGTACCGAGGGC AGTGTAATACATGTTGTGACCAGGCACGGCA AGAAAGACTTCGCAGACGTAGGGAAACACA AGTATAG ATGAAGATGCAGACTCCCAAGGAAAGCCTGA GCGAGAGACTGAGCGCACTGCAGGATAAGA TTCTGGACCATTACGAAAACGACTCCAAGGA CATCAATAGCCAGATTTCCTACTGGCAGCTGA TCAGGCTGGAGAACGCTATTCTGTTCACTGC ACGCGAACACGGAATCACCAAGCTGAATCAT CAGGTGGTCCCCCCTATCAACATTTCAAAGAG CAAAGCACACAAAGCCATTGAGCTGCAGATG GCCCTGAAGGGCCTGGCTCAGAGCAAATATA ACAATGAGGAATGGACCCTGCAGGATACATG CGAGGAACTGTGGAATACCGAACCATCCCAG TGTTTCAAGAAAGGCGGGAAGACAGTGCAT GTCTACTTTGACGGCAACAAAGATAATTGCAT GAACTATGTGGTCTGGGACTCTATCTACTATA TTACAGAGACTGGGATCTGGGATAAGACAGC CGCTTGTGTGAGTTACTGGGGCGTCTACTAT ATCAAGGACGGGGATACCACATACTATGTGC AGTTTAAGTCAGAGTGCGAAAAATACGGGAA CAGCAATACCTGGGAAGTGCAGTATGGAGG CAATGTCATCGACTGCAACGATAGTATGTGTT CCACTTCTGACGATACCGTGTCAGCTACACAG ATTGTGCGACAGCTGCAGCACGCAAGTACCT CAACACCCAAGACAGCCTCTGTGGGCACTCC AAAACCCCATATCCAGACACCTGCCACTAAG AGGCCACGCCAGTGTGGACTGACAGAGCAG CACCATGGCAGGGTGAATACTCACGTCCATA ACCCCCTGCTGTGCAGCTCCACCTCCAACAAT AAGCGGAGAAAAGTGTGTTCTGGGAATACTA CCCCTATCATTCACCTGAAGGGAGACAAAAA CAGCCTGAAGTGTCTGCGATACCGGCTGAGA AAATACGCCGATCACTATTCCGAGATCTCTAG TACTTGGCATTGGACCGGGTGCAACAAGAAT ACTGGAATTCTGACTGTGACCTACAATAGCG AAGTGCAGCGGAACACCTTCCTGGACGTGGT CACAATCCCTAACTCTGTGCAGATTAGTGTCG GCTATATGACCATC ATGACCCTGTGTGCCGTCCCCGTGACCACCC GCTACCCACTGCTGCGACTGCTGGATAGTTA CAATACCCCACCCCGAAGACCCCCTAAGCCAC ACCCTTGGGCACCACAGAACCCAACTTCACG GAGAAGGCTGCTGAGCGACCTGGATTCTGTG GACAGTCAGAGCTCCACCACAGATGTGTCCA CCCCCACATGCACTACCCGCAGTTGTGTCCAG GTGCAGGTCACAACTAAGGAGGGCAAATGC GTGGTCGTGACACTGCGACTG ATGCTGAGTCTGGTCTTTCTGCTGTGTTTTTCC GTCTGTCTGTATGTGTGTTGTAATGTCCCCCT GGTGCAGTCCGTCTATGTCTGTGCCTTCGCTT GGCTGCTGGTGTTCCTGTTTATCGTGGTCATT ACCAGCCCCCTGACAGCATTCGCCGTGTACAT CTGCTGTTATCTGCTGCCTATGTTTGTCCTGC ACATGCATGCTCTGCACACCATTCAG ATGGCCCGATTTGATGACCCTAAACAGCGCC CCTATAAACTGCCCGATCTGTGTACCGAACTG AATACTTCTCTGCAGGATGTGAGCATCGCAT GCGTGTACTGTAAGGCCACTCTGGAGCGAAC CGAAGTCTATCAGTTCGCTTTTAAAGACCTGT GCATCGTGTACCGCGATTGTATTGCATATGCC GCTTGCCACAAGTGTATCGACTTCTACTCTCG CATTCGAGAGCTGAGATACTATAGCAACTCC GTCTACGGCGAGACCCTGGAAAAAATCACCA ACACAGAGCTGTATAATCTGCTGATTCGGTG CCTGAGATGTCAGAAGCCCCTGAACCCTGCC GAAAAACGGAGACACCTGAAGGACAAAAGG CGCTTTCATAGCATTGCCGGCCAGTATCGGG GGCAGTGCAATACATGCTGTGATCAGGCTAG GCAGGAGCGCCTGCGACGGAGAAGGGAAAC TCAGGTG MKMQTPKESLSERLSALQDKILDHYE NDSKDINSQISYWQLIRLENAILFTAR EHGITKLNHQVVPPINISKSKAHKAIE LQMALKGLAQSKYNNEEWTLQDTC EELWNTEPSQCFKKGGKTVHVYFDG NKDNCMNYVVWDSIYYITETGIWD KTAACVSYWGVYYIKDGDTTYYVQF KSECEKYGNSNTWEVQYGGNVIDC NDSMCSTSDDTVSATQIVRQLQHAS TSTPKTASVGTPKPHIQTPATKRPRQ CGLTEQHHGRVNTHVHNPLLCSSTS NNKRRKVCSGNTTPIIHLKGDKNSLK CLRYRLRKYADHYSEISSTWHWTGC NKNTGILTVTYNSEVQRNTFLDVVTI PNSVQISVGYMTI HPV45 E4 HPV45 E5 HPV45 E6 Page 34 of 48 % Nucleotide Change 25.4% MTLCAVPVTTRYPLLRLLDSYNTPPR RPPKPHPWAPQNPTSRRRLLSDLDS VDSQSSTTDVSTPTCTTRSCVQVQV TTKEGKCVVVTLRL 28.5% MLSLVFLLCFSVCLYVCCNVPLVQSV YVCAFAWLLVFLFIVVITSPLTAFAVYI CCYLLPMFVLHMHALHTIQ 24.2% MARFDDPKQRPYKLPDLCTELNTSL QDVSIACVYCKATLERTEVYQFAFKD LCIVYRDCIAYAACHKCIDFYSRIRELR YYSNSVYGETLEKITNTELYNLLIRCLR CQKPLNPAEKRRHLKDKRRFHSIAG QYRGQCNTCCDQARQERLRRRRET QV 25.7% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV45 E7 ATGCATGGACCCCGGGAAACACTGCAAGAAA TTGTATTGCATTTGGAACCTCAGAATGAATTA GATCCTGTTGACCTGTTGTGTTACGAGCAATT AAGCGAGTCAGAGGAGGAAAACGATGAAGC AGATGGAGTTAGTCATGCACAACTACCAGCC CGACGAGCCGAACCACAGCGTCACAAAATTT TGTGTGTATGTTGTAAGTGTGACGGCAGAAT TGAGCTTACAGTAGAGAGCTCGGCAGAGGA CCTTAGAACACTACAGCAGCTGTTTTTGAGCA CCTTGTCCTTTGTGTGTCCGTGGTGTGCACTA ACCAATAA ATGGCTTTGTGGCGGCCTAGTGACAGTACGG TATATCTTCCACCACCTTCTGTGGCCAGAGTT GTCAGCACTGATGATTATGTGTCTCGCACAA GCATATTTTATCATGCAGGCAGTTCCCGATTA TTAACTGTAGGCAATCCATATTTTAGGGTTGT ACCTAATGGTGCAGGTAATAAACAGGCTGTT CCTAAGGTATCCGCATATCAGTATAGGGTGT TTAGAGTAGCTTTACCCGATCCTAATAAATTT GGATTACCTGATTCTACTATATATAATCCTGA AACACAACGTTTGGTTTGGGCATGTGTAGGT ATGGAAATTGGTCGTGGGCAGCCTTTAGGTA TTGGCCTAAGTGGCCATCCATTTTATAATAAA TTGGATGATACAGAAAGTGCTCATGCAGCTA CAGCTGTTATTACGCAGGATGTTAGGGATAA TGTGTCAGTTGATTATAAGCAAACACAGCTGT GTATTTTAGGTTGTGTACCTGCTATTGGTGAG CACTGGGCCAAGGGCACACTTTGTAAACCTG CACAATTGCAACCTGGTGACTGTCCTCCTTTG GAACTTAAAAACACCATTATTGAGGATGGTG ATATGGTGGATACAGGTTATGGGGCAATGGA TTTTAGTACATTGCAGGATACAAAGTGCGAG GTTCCATTAGACATTTGTCAATCCATCTGTAA ATATCCAGATTATTTGCAAATGTCTGCTGATC CCTATGGGGATTCTATGTTTTTTTGCCTACGC CGTGAACAACTGTTTGCAAGACATTTTTGGAA TAGGGCAGGTGTTATGGGTGACACAGTACCT ACGGACCTATATATTAAAGGCACTAGCGCTA ATATGCGTGAAACCCCTGGCAGTTGTGTGTA TTCCCCTTCTCCCAGTGGCTCTATTATTACTTC TGATTCTCAATTATTTAATAAGCCATATTGGT TACATAAGGCCCAGGGCCATAACAATGGTAT TTGTTGGCATAATCAGTTGTTTGTTACTGTAG TGGACACTACCCGCAGTACTAATTTAACATTA TGTGCCTCTACACAAAATCCTGTGCCAAGTAC ATATGACCCTACTAAGTTTAAGCAGTATAGTA GACATGTGGAGGAATATGATTTACAGTTTAT TTTTCAGTTGTGCACTATTACTTTAACTGCAG AGGTTATGTCATATATCCATAGTATGAATAGT AGTATATTAGAAAATTGGAATTTTGGTGTCCC TCCACCACCTACTACAAGTTTGGTGGATACAT ATCGTTTTGTGCAATCAGTTGCTGTTACCTGT CAAAAGGATACTACACCTCCAGAAAAGCAGG ATCCATATGATAAATTAAAGTTTTGGACTGTT GACCTAAAGGAAAAATTTTCCTCCGATTTGGA TCAATATCCCCTTGGTCGAAAGTTTTTAGTTC AGGCTGGGTTACGTCGTAGGCCTACCATAGG ACCTCGTAAGCGTCCTGCTGCTTCCACGTCTA CTGCATCTACTGCATCTAGGCCTGCCAAACGT GTACGTATACGTAGTAAGAAATAA ATGCATGGACCAAGAGAAACCCTGCAGGAA ATCGTGCTGCATCTGGAGCCTCAGAATGAAC TGGACCCTGTGGACCTGCTGTGCTATGAACA GCTGTCTGAGAGTGAGGAAGAGAACGACGA GGCAGATGGCGTGTCTCACGCACAGCTGCCA GCACGAAGAGCTGAACCTCAGAGGCATAAG ATCCTGTGCGTGTGCTGTAAATGTGACGGGC GCATTGAACTGACTGTCGAGAGCTCCGCTGA AGATCTGCGAACCCTGCAGCAGCTGTTCCTG TCAACACTGAGCTTTGTCTGCCCCTGGTGTGC CACCAATCAG ATGGCTCACAATATTATCTACGGGCACGGGA TTATCATCTTCCTGAAAAATGTCAATGTCTTCC CTATCTTCCTGCAGATGGCACTGTGGAGGCC CTCCGACTCTACAGTGTACCTGCCCCCTCCAT CTGTCGCTCGAGTGGTCAGTACTGACGATTA CGTGTCCCGGACCTCTATCTTCTATCACGCAG GCAGCTCCAGACTGCTGACCGTGGGGAACCC TTATTTTAGGGTGGTCCCAAACGGCGCCGGG AATAAGCAGGCTGTGCCTAAAGTCAGCGCAT ACCAGTATCGGGTGTTCAGAGTCGCCCTGCC AGACCCCAACAAGTTTGGCCTGCCCGATTCCA CCATCTACAATCCTGAGACACAGCGACTGGT GTGGGCATGCGTCGGAATGGAAATCGGACG AGGCCAGCCACTGGGGATTGGACTGTCTGGC CACCCCTTCTACAACAAACTGGACGATACCGA GAGTGCACATGCCGCTACCGCCGTGATCACA CAGGACGTCCGCGATAATGTGTCCGTCGATT ATAAGCAGACTCAGCTGTGCATCCTGGGCTG TGTGCCAGCTATTGGGGAACATTGGGCAAAG GGAACCCTGTGCAAACCAGCACAGCTGCAGC CAGGGGACTGTCCACCTCTGGAGCTGAAGAA TACCATCATTGAAGACGGCGATATGGTGGAT ACAGGCTACGGGGCCATGGACTTTTCTACTCT GCAGGATACCAAGTGCGAGGTGCCTCTGGAC ATCTGCCAGAGCATTTGTAAATACCCTGATTA TCTGCAGATGTCAGCCGACCCATATGGCGAT AGCATGTTCTTTTGTCTGCGGAGAGAGCAGC TGTTCGCCAGGCACTTTTGGAACCGCGCTGG AGTGATGGGCGACACTGTCCCCACCGATCTG TACATCAAGGGAACAAGTGCCAATATGCGGG AAACTCCTGGCTCATGTGTGTATAGTCCTTCA CCAAGCGGGTCCATCATTACATCTGACAGTCA GCTGTTCAACAAGCCTTACTGGCTGCACAAA GCTCAGGGGCATAACAATGGAATTTGCTGGC ATAATCAGCTGTTTGTGACAGTGGTCGATAC CACACGGTCTACAAACCTGACTCTGTGTGCAT CCACTCAGAATCCCGTGCCTTCTACATACGAC CCAACTAAGTTCAAACAGTACAGCAGACACG TCGAGGAATATGATCTGCAGTTCATCTTTCAG CTGTGCACCATTACACTGACTGCCGAAGTGAT GAGCTACATCCATTCCATGAACTCTAGTATTC TGGAAAACTGGAATTTCGGCGTGCCACCCCC TCCAACTACCAGCCTGGTGGACACTTATAGAT TTGTCCAGTCCGTGGCTGTCACCTGTCAGAA GGATACAACTCCCCCTGAGAAACAGGACCCA TACGATAAGCTGAAATTCTGGACCGTGGACC TGAAGGAAAAATTTTCAAGCGACCTGGATCA GTATCCCCTGGGAAGGAAGTTTCTGGTGCAG GCAGGACTGAGGCGACGACCAACCATCGGG CCCCGGAAAAGACCTGCAGCCTCAACCAGCA CAGCTAGTACAGCATCACGCCCCGCTAAGAG GGTGCGCATTCGAAGCAAGAAA MHGPRETLQEIVLHLEPQNELDPVD LLCYEQLSESEEENDEADGVSHAQLP ARRAEPQRHKILCVCCKCDGRIELTV ESSAEDLRTLQQLFLSTLSFVCPWCA TNQ HPV45 L1 Page 35 of 48 MALWRPSDSTVYLPPPSVARVVSTD DYVSRTSIFYHAGSSRLLTVGNPYFRV VPNGAGNKQAVPKVSAYQYRVFRV ALPDPNKFGLPDSTIYNPETQRLVW ACVGMEIGRGQPLGIGLSGHPFYNK LDDTESAHAATAVITQDVRDNVSVD YKQTQLCILGCVPAIGEHWAKGTLCK PAQLQPGDCPPLELKNTIIEDGDMV DTGYGAMDFSTLQDTKCEVPLDICQ SICKYPDYLQMSADPYGDSMFFCLR REQLFARHFWNRAGVMGDTVPTDL YIKGTSANMRETPGSCVYSPSPSGSII TSDSQLFNKPYWLHKAQGHNNGIC WHNQLFVTVVDTTRSTNLTLCASTQ NPVPSTYDPTKFKQYSRHVEEYDLQF IFQLCTITLTAEVMSYIHSMNSSILEN WNFGVPPPPTTSLVDTYRFVQSVAV TCQKDTTPPEKQDPYDKLKFWTVDL KEKFSSDLDQYPLGRKFLVQAGLRRR PTIGPRKRPAASTSTASTASRPAKRV RIRSKK % Nucleotide Change 27.3% 29.6% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV45 L2 ATGGTATCCCACCGTGCAGCACGTCGCAAGC GGGCCTCTGCAACTGACTTATATAGAACATGT AAGCAATCCGGTACGTGCCCCCCTGATGTTAT TAACAAAGTGGAAGGCACAACCTTAGCTGAT AAAATTTTACAGTGGTCTAGCCTTGGGATATT TTTGGGTGGCCTTGGCATTGGTACCGGCAGT GGTTCTGGAGGCCGTACGGGCTATGTACCCT TAGGGGGCAGGTCTAATACTGTTGTGGATGT TGGCCCCACTAGGCCACCTGTGGTTATTGAA CCTGTAGGGCCTACTGATCCATCTATTGTTAC