6 8 4 6 8 3.0 2.0 1.0 2 3 4 5 6 7 2 embryo 8-12hr 2 4 6 8 embryo 20-24hr 4 6 8 2 4 6 8 1.2 0.8 4 3 2 5 4 3 2 2 1.6 embryo 16-20hr median H3K9me3 density embryo 12-16hr 5 distance to TE (kb) median H3K9me3 density distance to TE (kb) 6 distance to TE (kb) 2 4 6 8 pupae 2 4 6 8 4 6 8 distance to TE (kb) 1.2 0.8 5.0 4.0 2 1.6 L2 larvae median H3K9me3 density L1 larvae 6.0 distance to TE (kb) median H3K9me3 density distance to TE (kb) 1.2 distance to TE (kb) distance to TE (kb) embryo 4-8hr median H3K9me3 density 4 median H3K9me3 density 2.0 1.0 2 0.8 median H3K9me3 density median H3K9me3 density median H3K9me3 density embryo 0-4hr 2 4 6 8 distance to TE (kb)