GTTGGTAGAGGATTCCAGTGTTGTTGCCTCT GGTGCTCCGGTTCCCACATTTACCGGAACCTC TGGGTTTGAAATTACGTCTTCTGGTACTACCA CACCAGCTGTGTTGGACATCACACCTACCGTG GACTCTGTTTCTATTTCGTCAACTAGTTTTACA AATCCTGCATTTTCTGATCCCTCTATTATTGAG GTGCCCCAAACAGGGGAGGTATCAGGTAATA TATTTGTTGGTACACCAACATCGGGCAGCCAT GGATATGAGGAAATACCTTTACAAACATTTG CATCTTCTGGGTCAGGTACGGAACCCATTAG TAGTACCCCCCTCCCTACTGTGCGGCGGGTAC GGGGTCCCCGCCTGTATAGTAGGGCTAATCA ACAGGTCCGTGTGTCCACCTCACAGTTTTTAA CACATCCCTCATCGTTGGTTACATTTGATAAT CCAGCTTATGAGCCCCTGGACACCACACTATC CTTTGAGCCTACCAGTAATGTTCCTGATTCCG ATTTTATGGATATTATTCGTTTGCATAGGCCA GCATTATCCTCTAGACGTGGCACTGTTAGATT TAGTAGATTGGGTCAAAGGGCAACCATGTTT ACACGTAGTGGTAAACAAATAGGGGGTAGG GTACATTTTTACCATGATATAAGCCCCATTGC TGCTACAGAGGAAATTGAATTGCAGCCTTTA ATTAGTGCTACAAATGATAGTGACCTGTTTGA TGTATATGCAGACTTCCCACCTCCTGCGTCCA CTACACCTAGCACTATACACAAATCATTTACA TATCCAAAGTATTCCTTGACCATGCCTTCTACT GCTGCATCCTCTTACAGTAATGTTACAGTACC ATTAACATCTGCATGGGATGTACCTATATATA CTGGCCCGGACATTATATTGCCATCCCATACT CCTATGTGGCCTAGTACATCTCCTACCAATGC TTCCACCACCACCTATATAGGTATTCATGGCA CACAATATTATTTATGGCCATGGTATTATTAT TTTCCTAAAAAACGTAAACGTATTCCCTATTTT TTTGCAGATGGCTTTGTGGCGGCCTAG ATGGTGTCACATAGAGCAGCCAGAAGAAAA AGGGCATCAGCAACCGACCTGTATAGAACCT GTAAGCAGAGCGGAACTTGTCCTCCCGACGT GATCAACAAGGTCGAGGGAACCACACTGGCC GATAAAATTCTGCAGTGGAGCTCCCTGGGAA TCTTCCTGGGAGGACTGGGAATTGGAACCGG AAGTGGATCAGGAGGACGAACAGGATATGT CCCACTGGGAGGAAGAAGCAATACAGTGGT CGACGTGGGCCCTACTAGGCCACCTGTGGTC ATCGAGCCAGTCGGACCTACCGACCCATCCA TTGTGACACTGGTCGAAGATTCTAGTGTGGT CGCATCCGGAGCTCCAGTGCCAACCTTCACA GGAACTTCTGGCTTTGAGATCACCTCAAGCG GAACTACCACACCTGCCGTGCTGGACATCAC CCCAACAGTGGATAGCGTCTCCATTTCCTCTA CAAGCTTCACTAACCCTGCTTTTAGTGATCCA TCAATCATTGAGGTGCCTCAGACCGGAGAAG TCAGCGGCAATATCTTCGTGGGCACTCCAACC TCTGGGAGTCACGGATACGAGGAAATTCCCC TGCAGACTTTTGCCAGTTCAGGCTCCGGGAC CGAACCAATCAGCTCCACACCTCTGCCAACTG TGCGGAGAGTCAGAGGGCCCAGGCTGTATTC TAGGGCAAACCAGCAGGTGCGCGTCTCAACA AGCCAGTTCCTGACTCACCCCTCTAGTCTGGT GACATTTGACAACCCAGCCTACGAGCCCCTG GATACTACCCTGTCTTTCGAACCCACCAGTAA TGTGCCTGACTCAGATTTTATGGACATCATTC GCCTGCATCGACCTGCTCTGTCAAGCAGGCG AGGAACCGTGCGGTTCAGTAGACTGGGACA GCGAGCAACCATGTTTACACGAAGCGGCAAG CAGATCGGAGGACGAGTGCACTTCTATCATG ATATCAGCCCTATTGCCGCTACAGAGGAAAT CGAGCTGCAGCCACTGATTAGCGCCACCAAT GACTCCGATCTGTTCGACGTGTACGCAGATTT TCCACCCCCTGCCAGCACAACTCCATCCACTA TTCATAAGAGCTTTACCTATCCCAAATACTCTC TGACCATGCCTAGTACAGCAGCCTCCTCTTAT TCAAACGTGACTGTCCCTCTGACCAGCGCTTG GGACGTGCCAATCTACACAGGCCCCGATATC ATTCTGCCTTCCCACACTCCCATGTGGCCTTCC ACTTCTCCAACCAATGCATCTACCACAACTTA TATCGGCATTCATGGGACCCAGTACTATCTGT GGCCATGGTACTATTACTTCCCCAAGAAACG AAAACGGATCCCCTACTTCTTTGCTGACGGCT TTGTGGCTGCA MVSHRAARRKRASATDLYRTCKQSG TCPPDVINKVEGTTLADKILQWSSLG IFLGGLGIGTGSGSGGRTGYVPLGGR SNTVVDVGPTRPPVVIEPVGPTDPSI VTLVEDSSVVASGAPVPTFTGTSGFE ITSSGTTTPAVLDITPTVDSVSISSTSF TNPAFSDPSIIEVPQTGEVSGNIFVGT PTSGSHGYEEIPLQTFASSGSGTEPIS STPLPTVRRVRGPRLYSRANQQVRV STSQFLTHPSSLVTFDNPAYEPLDTTL SFEPTSNVPDSDFMDIIRLHRPALSSR RGTVRFSRLGQRATMFTRSGKQIGG RVHFYHDISPIAATEEIELQPLISATND SDLFDVYADFPPPASTTPSTIHKSFTY PKYSLTMPSTAASSYSNVTVPLTSAW DVPIYTGPDIILPSHTPMWPSTSPTN ASTTTYIGIHGTQYYLWPWYYYFPKK RKRIPYFFADGFVAA Page 36 of 48 % Nucleotide Change 28.0% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV51 E1 ATGGACTGTGAAGGTACAGAGGATGAGGGG GCGGGGTGTAATGGGTGGTTTTTTGTTGAAG CAATAGTAGAAAAAAAAACAGGAGATAATGT TTCGGATGATGAGGATGAAAATGCAGATGAT ACAGGATCTGATTTAATAAACTTTATAGATAG TGAAACTAGTATTTGCAGTCAGGCGGAACAG GAGACAGCACGGGCGTTGTTTCAGGCCCAAG AATTACAGGCAAACAAAGAGGCTGTGCATCA GTTAAAACGAAAGTTTCTAGTCAGCCCGCGA AGCAGCCCATTAGGAGACATTACAAATCAAA ACAACACACACAGCCATAGTCAGGCAAACGA GTCACAAGTTAAAAGGAGATTACTGGACAGT TATCCGGACAGCGGATATGGCAATACACAAG TGGAAACTGTGGAAGCAACGTTGCAGGTAG ATGGGCAACATGGCGGTTCACAGAACAGTGT GTGTAGTAGCGGGGGGGGCAGTGTTATGGA TGTGGAAACAACAGAAAGCTGTGCAAATGTA GAACTAAACAGTATATGTGAAGTATTAAAAA GCAGTAATGCAAAAGCAACGTTAATGGCAAA ATTTAAAGAGTTGTATGGTATTAGTTATAATG AGTTGGTACGGGTGTTTAAAAGTGATAAAAC ATGTTGTATAGATTGGGTTTGTGCATTGTTTG GCGTTTCCCCAATGGTAGCAGAAAATTTAAA AACACTAATTAAGCCATTTTGCATGTACTACC ATATACAATGTTTATCATGTGATTGGGGCACC ATTGTATTAATGCTAATTAGGTTTTCATGTGC AAAAAACAGAACAACAATTGCTAAGTGTTTA AGTACATTAGTAAATATCCCACAATCACAAAT GTTTATAGAACCACCAAAATTACGTAGTACAC CTGTGGCATTATATTTTTATAGAACAGGCATA TCAAACATTAGCAATACATATGGAGAGACAC CTGAATGGATTACACGACAAACGCAACTACA ACATAGTTTTGAGGATAGTACCTTTGAATTAT CACAAATGGTGCAATGGGCATTTGACCATGA AGTATTAGATGATAGTGAAATAGCATTTCATT ATGCACAATTAGCAGATATAGATAGTAATGC TGCAGCGTTTTTAAAGAGTAATTGCCAAGCA AAATATGTAAAAGATTGTGGGACCATGGCAC GGCATTACAAACGAGCACAAAGAAAATCATT ATCTATGTCAGCCTGGATAAGGTATAGATGT GATAGAGCAAAGGATGGAGGCAACTGGAGA GAAATTGCTAAATTTTTAAGATATCAAGGTGT AAACTTTATGTCCTTTATTCAAATGTTTAAACA GTTTTTAAAAGGAACACCAAAACACAATTGC ATAGTCATATATGGCCCACCAAACACAGGCA AGTCATTATTTGCAATGAGCCTAATGAAGTTT ATGCAAGGGTCCATTATTTCATATGTAAACTC TGGTAGTCATTTTTGGTTACAGCCACTAGAG GATGCTAAAATAGCATTGTTAGATGATGCTA CGTATGGGTGTTGGACATATATTGATCAGTA TTTAAGAAACTTTTTAGATGGTAATCCATGTA GTATAGATAGAAAACATAGGAGTTTAATACA ATTAGTATGTCCACCATTACTAATAACGTCAA ACATAAATCCACAAGAGGATGCAAACCTAAT GTATTTACATACAAGGGTAACAGTATTAAAG TTTTTAAATACATTTCCATTTGATAACAATGG GAATGCTGTGTATACATTGAATGATGAAAAT TGGAAAAATTTTTTTTCCACCACATGGTCCAG ATTAGATTTGGAGGAGGAAGAGGACAAAGA AAATGGAGACCCTATGCCACCGTTTAAATGT GTGCCAGGAGAAAATACTAGACTGTTATGA ATGGACTGCGAGGGAACTGAGGATGAGGGG GCTGGGTGTAACGGCTGGTTTTTCGTGGAGG CTATTGTGGAGAAGAAGACTGGGGATAATGT GAGCGACGATGAGGACGAAAACGCCGACGA TACCGGCTCCGACCTGATCAATTTCATTGATA GTGAGACCTCAATCTGCAGCCAGGCAGAGCA GGAAACAGCCCGGGCTCTGTTTCAGGCTCAG GAGCTGCAGGCAAACAAGGAAGCCGTGCAC CAGCTGAAGCGCAAATTCCTGGTCTCTCCACG AAGCTCCCCCCTGGGAGATATTACCAATCAG AACAATACACACAGCCATTCTCAGGCAAACG AGAGCCAGGTGAAGCGGAGACTGCTGGACT CTTACCCTGATAGTGGGTATGGAAATACACA GGTGGAGACTGTCGAAGCAACCCTGCAGGT GGACGGACAGCATGGAGGGTCCCAGAACTC TGTCTGTTCTAGTGGAGGCGGGAGCGTGATG GATGTCGAGACCACAGAATCTTGCGCTAATG TGGAGCTGAACAGTATCTGTGAAGTCCTGAA ATCAAGCAATGCAAAGGCCACTCTGATGGCA AAGTTCAAAGAGCTGTACGGCATCAGTTATA ACGAACTGGTGAGGGTCTTTAAGTCAGACAA AACCTGCTGTATTGATTGGGTGTGCGCACTG TTCGGGGTGTCCCCTATGGTGGCCGAGAACC TGAAGACCCTGATCAAACCTTTCTGTATGTAC TACCACATCCAGTGCCTGTCTTGTGACTGGG GGACAATCGTGCTGATGCTGATTCGGTTCAG TTGCGCCAAAAATAGAACTACCATCGCTAAG TGTCTGTCAACCCTGGTGAACATCCCACAGA GCCAGATGTTTATTGAACCCCCTAAGCTGCGA TCCACACCCGTCGCCCTGTACTTCTATCGGAC TGGAATCAGTAACATTTCAAATACCTACGGC GAGACTCCAGAATGGATCACCCGCCAGACAC AGCTGCAGCACTCATTCGAGGACAGCACATT TGAACTGAGCCAGATGGTGCAGTGGGCATTC GATCACGAGGTCCTGGACGATTCCGAAATCG CCTTTCATTACGCTCAGCTGGCAGACATTGAT TCCAATGCCGCTGCATTTCTGAAGTCTAACTG CCAGGCCAAGTACGTGAAAGACTGTGGCACT ATGGCTAGACATTATAAACGAGCACAGCGGA AGAGTCTGTCAATGAGCGCTTGGATCAGATA CCGGTGCGACAGGGCAAAAGATGGAGGCAA TTGGAGGGAGATCGCCAAGTTCCTGCGCTAT CAGGGGGTGAACTTCATGTCTTTTATTCAGAT GTTCAAGCAGTTTCTGAAAGGAACACCCAAG CACAATTGTATCGTCATCTACGGCCCACCCAA CACTGGGAAAAGTCTGTTCGCTATGTCACTG ATGAAGTTTATGCAGGGGAGCATCATTAGCT ACGTGAACAGCGGATCTCATTTTTGGCTGCA GCCCCTGGAGGACGCCAAAATCGCTCTGCTG GACGATGCCACTTACGGATGCTGGACCTACA TTGATCAGTATCTGCGGAATTTCCTGGACGG CAACCCTTGCAGCATCGATAGAAAGCACAGG TCCCTGATTCAGCTGGTGTGTCCTCCACTGCT GATCACAAGCAACATTAATCCTCAGGAGGAC GCCAACCTGATGTACCTGCATACTAGAGTGA CCGTCCTGAAATTTCTGAATACTTTCCCATTTG ACAACAATGGCAACGCTGTGTATACCCTGAA CGATGAAAATTGGAAGAACTTCTTTTCCACAA CTTGGTCTCGCCTGGATCTGGAGGAAGAGGA AGACAAAGAGAATGGCGATCCTATGCCCCCT TTCAAGTGCGTGCCAGGGGAAAACACCCGAC TGCTG MDCEGTEDEGAGCNGWFFVEAIVE KKTGDNVSDDEDENADDTGSDLINF IDSETSICSQAEQETARALFQAQELQ ANKEAVHQLKRKFLVSPRSSPLGDIT NQNNTHSHSQANESQVKRRLLDSYP DSGYGNTQVETVEATLQVDGQHGG SQNSVCSSGGGSVMDVETTESCAN VELNSICEVLKSSNAKATLMAKFKELY GISYNELVRVFKSDKTCCIDWVCALF GVSPMVAENLKTLIKPFCMYYHIQCL SCDWGTIVLMLIRFSCAKNRTTIAKC LSTLVNIPQSQMFIEPPKLRSTPVALY FYRTGISNISNTYGETPEWITRQTQL QHSFEDSTFELSQMVQWAFDHEVL DDSEIAFHYAQLADIDSNAAAFLKSN CQAKYVKDCGTMARHYKRAQRKSL SMSAWIRYRCDRAKDGGNWREIAK FLRYQGVNFMSFIQMFKQFLKGTPK HNCIVIYGPPNTGKSLFAMSLMKFM QGSIISYVNSGSHFWLQPLEDAKIALL DDATYGCWTYIDQYLRNFLDGNPCS IDRKHRSLIQLVCPPLLITSNINPQED ANLMYLHTRVTVLKFLNTFPFDNNG NAVYTLNDENWKNFFSTTWSRLDLE EEEDKENGDPMPPFKCVPGENTRLL Page 37 of 48 % Nucleotide Change 26.9% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV51 E2 ATGGAGACCCTATGCCACCGTTTAAATGTGT GCCAGGAGAAAATACTAGACTGTTATGAACT GGACAGTGATAAATTAGTAGATCAAATTAAC TATTGGACATTGTTACGATATGAAGCTGCTAT GTTTTATGCAGCACGGGAAAGAAACTTACGA ACAATCAATCACCAGGTAGTACCAGCAACAA CAGTATCAAAACAAAAGGCCTGTCAAGCAAT TGAAATGCACATGGCCTTACAATCGCTTAACA AATCAGACTATAACATGGAACCATGGACAAT GCGGGAGACATGTTATGAACTATGGTGTGTG GCTCCCAAGCAATGTTTCAAAAAGGGGGGCA TAACTGTAACAGTTATATTTGATGGAAATAAG GACAATGCAATGGACTATACAAGCTGGAAAT TTATATATATATATGATAATGATAAGTGGGTA AAGACAAATGGAAATGTGGACTATACGGGTA TATATTACACTGTAAATTCAAAAAAAGAATAT TATGTACAGTTTAAAGATGAAGCCAAAATAT ATGGGGCACAACAGTGGGAGGTCTATATGTA TGGTACTGTAATAACATGTCCTGAATATGTAT CTAGTACCTGCAGCGACGCGTTATCCACTACT ACAACTGTTGAACAACTATCAAACACCCCAAC GACCAATCCCCTTACCACCTGCGTGGGCGCC AAAGAAGCCCAGACACAACAGCGAAAACGA CAGCGACTTACTGAGCCCGACTCCTCCACAAT CTCCCCACTGTCCGTGGACAATACAAACAACC AAATACACTGTGGAAGTGGAAGCACTAACAC TGGAGGGCACCAAAGTGCAACTCAGACTGCG TTTATAGTGCATTTAAAAGGTGATACAAATTG TTTAAAATGTTTTAGATACAGATTTACAAAAC ACAAAGGGTTATATAAAAACGTATCCTCAAC CTGGCATTGGACCAGTAATACTAAAACAGGC ATTGTTACCATTGTGTTTGACAGTGCACATCA ACGGGAAACATTTATAAAAACCATTAAAGTA CCCCCAAGTGTAACACTGTCATTGGGAATTAT GACACTGTAA ATGTATCTAGTACCTGCAGCGACGCGTTATCC ACTACTACAACTGTTGAACAACTATCAAACAC CCCAACGACCAATCCCCTTACCACCTGCGTGG GCGCCAAAGAAGCCCAGACACAACAGCGAA AACGACAGCGACTTACTGAGCCCGACTCCTC CACAATCTCCCCACTGTCCGTGGACAATACAA ACAACCAAATACACTGTGGAAGTGGAAGCAC TAACACTGGAGGGCACCAAAGTGCAACTCAG ACTGCGTTTATAG ATGTATAGACATATTGTAACCATTGCAGTGTT TATTATTTTGCTATTTGTGCTTTGCTTGTGTGT GTGTCTTGTGTTGTGTTGTTTGTTGCCGCTAC TGCTGTCCCAATACGTGTTTGCAGCTGCCTTA TTATTAATTTTATGTTTTTGGTTTGTTGTTGCA ACATCCCAATTAACTACATTTTTTGTATATTTG ATTTTTTTTTACTTACCTTGTTTACTTTTACATC TATATACATTTTTACTTTTGCAATAA ATGTTCGAAGACAAGAGGGAAAGACCACGA ACGCTGCATGAATTATGTGAAGCTTTGAACG TTTCTATGCACAATATACAGGTAGTGTGTGTG TATTGTAAAAAGGAATTATGTAGAGCAGATG TATATAATGTAGCATTTACTGAAATTAAGATT GTATATAGGGATAATAATCCATATGCAGTAT GCAAACAATGTTTACTGTTTTATTCAAAAATT AGAGAGTATAGACGTTATAGCAGGTCTGTGT ATGGTACTACATTAGAGGCAATTACTAAAAA AAGCTTATATGATTTATCGATAAGGTGTCATA GATGTCAAAGACCACTTGGGCCTGAAGAAAA GCAAAAATTGGTGGACGAAAAAAAAAGGTT CCATGAAATAGCGGGACGTTGGACGGGGCA ATGCGCTAATTGCTGGCAACGTACACGACAA CGTAACGAAACCCAAGTGTAA ATGGAAACTCTGTGTCATAGACTGAACGTGT GTCAGGAAAAGATTCTGGACTGCTACGAACT GGACTCCGATAAACTGGTGGATCAGATCAAC TACTGGACACTGCTGAGATATGAGGCCGCTA TGTTCTACGCAGCCCGGGAAAGAAACCTGCG CACCATCAATCATCAGGTGGTCCCAGCCACCA CAGTGTCTAAGCAGAAGGCCTGCCAGGCAAT TGAGATGCACATGGCTCTGCAGAGCCTGAAC AAGTCCGATTATAATATGGAACCCTGGACTAT GCGAGAGACCTGCTACGAACTGTGGTGTGTG GCACCTAAGCAGTGTTTCAAGAAAGGCGGGA TCACAGTGACTGTCATTTTTGACGGCAACAAG GATAATGCTATGGACTATACAAGTTGGAAAT TCATCTACATCTACGACAACGATAAGTGGGT GAAAACTAACGGCAATGTCGATTACACTGGG ATCTACTATACCGTGAATAGCAAGAAAGAGT ACTATGTCCAGTTTAAGGACGAAGCTAAAAT CTATGGAGCACAGCAGTGGGAGGTGTACAT GTATGGCACCGTCATTACATGCCCCGAATAC GTGAGCTCCACCTGTTCTGACGCCCTGAGTAC TACCACAACTGTCGAGCAGCTGAGCAACACA CCAACCACAAATCCCCTGACTACCTGCGTGG GAGCAAAGGAGGCTCAGACCCAGCAGAGGA AACGCCAGCGACTGACAGAACCTGATTCTAG TACTATCAGTCCACTGTCAGTGGACAACACCA ACAATCAGATTCACTGTGGGTCCGGATCTACC AATACAGGAGGCCATCAGTCCGCAACTCAGA CCGCCTTTATCGTGCACCTGAAGGGGGATAC TAATTGCCTGAAATGTTTCCGGTACAGATTCA CCAAGCATAAAGGACTGTACAAGAACGTGTC AAGCACATGGCACTGGACTTCCAATACAAAA ACTGGCATCGTGACCATTGTCTTCGACTCTGC CCATCAGAGGGAGACCTTTATCAAGACCATC AAGGTGCCCCCTTCAGTCACCCTGAGCCTGG GGATTATGACACTG ATGTACCTGGTCCCTGCCGCTACCCGCTATCC CCTGCTGCAGCTGCTGAACAACTATCAGACTC CACAGAGGCCCATTCCCCTGCCACCCGCCTG GGCTCCAAAGAAACCCAGGCACAACAGCGA GAATGACTCCGATCTGCTGTCTCCTACCCCCC CTCAGAGCCCTCATTGCCCCTGGACAATCCAG ACCACAAAGTACACTGTGGAGGTCGAAGCCC TGACTCTGGAAGGCACCAAAGTGCAGCTGCG GCTGAGACTG ATGTATCGCCATATTGTGACTATCGCCGTGTT CATCATTCTGCTGTTCGTGCTGTGCCTGTGTG TGTGTCTGGTGCTGTGCTGCCTGCTGCCCCTG CTGCTGAGCCAGTACGTGTTCGCCGCTGCAC TGCTGCTGATCCTGTGCTTCTGGTTTGTGGTC GCCACTTCCCAGCTGACCACATTCTTTGTCTA CCTGATTTTCTTTTATCTGCCTTGTCTGCTGCT GCACCTGTATACCTTTCTGCTGCTGCAG ATGTTTGAAGACAAGCGGGAACGCCCAAGG ACTCTGCACGAACTGTGTGAAGCACTGAATG TGAGCATGCATAATATCCAGGTGGTGTGCGT GTACTGTAAGAAAGAGCTGTGCCGGGCCGAC GTGTATAACGTCGCTTTCACCGAAATCAAGAT TGTGTACAGAGATAACAATCCCTATGCTGTCT GCAAGCAGTGTCTGCTGTTTTACTCCAAAATC CGCGAGTACCGGAGATATAGCCGATCCGTGT ACGGAACCACACTGGAAGCCATCACAAAGAA ATCTCTGTATGACCTGAGTATTCGGTGCCACA GATGTCAGAGGCCCCTGGGCCCTGAGGAAA AGCAGAAACTGGTGGATGAGAAGAAAAGGT TCCATGAAATTGCAGGACGATGGACTGGACA GTGCGCAAACTGTTGGCAGAGGACTCGCCAG CGAAATGAGACCCAGGTC METLCHRLNVCQEKILDCYELDSDKL VDQINYWTLLRYEAAMFYAARERNL RTINHQVVPATTVSKQKACQAIEMH MALQSLNKSDYNMEPWTMRETCY ELWCVAPKQCFKKGGITVTVIFDGN KDNAMDYTSWKFIYIYDNDKWVKT NGNVDYTGIYYTVNSKKEYYVQFKD EAKIYGAQQWEVYMYGTVITCPEYV SSTCSDALSTTTTVEQLSNTPTTNPLT TCVGAKEAQTQQRKRQRLTEPDSST ISPLSVDNTNNQIHCGSGSTNTGGH QSATQTAFIVHLKGDTNCLKCFRYRF TKHKGLYKNVSSTWHWTSNTKTGIV TIVFDSAHQRETFIKTIKVPPSVTLSLG IMTL HPV51 E4 HPV51 E5 HPV51 E6 Page 38 of 48 % Nucleotide Change 25.1% MYLVPAATRYPLLQLLNNYQTPQRPI PLPPAWAPKKPRHNSENDSDLLSPT PPQSPHCPWTIQTTKYTVEVEALTLE GTKVQLRLRL 27.6% MYRHIVTIAVFIILLFVLCLCVCLVLCC LLPLLLSQYVFAAALLLILCFWFVVAT SQLTTFFVYLIFFYLPCLLLHLYTFLLLQ *X 27.5% MFEDKRERPRTLHELCEALNVSMHN IQVVCVYCKKELCRADVYNVAFTEIKI VYRDNNPYAVCKQCLLFYSKIREYRR YSRSVYGTTLEAITKKSLYDLSIRCHRC QRPLGPEEKQKLVDEKKRFHEIAGR WTGQCANCWQRTRQRNETQV 26.3% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV51 E7 ATGCGTGGTAATGTACCACAATTAAAAGATG TAGTATTGCATTTAACACCACAGACTGAAATT GACTTGCAATGCTACGAGCAATTTGACAGCT CAGAGGAGGAGGATGAAGTAGATAATATGC GTGACCAGCTACCAGAAAGACGGGCTGGAC AGGCTACGTGTTACAGAATTGAAGCTCCGTG TTGCAGGTGTTCAAGTGTAGTACAACTGGCA GTGGAAAGCAGTGGAGACACCCTTCGCGTTG TACAGCAGATGTTAATGGGCGAACTAAGCCT GGTTTGCCCGTGTTGTGCGAACAACTAG ATGGCATTGTGGCGCACTAATGACAGCAAGG TGTATTTGCCACCTGCACCTGTGTCTCGAATT GTGAATACAGAAGAATATATCACACGCACCG GCATATATTACTATGCAGGCAGTTCCAGACTA ATAACATTAGGACATCCCTATTTTCCAATACC TAAAACCTCAACGCGTGCTGCTATTCCTAAAG TATCTGCATTTCAATACAGGGTATTTAGGGTA CAGTTACCAGATCCTAACAAGTTTGGACTCCC GGATCCAAATTTATATAATCCAGACACAGATA GGTTGGTGTGGGGTTGTGTGGGCGTTGAGG TGGGCAGAGGACAGCCCCTTGGTGTTGGCCT TAGTGGTCATCCCTTATTTAATAAATATGATG ACACAGAAAATTCACGCATAGCAAATGGCAA TGCACAACAAGATGTTAGAGATAACACATCT GTTGACAACAAACAGACTCAGTTATGTATAAT AGGCTGTGCTCCACCTATTGGGGAACACTGG GGTATTGGCACTACATGCAAAAACACACCTG TACCTCCAGGAGACTGCCCCCCCCTGGAACTT GTATCCTCTGTCATTCAGGATGGCGATATGAT TGATACAGGGTTTGGAGCTATGGATTTCGCT GCCCTACAGGCCACCAAATCAGACGTCCCTTT GGATATTTCACAGTCTGTTTGTAAATATCCTG ATTATTTAAAAATGTCTGCAGACACATATGGT AATTCCATGTTTTTTCATTTACGCAGGGAGCA AATCTTTGCTAGGCACTATTATAATAAACTTG TAGGTGTTGGGGAAGACATTCCTAACGATTA TTATATTAAGGGTAGTGGTAATGGCCGTGAC CCTATAGAAAGTTATATATACTCTGCTACTCC CAGTGGGTCTATGATAACATCTGATTCTCAAA TTTTTAATAAGCCTTATTGGCTCCACCGTGCG CAGGGTCACAATAATGGCATTTGCTGGAACA ATCAGCTTTTTATTACCTGTGTTGATACTACCA GAAGTACAAATTTAACTATTAGCACTGCCACT GCTGCGGTTTCCCCAACATTTACTCCAAGTAA CTTTAAGCAATATATTAGGCATGGGGAAGAG TATGAATTGCAATTTATTTTTCAATTATGTAAA ATTACTTTAACTACAGAGGTAATGGCTTATTT ACACACAATGGATCCTACCATTCTTGAACAGT GGAATTTTGGATTAACATTACCTCCGTCTGCT AGTTTGGAGGATGCATATAGGTTTGTTAGAA ATGCAGCTACTAGCTGTCAAAAGGACACCCC TCCACAGGCTAAGCCAGATCCTTTGGCCAAAT ATAAATTTTGGGATGTTGATTTAAAGGAACG ATTTTCTTTAGATTTAGACCAATTTGCATTGG GTCGCAAGTTTTTGTTGCAGGTTGGCGTACA ACGCAAGCCCAGACCAGGCCTTAAACGCCCG GCCTCATCGGCATCCTCTTCCTCTTCCTCTTCA GCCAAACGTAAACGTGTTAAAAAGTAA ATGAGAGGCAACGTCCCCCAGCTGAAAGATG TCGTCCTGCACCTGACTCCACAGACCGAGATT GACCTGCAGTGTTATGAACAGTTTGACAGCT CCGAGGAAGAGGACGAAGTGGATAACATGC GAGATCAGCTGCCAGAGCGAAGAGCAGGAC AGGCTACCTGCTACAGGATCGAAGCACCTTG CTGTCGCTGTTCTAGTGTGGTCCAGCTGGCC GTGGAGTCAAGCGGCGACACACTGAGAGTG GTCCAGCAGATGCTGATGGGGGAACTGTCTC TGGTCTGCCCCTGCTGTGCTAACAAT ATGGCTCTGTGGCGAACTAATGACTCTAAGG TCTATCTGCCCCCTGCCCCCGTCTCCCGAATC GTGAATACTGAAGAATACATCACCCGGACAG GAATCTACTATTACGCTGGCAGCTCCAGACTG ATCACTCTGGGGCACCCTTATTTCCCTATTCC AAAGACCTCAACACGGGCCGCTATCCCAAAA GTGTCTGCATTCCAGTACCGAGTCTTTCGGGT GCAGCTGCCCGACCCTAACAAGTTTGGACTG CCAGATCCCAACCTGTATAATCCAGACACAG ATCGACTGGTCTGGGGATGCGTGGGAGTCG AAGTGGGACGAGGGCAGCCTCTGGGAGTGG GCCTGAGTGGACACCCACTGTTCAACAAGTA CGACGATACTGAAAATTCTAGGATCGCTAAC GGCAATGCACAGCAGGACGTCCGCGATAACA CCTCCGTGGACAATAAGCAGACACAGCTGTG CATCATTGGGTGTGCCCCCCCTATCGGAGAG CATTGGGGGATTGGAACCACATGCAAAAATA CCCCCGTGCCACCAGGCGATTGTCCTCCACTG GAACTGGTCTCTAGTGTGATCCAGGACGGGG ATATGATTGACACCGGCTTCGGGGCTATGGA TTTTGCAGCCCTGCAGGCAACAAAGTCCGAC GTCCCACTGGATATTAGTCAGTCAGTGTGTA AGTATCCCGACTACCTGAAAATGTCTGCCGAT ACCTACGGCAACAGCATGTTCTTTCACCTGCG GAGAGAGCAGATTTTCGCTCGGCATTATTAC AATAAGCTGGTCGGAGTGGGCGAAGACATC CCCAACGATTATTACATCAAGGGGAGCGGAA ATGGCAGAGACCCCATCGAGTCCTATATCTAC TCTGCAACTCCTAGCGGCTCCATGATCACCTC TGATAGTCAGATTTTCAACAAGCCTTACTGGC TGCACCGAGCACAGGGACATAACAATGGGAT CTGCTGGAACAATCAGCTGTTTATTACCTGTG TGGACACTACCCGAAGTACCAACCTGACAAT TTCAACTGCCACCGCTGCAGTGAGCCCAACAT TCACTCCCTCCAATTTTAAGCAGTATATCAGG CACGGCGAGGAATACGAGCTGCAGTTCATCT TTCAGCTGTGCAAAATTACTCTGACAACTGAA GTGATGGCCTACCTGCATACTATGGACCCTAC CATCCTGGAACAGTGGAACTTCGGACTGACC CTGCCACCTTCAGCCAGCCTGGAGGATGCTT ATAGATTTGTGAGGAATGCCGCTACATCCTG TCAGAAGGACACTCCACCCCAGGCAAAACCT GATCCACTGGCCAAGTACAAATTCTGGGACG TGGATCTGAAGGAACGGTTCAGCCTGGACCT GGACCAGTTCGCCCTGGGCAGGAAATTTCTG CTGCAGGTCGGAGTGCAGCGAAAGCCACGA CCTGGACTGAAACGCCCCGCATCAAGCGCCT CCTCTAGTTCAAGCTCCTCTGCTAAGAGGAAA CGCGTGAAGAAA MRGNVPQLKDVVLHLTPQTEIDLQC YEQFDSSEEEDEVDNMRDQLPERRA GQATCYRIEAPCCRCSSVVQLAVESS GDTLRVVQQMLMGELSLVCPCCAN N*X HPV51 L1 Page 39 of 48 MALWRTNDSKVYLPPAPVSRIVNTE EYITRTGIYYYAGSSRLITLGHPYFPIPK TSTRAAIPKVSAFQYRVFRVQLPDPN KFGLPDPNLYNPDTDRLVWGCVGV EVGRGQPLGVGLSGHPLFNKYDDTE NSRIANGNAQQDVRDNTSVDNKQT QLCIIGCAPPIGEHWGIGTTCKNTPV PPGDCPPLELVSSVIQDGDMIDTGF GAMDFAALQATKSDVPLDISQSVCK YPDYLKMSADTYGNSMFFHLRREQI FARHYYNKLVGVGEDIPNDYYIKGSG NGRDPIESYIYSATPSGSMITSDSQIF NKPYWLHRAQGHNNGICWNNQLFI TCVDTTRSTNLTISTATAAVSPTFTPS NFKQYIRHGEEYELQFIFQLCKITLTTE VMAYLHTMDPTILEQWNFGLTLPPS ASLEDAYRFVRNAATSCQKDTPPQA KPDPLAKYKFWDVDLKERFSLDLDQ FALGRKFLLQVGVQRKPRPGLKRPAS SASSSSSSSAKRKRVKK % Nucleotide Change 25.9% 24.8% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV51 L2 ATGGTGGCTACACGTGCACGGCGTCGGAAG CGAGCATCTGTAACACAATTATATTCTACATG CAAAGCTGCTGGTACATGTCCTCCTGATGTTG TGAATAAGGTTGAAGGTACTACATTGGCCGA TAAAATATTACAGTGGAGTGGGTTGGGTATA TTTTTGGGTGGCCTAGGTATTGGTACTGGGT CTGGATCTGGGGGGCGTACTGGATATATCCC TTTAGGTGGTGGGGGTCGCCCAGGCGTGGT GGATATTGCTCCTGCAAGGCCACCTATTATAA TTGACCTATGGCACCATACTGAACCTTCTATA GTAAATTTGGTTGAGGACTCTAGTATTATTCA GTCTGGGTCTCCTATACCTACCTTTACTGGTA CCGATGGCTTTGAAATTACTTCATCTTCCACA ACAACCCCTGCTGTGTTGGACATCACCCCATC TGCTGGTACTGTACATGTTTCTAGTACTAACA TTGAAAATCCTTTATATATTGAACCTCCATCCA TTGAGGCTCCACAATCTGGAGAAGTGTCAGA TATATATTTACTAGTACACTACTCTGGTACTC ATGGGTATGAAGAAATACCTATGGAAGTGTT TGCATCCAATGTCAGTACTGGTACTGAACCTA TTAGCAGCACACCTACTCCAGGGGTTAGTCG CATAGCTGCTCCCCGCTTGTATAGTAAGTCCT ACACACAGGTTAAAGTTACAAATCCTGATTTT ATTAGTAAGCCATCCACATTTGTTACATTTAA TAATCCTGCTTTTGAGCCTATTGACACATCCA TAACTTTTGAGGAACCTGATGCTGTTGCACCT GATCCTGATTTTCTGGATATTATTACACTGCA CCGCCCTGCCCTTACATCTCGTAGAGGCACA GTACGCTTTAGTAGGTTAGGTCAAAAGGCCA CCATGCGCACTCGTAGTGGCAAACAAATTGG TGCTCGTGTACATTATTATCATGATATTAGTA GAATTGCACCAGCTGATGAACTTGAAATGCA GCCTTTACTTTCACCTTCTAATAATTATAGTTA TGACATTTATGCTGATTTAGATGAAGCTGAAA CAGGTTTTATACAGCCCACACACACCACACCT ATGTCACACTCCTCTTTGTCTAGGCAGTTGCC CTCCTTATCTTCATCTATGTCTTCATCTTATGC AAATGTTACTATTCCATTTTCAACTACATATTC TGTTCCTATTCATACAGGGCCTGATGTGGTAT TGCCCACATCTCCTACAGTATGGCCTTATGTT CCCCACACTTCCATTGACACCAAGCATTCTAT TGTTATACTAGGTGGGGATTACTATTTGTGGC CCTATACACATTTACTACGCAAACGCCGTAAA CGTATACCCTATTTTTTTACAGATGGCATTGT GGCGCACTAA ATGGTCGCAACTCGGGCAAGAAGAAGGAAA AGAGCCTCAGTCACTCAGCTGTATAGCACCT GTAAAGCCGCCGGAACCTGTCCCCCAGACGT GGTCAACAAGGTGGAGGGCACCACACTGGC CGATAAAATTCTGCAGTGGAGCGGGCTGGG AATTTTCCTGGGAGGACTGGGAATCGGGACA GGATCTGGCAGTGGAGGCCGGACTGGATAC ATTCCACTGGGAGGAGGCGGGCGACCTGGA GTGGTGGACATCGCACCAGCCCGCCCCCCTA TCATTATCGATCTGTGGCACCATACCGAGCCC AGCATCGTGAATCTGGTCGAAGACAGCTCCA TTATCCAGTCAGGCAGCCCTATTCCAACCTTC ACAGGGACTGACGGATTTGAGATCACCTCTA GTTCAACTACCACACCCGCTGTGCTGGATATT ACTCCTTCCGCAGGGACCGTGCACGTCAGCT CCACAAACATCGAGAATCCACTGTACATTGA GCCACCCTCTATCGAAGCACCCCAGTCAGGA GAAGTGAGCGATATCTACCTGCTGGTCCACT ATAGTGGCACCCACGGCTATGAGGAAATCCC TATGGAGGTGTTCGCCAGCAACGTCTCCACC GGAACAGAACCTATTTCTAGTACTCCAACCCC TGGCGTGTCTCGCATCGCAGCTCCTCGACTGT ACTCCAAGTCTTATACACAGGTGAAAGTCACT AATCCTGACTTTATCTCTAAGCCAAGTACATT CGTGACTTTTAACAATCCAGCTTTCGAGCCCA TTGACACCTCCATCACATTTGAGGAACCTGAT GCTGTGGCACCAGACCCCGATTTCCTGGATA TTATCACCCTGCACAGACCAGCACTGACCAGT CGGAGAGGGACAGTGAGATTTTCAAGGCTG GGACAGAAGGCCACTATGCGAACCCGGAGC GGAAAACAGATCGGCGCTAGGGTGCACTACT ATCATGACATTTCCCGCATCGCCCCCGCTGAT GAGCTGGAAATGCAGCCTCTGCTGAGTCCAT CAAACAATTACAGCTATGACATCTACGCAGA CCTGGATGAGGCCGAAACTGGCTTCATCCAG CCCACCCACACTACCCCTATGAGTCATTCAAG CCTGTCAAGGCAGCTGCCAAGCCTGTCCTCTA GTATGTCAAGCTCCTACGCCAACGTGACCATT CCCTTTTCCACAACTTATTCTGTCCCAATCCAT ACAGGGCCCGACGTGGTCCTGCCTACATCCC CAACTGTGTGGCCCTATGTCCCTCACACCTCC ATCGACACAAAACATTCTATTGTGATCCTGGG AGGCGATTACTATCTGTGGCCTTACACTCACC TGCTGCGGAAGAGGCGCAAAAGAATCCCCTA TTTCTTTACAGATGGCATCGTGGCTCAT MVATRARRRKRASVTQLYSTCKAAG TCPPDVVNKVEGTTLADKILQWSGL GIFLGGLGIGTGSGSGGRTGYIPLGG GGRPGVVDIAPARPPIIIDLWHHTEP SIVNLVEDSSIIQSGSPIPTFTGTDGFE ITSSSTTTPAVLDITPSAGTVHVSSTNI ENPLYIEPPSIEAPQSGEVSDIYLLVHY SGTHGYEEIPMEVFASNVSTGTEPIS STPTPGVSRIAAPRLYSKSYTQVKVT NPDFISKPSTFVTFNNPAFEPIDTSITF EEPDAVAPDPDFLDIITLHRPALTSRR GTVRFSRLGQKATMRTRSGKQIGAR VHYYHDISRIAPADELEMQPLLSPSN NYSYDIYADLDEAETGFIQPTHTTPM SHSSLSRQLPSLSSSMSSSYANVTIPF STTYSVPIHTGPDVVLPTSPTVWPYV PHTSIDTKHSIVILGGDYYLWPYTHLL RKRRKRIPYFFTDGIVAH Page 40 of 48 % Nucleotide Change 28.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV52 E1 ATGGAGGACCCTGAAGGTACAGAGGGCGAA AGGGAGGGATGTACAGGCTGGTTTGAAGTA GAGGCAATAATAGAAAAACAAACAGGAGAT AACATTTCAGAGGACGAGGATGAAAATGCAT ATGATAGTGGAACAGATCTAATAGATTTTATA GATGATTCAAATATAAATAATGAACAGGCAG AACATGAGGCAGCCCGGGCATTGTTTAATGC ACAGGAAGGGGAGGATGATTTACATGCTGT GTCTGCAGTAAAACGAAAGTTTACAAGCAGT CCGGAAAGTGCTGGGCAAGATGGTGTAGAA AAACATGGTAGTCCGCGTGCAAAACACATTT GTGTAAATACAGAGTGTGTTTTACCAAAACG CAAACCATGTCACGTAGAAGACAGCGGCTAT GGCAATAGTGAAGTGGAAGCGCAGCAGATG GCAGACCAGGTAGACGGGCAAAATGGCGAC TGGCAAAGTAACAGTAGTCAATCAAGTGGGG TGGGGGCTAGTAATTCAGATGTAAGTTGTAC TAGTATAGAGGACAATGAGGAAAATAGTAAT AGAACGCTAAAAAGCATACAAAATATTATGT GCGAAAATAGCATAAAAACAACTGTATTATTT AAATTTAAAGAAACATATGGTGTTAGCTTTAT GGAATTAGTAAGACCATTTAAAAGTAATAGA AGTAGTTGTACAGATTGGTGTATTATAGGAA TGGGAGTAACACCATCAGTTGCAGAAGGATT AAAAGTATTAATACAGCCCTATAGCATATATG CCCATTTGCAATGTTTAACATGTGACAGAGGC GTGCTTATACTGCTGCTAATTAGGTTTAAATG TGGAAAAAACAGATTAACAGTGTCCAAACTA ATGTCACAGCTGTTAAATATACCAGAAACAC ATATGGTAATAGAACCACCAAAATTACGAAG TGCTACCTGTGCATTATATTGGTATAGAACAG GTTTGTCTAATATTAGTGAGGTATATGGTACC ACCCCAGAATGGATAGAACAACAAACAGTAT TACAGCATAGCTTTGACAATAGCATATTCGAT TTTGGAGAAATGGTGCAATGGGCATATGATC ATGATATAACAGATGATAGTGACATAGCATA TAAATATGCACAGTTAGCAGATGTAAATAGC AATGCTGCAGCATTCCTAAAAAGCAATTCGC AAGCAAAAATAGTAAAGGACTGTGCAACCAT GTGTAGACATTATAAACGGGCAGAAAGAAA ACATATGAATATTGGACAATGGATACAGTAT AGATGTGATAGAATAGATGATGGTGGAGATT GGAGGCCTATAGTAAGATTTTTAAGATATCA AGACATAGAATTTACAGCCTTTTTAGACGCAT TTAAAAAATTTTTAAAAGGTATACCTAAAAAA AATTGTTTAGTATTATATGGACCTGCAAACAC AGGAAAATCATATTTTGGAATGAGTTTAATTA GGTTCTTAAGTGGATGTGTAATATCCTATGTA AACTCAAAAAGCCATTTTTGGCTACAACCATT AACAGATGCAAAAGTGGGTATGATAGATGAT GTAACACCTATATGTTGGACATATATAGATGA TTATATGAGAAATGCACTGGATGGAAATGAT ATATCAGTAGATGTAAAGCATAGAGCCTTAG TACAAATAAAATGCCCACCATTAATTTTAACA ACAAATACAAATGCAGGAACAGATCCTAGGT GGCCATATTTACATAGTAGATTGGTTGTGTTT CATTTCAAAAACCCATTTCCATTTGATGAAAA TGGCAATCCTATATATGAAATTAACAACGAA AATTGGAAATCCTTTTTCTCAAGGACGTGGTG CAAATTAGATTTAATACAGGAAGAGGACAAG GAAAACGATGGAGTCGATACCGGCACGTTTA AATGCAGTGCAGGAAAAAATACTAGATCTAT ACGAAGCTGA ATGGAAGACCCCGAGGGAACTGAAGGAGAA AGAGAAGGATGCACAGGCTGGTTTGAGGTG GAGGCTATCATTGAAAAGCAGACCGGAGAC AACATTTCAGAGGACGAAGATGAGAATGCTT ACGATAGCGGCACCGACCTGATCGATTTCATT GACGATAGCAACATCAACAATGAGCAGGCA GAACACGAGGCAGCTCGGGCACTGTTCAATG CCCAGGAAGGAGAGGACGATCTGCATGCAG TGTCCGCCGTCAAGAGAAAGTTCACCAGCAG CCCAGAAAGTGCAGGACAGGACGGAGTGGA GAAGCACGGCTCACCCAGAGCTAAACATATC TGCGTGAACACCGAATGTGTCCTGCCAAAGA GGAAACCCTGCCACGTGGAGGACTCCGGATA CGGCAATTCTGAAGTGGAGGCTCAGCAGATG GCAGACCAGGTCGATGGGCAGAACGGAGAT TGGCAGAGCAATTCTAGTCAGTCAAGCGGGG TGGGAGCCAGTAACTCAGACGTCTCATGCAC AAGCATTGAAGATAATGAGGAAAACTCTAAT CGGACTCTGAAAAGTATCCAGAACATTATGT GTGAGAACAGCATCAAGACCACAGTGCTGTT CAAGTTTAAAGAAACCTACGGCGTGAGCTTC ATGGAGCTGGTCCGCCCTTTTAAGTCTAACCG ATCCTCTTGCACTGACTGGTGTATCATTGGAA TGGGAGTGACCCCAAGCGTCGCAGAGGGAC TGAAGGTGCTGATCCAGCCTTACTCCATCTAC GCCCACCTGCAGTGCCTGACTTGTGATAGAG GGGTGCTGATCCTGCTGCTGATTCGGTTTAA GTGCGGAAAAAACAGACTGACCGTGTCTAAA CTGATGAGTCAGCTGCTGAATATCCCCGAAA CACACATGGTCATCGAGCCCCCTAAGCTGCG ATCTGCTACCTGTGCACTGTACTGGTATCGGA CAGGACTGTCCAACATTTCTGAAGTGTACGG CACTACCCCTGAATGGATCGAGCAGCAGACA GTCCTGCAGCACTCATTCGACAATAGCATCTT CGATTTTGGCGAGATGGTGCAGTGGGCTTAT GACCATGATATCACTGACGATTCTGACATTGC ATACAAATATGCCCAGCTGGCTGATGTGAAC AGTAATGCAGCCGCTTTTCTGAAGAGCAACT CCCAGGCAAAGATCGTCAAAGACTGCGCCAC CATGTGTAGGCACTACAAGCGGGCCGAGAG AAAACACATGAATATCGGCCAGTGGATTCAG TATAGGTGCGACCGAATCGACGATGGAGGG GATTGGCGACCAATTGTGCGATTCCTGAGAT ACCAGGACATCGAGTTCACCGCCTTTCTGGAT GCTTTCAAGAAATTTCTGAAAGGCATCCCCAA GAAGAACTGCCTGGTGCTGTACGGACCAGCT AATACAGGCAAGAGTTATTTCGGGATGTCAC TGATCAGGTTTCTGAGCGGCTGTGTGATTTCC TATGTCAACTCTAAAAGTCACTTTTGGCTGCA GCCTCTGACAGACGCCAAAGTGGGGATGATT GACGATGTCACACCAATCTGCTGGACTTACAT TGACGATTATATGCGCAACGCTCTGGACGGA AATGATATCTCTGTGGATGTCAAGCATCGAG CACTGGTGCAGATCAAATGTCCACCCCTGATT CTGACAACTAACACTAATGCAGGCACCGACC CCAGGTGGCCTTACCTGCACAGCCGCCTGGT GGTCTTCCATTTTAAGAACCCTTTCCCATTTGA TGAAAACGGGAACCCCATCTATGAAATTAAC AACGAGAACTGGAAGAGTTTCTTTTCACGCA CTTGGTGCAAACTGGACCTGATTCAGGAGGA AGATAAGGAGAACGACGGCGTGGATACCGG GACATTCAAGTGTAGCGCCGGCAAAAATACC AGAAGCATCAGGTCC MEDPEGTEGEREGCTGWFEVEAIIE KQTGDNISEDEDENAYDSGTDLIDFI DDSNINNEQAEHEAARALFNAQEGE DDLHAVSAVKRKFTSSPESAGQDGV EKHGSPRAKHICVNTECVLPKRKPCH VEDSGYGNSEVEAQQMADQVDGQ NGDWQSNSSQSSGVGASNSDVSCT SIEDNEENSNRTLKSIQNIMCENSIKT TVLFKFKETYGVSFMELVRPFKSNRS SCTDWCIIGMGVTPSVAEGLKVLIQP YSIYAHLQCLTCDRGVLILLLIRFKCGK NRLTVSKLMSQLLNIPETHMVIEPPK LRSATCALYWYRTGLSNISEVYGTTP EWIEQQTVLQHSFDNSIFDFGEMVQ WAYDHDITDDSDIAYKYAQLADVNS NAAAFLKSNSQAKIVKDCATMCRHY KRAERKHMNIGQWIQYRCDRIDDG GDWRPIVRFLRYQDIEFTAFLDAFKK FLKGIPKKNCLVLYGPANTGKSYFGM SLIRFLSGCVISYVNSKSHFWLQPLTD AKVGMIDDVTPICWTYIDDYMRNAL DGNDISVDVKHRALVQIKCPPLILTT NTNAGTDPRWPYLHSRLVVFHFKNP FPFDENGNPIYEINNENWKSFFSRT WCKLDLIQEEDKENDGVDTGTFKCS AGKNTRSIRS Page 41 of 48 % Nucleotide Change 25.5% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV52 E2 ATGGAGTCGATACCGGCACGTTTAAATGCAG TGCAGGAAAAAATACTAGATCTATACGAAGC TGATAGTAATGACCTAAACGCACAAATTGAA CATTGGAAATTGACTCGAATGGAATGTGTTTT GTTTTACAAAGCAAAGGAACTGGGAATAACT CATATAGGCCACCAGGTGGTGCCACCAATGG CAGTGTCTAAGGCAAAGGCCTGCCAAGCTAT TGAACTACAATTGGCATTGGAGGCATTAAAC AAAACACAATATAGCACAGATGGATGGACAT TACAACAAACAAGTCTAGAAATGTGGCGTGC AGAACCACAAAAATACTTTAAAAAACATGGG TATACAATAACAGTGCAATACGATAATGATA AAAACAATACTATGGATTATACAAACTGGAA GGAAATTTATTTACTTGGTGAGTGTGAATGT ACAATTGTAGAAGGACAAGTAGATTACTATG GGTTATATTATTGGTGTGATGGAGAAAAAAT ATATTTTGTAAAATTTAGTAACGATGCAAAGC AATATTGTGTAACAGGAGTATGGGAAGTACA TGTGGGTGGTCAGGTAATTGTTTGTCCTGCAT CTGTATCTAGTAACGAAGTATCCACTACTGAA ACTGCTGTCCACCTATGCACCGAAACCTCCAA GACCTCCGCAGTGTCCGTGGGTGCCAAAGAC ACACACCTACAACCACCACAGAAACGACGAC GACCAGACGTCACAGACTCCAGAAACACCAA GTACCCCAACAACCTTTTGCGGGGACAACAA TCCGTGGACAGTACTACACGGGGACTCGTCA CTGCAACTGAGTGCACAAACAAAGGACGGGT TGCACATACAACTTGTACTGCACCTATAATAC ACCTAAAAGGTGATCCTAATAGTTTAAAATGT TTAAGATATAGGGTAAAAACACATAAAAGTT TGTATGTTCAAATTTCATCTACCTGGCATTGG ACCAGTAATGAATGTACAAATAATAAACTAG GTATTGTAACAATAACGTACAGTGATGAAAC ACAACGTCAACAATTTTTAAAAACTGTTAAAA TACCAAATACTGTGCAAGTTATACAAGGTGTC ATGTCATTGTGA TTGTTTGTCCTGCATCTGTATCTAGTAACGAA GTATCCACTACTGAAACTGCTGTCCACCTATG CACCGAAACCTCCAAGACCTCCGCAGTGTCC GTGGGTGCCAAAGACACACACCTACAACCAC CACAGAAACGACGACGACCAGACGTCACAG ACTCCAGAAACACCAAGTACCCCAACAACCTT TTGCGGGGACAACAATCCGTGGACAGTACTA CACGGGGACTCGTCACTGCAACTGAGTGCAC AAACAAAGGACGGGTTGCACATACAACTTGT ACTGCACCTATAA ATGTTAGGATTATTTGTATTTTGTTTTATTTTG CTTATGGTGTTTTGTGCAGTGCTTAGGCCGCT CTTGCTATCTATATCGGTGTATGCGCAGGTGT TGGTGCTGGTGCTTTTGCTATGGGTATCTATT GGGTCACCATTTAAAGTGTTTTTTTTGTACCT ACTGTTTTTATATTTTCCAATGTTTTGTATTCA CTGTCATGCACAGTATTTGGCACAACTGCAAT AA ATGTTTGAGGATCCAGCAACACGACCCCGGA CCCTGCACGAATTGTGTGAGGTGCTGGAAGA ATCGGTGCATGAAATAAGGCTGCAGTGTGTG CAGTGCAAAAAAGAGCTACAACGAAGAGAG GTATACAAGTTTCTATTTACAGATTTACGAAT AGTATATAGAGACAATAATCCATATGGCGTG TGTATTATGTGCCTACGCTTTTTATCTAAGAT AAGTGAATATAGGCATTATCAATATTCACTGT ATGGGAAAACATTAGAAGAGAGGGTAAAAA AACCATTAAGTGAAATAACTATTAGATGTATA ATTTGTCAAACGCCATTATGTCCTGAAGAAAA AGAAAGACATGTTAATGCAAACAAGCGATTT CATAATATTATGGGTCGTTGGACAGGGCGCT GTTCAGAGTGTTGGAGACCCCGACCTGTGAC CCAAGTGTAA ATGGAAAGTATCCCCGCAAGACTGAACGCCG TGCAGGAAAAAATTCTGGACCTGTATGAAGC CGACTCAAATGATCTGAACGCCCAGATCGAG CACTGGAAGCTGACTCGCATGGAATGCGTGC TGTTCTATAAGGCCAAAGAGCTGGGAATCAC ACACATTGGCCATCAGGTGGTCCCCCCTATG GCAGTGAGCAAGGCCAAGGCCTGCCAGGCC ATTGAGCTGCAGCTGGCACTGGAAGCCCTGA ACAAAACTCAGTACTCCACCGACGGCTGGAC ACTGCAGCAGACTTCTCTGGAGATGTGGCGA GCTGAACCACAGAAGTACTTTAAGAAACACG GGTATACCATCACAGTGCAGTACGACAACGA TAAGAACAACACAATGGACTACACAAACTGG AAGGAAATCTACCTGCTGGGGGAGTGCGAAT GTACCATTGTGGAAGGGCAGGTGGACTACTA TGGACTGTACTATTGGTGCGATGGGGAGAAA ATCTACTTCGTGAAGTTCAGCAACGACGCCA AGCAGTACTGCGTGACCGGAGTCTGGGAAG TGCATGTCGGCGGGCAGGTCATCGTCTGTCC CGCTTCAGTGAGCTCCAATGAGGTCAGCACC ACAGAAACAGCAGTGCACCTGTGTACTGAGA CCTCAAAGACTAGCGCTGTGTCCGTCGGCGC AAAAGATACCCATCTGCAGCCACCCCAGAAG CGGAGAAGGCCCGACGTGACAGATAGCCGG AATACTAAATATCCTAACAATCTGCTGAGAG GCCAGCAGTCTGTGGACAGTACTACCAGGGG GCTGGTCACAGCCACTGAGTGCACAAACAAG GGAAGGGTGGCCCACACAACTTGTACTGCTC CTATCATTCATCTGAAGGGCGATCCAAATAGT CTGAAATGCCTGCGCTATCGAGTGAAGACCC ACAAATCACTGTACGTCCAGATCAGCAGCAC CTGGCATTGGACCAGCAACGAGTGTACCAAC AATAAGCTGGGAATCGTGACCATTACATACT CCGACGAAACACAGCGGCAGCAGTTCCTGAA GACCGTGAAAATCCCTAATACAGTGCAGGTC ATTCAGGGCGTCATGTCTCTG ATGTTCGTCCTGCACCTGTACCTGGTCACTAA ATACCCACTGCTGAAACTGCTGTCAACTTACG CACCTAAACCTCCTCGCCCACCCCAGTGCCCC TGGGTGCCTAAGACTCACACCTACAACCACC ATCGGAATGACGATGACCAGACATCTCAGAC TCCAGAGACCCCCAGTACACCTACCACATTCT GTGGCGATAACAATCCCTGGACTGTGCTGCA TGGGGACAGCTCCCTGCAGCTGTCCGCACAG ACAAAAGATGGCCTGCACATCCAGCTGGTCC TGCATCTG ATGCTGGGACTGTTTGTGTTCTGCTTTATTCT GCTGATGGTGTTTTGTGCCGTGCTGAGACCC CTGCTGCTGAGTATTAGCGTGTATGCCCAGG TGCTGGTCCTGGTGCTGCTGCTGTGGGTCAG CATCGGCTCCCCCTTTAAGGTGTTCTTTCTGT ACCTGCTGTTCCTGTATTTTCCTATGTTCTGCA TTCACTGTCATGCCCAGTACCTGGCTCAGCTG CAG ATGTTTGAAGACCCCGCTACAAGACCAAGAA CCCTGCATGAACTGTGCGAAGTGCTGGAGGA ATCCGTCCACGAAATCAGACTGCAGTGCGTG CAGTGTAAGAAAGAGCTGCAGCGGAGAGAA GTCTACAAGTTCCTGTTTACAGACCTGCGAAT CGTGTACCGGGATAACAATCCTTATGGAGTC TGCATCATGTGTCTGAGGTTCCTGAGCAAGA TTTCCGAGTACCGCCACTACCAGTATTCTCTG TATGGCAAAACCCTGGAGGAACGGGTGAAG AAACCCCTGAGTGAGATCACCATTAGATGCA TCATTTGTCAGACACCACTGTGCCCCGAGGA AAAGGAACGCCACGTGAACGCCAACAAGCG ATTTCATAACATTATGGGCAGATGGACTGGG AGGTGCTCCGAATGTTGGAGGCCCCGCCCTG T MESIPARLNAVQEKILDLYEADSNDL NAQIEHWKLTRMECVLFYKAKELGIT HIGHQVVPPMAVSKAKACQAIELQL ALEALNKTQYSTDGWTLQQTSLEM WRAEPQKYFKKHGYTITVQYDNDK NNTMDYTNWKEIYLLGECECTIVEG QVDYYGLYYWCDGEKIYFVKFSNDA KQYCVTGVWEVHVGGQVIVCPASV SSNEVSTTETAVHLCTETSKTSAVSV GAKDTHLQPPQKRRRPDVTDSRNTK YPNNLLRGQQSVDSTTRGLVTATEC TNKGRVAHTTCTAPIIHLKGDPNSLK CLRYRVKTHKSLYVQISSTWHWTSN ECTNNKLGIVTITYSDETQRQQFLKT VKIPNTVQVIQGVMSL HPV52 E4 HPV52 E5 HPV52 E6 Page 42 of 48 % Nucleotide Change 25.7% LFVLHLYLVTKYPLLKLLSTYAPKPPRP PQCPWVPKTHTYNHHRNDDDQTS QTPETPSTPTTFCGDNNPWTVLHG DSSLQLSAQTKDGLHIQLVLHL 23.1% MLGLFVFCFILLMVFCAVLRPLLLSIS VYAQVLVLVLLLWVSIGSPFKVFFLYL LFLYFPMFCIHCHAQYLAQLQ 23.0% MFEDPATRPRTLHELCEVLEESVHEI RLQCVQCKKELQRREVYKFLFTDLRI VYRDNNPYGVCIMCLRFLSKISEYRH YQYSLYGKTLEERVKKPLSEITIRCIICQ TPLCPEEKERHVNANKRFHNIMGR WTGRCSECWRPRPVTQV 27.5% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV52 E7 ATGCGTGGAGACAAAGCAACTATAAAAGATT ATATATTAGATCTGCAACCTGAAACAACTGAC CTACACTGCTATGAGCAATTAGGTGACAGCT CAGATGAGGAGGATACAGATGGTGTGGACC GGCCAGATGGACAAGCAGAACAAGCCACAA GCAATTACTACATTGTGACATATTGTCACAGT TGTGATAGCACACTACGGCTATGCATTCATAG CACTGCGACGGACCTTCGTACTCTACAGCAA ATGCTGTTGGGCACATTACAAGTTGTGTGCC CCGGCTGTGCACGGCTATAA ATGTCCGTGTGGCGGCCTAGTGAGGCCACTG TGTACCTGCCTCCTGTACCTGTCTCTAAGGTT GTAAGCACTGATGAGTATGTGTCTCGCACAA GCATCTATTATTATGCAGGCAGTTCTCGATTA CTAACAGTAGGACATCCCTATTTTTCTATTAA AAACACCAGTAGTGGTAATGGTAAAAAAGTT TTAGTTCCCAAGGTGTCTGGCCTGCAATACA GGGTATTTAGAATTAAATTGCCGGACCCTAAT AAATTTGGTTTTCCAGATACATCTTTTTATAAC CCAGAAACCCAAAGGTTGGTGTGGGCCTGTA CAGGCTTGGAAATTGGTAGGGGACAGCCTTT AGGTGTGGGTATTAGTGGGCATCCTTTATTA AACAAGTTTGATGATACTGAAACCAGTAACA AATATGCTGGTAAACCTGGTATAGATAATAG GGAATGTTTATCTATGGATTATAAGCAGACTC AGTTATGCATTTTAGGATGCAAACCTCCTATA GGTGAACATTGGGGTAAGGGAACCCCTTGTA ATAATAATTCAGGAAATCCTGGGGATTGTCCT CCCCTACAGCTCATTAACAGTGTAATACAGGA TGGGGACATGGTAGATACAGGATTTGGTTGC ATGGATTTTAATACCTTGCAAGCTAGTAAAAG TGATGTGCCCATTGATATATGTAGCAGTGTAT GTAAGTATCCAGATTATTTGCAAATGGCTAGC GAGCCATATGGTGACAGTTTGTTCTTTTTTCT TAGACGTGAGCAAATGTTTGTTAGACACTTTT TTAATAGGGCCGGTACCTTAGGTGACCCTGT GCCAGGTGATTTATATATACAAGGGTCTAACT CTGGCAATACTGCCACTGTACAAAGCAGTGC TTTTTTTCCTACTCCTAGTGGTTCTATGGTAAC CTCAGAATCCCAATTATTTAATAAACCGTACT GGTTACAACGTGCGCAGGGCCACAATAATGG CATATGTTGGGGCAATCAGTTGTTTGTCACA GTTGTGGATACCACTCGTAGCACTAACATGA CTTTATGTGCTGAGGTTAAAAAGGAAAGCAC ATATAAAAATGAAAATTTTAAGGAATACCTTC GTCATGGCGAGGAATTTGATTTACAATTTATT TTTCAATTGTGCAAAATTACATTAACAGCTGA TGTTATGACATACATTCATAAGATGGATGCCA CTATTTTAGAGGACTGGCAATTTGGCCTTACC CCACCACCGTCTGCATCTTTGGAGGACACATA CAGATTTGTCACTTCTACTGCTATAACTTGTC AAAAAAACACACCACCTAAAGGAAAGGAAG ATCCTTTAAAGGACTATATGTTTTGGGAGGT GGATTTAAAAGAAAAGTTTTCTGCAGATTTA GATCAGTTTCCTTTAGGTAGGAAGTTTTTGTT ACAGGCAGGGCTACAGGCTAGGCCCAAACTA AAACGCCCTGCATCATCGGCCCCACGTACCTC CACAAAGAAGAAAAAGGTTAAAAGGTAA ATGAGAGGAGACAAAGCCACCATCAAGGATT ACATTCTGGACCTGCAGCCTGAGACAACTGA CCTGCATTGCTATGAACAGCTGGGGGACAGC TCCGATGAGGAAGACACCGATGGAGTGGAC AGGCCAGATGGACAGGCAGAGCAGGCTACT AGCAACTACTATATCGTCACCTACTGCCACTC TTGTGACAGTACACTGCGGCTGTGCATTCATT CTACCGCAACAGATCTGAGAACACTGCAGCA GATGCTGCTGGGAACTCTGCAGGTGGTCTGC CCTGGCTGTGCCCGGCTG ATGGTCCAGATCCTGTTTTATATCCTGGTCAT TTTCTATTATGTCGCCGGGGTCAACGTCTTTC ACATTTTCCTGCAGATGAGCGTCTGGAGGCC TAGCGAGGCCACCGTGTATCTGCCCCCTGTG CCAGTCTCTAAGGTGGTCAGTACAGACGAAT ACGTGAGCCGGACTTCCATCTACTATTACGCT GGAAGCTCCAGACTGCTGACAGTGGGCCACC CCTACTTTTCTATTAAGAATACTTCTAGTGGC AACGGGAAGAAAGTGCTGGTCCCTAAAGTG AGTGGACTGCAGTATAGGGTCTTTCGCATCA AGCTGCCAGACCCCAACAAGTTTGGCTTCCCA GATACCAGCTTCTACAACCCCGAGACACAGA GGCTGGTGTGGGCTTGCACCGGCCTGGAAAT CGGACGAGGACAGCCACTGGGGGTCGGAAT TAGTGGGCACCCTCTGCTGAATAAGTTCGAC GATACTGAGACCTCAAACAAGTATGCCGGGA AACCTGGAATTGACAATCGCGAATGTCTGAG CATGGACTACAAACAGACCCAGCTGTGCATC CTGGGCTGTAAGCCACCCATTGGGGAGCATT GGGGCAAAGGGACACCTTGCAACAATAACA GCGGCAATCCAGGGGACTGTCCTCCACTGCA GCTGATCAACTCCGTGATTCAGGACGGCGAT ATGGTGGACACCGGATTTGGCTGCATGGATT TCAACACACTGCAGGCTAGCAAGTCCGACGT GCCCATCGATATTTGCTCAAGCGTCTGTAAAT ATCCAGACTACCTGCAGATGGCATCAGAGCC CTATGGCGATAGCCTGTTCTTTTTCCTGCGGA GAGAACAGATGTTCGTGCGACACTTTTTCAAT CGAGCAGGAACCCTGGGCGACCCTGTCCCAG GGGATCTGTACATCCAGGGGTCTAATAGTGG AAACACAGCTACTGTGCAGTCCTCTGCATTTT TCCCCACTCCTTCAGGAAGCATGGTCACCTCC GAGTCTCAGCTGTTTAACAAGCCCTATTGGCT GCAGCGAGCACAGGGCCATAATAACGGGAT TTGCTGGGGAAATCAGCTGTTCGTGACTGTG GTCGATACCACACGCTCCACCAACATGACACT GTGTGCCGAGGTGAAGAAAGAATCTACATAC AAGAACGAGAACTTCAAGGAATACCTGAGGC ACGGCGAGGAGTTCGACCTGCAGTTTATCTT CCAGCTGTGCAAGATTACCCTGACAGCCGAT GTGATGACATACATCCATAAAATGGACGCTA CTATTCTGGAGGATTGGCAGTTTGGCCTGAC TCCCCCTCCAAGTGCATCACTGGAAGACACCT ATCGGTTCGTGACTTCTACCGCCATCACTTGT CAGAAGAATACCCCCCCTAAGGGGAAAGAG GACCCACTGAAAGATTACATGTTTTGGGAGG TGGATCTGAAGGAAAAATTCAGCGCCGACCT GGATCAGTTTCCCCTGGGGAGAAAGTTCCTG CTGCAGGCAGGACTGCAGGCCAGACCAAAG CTGAAAAGGCCCGCCAGTTCAGCTCCTCGCA CAAGCACTAAGAAAAAGAAAGTGAAGCGA MRGDKATIKDYILDLQPETTDLHCYE QLGDSSDEEDTDGVDRPDGQAEQA TSNYYIVTYCHSCDSTLRLCIHSTATD LRTLQQMLLGTLQVVCPGCARL HPV52 L1 Page 43 of 48 MSVWRPSEATVYLPPVPVSKVVSTD EYVSRTSIYYYAGSSRLLTVGHPYFSIK NTSSGNGKKVLVPKVSGLQYRVFRIK LPDPNKFGFPDTSFYNPETQRLVWA CTGLEIGRGQPLGVGISGHPLLNKFD DTETSNKYAGKPGIDNRECLSMDYK QTQLCILGCKPPIGEHWGKGTPCNN NSGNPGDCPPLQLINSVIQDGDMV DTGFGCMDFNTLQASKSDVPIDICSS VCKYPDYLQMASEPYGDSLFFFLRRE QMFVRHFFNRAGTLGDPVPGDLYIQ GSNSGNTATVQSSAFFPTPSGSMVT SESQLFNKPYWLQRAQGHNNGICW GNQLFVTVVDTTRSTNMTLCAEVKK ESTYKNENFKEYLRHGEEFDLQFIFQL CKITLTADVMTYIHKMDATILEDWQ FGLTPPPSASLEDTYRFVTSTAITCQK NTPPKGKEDPLKDYMFWEVDLKEKF SADLDQFPLGRKFLLQAGLQARPKLK RPASSAPRTSTKKKKVKR % Nucleotide Change 23.7% 31.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV52 L2 ATGAGATACAGACGGTCTACACGGCACAAAC GTGCTTCTGCAACACAGCTATATCAAACATGC AAAGCCTCTGGCACCTGCCCCCCCGATGTTAT TCCTAAAGTGGAAGGCACAACTATTGCAGAT CAACTTTTAAAATATGGCAGCCTAGGGGTGT TTTTTGGAGGTTTGGGTATAGGTACAGGTGC AGGCTCTGGTGGTAGGGCAGGCTATGTGCCA TTGTCCACTCGTCCTCCCACTAGTAGTATTAC CACGTCCACCATTCGTCCCCCTGTAACTGTAG AACCCATTGGTCCCTTAGAACCATCTATAGTT TCTATGATAGAAGAAACAACATTTATTGAGTC TGGCGCACCTGCTCCATCTATTCCATCAGCAA CAGGGTTTGATGTTACAACATCTGCAAATAAT ACTCCTGCAATAATTAATGTAACATCTATAGG TGAATCATCTGTACAATCAGTTTCTACACATT TAAATCCTACATTCACTGAACCATCTATAATA CAGCCCCCGGCACCTGCAGAAGCATCTGGTC ATGTATTGTTTTCTAGTCCAACTATTAGTACA CACACCTATGAAGAAATCCCTATGGATACATT TGTTACCTCTACTGACAGCAGCAGTGTAACAA GTAGTACACCTATTCCAGGGTCTCGCCCTACG ACACGCCTTGGTTTATATAGCCGTGCCACACA ACAGGTTAAGGTAGTCGACCCTGCTTTTATGT CATCACCACAGAAATTAGTAACATATAACAAT CCTGTTTTTGAGGGCGTTGATACAGATGAAA CTATAATTTTTGATCGTTCACAACTTTTACCTG CACCGGATCCTGATTTTTTAGACATTATAGCT TTGCATAGGCCTGCATTAACCTCTCGAAGAG GTACTGTTAGGTTTAGCAGGCTTGGTAATAA GGCCACCCTACGTACACGTAGTGGAAAACAA ATTGGGGCACGGGTACATTATTATCATGATA TTAGTCCTATCCAGCCTGCTGAAGTTCAGGAA GACATAGAATTGCAACCTTTATTACCACAGTC TGTGTCCCCTTACACTATTAATGATGGTTTGT ATGATGTGTATGCAGATTCTTTGCAGCAACCC ACGTTTCACTTACCTTCCACACTTTCTACCCAT AATAATACTTTCACTGTACCTATTAATAGTGG TATTGACTTTGTATATCAACCCACTATGTCCAT TGAGTCAGGTCCTGACATTCCATTACCTTCGT TACCCACACATACTCCTTTTGTTCCTATAGCCC CTACAGCTCCATCTACATCTATTATTGTTGAT GGTACAGATTTTATTTTACATCCTAGTTATTTT TTACTACGTCGCAGGCGTAAACGTTTTCCATA TTTTTTTACAGATGTCCGTGTGGCGGCCTAG ATGAGGTATCGGCGAAGCACTCGGCATAAAA GGGCATCCGCAACCCAGCTGTATCAGACTTG TAAGGCATCCGGCACTTGTCCCCCAGATGTG ATCCCCAAGGTCGAAGGCACCACAATTGCTG ACCAGCTGCTGAAATACGGAAGCCTGGGCGT GTTCTTTGGAGGACTGGGAATCGGAACCGGA GCTGGGTCAGGAGGCAGGGCAGGATATGTG CCTCTGAGCACACGCCCCCCTACTAGCTCCAT CACTACCTCTACAATTAGGCCACCCGTGACTG TCGAACCCATCGGCCCTCTGGAGCCATCAATC GTGAGCATGATTGAGGAAACAACTTTCATCG AAAGCGGAGCACCAGCACCTTCCATTCCATCT GCCACCGGATTTGATGTGACCACATCCGCCA ACAATACCCCTGCTATCATTAACGTCACATCT ATCGGGGAGTCTAGTGTGCAGAGTGTCTCAA CCCACCTGAATCCAACATTCACTGAACCCAGT ATCATTCAGCCTCCAGCTCCCGCAGAGGCCTC AGGACACGTGCTGTTCTCAAGCCCAACTATCA GCACCCATACATACGAGGAAATTCCTATGGA CACCTTTGTGACTAGCACCGACTCCTCTAGTG TCACTTCAAGCACCCCAATCCCAGGCTCCCGG CCAACTACCAGACTGGGGCTGTACTCTAGGG CCACCCAGCAGGTGAAGGTGGTGGACCCCGC TTTTATGTCCTCTCCTCAGAAACTGGTGACAT ATAACAATCCCGTGTTCGAAGGCGTGGACAC AGATGAGACTATCATTTTTGATCGGTCCCAGC TGCTGCCTGCACCAGACCCCGATTTCCTGGAC ATCATTGCACTGCATAGACCCGCCCTGACTTC TCGGAGAGGCACCGTGCGGTTCAGCCGGCT GGGAAACAAGGCAACCCTGAGAACAAGGAG TGGGAAACAGATCGGAGCCCGCGTGCACTAC TATCATGATATCAGCCCCATTCAGCCTGCTGA GGTGCAGGAAGACATCGAGCTGCAGCCACT GCTGCCACAGAGCGTGTCCCCTTACACAATTA ACGACGGCCTGTACGATGTCTATGCAGACTC TCTGCAGCAGCCTACTTTCCACCTGCCAAGTA CTCTGTCAACCCATAACAATACATTCACTGTG CCAATCAATAGCGGCATTGATTTTGTCTATCA GCCAACCATGAGCATCGAGTCCGGGCCCGAC ATTCCTCTGCCATCCCTGCCTACCCACACACCT TTCGTGCCAATCGCTCCCACCGCACCTTCTAC AAGTATCATTGTGGACGGGACCGATTTCATT CTGCATCCTAGCTACTTTCTGCTGAGGCGCCG ACGGAAGCGATTTCCATATTTCTTTACAGATG TGCGCGTCGCCGCT MRYRRSTRHKRASATQLYQTCKASG TCPPDVIPKVEGTTIADQLLKYGSLGV FFGGLGIGTGAGSGGRAGYVPLSTR PPTSSITTSTIRPPVTVEPIGPLEPSIVS MIEETTFIESGAPAPSIPSATGFDVTT SANNTPAIINVTSIGESSVQSVSTHLN PTFTEPSIIQPPAPAEASGHVLFSSPTI STHTYEEIPMDTFVTSTDSSSVTSSTP IPGSRPTTRLGLYSRATQQVKVVDPA FMSSPQKLVTYNNPVFEGVDTDETII FDRSQLLPAPDPDFLDIIALHRPALTS RRGTVRFSRLGNKATLRTRSGKQIGA RVHYYHDISPIQPAEVQEDIELQPLLP QSVSPYTINDGLYDVYADSLQQPTFH LPSTLSTHNNTFTVPINSGIDFVYQPT MSIESGPDIPLPSLPTHTPFVPIAPTA PSTSIIVDGTDFILHPSYFLLRRRRKRF PYFFTDVRVAA Page 44 of 48 % Nucleotide Change 27.8% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV58 E1 ATGGATGACCCTGAAGGTACAAACGGGGTA GGGGCGGGCTGTACTGGCTGGTTTGAGGTA GAAGCGGTAATAGAACGAAGAACAGGAGAT AATATTTCAGATGATGAGGACGAAACAGCAG ACGATAGTGGTACAGATTTAATAGAGTTTAT AGATGATTCAGTACAAAGTACTACACAGGCA GAAGCAGAGGCAGCCCGAGCGTTGTTTAATG TACAGGAAGGGGTGGACGATATAAATGCTGT GTGTGCACTAAAACGAAAGTTTGCAGCATGC TCAGAAAGTGCTGTAGAGGACTGTGTGGACC GGGCTGCAAATGTGTGTGTATCGTGGAAATA TAAAAATAAAGAATGCACACACAGAAAACGA AAAATTATTGAGCTAGAAGACAGCGGATATG GCAATACTGAAGTGGAAACTGAGCAGATGG CACACCAGGTAGAAAGCCAAAATGGCGACG CAGACTTAAATGACTCGGAGTCTAGTGGGGT GGGGGCTAGTTCAGATGTAAGCAGTGAAAC GGATGTAGACAGTTGTAATACTGTTCCATTAC AAAATATTAGTAATATTCTACATAACAGTAAT ACTAAAGCAACGCTATTATATAAATTCAAAGA AGCTTATGGAGTAAGTTTTATGGAATTAGTTA GACCATTTAAAAGTGATAAAACAAGCTGTAC AGATTGGTGTATAACAGGGTATGGAATAAGT CCCTCCGTAGCAGAAAGTTTAAAAGTACTAAT TAAACAGCACAGTATATATACACACCTACAAT GTTTAACGTGTGACAGAGGAATTATATTATTA TTGTTAATTAGATTTAAATGTAGCAAAAATAG ATTAACTGTGGCAAAATTAATGAGTAATTTAC TATCAATTCCTGAAACATGTATGATTATCGAG CCACCAAAATTACGAAGTCAAGCATGTGCCTT ATATTGGTTTAGAACAGCAATGTCAAATATAA GTGATGTGCAAGGGACAACACCAGAATGGA TAGATAGATTAACAGTGTTACAGCATAGCTTT AATGATGATATATTTGATTTAAGTGAAATGAT ACAATGGGCATATGATAATGACATTACAGAT GATAGTGACATTGCATATAAATATGCACAGTT AGCAGATGTTAATAGTAATGCAGCAGCATTT TTAAGAAGCAATGCACAAGCAAAAATAGTAA AAGACTGTGGCGTTATGTGCAGACATTATAA AAGAGCAGAAAAGCGTGGTATGACAATGGG ACAATGGATACAAAGTAGGTGTGAAAAAACA AATGATGGAGGTAATTGGAGACCAATAGTAC AATTTTTAAGATATCAAAATATTGAATTTACA GCATTTTTAGTTGCATTTAAACAGTTTTTACA AGGTGTACCAAAAAAAAGTTGTATGTTACTG TGTGGCCCAGCAAATACAGGGAAATCATATT TTGGAATGAGTTTAATACATTTTTTAAAAGGA TGCATTATTTCATATGTAAATTCCAAAAGTCA TTTTTGGTTGCAGCCATTATCAGATGCTAAAC TAGGTATGATAGATGATGTAACAGCCATAAG CTGGACATATATAGATGATTATATGAGAAAT GCATTAGATGGTAACGACATTTCAATAGATG TAAAACATAGGGCATTAGTACAATTAAAATG TCCACCATTAATAATTACCTCAAATACAAATG CAGGCAAAGATTCACGATGGCCATATTTGCA CAGTAGACTAACAGTATTTGAATTTAACAATC CATTTCCATTTGATGCAAATGGTAATCCAGTG TATAAAATAAATGATGAAAATTGGAAATCCTT TTTCTCAAGGACGTGGTGCAAATTAGGCTTA ATAGAGGAAGAGGACAAGGAAAACGATGGA GGAAATATCAGCACGTTTAAGTGCAGTGCAG GACAAAATCCTAGACATATACGAAGCTGA ATGGACGATCCTGAGGGAACTAACGGGGTG GGGGCTGGCTGCACTGGCTGGTTTGAGGTG GAGGCTGTCATTGAAAGAAGAACTGGCGAC AACATCTCCGACGATGAAGATGAGACTGCTG ACGATTCTGGGACCGACCTGATCGAGTTCAT TGACGATTCTGTGCAGAGTACCACACAGGCA GAAGCTGAGGCAGCTCGCGCCCTGTTCAACG TGCAGGAAGGAGTGGACGATATCAATGCCGT GTGTGCTCTGAAGAGGAAATTTGCAGCCTGC TCCGAGTCTGCTGTGGAAGACTGTGTCGATC GCGCTGCAAACGTGTGCGTCTCCTGGAAGTA CAAAAATAAGGAGTGCACCCACCGGAAAAG AAAGATCATTGAACTGGAGGATTCTGGGTAT GGAAACACAGAAGTGGAGACTGAACAGATG GCACATCAGGTCGAGAGTCAGAACGGCGAC GCCGATCTGAATGACTCAGAAAGCTCCGGCG TGGGGGCCTCTAGTGATGTCTCAAGCGAGAC CGACGTGGATTCCTGTAATACAGTCCCTCTGC AGAACATCTCCAATATTCTGCACAACTCTAAT ACAAAGGCCACTCTGCTGTACAAATTCAAGG AGGCTTATGGCGTGTCTTTCATGGAACTGGT CAGACCCTTCAAGAGTGACAAGACCTCATGC ACAGATTGGTGTATCACAGGATACGGCATTA GTCCCTCAGTGGCCGAGTCTCTGAAAGTCCT GATCAAGCAGCACAGTATCTACACACATCTG CAGTGCCTGACTTGTGACAGGGGGATCATTC TGCTGCTGCTGATCAGGTTCAAATGCAGCAA GAACCGCCTGACAGTGGCCAAACTGATGAGC AATCTGCTGTCCATTCCCGAGACTTGTATGAT CATTGAACCACCTAAGCTGCGCAGCCAGGCA TGCGCACTGTACTGGTTTCGAACCGCCATGTC AAACATCAGCGACGTGCAGGGCACTACCCCT GAGTGGATTGATCGGCTGACAGTCCTGCAGC ACTCATTCAACGACGATATCTTTGACCTGAGC GAAATGATTCAGTGGGCCTACGACAATGATA TCACCGACGATAGCGACATTGCTTACAAATAT GCACAGCTGGCCGATGTGAACAGCAATGCCG CTGCATTCCTGCGATCCAACGCTCAGGCAAA AATCGTGAAGGACTGCGGCGTCATGTGCCGG CACTACAAACGGGCCGAGAAGAGAGGGATG ACTATGGGACAGTGGATTCAGAGCCGGTGC GAAAAGACCAACGATGGCGGGAATTGGCGA CCAATCGTGCAGTTTCTGCGGTATCAGAATAT TGAGTTCACAGCTTTTCTGGTGGCATTCAAAC AGTTTCTGCAGGGCGTCCCCAAGAAATCCTG CATGCTGCTGTGTGGCCCTGCCAACACTGGG AAGTCTTACTTCGGAATGAGTCTGATCCACTT TCTGAAAGGATGTATCATTAGCTATGTGAATA GCAAGTCCCATTTCTGGCTGCAGCCCCTGTCC GACGCTAAGCTGGGCATGATCGACGATGTGA CCGCAATCTCTTGGACATACATTGACGATTAT ATGCGGAACGCACTGGACGGGAATGATATCA GTATTGACGTGAAACACAGAGCCCTGGTCCA GCTGAAGTGCCCACCCCTGATCATTACTAGCA ACACCAATGCTGGAAAGGATAGTAGATGGCC TTACCTGCATTCAAGGCTGACCGTGTTCGAGT TTAACAATCCTTTCCCATTTGACGCAAACGGC AACCCAGTGTACAAAATCAACGATGAAAACT GGAAGAGCTTCTTCAGCCGGACTTGGTGTAA ACTGGGCCTGATCGAGGAAGAGGACAAGGA GAACGATGGAGGCAATATTTCAACCTTTAAG TGCAGCGCAGGACAGAACCCAAGGCACATCC GCAGC MDDPEGTNGVGAGCTGWFEVEAVI ERRTGDNISDDEDETADDSGTDLIEFI DDSVQSTTQAEAEAARALFNVQEG VDDINAVCALKRKFAACSESAVEDCV DRAANVCVSWKYKNKECTHRKRKII ELEDSGYGNTEVETEQMAHQVESQ NGDADLNDSESSGVGASSDVSSETD VDSCNTVPLQNISNILHNSNTKATLL YKFKEAYGVSFMELVRPFKSDKTSCT DWCITGYGISPSVAESLKVLIKQHSIY THLQCLTCDRGIILLLLIRFKCSKNRLT VAKLMSNLLSIPETCMIIEPPKLRSQA CALYWFRTAMSNISDVQGTTPEWID RLTVLQHSFNDDIFDLSEMIQWAYD NDITDDSDIAYKYAQLADVNSNAAA FLRSNAQAKIVKDCGVMCRHYKRAE KRGMTMGQWIQSRCEKTNDGGN WRPIVQFLRYQNIEFTAFLVAFKQFL QGVPKKSCMLLCGPANTGKSYFGM SLIHFLKGCIISYVNSKSHFWLQPLSD AKLGMIDDVTAISWTYIDDYMRNAL DGNDISIDVKHRALVQLKCPPLIITSN TNAGKDSRWPYLHSRLTVFEFNNPF PFDANGNPVYKINDENWKSFFSRT WCKLGLIEEEDKENDGGNISTFKCSA GQNPRHIRS Page 45 of 48 % Nucleotide Change 26.5% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV58 E2 ATGGAGGAAATATCAGCACGTTTAAGTGCAG TGCAGGACAAAATCCTAGACATATACGAAGC TGATAAAAATGATTTAACATCACAAATTGAAC ATTGGAAACTAATACGCATGGAGTGTGCTAT AATGTATACAGCCAGACAAATGGGAATATCA CATTTGTGCCACCAGGTGGTGCCGTCATTGG TAGCATCAAAGACTAAAGCGTTTCAAGTAATT GAACTGCAAATGGCATTAGAGACATTAAATG CATCACCATATAAAACAGATGAATGGACATT GCAACAAACAAGCTTAGAAGTGTGGTTATCA GAGCCACAAAAATGCTTTAAAAAAAAAGGCA TAACAGTAACTGTACAATATGACAATGATAA AGCAAACACAATGGATTATACAAATTGGAGT GAAATATATATTATTGAGGAAACAACATGTA CTTTGGTAGCAGGAGAAGTTGACTATGTGGG GTTGTATTATATACATGGCAATGAAAAGACG TATTTTAAATATTTTAAAGAGGATGCAAAAAA GTACTCTAAAACACAATTATGGGAGGTACAT GTGGGTAGTCGGGTAATTGTATGTCCTACAT CTATACCTAGTGATCAAATATCCACTACTGAA ACTGCTGACCCAAAGACCACCGAGGCCACCA ACAACGAAAGTACACAGGGGACAAAGCGAC GACGACTCGATTTACCAGACTCCAGAGACAA CACCCAGTACTCCACAAAGTATACAGACTGC GCCGTGGACAGTAGACCACGAGGAGGAGGA CTACACAGTACAACTAACTGTACATACAAAG GGCGGAACGTGTGTAGTTCTAAAGTTTCACC TATCGTGCATTTAAAAGGTGACCCAAATAGTT TAAAATGTTTAAGATATAGATTAAAACCATTT AAAGACTTATACTGTAATATGTCATCCACATG GCATTGGACCAGTGATGACAAAGGTGACAAA GTAGGAATTGTTACTGTAACATACACAACGG AAACACAACGACAACTGTTTTTAAACACTGTT AAAATACCACCCACTGTGCAAATAAGTACTG GTGTTATGTCATTGTAA TTGTATGTCCTACATCTATACCTAGTGATCAA ATATCCACTACTGAAACTGCTGACCCAAAGAC CACCGAGGCCACCAACAACGAAAGTACACAG GGGACAAAGCGACGACGACTCGATTTACCAG ACTCCAGAGACAACACCCAGTACTCCACAAA GTATACAGACTGCGCCGTGGACAGTAGACCA CGAGGAGGAGGACTACACAGTACAACTAACT GTACATACAAAGGGCGGAACGTGTGTAGTTC TAAAGTTTCACCTATCGTGCATTTAA ATGATATTACCTATTTTTGTTGTTTGTTTTATA CTGTTTTTATGCTTGTGCATTTTTTTGCGGCCA TTGGTGCTATCTATTTCTATATATGCTTGGTTG CTGGTGTTGGTGTTGCTGCTTTGGGTGTCTGT GGGGTCGGCTCTACGAATTTTTTTCTGTTACT TAATATTTTTATATATACCAATGATGTGTATTA ATTTTCATGCACAATACTTAACCCAACAAGAC TAA ATGTTCCAGGACGCAGAGGAGAAACCACGG ACATTGCATGATTTGTGTCAGGCGTTGGAGA CATCTGTGCATGAAATCGAATTGAAATGCGTT GAATGCAAAAAGACTTTGCAGCGATCTGAGG TATATGACTTTGTATTTGCAGATTTAAGAATA GTGTATAGAGATGGAAATCCATTTGCAGTAT GTAAAGTGTGCTTACGATTGCTATCTAAAATA AGTGAGTATAGACATTATAATTATTCGCTATA TGGAGACACATTAGAACAAACACTAAAAAAG TGTTTAAATGAAATATTAATTAGATGTATTAT TTGTCAAAGACCATTGTGTCCACAAGAAAAA AAAAGGCATGTGGATTTAAACAAAAGGTTTC ATAATATTTCGGGTCGTTGGACAGGGCGCTG TGCAGTGTGTTGGAGACCCCGACGTAGACAA ACACAAGTGTAA ATGGAGGAAATCTCCGCAAGACTGAGTGCCG TGCAGGACAAAATTCTGGACATCTATGAAGC CGACAAAAATGACCTGACATCACAGATCGAG CACTGGAAACTGATTAGGATGGAATGCGCCA TCATGTACACCGCTCGCCAGATGGGCATTTCT CACCTGTGTCATCAGGTGGTCCCCTCCCTGGT CGCATCTAAAACAAAGGCCTTCCAGGTCATC GAGCTGCAGATGGCTCTGGAAACTCTGAACG CAAGTCCCTACAAGACCGATGAGTGGACACT GCAGCAGACTAGCCTGGAGGTCTGGCTGTCC GAACCTCAGAAATGCTTTAAGAAAAAGGGGA TTACAGTGACTGTCCAGTATGACAACGATAA GGCAAATACAATGGACTACACAAACTGGAGC GAAATCTACATCATTGAGGAAACCACATGTA CCCTGGTGGCCGGAGAAGTGGATTACGTCG GCCTGTACTATATTCACGGGAACGAGAAGAC ATACTTCAAGTACTTCAAGGAAGACGCTAAA AAGTACTCCAAGACCCAGCTGTGGGAGGTGC ATGTCGGCAGCAGAGTGATCGTCTGCCCAAC CTCAATCCCCAGCGATCAGATTAGCACTACCG AAACTGCCGACCCTAAAACAACTGAGGCTAC CAACAATGAATCCACTCAGGGAACCAAGCGG AGAAGGCTGGACCTGCCAGACAGCCGGGAC AACACACAGTACAGTACAAAGTATACTGATT GTGCAGTGGACTCACGCCCCCGAGGCGGAG GACTGCACAGCACCACAAACTGCACCTACAA AGGAAGGAATGTGTGTAGCTCCAAGGTGAG TCCTATTGTCCATCTGAAAGGCGATCCAAACT CACTGAAGTGCCTGCGGTACAGACTGAAACC ATTCAAGGACCTGTATTGTAATATGTCTAGTA CTTGGCATTGGACCTCCGACGATAAAGGCGA TAAAGTGGGGATCGTGACCGTCACATATACT ACCGAGACCCAGCGGCAGCTGTTTCTGAATA CAGTGAAGATTCCCCCTACAGTCCAGATCAGT ACTGGCGTGATGTCACTG ATGTATGTCCTGCACCTGTACCTGGTCATCAA GTATCCACTGCTGAAACTGCTGACCCAGAGA CCCCCACGCCCTCCAACAACTAAAGTGCATCG GGGCCAGAGCGACGATGACTCCATCTACCAG ACCCCAGAAACCACACCCTCTACACCTCAGAG TATTCAGACAGCCCCCTGGACTGTCGATCACG AGGAAGAGGACTATACTGTGCAGCTGACTGT CCATACCAAGGGCGGGACATGCGTGGTCCTG AAATTCCACCTGTCCTGTATC ATGATTCTGCCCATTTTTGTGGTGTGCTTTATT CTGTTCCTGTGCCTGTGTATCTTTCTGCGGCC CCTGGTCCTGTCTATTTCTATTTACGCCTGGCT GCTGGTGCTGGTCCTGCTGCTGTGGGTGAGC GTCGGCTCCGCTCTGCGGATCTTCTTTTGCTA CCTGATCTTCCTGTATATTCCCATGATGTGTAT TAACTTTCACGCCCAGTATCTGACCCAGCAGG AC ATGTTCCAGGATGCCGAAGAAAAACCCCGAA CTCTGCACGATCTGTGTCAGGCTCTGGAGAC CTCTGTCCATGAGATTGAACTGAAATGCGTG GAGTGTAAGAAAACACTGCAGCGGAGCGAA GTGTACGACTTCGTCTTTGCCGATCTGCGCAT CGTCTATCGAGACGGAAACCCATTCGCTGTG TGCAAGGTCTGTCTGCGCCTGCTGAGCAAAA TTTCCGAGTACCGGCACTACAACTATAGTCTG TATGGCGATACCCTGGAGCAGACACTGAAGA AATGCCTGAATGAAATCCTGATTAGGTGCAT CATTTGTCAGCGCCCCCTGTGTCCTCAGGAAA AGAAACGACACGTGGACCTGAACAAGAGGT TTCATAATATCTCCGGCCGGTGGACTGGAAG ATGCGCAGTGTGTTGGAGGCCCCGGAGAAG GCAGACCCAGGTC MEEISARLSAVQDKILDIYEADKNDL TSQIEHWKLIRMECAIMYTARQMGI SHLCHQVVPSLVASKTKAFQVIELQ MALETLNASPYKTDEWTLQQTSLEV WLSEPQKCFKKKGITVTVQYDNDKA NTMDYTNWSEIYIIEETTCTLVAGEV DYVGLYYIHGNEKTYFKYFKEDAKKY SKTQLWEVHVGSRVIVCPTSIPSDQI STTETADPKTTEATNNESTQGTKRRR LDLPDSRDNTQYSTKYTDCAVDSRP RGGGLHSTTNCTYKGRNVCSSKVSPI VHLKGDPNSLKCLRYRLKPFKDLYCN MSSTWHWTSDDKGDKVGIVTVTYT TETQRQLFLNTVKIPPTVQISTGVMS L HPV58 E4 HPV58 E5 HPV58 E6 Page 46 of 48 % Nucleotide Change 26.6% LYVLHLYLVIKYPLLKLLTQRPPRPPTT KVHRGQSDDDSIYQTPETTPSTPQSI QTAPWTVDHEEEDYTVQLTVHTKG GTCVVLKFHLSCI 22.1% MILPIFVVCFILFLCLCIFLRPLVLSISIY AWLLVLVLLLWVSVGSALRIFFCYLIF LYIPMMCINFHAQYLTQQD 25.5% MFQDAEEKPRTLHDLCQALETSVHEI ELKCVECKKTLQRSEVYDFVFADLRIV YRDGNPFAVCKVCLRLLSKISEYRHY NYSLYGDTLEQTLKKCLNEILIRCIICQ RPLCPQEKKRHVDLNKRFHNISGRW TGRCAVCWRPRRRQTQV 27.7% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV58 E7 ATGAGAGGAAACAACCCAACGCTAAGAGAA TATATTTTAGATTTACATCCTGAACCAACTGA CCTATTCTGCTATGAGCAATTATGTGACAGCT CAGACGAGGATGAAATAGGCTTGGACGGGC CAGATGGACAAGCACAACCGGCCACAGCTAA TTACTACATTGTAACTTGTTGTTACACTTGTG GCACCACGGTTCGTTTGTGTATCAACAGTACA ACAACCGACGTACGAACCCTACAGCAGCTGC TTATGGGCACATGTACCATTGTGTGCCCTAGC TGTGCACAGCAATAA ATGTCCGTGTGGCGGCCTAGTGAGGCCACTG TGTACCTGCCTCCTGTGCCTGTGTCTAAGGTT GTAAGCACTGATGAATATGTGTCACGCACAA GCATTTATTATTATGCTGGCAGTTCCAGACTT TTGGCTGTTGGCAATCCATATTTTTCCATCAA AAGTCCCAATAACAATAAAAAAGTATTAGTTC CCAAGGTATCAGGCTTACAGTATAGGGTCTT TAGGGTGCGTTTACCTGATCCCAATAAATTTG GTTTTCCTGATACATCTTTTTATAACCCTGATA CACAACGTTTGGTCTGGGCATGTGTAGGCCT TGAAATAGGTAGGGGACAGCCATTGGGTGTT GGCGTAAGTGGTCATCCTTATTTAAATAAATT TGATGACACTGAAACCAGTAACAGATATCCC GCACAGCCAGGGTCTGATAACAGGGAATGCT TATCTATGGATTATAAACAAACACAATTATGT TTAATTGGCTGTAAACCTCCCACTGGTGAGCA TTGGGGTAAAGGTGTTGCCTGTAACAATAAT GCAGCTGCTACTGATTGTCCTCCATTGGAACT TTTTAATTCTATTATTGAGGATGGTGACATGG TAGATACAGGGTTTGGATGCATGGACTTTGG TACATTGCAGGCTAATAAAAGTGATGTGCCT ATTGATATTTGTAACAGTACATGCAAATATCC AGATTATTTAAAAATGGCCAGTGAACCTTATG GGGATAGTTTGTTCTTTTTTCTTAGACGTGAG CAGATGTTTGTTAGACACTTTTTTAATAGGGC TGGAAAACTTGGCGAGGCTGTCCCGGATGAC CTTTATATTAAAGGGTCCGGTAATACTGCAGT TATCCAAAGTAGTGCATTTTTTCCAACTCCTA GTGGCTCTATAGTTACCTCAGAATCACAATTA TTTAATAAGCCTTATTGGCTACAGCGTGCACA AGGTCATAACAATGGCATTTGCTGGGGCAAT CAGTTATTTGTTACCGTGGTTGATACCACTCG TAGCACTAATATGACATTATGCACTGAAGTAA CTAAGGAAGGTACATATAAAAATGATAATTT TAAGGAATATGTACGTCATGTTGAAGAATAT GACTTACAGTTTGTTTTTCAGCTTTGCAAAAT TACACTAACTGCAGAGATAATGACATATATAC ATACTATGGATTCCAATATTTTGGAGGACTG GCAATTTGGTTTAACACCTCCTCCGTCTGCCA GTTTACAGGACACATATAGATTTGTTACCTCC CAGGCTATTACTTGCCAAAAAACAGCACCCCC TAAAGAAAAGGAAGATCCATTAAATAAATAT ACTTTTTGGGAGGTTAACTTAAAGGAAAAGT TTTCTGCAGATCTAGATCAGTTTCCTTTGGGA CGAAAGTTTTTATTACAATCAGGCCTTAAAGC AAAGCCCAGACTAAAACGTTCGGCCCCTACT ACCCGTGCACCATCCACCAAACGCAAAAAGG TTAAAAAATAA ATGCGGGGAAATAATCCTACCCTGAGAGAGT ACATCCTGGACCTGCACCCTGAGCCCACCGA CCTGTTCTGTTACGAGCAGCTGTGTGACAGCT CCGACGAGGATGAAATCGGCCTGGACGGAC CAGATGGACAGGCACAGCCTGCAACCGCTAA CTACTATATCGTGACATGCTGTTACACTTGCG GCACCACAGTCCGGCTGTGTATTAATTCTACT ACCACAGATGTGAGAACACTGCAGCAGCTGC TGATGGGGACTTGCACCATTGTCTGCCCCAG CTGTGCCCAGCAG ATGGTGCTGATCCTGTGCTGTACCCTGGCTAT CCTGTTTTGTGTCGCCGATGTCAATGTGTTTC ATATTTTCCTGCAGATGTCCGTGTGGAGGCCC TCTGAGGCCACCGTCTATCTGCCCCCTGTGCC TGTCTCAAAAGTGGTCAGCACCGACGAATAC GTGAGCAGGACATCCATCTACTATTACGCAG GAAGCTCCCGCCTGCTGGCTGTCGGCAACCC TTATTTCAGCATCAAGAGTCCAAACAATAACA AGAAAGTGCTGGTCCCCAAGGTGAGTGGGC TGCAGTATCGGGTGTTTAGGGTCCGCCTGCC AGATCCCAACAAGTTTGGATTCCCCGACACTT CCTTCTACAATCCTGATACCCAGCGACTGGTG TGGGCTTGCGTCGGACTGGAGATCGGACGA GGACAGCCACTGGGAGTGGGCGTCTCAGGA CACCCCTATCTGAACAAATTTGACGATACAGA GACTAGCAATAGATACCCCGCACAGCCTGGC AGTGACAACAGAGAATGTCTGTCAATGGATT ACAAGCAGACTCAGCTGTGCCTGATTGGCTG TAAACCACCCACCGGGGAGCATTGGGGGAA GGGAGTGGCTTGCAATAACAATGCCGCTGCA ACTGACTGTCCTCCACTGGAGCTGTTCAATAG CATCATTGAAGACGGCGATATGGTGGACACC GGCTTTGGGTGCATGGATTTCGGGACACTGC AGGCCAACAAGTCTGACGTGCCTATCGATAT TTGCAACAGTACCTGTAAGTACCCTGACTACC TGAAGATGGCTTCCGAGCCCTACGGCGACTC TCTGTTCTTTTTCCTGCGGAGAGAACAGATGT TTGTGAGACACTTTTTCAACAGGGCAGGGAA ACTGGGAGAGGCCGTCCCTGACGATCTGTAC ATCAAGGGAAGCGGCAATACCGCTGTGATTC AGTCTAGTGCATTTTTCCCTACACCATCAGGC AGCATCGTGACTTCCGAATCTCAGCTGTTTAA CAAGCCATACTGGCTGCAGCGAGCACAGGG ACATAACAATGGGATTTGCTGGGGAAACCAG CTGTTCGTGACAGTGGTGGACACCACAAGAT CCACTAATATGACCCTGTGTACAGAGGTCACT AAGGAAGGCACTTACAAGAACGACAACTTCA AGGAGTACGTGAGACACGTCGAGGAATACG ATCTGCAGTTTGTGTTCCAGCTGTGCAAGATC ACCCTGACAGCAGAGATCATGACCTACATTC ATACAATGGACTCTAATATTCTGGAAGATTG GCAGTTTGGGCTGACCCCCCCTCCAAGTGCCT CACTGCAGGACACATATAGGTTCGTGACTAG CCAGGCAATCACTTGTCAGAAAACCGCCCCC CCTAAGGAGAAAGAAGATCCCCTGAACAAGT ACACATTTTGGGAAGTGAATCTGAAGGAAAA ATTCTCCGCTGACCTGGATCAGTTTCCACTGG GGCGCAAGTTCCTGCTGCAGTCTGGACTGAA GGCAAAACCCCGACTGAAACGGAGCGCACC AACTACCCGCGCTCCCTCCACCAAGCGAAAG AAAGTGAAGAAA MRGNNPTLREYILDLHPEPTDLFCYE QLCDSSDEDEIGLDGPDGQAQPATA NYYIVTCCYTCGTTVRLCINSTTTDVR TLQQLLMGTCTIVCPSCAQQ HPV58 L1 Page 47 of 48 MSVWRPSEATVYLPPVPVSKVVSTD EYVSRTSIYYYAGSSRLLAVGNPYFSIK SPNNNKKVLVPKVSGLQYRVFRVRL PDPNKFGFPDTSFYNPDTQRLVWAC VGLEIGRGQPLGVGVSGHPYLNKFD DTETSNRYPAQPGSDNRECLSMDYK QTQLCLIGCKPPTGEHWGKGVACN NNAAATDCPPLELFNSIIEDGDMVD TGFGCMDFGTLQANKSDVPIDICNS TCKYPDYLKMASEPYGDSLFFFLRRE QMFVRHFFNRAGKLGEAVPDDLYIK GSGNTAVIQSSAFFPTPSGSIVTSESQ LFNKPYWLQRAQGHNNGICWGNQ LFVTVVDTTRSTNMTLCTEVTKEGTY KNDNFKEYVRHVEEYDLQFVFQLCKI TLTAEIMTYIHTMDSNILEDWQFGLT PPPSASLQDTYRFVTSQAITCQKTAP PKEKEDPLNKYTFWEVNLKEKFSADL DQFPLGRKFLLQSGLKAKPRLKRSAP TTRAPSTKRKKVKK % Nucleotide Change 22.9% 30.1% Gene PaVE DNA Sequence Optimized DNA Sequence Protein Sequence HPV58 L2 ATGAGACACAAACGGTCTACAAGGCGCAAGC GTGCATCTGCTACACAACTTTACCAAACATGC AAGGCCTCAGGCACCTGCCCACCTGATGTTAT ACCCAAAGTTGAAGGCACTACTATAGCAGAT CAAATATTACGATATGGTAGCTTAGGGGTGT TTTTTGGAGGTTTAGGCATTGGTACAGGGTC GGGTACAGGTGGCAGGACTGGATATGTGCC CCTTGGTAGTACCCCACCGTCTGAGGCTATAC CTTTACAGCCCATACGTCCCCCAGTTACCGTT GATACTGTGGGGCCTTTGGATTCTTCTATTGT ATCTTTAATAGAGGAATCTAGTTTTATAGACG CCGGTGCACCAGCCCCATCAATTCCCACTCCA TCTGGTTTTGATATTACCACCTCTGCAGATAC TACACCTGCAATACTTAATGTTTCCTCTATTG GAGAATCATCTATACAAACTGTTTCTACACAT TTAAATCCCTCCTTTACTGAGCCATCCGTACTC CGCCCTCCTGCACCTGCAGAGGCCTCTGGAC ATTTAATATTTTCCTCTCCTACTGTTAGCACAC ATAGTTATGAAAACATACCAATGGATACCTTT GTTATTTCTACTGACAGTGGCAATGTCACGTC TAGCACACCCATTCCAGGGTCTCGCCCTGTG GCACGCCTTGGTTTATACAGTCGCAACACCCA ACAAGTTAAGGTTGTTGACCCTGCTTTTTTAA CATCTCCTCATAGACTTGTAACATATGATAAT CCAGCATTTGAAGGCTTTAACCCTGAGGACA CATTGCAGTTTCAACATAGTGACATATCGCCT GCTCCTGATCCTGATTTTCTAGATATTGTTGC ATTACACAGACCTGCATTAACCTCTCGCAGGG GTACTGTACGTTATAGTAGGGTTGGGCAAAA GGCTACACTTCGTACTCGCAGTGGAAAGCAA ATAGGGGCTAAAGTACATTACTACCAAGACT TAAGTCCCATACAGCCTGTCCAGGAACAGGT ACAACAGCAGCAACAATTTGAATTACAATCTT TAAATACTTCTGTTTCTCCCTATAGTATTAATG ATGGACTTTATGATATTTATGCTGACGATGCT GATACTATACATGATTTTCAGAGTCCTCTGCA CTCACATACGTCCTTTGCCACCACACGTACCA GTAATGTGTCCATACCATTAAATACTGGATTT GACACTCCTCTTGTGTCATTGGAACCTGGTCC AGACATTGCATCTTCTGTAACATCTATGTCTA GTCCATTTATTCCTATATCTCCACTAACTCCTT TTAATACCATAATTGTGGATGGTGCTGATTTT ATGTTGCACCCTAGCTATTTTATTTTGCGTCG CAGACGTAAACGTTTTCCATATTTTTTTGCAG ATGTCCGTGTGGCGGCCTAG ATGAGGCATAAGAGGAGCACCAGAAGAAAA AGAGCATCCGCAACCCAGCTGTATCAGACCT GTAAAGCATCCGGCACCTGTCCACCTGACGT GATCCCCAAGGTCGAGGGCACCACAATCGCC GATCAGATTCTGAGATACGGATCTCTGGGCG TGTTCTTTGGAGGACTGGGAATTGGAACCGG CAGTGGGACAGGAGGCCGGACTGGATATGT GCCACTGGGGAGTACCCCCCCTTCAGAAGCC ATCCCACTGCAGCCCATTAGACCACCCGTGAC TGTGGACACCGTGGGCCCTCTGGATAGCTCC ATCGTCAGCCTGATTGAGGAATCTAGTTTCAT CGACGCAGGAGCACCAGCTCCTAGCATCCCA ACACCATCCGGGTTTGACATTACTACCTCTGC TGATACAACTCCAGCAATTCTGAACGTGTCAA GCATCGGGGAGTCCTCTATTCAGACAGTCAG CACTCACCTGAATCCTTCTTTCACCGAGCCAA GTGTGCTGAGACCTCCAGCACCAGCAGAAGC TAGCGGACACCTGATCTTCAGTTCACCCACCG TGAGTACACATTCATACGAAAACATCCCTATG GACACATTTGTGATTAGCACTGATTCCGGAA ATGTCACTAGCTCCACCCCTATCCCAGGAAGC CGGCCTGTGGCAAGACTGGGACTGTACTCCC GAAACACTCAGCAGGTCAAGGTGGTGGACCC CGCTTTTCTGACTTCCCCTCATCGCCTGGTGA CCTATGATAACCCAGCATTCGAGGGCTTTAAT CCCGAAGACACCCTGCAGTTCCAGCACTCTG ATATCAGTCCCGCCCCTGACCCAGATTTTCTG GACATTGTGGCCCTGCATAGGCCCGCTCTGA CCTCACGGAGAGGGACAGTGCGCTACAGCC GAGTCGGACAGAAAGCAACACTGAGAACTA GGAGCGGGAAGCAGATCGGAGCCAAAGTGC ACTACTATCAGGATCTGTCCCCTATTCAGCCA GTGCAGGAGCAGGTCCAGCAGCAGCAGCAG TTCGAACTGCAGTCCCTGAACACCTCCGTGTC TCCTTATTCTATCAATGACGGCCTGTACGATA TCTACGCAGACGATGCCGACACAATCCATGA TTTCCAGTCCCCACTGCACTCACATACCAGCT TCGCAACCACACGCACTTCCAACGTGTCTATC CCTCTGAATACCGGATTTGACACACCACTGGT GTCTCTGGAGCCCGGCCCTGATATTGCTTCTA GTGTCACCAGTATGTCAAGCCCCTTCATCCCT ATTTCACCACTGACTCCCTTTAATACCATCATT GTGGACGGCGCCGATTTCATGCTGCACCCAA GCTACTTTATCCTGAGGCGCCGACGGAAAAG GTTCCCCTATTTCTTTGCTGACGTGCGCGTCG CCGCT MRHKRSTRRKRASATQLYQTCKASG TCPPDVIPKVEGTTIADQILRYGSLGV FFGGLGIGTGSGTGGRTGYVPLGSTP PSEAIPLQPIRPPVTVDTVGPLDSSIV SLIEESSFIDAGAPAPSIPTPSGFDITTS ADTTPAILNVSSIGESSIQTVSTHLNP SFTEPSVLRPPAPAEASGHLIFSSPTV STHSYENIPMDTFVISTDSGNVTSST PIPGSRPVARLGLYSRNTQQVKVVD PAFLTSPHRLVTYDNPAFEGFNPEDT LQFQHSDISPAPDPDFLDIVALHRPA LTSRRGTVRYSRVGQKATLRTRSGK QIGAKVHYYQDLSPIQPVQEQVQQ QQQFELQSLNTSVSPYSINDGLYDIY ADDADTIHDFQSPLHSHTSFATTRTS NVSIPLNTGFDTPLVSLEPGPDIASSV TSMSSPFIPISPLTPFNTIIVDGADFM LHPSYFILRRRRKRFPYFFADVRVAA Page 48 of 48 % Nucleotide Change 28.5%